FastQCFastQC Report
Thu 26 May 2016
SRR937818_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937818_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541540
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95840.6217159463912711No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75240.4880833452262024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49890.32363740155947945No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20760.1346705242809139No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19160.12429129312246194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55900.039.042841
GTATCAA91850.037.1694221
ATCAACG112600.030.0949673
TCAACGC113250.029.9251564
TATCAAC113700.029.762982
CAACGCA115350.029.2136295
AACGCAG117350.028.8617886
ACGCAGA132100.025.6031847
CGCAGAG132100.025.3874268
GTACATG72100.025.1263981
GCAGAGT138050.023.9491239
GAGTACT75650.023.70301812-13
TACATGG75500.023.4811172
GTACTTT80200.022.2087614-15
ACATGGG74650.022.156043
AGTACTT78100.022.07756612-13
AGAGTAC121300.020.8722410-11
CGGTATA1150.00371078820.6646962
ACAACCG4000.020.199745
CATGGGG29150.020.056344
Produced by FastQC (version 0.11.5)