##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937818_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1541540 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.761445048458036 33.0 31.0 34.0 30.0 34.0 2 31.881614489406697 33.0 31.0 34.0 30.0 34.0 3 31.918007317357965 34.0 31.0 34.0 30.0 34.0 4 35.50501316864953 37.0 35.0 37.0 33.0 37.0 5 35.48520570338752 37.0 35.0 37.0 33.0 37.0 6 35.570525578317785 37.0 35.0 37.0 33.0 37.0 7 35.51123227421929 37.0 35.0 37.0 33.0 37.0 8 35.49667345641372 37.0 35.0 37.0 33.0 37.0 9 37.115831571026376 39.0 37.0 39.0 34.0 39.0 10-11 37.006611894598905 39.0 37.0 39.0 33.0 39.0 12-13 36.88680994330345 39.0 37.0 39.0 33.0 39.0 14-15 38.03989225060654 40.0 38.0 41.0 33.0 41.0 16-17 37.988503379737146 40.0 38.0 41.0 33.0 41.0 18-19 37.96082943030995 40.0 38.0 41.0 33.0 41.0 20-21 37.79076734953358 40.0 38.0 41.0 32.5 41.0 22-23 37.66432236594574 40.0 37.5 41.0 32.0 41.0 24-25 37.52802943809437 40.0 37.0 41.0 32.0 41.0 26-27 37.62200040219521 40.0 37.0 41.0 32.0 41.0 28-29 37.55539428104363 40.0 37.0 41.0 32.0 41.0 30-31 37.33497184633548 40.0 37.0 41.0 32.0 41.0 32-33 37.08472274478768 39.0 36.0 41.0 31.0 41.0 34-35 36.81767972287453 39.0 36.0 41.0 30.5 41.0 36-37 36.84062171594639 39.0 36.0 41.0 31.0 41.0 38-39 36.79734324117441 39.0 36.0 41.0 31.0 41.0 40-41 36.59728745280693 39.0 35.5 41.0 30.0 41.0 42-43 36.33913456673197 39.0 35.0 41.0 30.0 41.0 44-45 36.06124168039753 39.0 35.0 41.0 29.5 41.0 46-47 35.63913067452029 39.0 35.0 41.0 27.5 41.0 48-49 35.2690478352816 39.0 35.0 40.5 26.0 41.0 50-51 34.361134968927175 38.0 34.0 39.5 24.0 40.5 52-53 34.45803903888319 38.0 34.0 39.5 24.0 40.5 54-55 34.769642694967374 39.0 35.0 40.0 24.0 41.0 56-57 34.52220409460669 38.0 34.0 40.0 23.0 41.0 58-59 34.3050517664154 38.0 34.0 40.0 23.0 41.0 60-61 34.00156304734227 38.0 34.0 40.0 22.0 41.0 62-63 33.64049878692736 37.5 33.0 40.0 20.5 41.0 64-65 33.28061224489796 37.0 33.0 40.0 19.5 41.0 66-67 32.87628605161073 36.5 32.5 39.0 18.0 41.0 68-69 32.40428564941552 36.0 32.0 39.0 14.0 40.5 70-71 31.962463186164484 35.5 31.0 39.0 9.5 40.0 72-73 31.492386185243326 35.0 31.0 38.0 7.5 40.0 74-75 31.014191652503342 35.0 31.0 37.0 6.5 39.0 76-77 30.560319874930265 35.0 30.0 37.0 2.0 39.0 78-79 30.03486026960053 34.0 29.5 36.0 2.0 39.0 80-81 29.507332926813447 34.0 29.0 36.0 2.0 37.5 82-83 29.007989088833245 34.0 29.0 35.0 2.0 37.0 84-85 28.545670238852054 34.0 28.5 35.0 2.0 36.5 86-87 28.0376714194896 34.0 27.0 35.0 2.0 36.0 88-89 27.565799784630954 33.0 26.0 35.0 2.0 36.0 90-91 27.05725897479144 33.0 25.5 35.0 2.0 35.0 92-93 26.52260272195337 33.0 25.0 35.0 2.0 35.0 94-95 25.914131323222232 32.0 23.5 34.5 2.0 35.0 96-97 25.171597233934897 32.0 19.5 34.0 2.0 35.0 98-99 24.25979377765092 32.0 7.5 34.0 2.0 35.0 100-101 21.753184153508826 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3837535509382022 1101 2 0.4717406203006682 1101 3 0.517886749188122 1101 4 0.4299456401503363 1101 5 0.39285959687644123 1101 6 0.3660682255636658 1101 7 0.37446563557050894 1101 8 0.4239224286692931 1101 9 0.4611460852008449 1101 10-11 0.389242519841126 1101 12-13 0.5091068519496318 1101 14-15 0.6930242690797499 1101 16-17 0.7635573141755287 1101 18-19 0.6969969828702247 1101 20-21 0.7278040625214572 