##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937819_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1621905 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.181783766620114 33.0 31.0 34.0 31.0 34.0 2 32.47398275484692 34.0 31.0 34.0 31.0 34.0 3 32.55796116295344 34.0 31.0 34.0 31.0 34.0 4 36.18353294428465 37.0 35.0 37.0 35.0 37.0 5 36.09942690848108 37.0 35.0 37.0 35.0 37.0 6 36.14462807624368 37.0 35.0 37.0 35.0 37.0 7 36.088962670440004 37.0 35.0 37.0 35.0 37.0 8 36.06663892151513 37.0 35.0 37.0 35.0 37.0 9 37.7625156837176 39.0 37.0 39.0 35.0 39.0 10-11 37.69010546240378 39.0 37.0 39.0 35.0 39.0 12-13 37.583738874964936 39.0 37.0 39.0 35.0 39.0 14-15 38.81272916724469 40.0 38.0 41.0 35.0 41.0 16-17 38.77083676294234 40.0 38.0 41.0 35.0 41.0 18-19 38.76110037270987 40.0 38.0 41.0 35.0 41.0 20-21 38.673688039681736 40.0 38.0 41.0 34.0 41.0 22-23 38.61837006483117 40.0 38.0 41.0 34.0 41.0 24-25 38.64033405162448 40.0 38.0 41.0 34.0 41.0 26-27 38.50756980217707 40.0 38.0 41.0 34.0 41.0 28-29 38.35589538228194 40.0 38.0 41.0 34.0 41.0 30-31 38.394005505871185 40.0 38.0 41.0 34.0 41.0 32-33 38.314478961468154 40.0 38.0 41.0 34.0 41.0 34-35 38.179028364793254 40.0 38.0 41.0 34.0 41.0 36-37 38.031891202012446 40.0 37.0 41.0 33.5 41.0 38-39 37.899006723575056 40.0 37.0 41.0 33.0 41.0 40-41 37.71098091441854 40.0 37.0 41.0 33.0 41.0 42-43 37.47775424577888 40.0 37.0 41.0 33.0 41.0 44-45 37.15310761111162 40.0 37.0 41.0 32.0 41.0 46-47 36.74637386283413 40.0 36.0 41.0 31.0 41.0 48-49 36.38882795231534 39.5 36.0 41.0 30.0 41.0 50-51 36.11350911428228 39.0 36.0 41.0 30.0 41.0 52-53 35.87777120114927 39.0 35.0 40.5 29.0 41.0 54-55 35.64833482848872 39.0 35.0 40.0 28.0 41.0 56-57 35.38158061045499 38.5 35.0 40.0 28.0 41.0 58-59 35.124155545485095 38.0 35.0 40.0 27.5 41.0 60-61 34.801990252203424 38.0 34.0 40.0 26.5 41.0 62-63 34.43262891476381 37.5 34.0 40.0 26.0 41.0 64-65 34.024328490262995 37.0 34.0 40.0 26.0 41.0 66-67 33.60312965309312 36.5 33.0 39.0 25.0 40.5 68-69 33.16266396613858 36.0 33.0 39.0 23.0 40.0 70-71 32.7611281178614 35.5 32.0 38.5 22.5 40.0 72-73 32.27029049173657 35.0 32.0 37.5 21.5 40.0 74-75 31.79568100474442 35.0 31.0 37.0 20.0 39.0 76-77 30.576924357468535 34.0 29.5 36.0 20.0 38.5 78-79 31.158048714320508 34.0 31.0 36.0 20.0 38.5 80-81 30.963747260166286 34.0 31.0 36.0 18.5 37.5 82-83 30.598741911517628 34.0 31.0 35.0 17.5 37.0 84-85 30.220617113826027 34.0 30.5 35.0 13.5 36.5 86-87 29.852718870710675 34.0 30.0 35.0 7.5 36.0 88-89 29.43006372136469 34.0 29.5 35.0 4.5 36.0 90-91 28.8888042147968 34.0 29.0 35.0 2.0 35.0 92-93 28.464020704048636 34.0 29.0 35.0 2.0 35.0 94-95 27.999745361164802 33.0 28.5 35.0 2.0 35.0 96-97 27.434593271492474 33.0 27.0 35.0 2.0 35.0 98-99 26.555849448642185 33.0 25.5 34.5 2.0 35.0 100-101 23.79803471843295 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2831713899557613 1101 2 0.24548444439398764 1101 3 0.23813526110743766 1101 4 0.1332429689825929 1101 5 0.07363789016038425 1101 6 0.09679148029491103 1101 7 0.1336968203608464 1101 8 0.08522810561675698 1101 9 0.09578845764577437 1101 10-11 0.24478658378993856 1101 12-13 