##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937818_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1541540 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26564215005774 33.0 31.0 34.0 31.0 34.0 2 32.47891005098797 34.0 31.0 34.0 31.0 34.0 3 32.55482309897894 34.0 31.0 34.0 31.0 34.0 4 36.16451016515951 37.0 35.0 37.0 35.0 37.0 5 36.081456854833476 37.0 35.0 37.0 35.0 37.0 6 36.12164134566732 37.0 35.0 37.0 35.0 37.0 7 36.069034861242656 37.0 35.0 37.0 35.0 37.0 8 36.051215018747484 37.0 35.0 37.0 35.0 37.0 9 37.73380256107529 39.0 37.0 39.0 35.0 39.0 10-11 37.66833718229822 39.0 37.0 39.0 35.0 39.0 12-13 37.57144738378504 39.0 37.0 39.0 35.0 39.0 14-15 38.79770359510619 40.0 38.0 41.0 35.0 41.0 16-17 38.76563533868729 40.0 38.0 41.0 35.0 41.0 18-19 38.759941357343955 40.0 38.0 41.0 35.0 41.0 20-21 38.663883519078325 40.0 38.0 41.0 34.0 41.0 22-23 38.50483250515718 40.0 38.0 41.0 34.0 41.0 24-25 38.581090338233196 40.0 38.0 41.0 34.0 41.0 26-27 38.46231884998119 40.0 38.0 41.0 34.0 41.0 28-29 38.31311188811189 40.0 38.0 41.0 34.0 41.0 30-31 38.350152444957644 40.0 38.0 41.0 34.0 41.0 32-33 38.27363934766532 40.0 38.0 41.0 34.0 41.0 34-35 38.15079076767388 40.0 38.0 41.0 34.0 41.0 36-37 37.992272013700585 40.0 37.0 41.0 33.5 41.0 38-39 37.845565149136576 40.0 37.0 41.0 33.0 41.0 40-41 37.66136363636363 40.0 37.0 41.0 33.0 41.0 42-43 37.433857700740816 40.0 37.0 41.0 33.0 41.0 44-45 37.09863286064585 40.0 36.5 41.0 31.5 41.0 46-47 36.70798065570793 40.0 36.0 41.0 31.0 41.0 48-49 36.35200838122916 39.5 36.0 41.0 30.0 41.0 50-51 36.086509269950824 39.0 36.0 41.0 29.5 41.0 52-53 35.840479650219905 39.0 35.0 40.5 29.0 41.0 54-55 35.61364674286752 39.0 35.0 40.0 28.0 41.0 56-57 35.360767479273974 38.5 35.0 40.0 28.0 41.0 58-59 35.101432333899865 38.0 34.5 40.0 27.0 41.0 60-61 34.75205216861061 38.0 34.0 40.0 26.0 41.0 62-63 34.39908078934053 37.5 34.0 40.0 26.0 41.0 64-65 34.01401715167949 37.0 33.5 40.0 25.5 41.0 66-67 33.58085453507532 36.5 33.0 39.0 24.5 40.5 68-69 33.13878426768038 36.0 33.0 39.0 23.0 40.0 70-71 32.71918244093568 35.5 32.0 38.5 22.5 40.0 72-73 32.21763463808918 35.0 31.5 37.5 21.5 40.0 74-75 31.71795055593757 35.0 31.0 37.0 20.0 39.0 76-77 30.48536495971561 33.5 29.5 35.5 19.5 38.5 78-79 31.082851564020395 34.0 31.0 36.0 19.5 38.5 80-81 30.879717036210543 34.0 31.0 36.0 18.0 37.5 82-83 30.522544338778104 34.0 31.0 35.0 16.5 37.0 84-85 30.138341204250295 34.0 30.0 35.0 11.0 36.5 86-87 29.725196556690065 34.0 30.0 35.0 7.0 36.0 88-89 29.35201097603695 34.0 29.5 35.0 2.0 36.0 90-91 28.834190160488863 34.0 29.0 35.0 2.0 35.0 92-93 28.37842514628229 34.0 29.0 35.0 2.0 35.0 94-95 27.909526836799564 33.0 27.5 35.0 2.0 35.0 96-97 27.359127560750938 33.0 27.0 35.0 2.0 35.0 98-99 26.478572401624348 33.0 25.5 34.5 2.0 35.0 100-101 23.826536450562426 30.