1101 22-23 0.7788629671845868 1101 24-25 0.7337385335156341 1101 26-27 0.7992538835105094 1101 28-29 0.7505057082889977 1101 30-31 0.7025080956275787 1101 32-33 0.7757461873291831 1101 34-35 0.9418698676166386 1101 36-37 0.907540706824598 1101 38-39 0.9371654886658831 1101 40-41 1.0895590275011031 1101 42-43 1.0719634086976413 1101 44-45 1.133808345109145 1101 46-47 1.271878751358848 1101 48-49 1.3328581341735983 1101 50-51 1.335355726680568 1101 52-53 1.3640829727297827 1101 54-55 1.4564455748644676 1101 56-57 1.6248633141970643 1101 58-59 1.539678664531415 1101 60-61 1.551962584326013 1101 62-63 1.5397787766364885 1101 64-65 1.5318538888674667 1101 66-67 1.7072133993671272 1101 68-69 1.7133869975851184 1101 70-71 1.6506923426036444 1101 72-73 1.5867285310451713 1101 74-75 1.513143959245184 1101 76-77 1.5558179752356551 1101 78-79 1.7103509384663305 1101 80-81 1.7888871440139233 1101 82-83 1.9985643330516432 1101 84-85 2.097967862878569 1101 86-87 2.0832216080362294 1101 88-89 2.0029518622650464 1101 90-91 1.9731943568593735 1101 92-93 2.1614584505071086 1101 94-95 2.245725987654666 1101 96-97 2.165929696233274 1101 98-99 2.5714877302410812 1101 100-101 2.8219519540413422 1102 1 0.403288170704279 1102 2 0.5206749526988226 1102 3 0.4695897448525699 1102 4 0.33547336920663184 1102 5 0.38669189697422723 1102 6 0.408784227894877 1102 7 0.4155024968341223 1102 8 0.39381862292221115 1102 9 0.41728867866148533 1102 10-11 0.38795840533349235 1102 12-13 0.49108738159123533 1102 14-15 0.6914701733915223 1102 16-17 0.49750205774904543 1102 18-19 0.51663258477803 1102 20-21 0.44148394356839304 1102 22-23 0.585391476557426 1102 24-25 0.6238503510690023 1102 26-27 0.686418024000865 1102 28-29 0.6364856036625994 1102 30-31 0.6612961151953058 1102 32-33 0.6204762687408234 1102 34-35 0.7369480314107832 1102 36-37 0.8598532117798214 1102 38-39 0.8196606655863121 1102 40-41 0.8601547247716681 1102 42-43 1.0353091167479747 1102 44-45 1.0477401251349931 1102 46-47 1.3071322290530603 1102 48-49 1.3460351909197144 1102 50-51 1.2161912356736835 1102 52-53 1.1793673014419568 1102 54-55 1.3518351698092417 1102 56-57 1.4832177782058267 1102 58-59 1.2723155085473934 1102 60-61 1.3597836929318916 1102 62-63 1.4747553184571416 1102 64-65 1.4452508530049002 1102 66-67 1.4219146466217651 1102 68-69 1.3961926319991704 1102 70-71 1.4536714548788119 1102 72-73 1.2663032932989857 1102 74-75 1.1668955292671228 1102 76-77 1.3566087238248947 1102 78-79 1.4378981990823227 1102 80-81 1.4616995801176813 1102 82-83 1.5598490873910258 1102 84-85 1.6349858852698986 1102 86-87 1.6341593092876572 1102 88-89 1.6889957408138514 1102 90-91 1.8116592335735326 1102 92-93 1.8957759656231339 1102 94-95 1.9435897841659155 1102 96-97 2.2168363795541133 1102 98-99 2.6023189968714924 1102 100-101 2.2668624401882873 1103 1 0.2777186056448677 1103 2 0.3071216661176699 1103 3 0.23983428538525686 1103 4 0.249092060387909 1103 5 0.20798472790333733 1103 6 0.267535726750296 1103 7 0.2488116062414818 1103 8 0.20784064247092005 1103 9 0.1032085216904477 1103 10-11 0.1840795491872811 1103 12-13 0.35688440931551924 1103 14-15 0.4274608714039445 1103 16-17 0.31736688824211967 1103 18-19 0.3220357314660234 1103 20-21 0.22354309839561637 1103 22-23 0.2983034346984823 1103 24-25 