0.26416196433387995 1101 14-15 0.2920010032582354 1101 16-17 0.2818450508680712 1101 18-19 0.2796912218043417 1101 20-21 0.3056857507028852 1101 22-23 0.20612829739055627 1101 24-25 0.26069142428548986 1101 26-27 0.09339432583259111 1101 28-29 0.05793733032707138 1101 30-31 0.2000905327500888 1101 32-33 0.2839611783107685 1101 34-35 0.24027908197480485 1101 36-37 0.28258962953700717 1101 38-39 0.3191618445303348 1101 40-41 0.3022009854710461 1101 42-43 0.33536889446379803 1101 44-45 0.15940167550472495 1101 46-47 0.010901372942448972 1101 48-49 0.11825802045238021 1101 50-51 0.2688628269917288 1101 52-53 0.22013021816207612 1101 54-55 0.1095074467527013 1101 56-57 0.08001676654357937 1101 58-59 0.15044832837061506 1101 60-61 0.364607454783048 1101 62-63 0.22755974869885875 1101 64-65 0.2041958505803052 1101 66-67 0.08735999159429042 1101 68-69 0.12520981993523606 1101 70-71 0.03422950104526734 1101 72-73 0.004063767926176354 1101 74-75 0.04917538448641423 1101 76-77 0.12526576960242508 1101 78-79 0.09943590002695046 1101 80-81 0.2628207382046881 1101 82-83 0.2681397575355682 1101 84-85 0.3363198469854467 1101 86-87 0.3576632689948589 1101 88-89 0.3685599594321438 1101 90-91 0.094783930354982 1101 92-93 -0.24282952950034797 1101 94-95 -0.3369812546317945 1101 96-97 -0.35483151003200675 1101 98-99 -0.15634307300149786 1101 100-101 -0.3374722460026547 1102 1 0.3321521458559218 1102 2 0.24125815636600123 1102 3 0.13085927445711576 1102 4 0.1252345405075701 1102 5 0.0699131331217373 1102 6 0.11825861337391785 1102 7 0.11946860425033634 1102 8 0.1350925660061364 1102 9 0.15586946006505542 1102 10-11 0.1818929336083812 1102 12-13 0.2673639585220826 1102 14-15 0.3333755883761924 1102 16-17 0.25682445022445677 1102 18-19 0.14304439832029203 1102 20-21 0.2525372378512927 1102 22-23 0.2852215706778978 1102 24-25 0.21700084480487902 1102 26-27 0.09065685033530713 1102 28-29 0.21189443278629483 1102 30-31 0.21667003401940832 1102 32-33 0.28865083736150865 1102 34-35 0.1913734983396438 1102 36-37 0.16982262453776542 1102 38-39 0.37460052921080234 1102 40-41 0.371933303347582 1102 42-43 0.3574256420640225 1102 44-45 0.5076544081026313 1102 46-47 0.24853094467859904 1102 48-49 0.22616092083937644 1102 50-51 0.23605128099088546 1102 52-53 0.3430179348992013 1102 54-55 0.19919943556981679 1102 56-57 0.3415396418655092 1102 58-59 0.313441309232104 1102 60-61 0.20080508094621763 1102 62-63 0.19987397565252962 1102 64-65 0.19459302410314194 1102 66-67 0.37417747333488904 1102 68-69 0.32042100235479865 1102 70-71 0.2752231750983256 1102 72-73 0.009397268819057558 1102 74-75 0.1351124899754801 1102 76-77 0.1949427124898655 1102 78-79 0.24282556091396046 1102 80-81 0.36310283504034047 1102 82-83 0.3958152589517887 1102 84-85 0.42663903645627954 1102 86-87 0.1741603103063234 1102 88-89 0.10303286987270965 1102 90-91 0.3279774777054776 1102 92-93 0.421090193406652 1102 94-95 0.1367580892692679 1102 96-97 0.2758603502462762 1102 98-99 0.4359139541706938 1102 100-101 0.5589765243856739 1103 1 0.30086724442446666 1103 2 0.2213226643457844 1103 3 0.23103317575050397 1103 4 0.1612567834229992 1103 5 0.04417397918678034 1103 6 0.06777935668444712 