##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937817_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1407450 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.845514227858892 33.0 31.0 34.0 30.0 34.0 2 31.97177803829621 33.0 31.0 34.0 30.0 34.0 3 31.981251198976874 34.0 31.0 34.0 30.0 34.0 4 35.56925432519805 37.0 35.0 37.0 33.0 37.0 5 35.56897864933035 37.0 35.0 37.0 33.0 37.0 6 35.621689580446905 37.0 35.0 37.0 33.0 37.0 7 35.566629009911544 37.0 35.0 37.0 33.0 37.0 8 35.5527443248428 37.0 35.0 37.0 33.0 37.0 9 37.19407652136843 39.0 37.0 39.0 34.0 39.0 10-11 37.084025365021844 39.0 37.0 39.0 33.5 39.0 12-13 36.96852037372553 39.0 37.0 39.0 33.0 39.0 14-15 38.144461259725034 40.0 38.0 41.0 33.0 41.0 16-17 38.08391523677573 40.0 38.0 41.0 33.0 41.0 18-19 38.04870190770542 40.0 38.0 41.0 33.0 41.0 20-21 37.98169100145654 40.0 38.0 41.0 33.0 41.0 22-23 37.793325873032785 40.0 38.0 41.0 33.0 41.0 24-25 37.63323137589258 40.0 37.0 41.0 32.0 41.0 26-27 37.57625990266084 40.0 37.0 41.0 32.5 41.0 28-29 37.611699527514304 40.0 37.0 41.0 33.0 41.0 30-31 37.41490070695229 40.0 37.0 41.0 32.0 41.0 32-33 37.1568034388433 39.5 36.0 41.0 31.5 41.0 34-35 37.16311662936516 40.0 36.0 41.0 31.5 41.0 36-37 37.080254005470884 40.0 36.0 41.0 31.0 41.0 38-39 36.94538385022558 40.0 36.0 41.0 31.0 41.0 40-41 36.70865181711606 39.5 36.0 41.0 30.5 41.0 42-43 36.43459057160112 39.0 35.5 41.0 30.0 41.0 44-45 36.15622402216775 39.0 35.0 41.0 29.5 41.0 46-47 35.741185832534015 39.0 35.0 41.0 27.5 41.0 48-49 35.33838075952964 39.0 35.0 41.0 26.0 41.0 50-51 34.387344488258904 38.0 34.0 39.5 24.5 40.5 52-53 34.480892394045966 38.0 34.5 39.5 24.0 40.5 54-55 34.83674695371061 39.0 35.0 40.0 24.0 41.0 56-57 34.64539557355501 38.5 34.5 40.0 23.5 41.0 58-59 34.414986678034744 38.0 34.0 40.0 23.0 41.0 60-61 34.11160716188852 38.0 34.0 40.0 22.0 41.0 62-63 33.76309069593947 38.0 34.0 40.0 21.5 41.0 64-65 33.399558066005895 37.0 33.0 40.0 20.0 41.0 66-67 33.000292017478415 36.5 33.0 39.5 18.5 41.0 68-69 32.522487832605066 36.0 32.0 39.0 15.0 40.5 70-71 32.09578528544531 35.5 32.0 39.0 12.0 40.0 72-73 31.63727095101069 35.0 31.0 38.0 8.0 40.0 74-75 31.187798856087248 35.0 31.0 37.0 7.0 39.5 76-77 30.70540090234111 35.0 30.5 37.0 2.0 39.0 78-79 30.1771569149881 34.5 30.0 36.0 2.0 39.0 80-81 29.6546985683328 34.0 29.5 36.0 2.0 37.5 82-83 29.14678674198018 34.0 29.0 35.0 2.0 37.0 84-85 28.693939038686985 34.0 29.0 35.0 2.0 36.5 86-87 28.23279974421827 34.0 27.5 35.0 2.0 36.0 88-89 27.76722512345021 34.0 27.0 35.0 2.0 36.0 90-91 27.294313829976197 33.0 26.0 35.0 2.0 35.0 92-93 26.758269565526305 33.0 25.0 35.0 2.0 35.0 94-95 26.14247610927564 32.5 24.5 35.0 2.0 35.0 96-97 25.44057586415148 32.0 21.5 34.5 2.0 35.0 98-99 24.535874453799423 32.0 10.0 34.0 2.0 35.0 100-101 22.177869196063803 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23571572737379753 1101 2 0.3120827560459638 1101 3 0.28710624342620505 1101 4 0.2417291585762129 1101 5 0.20410741015835043 1101 6 0.29290955362544224 1101 7 0.27806722195869327 1101 8 0.3001729264470896 1101 9 0.38963869156140873 1101 10-11 0.3694125958526442 1101 12-13 0.49855967820355573 1101 14-15 0.5682165256268021 1101 16-17 0.6920762174878163 1101 18-19 0.5553831439046348 1101 20-21 0.5809404483236946 1101 22-23 0.5654744937642491 1101 24-25 0.6081513629563986 1101 26-27 0.5669004636884694 1101 28-29 0.35405814574798455 1101 30-31 0.43803769938224946 1101 32-33 0.4617786071505421 1101 34-35 0.6029110479857636 1101 36-37 0.6988563087765769 1101 38-39 0.7543138722947091 1101 40-41 0.8165512304412275 1101 42-43 0.9037181864720978 1101 