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3788913834086358 1101 2 0.34896291540392355 1101 3 0.293499176589215 1101 4 0.2167776128451635 1101 5 0.2543112300649639 1101 6 0.2844028896330997 1101 7 0.2483128596620503 1101 8 0.1630406003689373 1101 9 0.3838326938274008 1101 10-11 0.37789502278828735 1101 12-13 0.37666496054638543 1101 14-15 0.34082627359607187 1101 16-17 0.35770339234704096 1101 18-19 0.41975198045470563 1101 20-21 0.405013194503141 1101 22-23 0.54593664542071 1101 24-25 0.4741146721836742 1101 26-27 0.3512754944035166 1101 28-29 0.3467740593636748 1101 30-31 0.3774959440466006 1101 32-33 0.3482592418172814 1101 34-35 0.3662433891739525 1101 36-37 0.343922457526709 1101 38-39 0.3587757895199317 1101 40-41 0.4015279708535431 1101 42-43 0.409712977866306 1101 44-45 0.4166863268852907 1101 46-47 0.15977629186336628 1101 48-49 0.012884729779294446 1101 50-51 0.04940046974130041 1101 52-53 0.04845670091395249 1101 54-55 0.10124803206262811 1101 56-57 -0.04369782503473374 1101 58-59 0.01535316750075566 1101 60-61 -0.001958455376957602 1101 62-63 -0.022548476503743586 1101 64-65 0.07548555629136189 1101 66-67 -0.06359270046647225 1101 68-69 0.00514812729925751 1101 70-71 0.0886576850760008 1101 72-73 -0.0035771169653315837 1101 74-75 -0.009501224805365638 1101 76-77 -0.021562249073230788 1101 78-79 0.039983915682412885 1101 80-81 0.009145029856796327 1101 82-83 0.060576125756629295 1101 84-85 0.0013656894293454513 1101 86-87 -0.17300003804473008 1101 88-89 0.08420822845596021 1101 90-91 0.05246178226193976 1101 92-93 0.004108879635257523 1101 94-95 0.06996666001614571 1101 96-97 0.416063008677785 1101 98-99 0.5292388658284182 1101 100-101 0.9852831686008585 1102 1 0.3043053615069198 1102 2 0.2618281237178408 1102 3 0.27317311276461 1102 4 0.16673609332820405 1102 5 0.15765073452917733 1102 6 0.1741899230253665 1102 7 0.2405199871095789 1102 8 0.26060604055231806 1102 9 0.31979666295412557 1102 10-11 0.3451139384403703 1102 12-13 0.3381169057044531 1102 14-15 0.4075114861106286 1102 16-17 0.29105462242358016 1102 18-19 0.3380724499242973 1102 20-21 0.41870016933688703 1102 22-23 0.49131309599579254 1102 24-25 0.336403962373808 1102 26-27 0.28125759302384523 1102 28-29 0.38379889735410444 1102 30-31 0.4479965011175082 1102 32-33 0.4227612510280778 1102 34-35 0.32852767198514954 1102 36-37 0.310357301094804 1102 38-39 0.30250536880273415 1102 40-41 0.2538132134123643 1102 42-43 0.26972766617783606 1102 44-45 0.403556422957422 1102 46-47 0.48524396019127636 1102 48-49 0.32294024213847905 1102 50-51 0.3906908991101332 1102 52-53 0.21529853311390212 1102 54-55 0.31505309758279054 1102 56-57 0.24784930205114364 1102 58-59 0.2650000777393089 1102 60-61 0.16813706037611098 1102 62-63 0.15874548584910997 1102 64-65 0.24495195051952834 1102 66-67 0.28706542212807307 1102 68-69 0.2320241209510172 1102 70-71 0.2854272301973566 1102 72-73 0.3385200567865496 1102 74-75 0.30184507856668574 1102 76-77 0.30375825795170286 1102 78-79 0.5212038801022061 1102 80-81 0.5335729715875956 1102 82-83 0.3925827354968128 1102 84-85 0.49755396131333285 1102 86-87 0.6468896883437409 1102 88-89 0.6247184803688484 1102 90-91 0.6291269791701595 1102 92-93 0.6391304878662751 1102 94-95 0.813178253767493 1102 96-97 1.1375886179436598 1102 98-99 1.28757177028902 1102 100-101 1.5541296214988094 1103 1 0.1847312181254992 1103 2 0.19978095973397814 1103 3 0.18034581724482734 1103 4 0.08197173156809612 1103 5 0.14776737273273 