1103 7 0.08819280913661487 1103 8 0.11714372001159745 1103 9 0.17271732287103703 1103 10-11 0.19640561342144025 1103 12-13 0.2830542908439284 1103 14-15 0.23343712945890616 1103 16-17 0.22909130645920328 1103 18-19 0.2546188219050691 1103 20-21 0.22587438017129102 1103 22-23 0.14896535414728618 1103 24-25 0.07051382718268684 1103 26-27 0.03744599207954735 1103 28-29 0.10890521055394942 1103 30-31 0.13971636167647006 1103 32-33 0.2060515417792459 1103 34-35 0.14224212355004084 1103 36-37 0.15572971659676682 1103 38-39 0.17025578752178205 1103 40-41 0.15606231343209487 1103 42-43 0.30168675250603627 1103 44-45 0.34304214629893437 1103 46-47 0.2817020288885317 1103 48-49 0.0751728853658662 1103 50-51 0.13926595802504949 1103 52-53 0.05636074663636492 1103 54-55 0.06591011610550623 1103 56-57 0.09568257580357908 1103 58-59 0.1527897081358205 1103 60-61 0.19868742144612384 1103 62-63 0.13408053631382444 1103 64-65 0.23327108052118462 1103 66-67 0.30442043576692157 1103 68-69 0.21717313132375438 1103 70-71 -0.0011343271855963621 1103 72-73 -0.013913142052125238 1103 74-75 -0.10876057383043403 1103 76-77 -0.020574159166866224 1103 78-79 0.1598584875963489 1103 80-81 0.14856680192859528 1103 82-83 0.1791147128664754 1103 84-85 0.39185701526499983 1103 86-87 0.335440247325856 1103 88-89 0.22202571113196612 1103 90-91 0.09888029567018819 1103 92-93 0.12379006973698026 1103 94-95 0.13431465859624936 1103 96-97 0.20238749441691084 1103 98-99 0.20091220634519402 1103 100-101 0.30331063224559784 1104 1 0.32247727264766723 1104 2 0.24368459421643252 1104 3 0.2252256803941961 1104 4 0.12334244244313197 1104 5 0.08314135120294708 1104 6 0.15229961608590514 1104 7 0.1499350884709969 1104 8 0.17334153129257857 1104 9 0.15653407641967476 1104 10-11 0.16352996603166758 1104 12-13 0.12481448430419562 1104 14-15 0.18574591613792535 1104 16-17 0.2490196192281644 1104 18-19 0.12287240972346325 1104 20-21 0.08650739716512845 1104 22-23 0.16004799111890833 1104 24-25 0.1563991552677635 1104 26-27 0.2304733103533394 1104 28-29 0.20782145202900182 1104 30-31 0.12473947642980932 1104 32-33 0.15776052787966677 1104 34-35 0.1156347302089955 1104 36-37 0.1730796857924588 1104 38-39 0.1236750656408887 1104 40-41 0.021897955168022065 1104 42-43 0.05041317491988906 1104 44-45 0.044305353357657395 1104 46-47 -0.06609196788200222 1104 48-49 0.041511382453670365 1104 50-51 0.024966921178183554 1104 52-53 0.016281654080565033 1104 54-55 0.014218030123849701 1104 56-57 0.14772633789292655 1104 58-59 0.021872938147971865 1104 60-61 0.10046300937820973 1104 62-63 0.01152640600162158 1104 64-65 0.012198476074608777 1104 66-67 -0.10671274792354524 1104 68-69 -0.03316033874690305 1104 70-71 -0.026424279450445454 1104 72-73 0.02070045209612914 1104 74-75 0.013693845781080682 1104 76-77 -0.0827060630574259 1104 78-79 -0.050058809367925505 1104 80-81 -0.11092683978581519 1104 82-83 -0.055791406973799695 1104 84-85 -0.02985917643151126 1104 86-87 -0.07705232046282617 1104 88-89 -0.06766060730006629 1104 90-91 -0.13745526694244958 1104 92-93 -0.13489344173897067 1104 94-95 -0.15564252264830358 1104 96-97 -0.08700768106009349 1104 98-99 