0.35822955240616494 1103 26-27 0.0896610020903239 1103 28-29 0.33969406265755 1103 30-31 0.29651467556738 1103 32-33 0.36667553475586345 1103 34-35 0.4077430606611756 1103 36-37 0.5296544544672273 1103 38-39 0.6682108636169062 1103 40-41 0.6609177784397744 1103 42-43 0.7660710803394082 1103 44-45 0.8502527518564023 1103 46-47 0.9101481078365339 1103 48-49 1.1278109258130655 1103 50-51 0.8789033464254672 1103 52-53 0.747831383415722 1103 54-55 0.9396045095861396 1103 56-57 1.057052304567513 1103 58-59 0.9875947498540327 1103 60-61 1.083243959637322 1103 62-63 0.9644213916376216 1103 64-65 1.0781293821281182 1103 66-67 1.1462378906899247 1103 68-69 1.1686700129082936 1103 70-71 1.2204677195494291 1103 72-73 1.163483628315884 1103 74-75 1.0775274103760921 1103 76-77 1.1175076737177 1103 78-79 1.1599231981739244 1103 80-81 1.3685107425027674 1103 82-83 1.1658862953413198 1103 84-85 1.2387502833658957 1103 86-87 1.324472161291581 1103 88-89 1.395763300132188 1103 90-91 1.5481550004424705 1103 92-93 1.6387358871376136 1103 94-95 1.6056205969997528 1103 96-97 1.6096654918683448 1103 98-99 1.907297013658674 1103 100-101 2.094784754057695 1104 1 0.18536528866628643 1104 2 0.2749810948518707 1104 3 0.3293653145939075 1104 4 0.34337171952670076 1104 5 0.3086899600247577 1104 6 0.3268197267337527 1104 7 0.3012477686393069 1104 8 0.3535491161897468 1104 9 0.39768740461883567 1104 10-11 0.46452042416445494 1104 12-13 0.35961745712035764 1104 14-15 0.502404416783655 1104 16-17 0.5159216736022714 1104 18-19 0.42069110385966724 1104 20-21 0.508957771691648 1104 22-23 0.60153093691914 1104 24-25 0.5329976023710898 1104 26-27 0.6141660896548231 1104 28-29 0.4700269631868963 1104 30-31 0.5190866836498031 1104 32-33 0.45807956899327706 1104 34-35 0.5449537916783314 1104 36-37 0.538232719985082 1104 38-39 0.6326843448679469 1104 40-41 0.8450538085142298 1104 42-43 0.734213594790674 1104 44-45 0.877381848990261 1104 46-47 0.6454357767054404 1104 48-49 0.7347855609782243 1104 50-51 0.7518522308372511 1104 52-53 0.7935062887238473 1104 54-55 0.7362437679606586 1104 56-57 0.958666720545132 1104 58-59 1.1482937647275548 1104 60-61 1.1355068132950024 1104 62-63 1.0482531784403903 1104 64-65 1.01998437368578 1104 66-67 0.9825478595032209 1104 68-69 1.162272659848398 1104 70-71 1.0164787522094834 1104 72-73 1.030765774944829 1104 74-75 1.058340817883007 1104 76-77 1.064965723546699 1104 78-79 0.9525601032137381 1104 80-81 1.1063342152139555 1104 82-83 1.17573504837533 1104 84-85 1.1237311972422361 1104 86-87 0.9585129528205201 1104 88-89 1.069129809850775 1104 90-91 1.1485316403745784 1104 92-93 1.2465646551473917 1104 94-95 1.3773653033263926 1104 96-97 1.44449310212255 1104 98-99 1.6300585827927563 1104 100-101 1.6576062912663296 1105 1 0.2297922801793888 1105 2 0.2641967743532483 1105 3 0.3621089258684904 1105 4 0.3375933058788263 1105 5 0.3539773859379025 1105 6 0.2537267665316705 1105 7 0.268614380895535 1105 8 0.25925210051539693 1105 9 0.4092470581589609 1105 10-11 0.47137406678522353 1105 12-13 0.33801678535347435 1105 14-15 0.2975247802512442 1105 16-17 0.45472311988573466 1105 18-19 0.41456254993586583 1105 20-21 0.512141221958224 1105 22-23 0.49061230700704783 1105 24-25 0.4683321888821155 1105 26-27 0.4064343015025642 1105 