1103 6 0.20082776322012563 1103 7 0.17327679100478832 1103 8 0.1312175735634753 1103 9 0.2386949588009344 1103 10-11 0.21197560771111768 1103 12-13 0.1304136972454586 1103 14-15 0.32222570434397113 1103 16-17 0.3079453604054905 1103 18-19 0.22072206294708252 1103 20-21 0.29153727554669473 1103 22-23 0.32439510609717104 1103 24-25 0.21129586310415505 1103 26-27 0.13602849524396987 1103 28-29 0.027565475603438472 1103 30-31 0.04377245402619678 1103 32-33 0.1080524831780707 1103 34-35 0.20779078015709018 1103 36-37 0.17113452575960508 1103 38-39 0.12310600104719782 1103 40-41 0.04037075129253509 1103 42-43 0.08843338181316795 1103 44-45 0.09645093987177233 1103 46-47 0.22730093486516267 1103 48-49 0.19183127080749074 1103 50-51 0.05653698028450549 1103 52-53 -0.1721110813623099 1103 54-55 0.004165048456663101 1103 56-57 -0.1423400079303221 1103 58-59 -0.10506641693748264 1103 60-61 -0.19706405876926425 1103 62-63 -0.16798501086249473 1103 64-65 -0.07194242105126847 1103 66-67 -0.12387349724259167 1103 68-69 -0.11066873177962577 1103 70-71 0.014744842202730979 1103 72-73 0.022279731216983123 1103 74-75 -0.019200668317512992 1103 76-77 0.03153341147083566 1103 78-79 0.03885585495776667 1103 80-81 0.12441996115919451 1103 82-83 0.23683803145263127 1103 84-85 0.08574479740213903 1103 86-87 0.2057701796049578 1103 88-89 0.13232721551655402 1103 90-91 0.28137798749108 1103 92-93 0.3342923642515814 1103 94-95 0.27069433239836016 1103 96-97 0.40009361412839084 1103 98-99 0.6595822350250415 1103 100-101 0.8744722689316795 1104 1 0.2580598747330711 1104 2 0.23478134683448815 1104 3 0.16656769852966846 1104 4 0.060995611828154495 1104 5 0.11202233426794095 1104 6 0.12988346846110943 1104 7 0.15272559533700303 1104 8 0.13670464942985916 1104 9 0.24265638586026483 1104 10-11 0.22375616959193678 1104 12-13 0.2655085250507483 1104 14-15 0.3958408448791886 1104 16-17 0.3351040071966551 1104 18-19 0.30932080046751764 1104 20-21 0.21527153075053462 1104 22-23 0.25888957351482844 1104 24-25 0.19444044307645925 1104 26-27 0.21662030987069159 1104 28-29 0.042363352992452974 1104 30-31 0.3205388291165434 1104 32-33 0.16827911722979394 1104 34-35 0.20844826003229855 1104 36-37 0.24948204391343864 1104 38-39 0.2306299643417944 1104 40-41 0.1726020695559356 1104 42-43 0.07199904440217608 1104 44-45 0.20848299888598376 1104 46-47 0.03255997602606442 1104 48-49 0.06665966117265043 1104 50-51 0.07043817332237268 1104 52-53 0.15694621956016874 1104 54-55 0.08444275022615955 1104 56-57 0.1595404731617407 1104 58-59 0.11524784957797607 1104 60-61 0.08288643964480968 1104 62-63 0.17868669303575757 1104 64-65 0.09427602656338507 1104 66-67 0.005285838646592822 1104 68-69 0.1918353948300151 1104 70-71 0.21596899585622964 1104 72-73 0.22312949071232424 1104 74-75 0.013164480343856866 1104 76-77 0.15399516978182604 1104 78-79 0.0679336042431089 1104 80-81 0.16526054253097655 1104 82-83 0.33624086242799933 1104 84-85 0.2887273034140172 1104 86-87 0.2942417058506699 1104 88-89 0.2155972633338088 1104 90-91 0.19575253648943303 1104 92-93 0.27545825780071453 1104 94-95 0.5549581942903536 1104 96-97 0.8544048204813315 1104 