0.13016984558296585 1104 100-101 0.0060326268283397155 1105 1 0.2784055734429529 1105 2 0.22239859450090904 1105 3 0.18995552919276548 1105 4 0.05044424160555394 1105 5 -0.05679944359020084 1105 6 0.08217457109968507 1105 7 0.11685673273762376 1105 8 0.08218345949982364 1105 9 0.05766844512817215 1105 10-11 0.21102491373309107 1105 12-13 0.0805313379321646 1105 14-15 0.040892673836658844 1105 16-17 0.07610977209062497 1105 18-19 0.16098588096044608 1105 20-21 -0.034675695080245816 1105 22-23 -0.046707722011724684 1105 24-25 0.024838923372747956 1105 26-27 0.027334632513863255 1105 28-29 0.026131535720068655 1105 30-31 -0.029304319385730082 1105 32-33 0.1531946055261173 1105 34-35 0.17915145458212578 1105 36-37 0.05919605523982341 1105 38-39 0.1472860295822258 1105 40-41 0.18827776010203934 1105 42-43 0.17148935031317336 1105 44-45 0.08699190655880784 1105 46-47 0.01018747840325318 1105 48-49 0.10676073259967467 1105 50-51 0.23879776153508914 1105 52-53 0.30613658127686705 1105 54-55 0.18597565725705323 1105 56-57 0.11598205627393554 1105 58-59 0.10555446721169659 1105 60-61 0.11202243939683143 1105 62-63 0.03028363124202116 1105 64-65 0.1575905984027628 1105 66-67 0.19830050690227097 1105 68-69 0.17707637707287915 1105 70-71 0.21026481111671558 1105 72-73 0.12932129152707006 1105 74-75 0.14731795476753362 1105 76-77 0.16058562043432545 1105 78-79 0.16966486784627577 1105 80-81 0.12712209949400233 1105 82-83 0.25679242013401193 1105 84-85 0.18217078319782942 1105 86-87 -0.013926988137566099 1105 88-89 0.14719071622555546 1105 90-91 0.36164357673269976 1105 92-93 0.44559299175349665 1105 94-95 0.26909565039089145 1105 96-97 0.30441586909488905 1105 98-99 0.39999265953905194 1105 100-101 0.21939809249419184 1106 1 0.0794589039399014 1106 2 0.1270509116346119 1106 3 0.06440356209483866 1106 4 0.020007889455378347 1106 5 -0.012680753742529305 1106 6 -0.05420078586589483 1106 7 -0.03154103167475597 1106 8 -0.11741428944136345 1106 9 0.027564079028131516 1106 10-11 -0.09396712504803162 1106 12-13 -0.2784471119830698 1106 14-15 0.013181508146608678 1106 16-17 0.12968845278450658 1106 18-19 0.015983721439532417 1106 20-21 0.15252898237758217 1106 22-23 0.07329717719988338 1106 24-25 -0.009202539428692091 1106 26-27 0.1874437688988948 1106 28-29 0.061515446966986076 1106 30-31 0.13816813866477418 1106 32-33 -0.054141963865411924 1106 34-35 -0.37199534771534815 1106 36-37 -0.055090948312106036 1106 38-39 -0.05672584455960106 1106 40-41 -0.44252152048741067 1106 42-43 -0.23657952228798962 1106 44-45 0.04751940983032199 1106 46-47 -0.13265193704344114 1106 48-49 -0.15923906111773078 1106 50-51 -0.13796933896965413 1106 52-53 -0.028629449136921892 1106 54-55 -0.371796361798296 1106 56-57 -0.19255546459605455 1106 58-59 -0.07453829549240965 1106 60-61 -0.12600188905663146 1106 62-63 -0.4186839888370102 1106 64-65 -0.6407230413891511 1106 66-67 -0.5144719311646 1106 68-69 -0.23039782148700994 1106 70-71 -0.2889197280028384 1106 72-73 -0.18195269688242632 1106 74-75 -0.08809722118506969 1106 76-77 -0.03875911001233945 1106 78-79 -0.20643987214095816 1106 80-81 -0.5065989806532301 1106 82-83 -0.6717983474306273 1106 84-85 -0.8776253838137151 1106 86-87 -0.5874216086742869 1106 88-89 -0.5463039687744775 1106 90-91 -0.7270337650582164 1106 92-93 -0.7027193587721996 1106 94-95 -0.6562848443005542 1106 96-97 -0.7389212031104648 1106 98-99 -0.7439506901034996 1106 100-101 -1.1544198115457256 1107 1 0.3489156609752584 1107 2 0.27370736825911024 1107 3 0.19126088166424893 1107 4 0.16410201819027037 1107 5 0.18769623242973665 1107 6 0.17420718385739775 1107 7 0.17457910321560632 1107 8 0.2791943076104815 1107 9 0.208178743201195 1107 10-11 0.29415560162225773 1107 12-13 0.27994892966212603 1107 14-15 0.34764260935855873 1107 16-17 0.3430096126060249 1107 18-19 0.3382295871087635 1107 20-21 0.349482168352516 1107 22-23 0.32998647285423743 1107 24-25 0.32510025704294776 1107 26-27 0.3146029719265542 1107 28-29 0.30812846182553955 1107 30-31 0.28292493840559274 1107 32-33 0.26726820003521823 1107 34-35 0.19761455995249122 1107 36-37 0.2316394329446112 1107 38-39 0.2591068383744073 1107 40-41 0.40601765809505963 1107 42-43 0.3588929621392438 1107 44-45 0.2263448622873625 1107 46-47 0.18725617257706517 1107 48-49 0.14218420894279404 1107 50-51 0.08843221020789827 1107 52-53 -0.026623028591174602 1107 54-55 -0.017815917955985583 1107 56-57 0.02292305750033563 1107 58-59 0.13493937249469212 1107 60-61 0.15821770538871505 1107 62-63 0.08812311499617209 1107 64-65 0.07118073878078945 1107 66-67 0.09432760349309177 1107 68-69 0.38951986004803274 1107 70-71 0.1706278885904453 1107 72-73 0.16069975426399452 1107 74-75 0.14602444521161928 1107 76-77 0.08439093342942527 1107 78-79 0.1824117926358717 1107 80-81 0.14663264495237627 1107 82-83 0.1550420372130752 1107 84-85 0.3884664771137274 1107 86-87 0.20273257277976242 1107 88-89 0.1471775574304779 1107 90-91 0.3143021796897436 1107 92-93 0.3574474870610693 1107 94-95 0.3521421219915801 1107 96-97 0.5924658409350698 1107 98-99 0.4381255317882058 1107 100-101 0.651005255089526 1108 1 0.07079292142974936 1108 2 0.0023239163973940435 1108 3 0.060557374513159345 1108 4 0.04322096912452622 1108 5 0.04233244696969507 1108 6 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-0.14550109595718652 2316 60-61 0.1702008904440575 2316 62-63 0.0059642939952979646 2316 64-65 0.09925247705584894 2316 66-67 0.04580656997330124 2316 68-69 0.23939976013868858 2316 70-71 0.19658890326952871 2316 72-73 0.09931668581422315 2316 74-75 0.13134128590906968 2316 76-77 0.2338499812271806 2316 78-79 0.20819067094388188 2316 80-81 0.05213593968205288 2316 82-83 0.16977210680305177 2316 84-85 0.37196219343860193 2316 86-87 0.17338492255435156 2316 88-89 0.3360752326906642 2316 90-91 0.3986332342042864 2316 92-93 0.5729581342602863 2316 94-95 0.5172000370494985 2316 96-97 0.43472401601000144 2316 98-99 0.3560854225351058 2316 100-101 0.4612294479693091 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 8.0 4 14.0 5 24.0 6 65.0 7 150.0 8 363.0 9 706.0 10 1273.0 11 2028.0 12 3223.0 13 4711.0 14 6283.0 15 9041.0 16 12837.0 17 19069.0 18 16720.0 19 9360.0 20 7735.0 21 7446.0 22 7704.0 23 8556.0 24 9859.0 25 11705.0 26 14227.0 27 17568.0 28 21846.0 29 27656.0 30 34888.0 31 43808.0 32 57141.0 33 76229.0 34 106487.0 35 155954.0 36 245243.0 37 373565.0 38 286093.0 39 22314.