FastQCFastQC Report
Thu 26 May 2016
SRR937818_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937818_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541540
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87450.5672898530041387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73870.47919612854677784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50760.32928110850188774No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19230.12474538448564423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67100.035.1247521
GTATCAA101450.031.615921
CGTCGAT350.00839718927.14055844-45
ATCAACG124200.025.7415583
TCAACGC125050.025.5665864
TATCAAC125550.025.4647672
GTACATG77000.025.3015541
CAACGCA126900.025.1946815
AACGCAG128950.024.826966
TACATGG80400.024.048042
ACATGGG80900.023.4883673
GAGTACT76100.022.75344512-13
ACGCAGA144400.022.0097147
CGCAGAG144950.021.8606518
GTACTTT80250.021.60253114-15
CGCATCG550.002879866921.5890874-75
AGTACTT78600.021.33469812-13
GCAGAGT152250.020.438059
CATGGGA51900.020.4116864
TCATACG1456.1427907E-419.6573352
Produced by FastQC (version 0.11.5)