98-99 0.9677677264978968 1104 100-101 1.1374569793479914 1105 1 0.13741849311408316 1105 2 0.14074905605036747 1105 3 0.22534102054127203 1105 4 0.1452180083703425 1105 5 0.11454208814602396 1105 6 0.1709466011080636 1105 7 0.07009751465211878 1105 8 0.16892509146703816 1105 9 0.16608714507115252 1105 10-11 0.1313340530363618 1105 12-13 0.21406867915014516 1105 14-15 0.14701828969048591 1105 16-17 0.1527867505881133 1105 18-19 0.2070909878600844 1105 20-21 0.20884841935693998 1105 22-23 0.05864120943928697 1105 24-25 0.2881035949418731 1105 26-27 0.12148232947979665 1105 28-29 0.2296716001619714 1105 30-31 0.18119948572219613 1105 32-33 0.3241268680731082 1105 34-35 0.1667653947983112 1105 36-37 0.024920912561533726 1105 38-39 0.08896415078222475 1105 40-41 0.10635286011929423 1105 42-43 0.10901436704309475 1105 44-45 -0.023192888664652855 1105 46-47 -0.04331102538768761 1105 48-49 0.15118289572769328 1105 50-51 0.18480278020119556 1105 52-53 -0.0346996846778822 1105 54-55 -0.008424970859948644 1105 56-57 -0.046713032166358914 1105 58-59 -0.06641371833448062 1105 60-61 -0.09478305808132603 1105 62-63 0.022980725452150352 1105 64-65 0.0944905071090929 1105 66-67 0.08251485048479168 1105 68-69 -0.16470805176872005 1105 70-71 -0.07777106125903543 1105 72-73 -0.022226361717528675 1105 74-75 -0.1806194777674328 1105 76-77 -0.27981512892708693 1105 78-79 -0.21642818895242755 1105 80-81 -0.1897594013335322 1105 82-83 -0.13100924575435258 1105 84-85 -0.10082809066179976 1105 86-87 0.04086151757254797 1105 88-89 -0.1249238898492635 1105 90-91 0.033047688726657753 1105 92-93 0.1180990942685618 1105 94-95 0.25991413942101715 1105 96-97 0.2691798922522395 1105 98-99 0.410687010842576 1105 100-101 0.5288249381526526 1106 1 0.13307856685630526 1106 2 0.13980582508005313 1106 3 0.1045649045678374 1106 4 0.1049211246494437 1106 5 0.07337821204827577 1106 6 0.11972809562562503 1106 7 0.06660472327708078 1106 8 0.09483749013176634 1106 9 0.10027586360322971 1106 10-11 -0.016281061920800255 1106 12-13 -0.08201116783367723 1106 14-15 0.1763992252195905 1106 16-17 0.26104839009374814 1106 18-19 0.18082572861695212 1106 20-21 0.16053690917323138 1106 22-23 0.17355918735066211 1106 24-25 0.15093033050459326 1106 26-27 0.25893661291858194 1106 28-29 0.31518665746148145 1106 30-31 0.3312953690509133 1106 32-33 0.24909124869304833 1106 34-35 0.14942395156982258 1106 36-37 0.073421423537404 1106 38-39 0.10113644007471834 1106 40-41 0.19529617193797577 1106 42-43 -0.12397112918503694 1106 44-45 0.1676807134209497 1106 46-47 0.17403013416231516 1106 48-49 0.14001128229921278 1106 50-51 0.21365997432310024 1106 52-53 0.2658833809929817 1106 54-55 0.21623438446651022 1106 56-57 -0.039588269658445085 1106 58-59 -0.03963872430946225 1106 60-61 -0.0123604508916344 1106 62-63 0.12180154490000916 1106 64-65 0.08854566980934209 1106 66-67 0.0392889863823882 1106 68-69 0.15921253385715062 1106 70-71 0.18186035528239586 1106 72-73 0.27473022909114775 1106 74-75 0.1554533623577541 1106 76-77 0.12257855014886232 1106 78-79 0.27608500387022517 1106 80-81 0.09620553458704251 1106 82-83 0.17860383806483782 1106 84-85 0.13275738556700745 