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.963697045343544 20.078491541562034 15.335081426957478 21.622729986136942 2 15.461941359080832 21.205496006239578 40.01578390842867 23.31677872625092 3 19.344351241287253 25.46468504628816 30.23660448669928 24.9543592257253 4 12.828433231292832 17.567613392892927 37.17936623908306 32.42458713673119 5 13.401683506460103 38.24052788604412 33.85695931371854 14.500829293777242 6 31.13493083750281 37.23245196235291 17.034906483425356 14.59771071671892 7 28.08691014578536 34.00495096815165 20.258893091765547 17.649245794297446 8 26.38354280922742 35.53987440694738 19.406931971971233 18.66965081185396 9 26.650019575745805 17.185038581174606 20.480546024582207 35.684395818497386 10-11 24.894799633764002 27.587281622536462 26.68278968250298 20.835129061196554 12-13 25.857011392906525 24.968361601080254 27.879957517453143 21.29466948856008 14-15 22.694794084733694 26.24916379196069 26.55321982483561 24.502822298470008 16-17 21.435164205055166 29.78300208705195 27.205816616879535 21.576017091013345 18-19 21.33383274606096 27.735903150924372 30.359083916752216 20.57118018626245 20-21 22.174048418372223 26.78011350849772 30.630462326708408 20.415375746421645 22-23 21.8960190345479 26.63467935307693 30.47266373305761 20.99663787931756 24-25 21.73626343137149 26.986548204963 30.555967451804282 20.721220911861224 26-27 21.543988349351743 27.181413713927753 30.51885954852505 20.755738388195457 28-29 21.23745365737564 27.21693928121996 30.729175337122744 20.816431724281657 30-31 21.318326289147638 27.050752047746325 30.705929138882983 20.92499252422306 32-33 21.265487189446976 27.20513840206424 30.480546024582207 21.048828383906578 34-35 21.7977933356146 27.080316048103924 30.264411294126354 20.857479322155122 36-37 21.72849211266998 27.01246373862834 30.18302551629103 21.076018632410655 38-39 21.513035597029422 27.243426711182224 30.279455331847426 20.96408235994093 40-41 21.543672927566362 27.182274523295796 30.28337592249543 20.99067662664241 42-43 21.561337258417986 27.290581646734978 29.89795930951726 21.25012178532977 44-45 21.635607510920803 27.560307169655434 29.58178808253258 21.222297236891187 46-47 21.81277571744338 27.80295393380007 29.009868025562536 21.374402323194023 48-49 22.027245738807146 27.923706998868614 28.720023675862645 21.3290235864616 50-51 22.07361777465936 28.0525148058964 28.494341066320487 21.37952635312375 52-53 22.22590102379609 28.332516392760365 28.163610075805916 21.277972507637624 54-55 21.704825568992455 28.390710412836288 28.32698893028062 21.577475087890637 56-57 21.903009115823675 28.60648435019314 28.220456808506043 21.27004972547714 58-59 21.908003243099934 28.056020543743315 28.48739599421668 21.548580218940074 60-61 21.928472999343366 28.173413362681536 28.764693369833623 21.133420268141474 62-63 21.848258683461733 28.196441838455392 28.763367768149184 21.19193170993369 64-65 21.771805377010367 28.03706135686122 28.686575354290174 21.50455791183824 66-67 21.619361183299883 28.476760352795015 28.351999654727006 21.551878809178096 68-69 21.71286234397206 28.28131117420564 