28-29 0.28474230699324465 1105 30-31 0.39762836039151495 1105 32-33 0.38618242419096305 1105 34-35 0.4358356364496885 1105 36-37 0.4985820814736641 1105 38-39 0.533340598812913 1105 40-41 0.702045528874315 1105 42-43 0.5168047458823253 1105 44-45 0.6857051700911967 1105 46-47 0.8268845912215355 1105 48-49 0.6827255114498598 1105 50-51 0.704091527064719 1105 52-53 0.549251101466318 1105 54-55 0.9000075152843579 1105 56-57 1.0118957361516507 1105 58-59 0.9801111843161934 1105 60-61 0.9550058897411446 1105 62-63 0.9653774873451368 1105 64-65 0.9139328506226789 1105 66-67 1.0043383606842795 1105 68-69 1.1058771514415753 1105 70-71 1.03220743023682 1105 72-73 1.0732858365695144 1105 74-75 1.1388547107582951 1105 76-77 1.0549490424243153 1105 78-79 1.231401456067836 1105 80-81 1.2038551906576593 1105 82-83 1.1250311251084923 1105 84-85 1.0649523797554465 1105 86-87 0.7359912708157239 1105 88-89 0.6702586432587445 1105 90-91 0.8399550563745102 1105 92-93 0.9768486173410338 1105 94-95 1.013560763875475 1105 96-97 1.1224106812997867 1105 98-99 1.403034850504671 1105 100-101 1.3280322816608638 1106 1 0.17550508266043963 1106 2 0.3045784070222304 1106 3 0.24029241904283083 1106 4 0.12030444954992703 1106 5 0.23011662101304609 1106 6 0.20331993612104782 1106 7 0.21148188814883895 1106 8 0.209903745460565 1106 9 0.1963594735607188 1106 10-11 0.04662226773171341 1106 12-13 -0.025209404979186445 1106 14-15 0.08487566288139448 1106 16-17 0.10423196604475748 1106 18-19 0.021894787797442916 1106 20-21 0.30653619602646387 1106 22-23 0.002003239366040077 1106 24-25 0.09410281922280461 1106 26-27 0.06737078092488247 1106 28-29 0.3740490818737996 1106 30-31 0.38537575462730445 1106 32-33 0.33158459872840496 1106 34-35 0.26356159168380344 1106 36-37 0.3485498485055487 1106 38-39 0.46681941193048715 1106 40-41 0.37518018325108216 1106 42-43 0.2708632985404975 1106 44-45 0.2524605690247057 1106 46-47 0.6048432337348686 1106 48-49 0.625278844778471 1106 50-51 0.5596052085235712 1106 52-53 0.5330908245393786 1106 54-55 0.5039577736799998 1106 56-57 0.5073953542494891 1106 58-59 0.5215920711235142 1106 60-61 0.3229521355045861 1106 62-63 0.597706587013441 1106 64-65 0.4998737802512707 1106 66-67 0.7856429909554663 1106 68-69 0.8260609467335058 1106 70-71 0.7778995951079217 1106 72-73 0.8350872162398382 1106 74-75 0.7165097463705834 1106 76-77 0.8065780661377104 1106 78-79 0.764324117511034 1106 80-81 0.5222763142088596 1106 82-83 0.5585026689648309 1106 84-85 0.6430914417867157 1106 86-87 0.49481012579006034 1106 88-89 0.8079552945051205 1106 90-91 1.014908444453063 1106 92-93 1.1873330905536292 1106 94-95 0.8823264858691431 1106 96-97 0.7838001598065318 1106 98-99 1.0344779867972917 1106 100-101 1.036076410686352 1107 1 0.25459708476564913 1107 2 0.3123381537796597 1107 3 0.3461512516390144 1107 4 0.26207635528466966 1107 5 0.30825531073070067 1107 6 0.1928643680410147 1107 7 0.33302227222106495 1107 8 0.23191018765037796 1107 9 0.19071779863252658 1107 10-11 0.21003508492158574 1107 12-13 0.3254981709475402 1107 14-15 0.4860483451567319 1107 16-17 0.41585141896795363 1107 18-19 0.5226325373731342 1107 20-21 0.5291379825740066 1107 22-23 0.5702749143611143 1107 24-25 0.5891363857995628 1107 26-27 0.6463766093523233 1107 28-29 0.46229735878609546 