1106 86-87 0.3062854715258574 1106 88-89 0.06525718212918719 1106 90-91 0.36907638138725574 1106 92-93 0.39829276866381136 1106 94-95 0.6281123271410003 1106 96-97 0.47181905959914516 1106 98-99 0.6962913204861536 1106 100-101 0.6400815132880915 1107 1 0.12536563684322743 1107 2 0.17975902891449635 1107 3 0.12731166868192645 1107 4 0.0020997212686566513 1107 5 -0.023681644960014125 1107 6 0.01751067969383513 1107 7 0.11663330206992129 1107 8 0.0957537606996226 1107 9 0.014784887144848824 1107 10-11 0.12759502496079023 1107 12-13 0.17848002727433254 1107 14-15 0.28297600030732184 1107 16-17 0.24682304515466313 1107 18-19 0.2647797267926464 1107 20-21 0.21753680988057766 1107 22-23 0.2567924134513504 1107 24-25 0.22128072039546964 1107 26-27 0.27317893535836646 1107 28-29 0.22555960170480205 1107 30-31 0.18264474595091684 1107 32-33 0.2752561496138952 1107 34-35 0.3359786456172529 1107 36-37 0.11437012627629173 1107 38-39 0.2568576777448044 1107 40-41 0.2583699021366854 1107 42-43 0.4630442210479373 1107 44-45 0.4848898798308028 1107 46-47 0.41357700981344436 1107 48-49 0.16912640981824723 1107 50-51 0.24878539432332047 1107 52-53 0.18614494768703338 1107 54-55 0.2355897641991831 1107 56-57 0.07981011974265328 1107 58-59 -0.04774882494488253 1107 60-61 0.16287102189626523 1107 62-63 0.1683031765407179 1107 64-65 0.18121066925114349 1107 66-67 0.12688877421508238 1107 68-69 0.20542889203920112 1107 70-71 0.23549176551077977 1107 72-73 0.24410564741933172 1107 74-75 0.10988214920214645 1107 76-77 0.08370727613148432 1107 78-79 0.17890836223594064 1107 80-81 0.2697073601828386 1107 82-83 0.2177189707285727 1107 84-85 0.3661049011155697 1107 86-87 0.3797908469627025 1107 88-89 0.525851888544473 1107 90-91 0.4969043571186944 1107 92-93 0.32988373025401785 1107 94-95 0.7296596462650236 1107 96-97 0.9010797306014169 1107 98-99 0.7984688650147973 1107 100-101 0.8763651075175964 1108 1 0.20200962696144842 1108 2 0.26523744921436077 1108 3 0.10635882421251353 1108 4 0.09566881436179386 1108 5 0.09449466430945819 1108 6 0.08788253453052164 1108 7 0.16778913803056383 1108 8 0.1189014946551552 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0.16956138815503863 2316 68-69 0.4143369754455293 2316 70-71 0.5208122351809763 2316 72-73 0.5232370618091267 2316 74-75 0.6950543679056302 2316 76-77 0.35452430699572446 2316 78-79 0.4384261041281228 2316 80-81 0.39858925357935604 2316 82-83 0.30233537259508125 2316 84-85 0.23326915145387872 2316 86-87 0.3498344074915565 2316 88-89 0.43590451714050005 2316 90-91 0.6746464282771676 2316 92-93 0.7693335014145823 2316 94-95 0.7544387364157288 2316 96-97 0.6538839210029899 2316 98-99 1.0646623747843442 2316 100-101 1.3778704862138014 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 10.0 4 14.0 5 36.0 6 59.0 7 159.0 8 388.0 9 722.0 10 1257.0 11 2016.0 12 3098.0 13 4593.0 14 5990.0 15 8612.0 16 12153.0 17 17872.0 18 16021.0 19 9064.0 20 7409.0 21 7379.0 22 7668.0 23 8510.0 24 9625.0 25 11369.0 26 13781.0 27 17266.0 28 21418.0 29 26672.0 30 33996.0 31 42846.0 32 55300.0 33 73723.0 34 101342.0 35 147887.0 36 230468.0 37 347996.0 38 271555.0 39 23264.