28.43899611876158 21.56683036306072 70-71 21.72463861940126 28.472074504980256 28.363837586048508 21.439449289569982 72-73 21.881830316818803 28.331067479291328 28.30554193987934 21.48156026401053 74-75 21.78980889756182 28.781895363785175 27.84472580083297 21.583569937820034 76-77 21.880165607726717 28.439735989469174 28.126030809449382 21.554067593354727 78-79 21.95082326030193 28.501638505337855 27.94685878642707 21.600679447933143 80-81 21.68733680456007 28.90640943828399 27.861095440238486 21.54515831691745 82-83 21.81252961430575 28.736412101041157 27.795474857011264 21.65558342764183 84-85 21.618443009437364 28.51428675362822 27.849081126539815 22.0181891103946 86-87 21.65003498971888 28.408229828504133 28.065700518834337 21.87603466294265 88-89 21.72679657563174 28.335475875590742 27.93742543490525 22.000302113872266 90-91 21.522659057379514 28.62377655581095 27.99964851753019 21.85391586927935 92-93 21.576466330287587 28.50854301534546 28.01257552575544 21.902415128611512 94-95 21.375265505686215 28.470594763565067 28.005616851788485 22.148522878960236 96-97 21.548611046886222 28.533360461925945 27.85089139006292 22.067137101124914 98-99 21.46529907721315 28.721505684296716 27.540623368984335 22.2725718695058 100-101 21.310096476268207 28.616164191884447 27.884752654878035 22.188986676969307 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 560.0 1 388.5 2 278.0 3 355.0 4 337.0 5 344.0 6 602.0 7 1239.5 8 1983.5 9 2453.5 10 2470.0 11 2399.5 12 2606.5 13 2905.0 14 3139.5 15 3333.5 16 3477.0 17 3612.5 18 3752.0 19 3883.5 20 4130.5 21 4579.5 22 5265.0 23 6214.0 24 7370.0 25 8928.0 26 11082.5 27 13461.5 28 15396.0 29 17950.0 30 21019.0 31 23862.0 32 27283.0 33 31083.0 34 36004.0 35 39775.5 36 43948.0 37 48328.0 38 50465.0 39 51167.5 40 50828.0 41 52571.0 42 55713.0 43 58136.0 44 59786.0 45 60871.5 46 59849.5 47 60033.5 48 64754.0 49 64414.0 50 59834.5 51 56791.5 52 53817.5 53 50747.0 54 47317.0 55 43491.5 56 38114.0 57 32896.5 58 29046.5 59 25615.5 60 21964.5 61 17750.5 62 13632.5 63 10210.0 64 7209.5 65 5178.0 66 3833.0 67 2928.0 68 2121.0 69 1368.5 70 985.0 71 756.5 72 587.5 73 460.5 74 365.0 75 271.0 76 183.5 77 118.0 78 74.0 79 46.5 80 25.0 81 18.0 82 13.0 83 9.0 84 6.5 85 3.0 86 1.0 87 1.5 88 2.5 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02028478856653133 2 0.0 3 0.0 4 0.0 5 0.03982970642546881 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0038534932687179584 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.39870707593848E-4 24-25 1.5413973074871834E-4 26-27 0.001294773738289234 28-29 0.001109806061390772 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 8.32354546043079E-4 42-43 0.012269522567597977 44-45 0.0 46-47 0.0 48-49 0.0 50-51 3.082794614974367E-5 52-53 0.0 54-55 1.84967676898462E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.090427614441043E-4 84-85 3.082794614974367E-5 86-87 0.0 88-89 0.0 90-91 0.012485318190646183 92-93 0.0015105693613374396 94-95 0.0 96-97 0.0 98-99 0.0015105693613374396 100-101 0.019390778128188766 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1621905.