FastQCFastQC Report
Thu 26 May 2016
SRR937817_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937817_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407450
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93340.6631851930796832No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72380.5142633841344275No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46760.33223205087214464No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20310.14430352765639987No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17740.12604355394507796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48450.045.5317271
GTATCAA83400.038.935391
ATCAACG102750.031.2579653
TATCAAC102700.031.234712
TCAACGC103100.031.0619034
CAACGCA104800.030.5126955
AACGCAG107300.029.7954066
ACGCAGA118900.026.7686797
CGCAGAG118400.026.7613548
GTACATG66050.025.6251891
TACATGG67300.024.7854162
GCAGAGT123650.024.7790189
GAGTACT72250.023.96248212-13
ACATGGG68000.023.8254623
GTAGCGC1000.00165156323.7555923
GTACTTT76650.022.30809414-15
CATGGGA43200.021.9974884
GTGTACG1954.1858766E-721.943091
AGTACTT74500.021.77241112-13
CAGAGTA122350.020.94474410-11
Produced by FastQC (version 0.11.5)