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.9163000625498 20.086272005979865 15.348189559633838 21.6492383718365 2 15.388637336689284 21.273791143921013 40.04975543936583 23.28781608002387 3 19.34254057630681 25.447798954292463 30.32337792078052 24.886282548620212 4 12.922670136791869 17.54882620549983 37.15021157427739 32.37829208343091 5 13.371988149201705 38.277546422764125 33.86522636815597 14.485239059878197 6 31.113886113886114 37.31281705307679 17.04042710536217 14.532869727674921 7 28.025156661520295 34.02020057864213 20.33304357979683 17.62159918004074 8 26.441545467519497 35.50994460085369 19.377440741077105 18.671069190549712 9 26.70426975621781 17.17574633159049 20.486202109578734 35.63378180261297 10-11 24.9704921605966 27.616515827197425 26.703499975187146 20.709492037018837 12-13 25.84369887238677 25.024327175206263 27.825195947287227 21.306778005119735 14-15 22.67826329514641 26.28436498566369 26.555392659288763 24.48197905990114 16-17 21.47647806738716 29.811033122721437 27.159009821347485 21.553478988543922 18-19 21.332466235063638 27.777157907028037 30.338006149694465 20.552369708213863 20-21 22.20756516211062 26.794569067296344 30.575982459099343 20.4218833114937 22-23 21.93995493425182 26.73572380087128 30.42067743932877 20.903643825548134 24-25 21.818019642694967 27.05242809138913 30.490678153015814 20.638874112900087 26-27 21.535640171180248 27.295750399115686 30.45345120733711 20.715158222366956 28-29 21.22716539519435 27.251576366184025 30.708164668638748 20.813093569982875 30-31 21.291922363350935 27.091382643330697 30.68944042970017 20.9272545636182 32-33 21.330812045097762 27.272111005877242 30.41980746526201 20.97726948376299 34-35 21.77011300387924 27.117784812590006 30.271579070280367 20.840523113250388 36-37 21.81454908727636 27.06423446683187 30.112582222971835 21.008634222919937 38-39 21.5661032364075 27.302673723248738 30.23383442331513 20.897388617028625 40-41 21.56684795864882 27.24037448557535 30.27992355636745 20.91285399940838 42-43 21.55561056014606 27.340489481020875 29.894364152520737 21.20953580631233 44-45 21.67880820478223 27.63223789197815 29.52787472267992 21.1610791805597 46-47 21.834042580795828 27.87751209829132 28.91063481972573 21.377810501187124 48-49 22.02862072991943 27.936349364920794 28.741647962427187 21.29338194273259 50-51 22.076040842274608 28.162259818103973 28.438314931821424 21.323384407799992 52-53 22.245968317396887 28.425957159723392 28.08321548581289 21.244859037066828 54-55 21.771442842871412 28.419340399859884 28.27873425276023 21.530482504508477 56-57 21.88029502964568 28.774050624699974 28.14318798085032 21.202466364804025 58-59 21.942797462277984 28.178574672081165 28.370493143220415 21.508134722420436 60-61 21.9138653554238 28.21733461343851 28.740220818142898 21.128579212994797 62-63 21.846108437017527 28.30140638582197 28.71154819206767 21.14093698509283 64-65 21.810429829910348 28.133619627126123 28.606815262659417 21.44913528030411 66-67 21.606121151575696 28.624135604655088 28.28943783489238 21.480305408876838 68-69 21.660672652896004 28.41447956521344 28.44425502323005 21.480592758660507 70-71 21.7546090273363 