1107 30-31 0.5435546141405396 1107 32-33 0.4126915214843834 1107 34-35 0.45262612625076315 1107 36-37 0.5407927769906564 1107 38-39 0.3985163542826129 1107 40-41 0.4693047332830034 1107 42-43 0.5301238820829184 1107 44-45 0.4042355667224484 1107 46-47 0.4700212994213331 1107 48-49 0.362471764234634 1107 50-51 0.40401593233615785 1107 52-53 0.6380364733860091 1107 54-55 0.6840929145855412 1107 56-57 0.7886163349419562 1107 58-59 0.6473451904820351 1107 60-61 0.8255437767092886 1107 62-63 0.7464181747750303 1107 64-65 0.7418167986142379 1107 66-67 0.9192808732128981 1107 68-69 1.0232942314837459 1107 70-71 0.7095566660568942 1107 72-73 0.7020795593870268 1107 74-75 0.8505442847806322 1107 76-77 0.8401256075126504 1107 78-79 0.7584408990502176 1107 80-81 0.9253975505413301 1107 82-83 0.9673890529578273 1107 84-85 1.1701407161529005 1107 86-87 1.2074692705664702 1107 88-89 1.1464151470889696 1107 90-91 1.172228911241728 1107 92-93 1.1500278623580122 1107 94-95 1.1839477342916496 1107 96-97 1.1309378303596738 1107 98-99 1.2949221079261015 1107 100-101 1.5141695183869892 1108 1 0.2164662478805326 1108 2 0.29157318707875746 1108 3 0.21112797268883554 1108 4 0.38860517334860845 1108 5 0.43133135291552094 1108 6 0.2879150115372866 1108 7 0.33973040321302506 1108 8 0.33360096827951935 1108 9 0.4256431425681271 1108 10-11 0.45955346175309586 1108 12-13 0.42549477125458424 1108 14-15 0.50319442817365 1108 16-17 0.4810611084507386 1108 18-19 0.5981498573176367 1108 20-21 0.6174611108641059 1108 22-23 0.5668719410015868 1108 24-25 0.6669458145199272 1108 26-27 0.5506021891192532 1108 28-29 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-1.514077370629316 2316 54-55 -1.4880169124351141 2316 56-57 -1.3480420226441439 2316 58-59 -1.4239748595912047 2316 60-61 -1.3875811567965215 2316 62-63 -1.4105632923626672 2316 64-65 -1.1539660529945976 2316 66-67 -1.2151790746656026 2316 68-69 -1.273338453131462 2316 70-71 -1.3714489149805082 2316 72-73 -1.4703936863347273 2316 74-75 -1.2328411667385168 2316 76-77 -0.8334269569506674 2316 78-79 -0.7376862805919728 2316 80-81 -0.6782836671250934 2316 82-83 -0.9681809377056432 2316 84-85 -1.0589766288124807 2316 86-87 -1.1045970257545008 2316 88-89 -1.028646276140197 2316 90-91 -0.9084758923481147 2316 92-93 -0.8482509946280281 2316 94-95 -0.552157093360389 2316 96-97 -0.30456007314694133 2316 98-99 -0.18507942330453986 2316 100-101 -0.08611912853210768 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6214.0 3 1205.0 4 1139.0 5 1233.0 6 1453.0 7 1689.0 8 2103.0 9 2623.0 10 3280.0 11 4325.0 12 5670.0 13 7442.0 14 8703.0 15 11254.0 16 14680.0 17 19856.0 18 16768.0 19 10694.0 20 10272.0 21 10920.0 22 11520.0 23 12752.0 24 14449.0 25 16201.0 26 18464.0 27 21698.0 28 25586.0 29 30797.0 30 37264.0 31 46663.0 32 59421.0 33 77250.0 34 105314.0 35 149834.0 36 216472.0 37 297275.0 38 231265.0 39 27789.