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.89385681669688 #Duplication Level Percentage of deduplicated Percentage of total 1 72.70858708867507 37.004204206353656 2 14.589729444436347 14.850552026789801 3 5.315181718390904 8.115302919915345 4 2.4197594174773815 4.926035572957908 5 1.3666875224147053 3.4777999539470112 6 0.8247611271643193 2.518516482832703 7 0.5528579603063941 1.9695951710263295 8 0.38310811606851997 1.5598279683604594 9 0.2897764658204868 1.327305776428468 >10 1.3833425671192279 12.770876763265706 >50 0.10521575614187509 3.685318518676548 >100 0.0580711275000962 5.276250130666093 >500 0.001947792323106933 0.6725395958671228 >1k 6.086851009709165E-4 0.45649670588198626 >5k 3.6521106058254993E-4 1.3893782070308824 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9243 0.5698854125241614 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8006 0.4936170737496956 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5176 0.31913089854214644 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2010 0.12392834352196955 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 9.2483838449231E-5 0.0 22-23 0.0 0.0 0.0 2.1579562304820566E-4 0.0 24-25 0.0 0.0 0.0 3.082794614974367E-4 0.0 26-27 0.0 0.0 0.0 6.47386869144617E-4 0.0 28-29 0.0 0.0 0.0 0.001572225253636927 0.0 30-31 0.0 0.0 0.0 0.0031752784534235974 0.0 32-33 0.0 0.0 0.0 0.004655019868611294 0.0 34-35 0.0 0.0 0.0 0.006843804045243094 0.0 36-37 0.0 0.0 0.0 0.01075895320626054 0.0 38-39 0.0 0.0 0.0 0.015753080482519013 0.0 40-41 0.0 0.0 0.0 0.021610390250970307 0.0 42-43 0.0 0.0 0.0 0.03156781685733751 0.0 44-45 0.0 0.0 0.0 0.03982970642546882 0.0 46-47 0.0 0.0 0.0 0.0554903030695386 0.0 48-49 0.0 0.0 0.0 0.07414121049013352 0.0 50-51 0.0 0.0 0.0 0.08453022834259713 0.0 52-53 0.0 0.0 0.0 0.10207132970180127 0.0 54-55 0.0 0.0 0.0 0.12787432062913673 0.0 56-57 0.0 0.0 0.0 0.17137255264642504 0.0 58-59 0.0 0.0 0.0 0.2266778880390652 0.0 60-61 0.0 0.0 0.0 0.291878994145773 0.0 62-63 0.0 0.0 0.0 0.3619200877979907 0.0 64-65 0.0 0.0 0.0 0.4401305871798903 0.0 66-67 0.0 0.0 0.0 0.5368070263054865 0.0 68-69 0.0 0.0 0.0 0.6540148775668119 0.0 70-71 0.0 0.0 0.0 0.7419978358781802 0.0 72-73 0.0 0.0 0.0 0.8467203689488595 0.0 74-75 0.0 0.0 0.0 0.964760574756228 0.0 76-77 0.0 0.0 0.0 1.101975763068737 0.0 78-79 0.0 0.0 0.0 1.2547282362407168 0.0 80-81 0.0 0.0 0.0 1.46602297915106 0.0 82-83 0.0 0.0 0.0 1.6937798453053663 0.0 84-85 0.0 0.0 0.0 1.9917627727887885 0.0 86-87 0.0 0.0 0.0 2.3567039993094543 0.0 88-89 0.0 0.0 0.0 2.774638465261529 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7205 0.0 36.211475 1 GTATCAA 10750 0.0 31.03391 1 TCAACGC 13100 0.0 25.388723 4 ATCAACG 13110 0.0 25.369358 3 TATCAAC 13255 0.0 25.091835 2 CAACGCA 13305 0.0 24.926119 5 AACGCAG 13650 0.0 24.357473 6 GAGTACT 8180 0.0 23.431345 12-13 GTACTTT 8705 0.0 22.070724 14-15 CGTATAG 110 0.0028696433 21.601564 1 GTACATG 7770 0.0 21.46812 1 AGTACTT 8540 0.0 21.219915 12-13 CGCAGAG 15515 0.0 21.094753 8 ACGCAGA 15585 0.0 21.030485 7 TACATGG 7980 0.0 20.779575 2 GTATAGG 530 0.0 19.726713 1 GCAGAGT 16360 0.0 19.71485 9 ACATGGG 7960 0.0 19.697678 3 ACTTTTT 10040 0.0 19.206984 16-17 GTGTAGC 620 0.0 19.16268 1 >>END_MODULE