28.5460643252851 28.30370927773525 21.395617369643343 72-73 21.887787537138188 28.37552058331279 28.25181312194299 21.48487875760603 74-75 21.785876461201138 28.80687494323858 27.81812343500655 21.58912516055373 76-77 21.870856416310964 28.568833763638956 28.04429336896869 21.516016451081384 78-79 21.94257041659639 28.546583286843024 27.90767673884557 21.603169557715013 80-81 21.687208895001103 29.003431633301762 27.804597999403196 21.50476147229394 82-83 21.812582204699634 28.80319688096516 27.74757095755602 21.63664995677918 84-85 21.672611496783563 28.591589360089344 27.769422111913737 21.96637703121336 86-87 21.671283262192354 28.512948090870164 27.995608287816083 21.8201603591214 88-89 21.74805713766753 28.391316475732058 27.90809839511138 21.952527991489028 90-91 21.55229097930043 28.644694086814866 28.006686300836176 21.79632863304853 92-93 21.579488363810572 28.54796820381115 27.966847993730802 21.905695438647477 94-95 21.364447241070618 28.541588281848025 27.99781387443725 22.09615060264411 96-97 21.561068801328542 28.60749639970419 27.831681305707278 21.999753493259988 98-99 21.44789379279475 28.86280704371998 27.4692753557377 22.220023807747573 100-101 21.34500884519944 28.689395460707885 27.7901145934274 22.17548110066528 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 523.0 1 361.5 2 256.0 3 328.5 4 307.0 5 314.0 6 578.0 7 1205.0 8 1893.5 9 2350.0 10 2372.0 11 2336.5 12 2507.5 13 2694.5 14 2995.5 15 3219.0 16 3349.5 17 3482.0 18 3520.0 19 3723.0 20 4022.5 21 4440.0 22 5127.0 23 6042.0 24 7062.5 25 8478.5 26 10452.0 27 12760.0 28 14695.0 29 17149.0 30 20136.5 31 22822.0 32 25960.0 33 29827.0 34 34446.5 35 37872.0 36 41919.5 37 45955.0 38 47921.0 39 48626.0 40 48507.5 41 50100.5 42 52831.0 43 55049.5 44 56870.5 45 57776.5 46 56791.0 47 56961.5 48 61541.0 49 61244.0 50 57009.0 51 54239.5 52 50769.0 53 47753.5 54 44500.5 55 40834.0 56 36126.5 57 31145.0 58 27338.0 59 24269.0 60 20916.0 61 16947.0 62 13063.0 63 9741.0 64 6908.0 65 5026.0 66 3630.0 67 2755.0 68 2005.5 69 1289.0 70 930.0 71 731.5 72 616.5 73 478.5 74 351.0 75 273.5 76 179.5 77 100.0 78 52.0 79 35.5 80 26.5 81 18.5 82 15.5 83 11.5 84 7.5 85 3.5 86 1.0 87 0.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023872231664439456 2 0.0 3 0.0 4 6.487019474032461E-5 5 0.0245209336118427 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.2435097370162304E-5 12-13 0.003502990515977529 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.406178237347068E-4 24-25 0.0 26-27 4.540913631822723E-4 28-29 0.0012974038948064923 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 3.2435097370162304E-5 40-41 7.784423368838953E-4 42-43 0.016509464561412612 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.2974038948064922E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0024326323027621726 84-85 2.9191587633146075E-4 86-87 0.0 88-89 0.0 90-91 0.011644199955888267 92-93 0.002238021718541199 94-95 0.0 96-97 0.0 98-99 0.0016217548685081152 100-101 0.020012455077390145 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1541540.