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.70904760045297 18.92179832610931 15.196285160165047 22.172868913272676 2 14.888154265671675 20.024510569207234 41.698085469551735 23.389249695569365 3 18.755797057143862 24.917296402149578 30.683847853999367 25.643058686707192 4 12.234135628964344 16.715637902110004 38.161916662817916 32.888309806107735 5 13.196581663618995 37.21678932163444 34.78047330311031 14.80615571163625 6 30.651631663218005 36.52931169093733 17.99441843049074 14.824638215353932 7 27.6705664868533 33.47915137798052 21.02587869455366 17.824403440612514 8 25.906348436648884 34.74518761383388 20.20649677007539 19.141967179441846 9 26.28615894887869 16.898890487393732 21.067997709074575 35.746952854653 10-11 24.45916094699329 26.96241462745521 27.527924444403993 21.050499981147507 12-13 25.515661529850853 24.439761184055925 28.492670181062113 21.551907105031106 14-15 22.47084042312874 25.47795378347237 27.376507546467614 24.674698246931275 16-17 21.10135183663176 29.03161543453795 27.958485222807518 21.908547506022767 18-19 21.015670525391762 26.91032567275814 31.331333283283332 20.742670518566765 20-21 21.898711347325285 25.90212998964983 31.424634697594765 20.774523965430117 22-23 21.49355178171801 25.842759802325272 31.42333994372393 21.240348472232785 24-25 21.29916346547589 26.09282478274152 31.459645496558313 21.14836625522428 26-27 21.081980351443256 26.27949777849566 31.470970295768335 21.16755157429275 28-29 20.84121946106179 26.312087512046556 31.718468896722197 21.128224130169457 30-31 20.877065367609106 26.18104119619383 31.66644565006832 21.275447786128737 32-33 20.851317779516403 26.215041048255795 31.483233712584525 21.450407459643277 34-35 21.4120696144398 26.241599002573523 31.112308635370546 21.23402274761613 36-37 21.306127250582023 26.138891019249943 31.145806387876785 21.40917534229125 38-39 21.112146213501944 26.34251462294238 31.243560170227592 21.301778993328085 40-41 21.10219477824282 26.229365974974844 31.374375613907223 21.294063632875112 42-43 21.199499545040943 26.187118159365657 30.988983491485765 21.62439880410763 44-45 21.208404585903136 26.585739346562747 30.614848743062424 21.591007324471693 46-47 21.33776736108403 26.752422401264564 30.179369486713515 21.730440750937895 48-49 21.466865938660803 26.86719982109726 29.869996476552167 21.795937763689764 50-51 21.44509666263635 27.08019683555001 29.674779307565295 21.799927194248344 52-53 21.64732348944415 27.246929473800563 29.42071247911262 21.68503455764267 54-55 21.303052402104353 27.192943115683455 29.49579662612137 22.00820785609083 56-57 21.508217154209046 27.449151900786546 29.37198450634527 21.670646438659144 58-59 21.480249626165126 26.955167759414756 29.710920527352496 21.853662087067622 60-61 21.591411935102176 26.90680733190251 29.939998947349956 21.561781785645362 62-63 21.43301810223209 27.022180546647512 29.903383714424407 21.64141763669599 64-65 21.315023361297573 26.927810666268105 29.815936368894057 21.94122960354026 66-67 21.2519490427069 27.236009699157805 29.544352073060143 21.96768918507515 68-69 21.21387084154832 27.09646269236718 29.737616434047677 21.952050032036826 70-71 21.354307805381126 27.152440534096513 29.53084922697412 21.962402433548238 72-73 21.46310540875468 27.066322601775212 29.47989969178423 21.99067229768588 74-75 21.270383393018463 27.31943352202934 29.298206846350322 22.111976238601873 76-77 21.40682848971151 27.133098815344052 29.35612399342587 22.10394870151856 78-79 21.390891295531826 27.046527467271158 29.39051305514251 22.17206818205451 80-81 21.262200687355346 27.469844128187194 29.172993310031575 22.09496187442589 82-83 21.394274657637936 27.376262680407283 29.153517637404512 22.07594502455027 84-85 21.255456089444134 27.062496020417047 29.155139412100272 22.52690847803855 86-87 21.31244738959816 26.990683390236224 29.411988300388785 22.284880919776835 88-89 21.394174068372728 26.85017742651306 29.29145708071818 22.46419142439603 90-91 21.1863721151931 27.03066784199597 29.54753179221365 22.235428250597277 92-93 21.254303778097412 26.88699367961534 29.515218406280553 22.343484136006698 94-95 20.953587120280485 26.915902789984248 29.517637583090167 22.6128725066451 96-97 21.10945404567652 26.87675657058936 29.39182449402644 22.621964889707673 98-99 21.057367266119606 26.93772082236499 29.23727918575871 22.767632725756698 100-101 21.237811007341822 26.479751993351485 29.718854756135677 22.563582243171012 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2020.0 1 1206.5 2 431.5 3 473.0 4 386.5 5 350.0 6 626.0 7 1440.5 8 2575.0 9 3133.5 10 2779.0 11 2358.0 12 2419.5 13 2661.5 14 2932.0 15 3104.5 16 3210.5 17 3305.5 18 3316.5 19 3518.0 20 3937.0 21 4445.5 22 5104.0 23 5910.5 24 6941.0 25 8534.5 26 10697.5 27 12974.5 28 14942.5 29 17233.5 30 19898.0 31 22686.0 32 26057.5 33 30006.5 34 34366.5 35 37600.0 36 41315.0 37 45208.5 38 47088.5 39 47920.5 40 48451.5 41 50258.5 42 52727.5 43 54799.0 44 56196.0 45 57240.5 46 56882.0 47 56996.5 48 61251.0 49 61262.0 50 56688.0 51 53550.0 52 50521.0 53 48172.5 54 45452.0 55 41647.5 56 37054.5 57 31926.5 58 27624.5 59 24055.0 60 20328.0 61 16432.0 62 12694.0 63 9500.0 64 6913.0 65 5043.0 66 3656.0 67 2766.5 68 2035.5 69 1350.5 70 992.0 71 759.5 72 587.5 73 438.0 74 335.0 75 267.0 76 175.5 77 112.5 78 80.5 79 56.0 80 46.0 81 32.5 82 17.0 83 11.5 84 12.5 85 9.5 86 4.5 87 4.0 88 4.0 89 3.5 90 3.0 91 2.0 92 2.0 93 2.0 94 1.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.324350973701623 2 0.27530910647793766 3 0.26739494271961806 4 0.27524423628319733 5 0.27900670757813617 6 0.21167144543767918 7 0.200643512331824 8 0.20200578642137085 9 0.21387703205885022 10-11 0.21290397913774536 12-13 0.20761705826640892 14-15 0.21001725547180095 16-17 0.19483762990256498 18-19 0.19970289450808928 20-21 0.18925879315489705 22-23 0.19804870454221105 24-25 0.19785409395799003 26-27 0.19821088002906184 28-29 0.21001725547180095 30-31 0.20719540200059683 32-33 0.2133905055982978 34-35 0.2063520894689726 36-37 0.1905886321470737 38-39 0.1801120956965113 40-41 0.17699832634897572 42-43 0.19071837253655435 44-45 0.1945457140262335 46-47 0.19357266110512863 48-49 0.21238501757982275 50-51 0.20758462316903875 52-53 0.22899178743334586 54-55 0.19551876694733836 56-57 0.17621988401209182 58-59 0.1789768672885556 60-61 0.16652178989841326 62-63 0.17313854976192639 64-65 0.17320341995666672 66-67 0.1714843597960481 68-69 0.1742413430725119 70-71 0.17625231910946199 72-73 0.17404673248829092 74-75 0.1887722666943446 76-77 0.18040401157284275 78-79 0.1839718722835606 80-81 0.17797137927008055 82-83 0.15912658769801627 84-85 0.1578940539979501 86-87 0.16311610467454624 88-89 0.16820841496166172 90-91 0.17388455700144012 92-93 0.1706410472644239 94-95 0.17025182609598194 96-97 0.17352777093036834 98-99 0.18367995640722914 100-101 0.16535412639308741 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1541540.