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.72858440343605 #Duplication Level Percentage of deduplicated Percentage of total 1 73.38286977520998 37.959919729333066 2 14.360603295157764 14.85707359275661 3 5.12510042596036 7.953425698811298 4 2.346456257620694 4.85515442285211 5 1.31533326336977 3.4020163866435067 6 0.7874387709517493 2.4439855755429316 7 0.528130269040077 1.9123601838634312 8 0.3709611333212624 1.5351435436322574 9 0.2662972486239858 1.239766173166378 >10 1.3565103048036935 12.61176580688386 >50 0.10106011475857458 3.6006266853322395 >100 0.05634028416027742 5.136986922724289 >500 0.0018905589272675044 0.6393246390684894 >1k 6.301863090891682E-4 0.46974648424316395 >5k 3.781117854535009E-4 1.3827041551462902 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8745 0.5672898530041387 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7387 0.47919612854677784 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5076 0.32928110850188774 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1923 0.12474538448564423 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.2435097370162304E-5 0.0 22-23 0.0 0.0 0.0 1.2974038948064922E-4 0.0 24-25 0.0 0.0 0.0 3.8922116844194765E-4 0.0 26-27 0.0 0.0 0.0 6.162668500330838E-4 0.0 28-29 0.0 0.0 0.0 0.0012649687974363299 0.0 30-31 0.0 0.0 0.0 0.0035678607107178534 0.0 32-33 0.0 0.0 0.0 0.006259973792441325 0.0 34-35 0.0 0.0 0.0 0.009438613334717232 0.0 36-37 0.0 0.0 0.0 0.013428130311247195 0.0 38-39 0.0 0.0 0.0 0.019428623324727223 0.0 40-41 0.0 0.0 0.0 0.027277916888306497 0.0 42-43 0.0 0.0 0.0 0.03801393411783022 0.0 44-45 0.0 0.0 0.0 0.048360730178912 0.0 46-47 0.0 0.0 0.0 0.06671899529042385 0.0 48-49 0.0 0.0 0.0 0.09033174617590202 0.0 50-51 0.0 0.0 0.0 0.1023002971054919 0.0 52-53 0.0 0.0 0.0 0.12341554549346757 0.0 54-55 0.0 0.0 0.0 0.15565603227940888 0.0 56-57 0.0 0.0 0.0 0.20794140924011054 0.0 58-59 0.0 0.0 0.0 0.27417387806998195 0.0 60-61 0.0 0.0 0.0 0.3479961596844714 0.0 62-63 0.0 0.0 0.0 0.4255160423991593 0.0 64-65 0.0 0.0 0.0 0.5107230431905756 0.0 66-67 0.0 0.0 0.0 0.6216510761965308 0.0 68-69 0.0 0.0 0.0 0.7510346796061081 0.0 70-71 0.0 0.0 0.0 0.8420475628267836 0.0 72-73 0.0 0.0 0.0 0.9632899567964504 0.0 74-75 0.0 0.0 0.0 1.0845972209608572 0.0 76-77 0.0 0.0 0.0 1.229419930718632 0.0 78-79 0.0 0.0 0.0 1.3944172710406475 0.0 80-81 0.0 0.0 0.0 1.6238307147398057 0.0 82-83 0.0 0.0 0.0 1.8664128079712494 0.0 84-85 0.0 0.0 0.0 2.181844129896078 0.0 86-87 0.0 0.0 0.0 2.5644485384745126 0.0 88-89 0.0 0.0 0.0 3.0214266253227295 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6710 0.0 35.124752 1 GTATCAA 10145 0.0 31.61592 1 CGTCGAT 35 0.008397189 27.140558 44-45 ATCAACG 12420 0.0 25.741558 3 TCAACGC 12505 0.0 25.566586 4 TATCAAC 12555 0.0 25.464767 2 GTACATG 7700 0.0 25.301554 1 CAACGCA 12690 0.0 25.194681 5 AACGCAG 12895 0.0 24.82696 6 TACATGG 8040 0.0 24.04804 2 ACATGGG 8090 0.0 23.488367 3 GAGTACT 7610 0.0 22.753445 12-13 ACGCAGA 14440 0.0 22.009714 7 CGCAGAG 14495 0.0 21.860651 8 GTACTTT 8025 0.0 21.602531 14-15 CGCATCG 55 0.0028798669 21.58908 74-75 AGTACTT 7860 0.0 21.334698 12-13 GCAGAGT 15225 0.0 20.43805 9 CATGGGA 5190 0.0 20.411686 4 TCATACG 145 6.1427907E-4 19.657335 2 >>END_MODULE