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.3420784504291 #Duplication Level Percentage of deduplicated Percentage of total 1 75.27875448221334 40.90803981723029 2 13.691229537060654 14.880197391715644 3 4.699734117174844 7.661799602750206 4 2.1096633731444188 4.5857397010964345 5 1.1538046993430817 3.1350072744087756 6 0.70207214823994 2.2891235854509664 7 0.46602704715174825 1.772741484943949 8 0.32275503002528366 1.4031343329523651 9 0.22618145864226066 1.1062053512613192 >10 1.2056120909979802 11.828981361870062 >50 0.09555611657530451 3.6035634634334626 >100 0.04586300752970999 4.329771684304885 >500 0.001910881322716102 0.6597165223990522 >1k 5.971504133487818E-4 0.7256557242443484 >5k 2.3886016533951275E-4 1.1103227019383164 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9584 0.6217159463912711 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7524 0.4880833452262024 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4989 0.32363740155947945 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2076 0.1346705242809139 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1916 0.12429129312246194 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.2435097370162304E-5 0.0 22-23 0.0 0.0 0.0 1.2974038948064922E-4 0.0 24-25 0.0 0.0 0.0 3.8922116844194765E-4 0.0 26-27 0.0 0.0 0.0 6.162668500330838E-4 0.0 28-29 0.0 0.0 0.0 0.001362274089546817 0.0 30-31 0.0 0.0 0.0 0.0035354256133476912 0.0 32-33 0.0 0.0 0.0 0.006195103597701 0.0 34-35 0.0 0.0 0.0 0.009308872945236581 0.0 36-37 0.0 0.0 0.0 0.013330825019136707 0.0 38-39 0.0 0.0 0.0 0.019201577643136084 0.0 40-41 0.0 0.0 0.0 0.027115741401455687 0.0 42-43 0.0 0.0 0.0 0.03804636921520038 0.0 44-45 0.0 0.0 0.0 0.04845803547102248 0.0 46-47 0.0 0.0 0.0 0.06668656019305369 0.0 48-49 0.0 0.0 0.0 0.09033174617590202 0.0 50-51 0.0 0.0 0.0 0.10252734278708305 0.0 52-53 0.0 0.0 0.0 0.12348041568820789 0.0 54-55 0.0 0.0 0.0 0.15516950581885647 0.0 56-57 0.0 0.0 0.0 0.20690348612426535 0.0 58-59 0.0 0.0 0.0 0.2724223828119932 0.0 60-61 0.0 0.0 0.0 0.34569326777118986 0.0 62-63 0.0 0.0 0.0 0.42321315048587776 0.0 64-65 0.0 0.0 0.0 0.5079011897193715 0.0 66-67 0.0 0.0 0.0 0.6187319174332162 0.0 68-69 0.0 0.0 0.0 0.7473370785059097 0.0 70-71 0.0 0.0 0.0 0.8371498631238892 0.0 72-73 0.0 0.0 0.0 0.9578408604382631 0.0 74-75 0.0 0.0 0.0 1.0786615981421175 0.0 76-77 0.0 0.0 0.0 1.222835605952489 0.0 78-79 0.0 0.0 0.0 1.3863733668928475 0.0 80-81 0.0 0.0 0.0 1.6126081710497293 0.0 82-83 0.0 0.0 0.0 1.8528873723678918 0.0 84-85 0.0 0.0 0.0 2.164880573971483 0.0 86-87 0.0 0.0 0.0 2.541516924633808 0.0 88-89 0.0 0.0 0.0 2.990970068892147 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5590 0.0 39.04284 1 GTATCAA 9185 0.0 37.169422 1 ATCAACG 11260 0.0 30.094967 3 TCAACGC 11325 0.0 29.925156 4 TATCAAC 11370 0.0 29.76298 2 CAACGCA 11535 0.0 29.213629 5 AACGCAG 11735 0.0 28.861788 6 ACGCAGA 13210 0.0 25.603184 7 CGCAGAG 13210 0.0 25.387426 8 GTACATG 7210 0.0 25.126398 1 GCAGAGT 13805 0.0 23.949123 9 GAGTACT 7565 0.0 23.703018 12-13 TACATGG 7550 0.0 23.481117 2 GTACTTT 8020 0.0 22.20876 14-15 ACATGGG 7465 0.0 22.15604 3 AGTACTT 7810 0.0 22.077566 12-13 AGAGTAC 12130 0.0 20.87224 10-11 CGGTATA 115 0.003710788 20.664696 2 ACAACCG 400 0.0 20.19974 5 CATGGGG 2915 0.0 20.05634 4 >>END_MODULE