44-45 0.8032814291484627 1101 46-47 1.0348987350387944 1101 48-49 1.160215365189984 1101 50-51 1.149005147081688 1101 52-53 1.1216975248033236 1101 54-55 1.3923950694116343 1101 56-57 1.4179994111036862 1101 58-59 1.3841397451261628 1101 60-61 1.4614700507045342 1101 62-63 1.5759590431488206 1101 64-65 1.3306891900205997 1101 66-67 1.487266792536225 1101 68-69 1.3537237999303002 1101 70-71 1.3089571723850675 1101 72-73 1.3062994893747621 1101 74-75 1.396701604371799 1101 76-77 1.5735837196663454 1101 78-79 1.5976944852893986 1101 80-81 1.5542291666247188 1101 82-83 1.6582339606064096 1101 84-85 1.7689739654564534 1101 86-87 1.8423054634691418 1101 88-89 1.7749901854528467 1101 90-91 1.8287780565679022 1101 92-93 1.9993638970030112 1101 94-95 1.9430229033535475 1101 96-97 2.044135157796269 1101 98-99 2.284127578519403 1101 100-101 2.606791550519201 1102 1 0.28204951556900326 1102 2 0.27342442264980704 1102 3 0.36063644901874525 1102 4 0.2709759223973478 1102 5 0.25966036889659705 1102 6 0.21915226892463124 1102 7 0.1522602693573134 1102 8 0.2514401816698282 1102 9 0.3199061051062415 1102 10-11 0.3202596190204474 1102 12-13 0.47970170819427693 1102 14-15 0.5178838230687717 1102 16-17 0.5993513435800537 1102 18-19 0.48123322042697936 1102 20-21 0.477970476746286 1102 22-23 0.5450381279346814 1102 24-25 0.6138910663663566 1102 26-27 0.5383757993689855 1102 28-29 0.5002845764896051 1102 30-31 0.5980716255438381 1102 32-33 0.6259721822894448 1102 34-35 0.7386535408801151 1102 36-37 0.8017753157201355 1102 38-39 0.7641984479538166 1102 40-41 0.8159637801053634 1102 42-43 0.932477619829811 1102 44-45 0.9218587921056169 1102 46-47 1.2171950084384235 1102 48-49 1.3035576198472896 1102 50-51 1.2927166691600789 1102 52-53 1.1959273632394058 1102 54-55 1.2236127170469828 1102 56-57 1.2325377514358635 1102 58-59 1.1704488049191326 1102 60-61 1.3164982848101232 1102 62-63 1.4539073396785582 1102 64-65 1.361727707522725 1102 66-67 1.4095402977118496 1102 68-69 1.382876776762501 1102 70-71 1.2919669506587574 1102 72-73 1.414723129124166 1102 74-75 1.476267349503388 1102 76-77 1.6005898489928754 1102 78-79 1.694326899098101 1102 80-81 1.621706040291233 1102 82-83 1.6031630549286753 1102 84-85 1.6780244098891686 1102 86-87 1.7856516384829 1102 88-89 1.906072430007697 1102 90-91 1.9731821246916361 1102 92-93 2.1765042985379814 1102 94-95 2.1639545911423888 1102 96-97 2.2606339779197597 1102 98-99 2.5492199707661456 1102 100-101 3.049330886018783 1103 1 0.13116420727271816 1103 2 0.1255400807296958 1103 3 0.16529845570104484 1103 4 0.0707958334610268 1103 5 -0.04960693719206688 1103 6 0.08763689079048476 1103 7 0.09733128655336287 1103 8 0.17919938375636235 1103 9 0.15523956679447082 1103 10-11 0.2188139549293382 1103 12-13 0.22212887246522683 1103 14-15 0.3112661273033055 1103 16-17 0.36919858061708766 1103 18-19 0.3163976746225501 1103 20-21 0.18832707636505575 1103 22-23 0.279426602490382 1103 24-25 0.3055054779386026 1103 26-27 0.19481962790252538 1103 28-29 0.03586993982166575 1103 30-31 0.18685921655561089 1103 32-33 0.23764806182487064 1103 34-35 0.22255201752209786 1103 36-37 0.28681892646306295 1103 38-39 0.31292135800283205 1103 40-41 0.538558565140832 1103 42-43 0.42417845428109047 1103 44-45 0.28105759753837134 1103 46-47 0.590222093591585 1103 48-49 0.5547446962838691 1103 50-51 0.7001736140370198 1103 52-53 0.5443039626972208 1103 54-55 0.4877027107509875 1103 56-57 0.5298005729447581 1103 58-59 0.6022575317726933 1103 60-61 0.6246840667607501 1103 62-63 0.8011001128438764 1103 64-65 0.7229247324955921 1103 66-67 0.6781578969016095 1103 68-69 0.8583381888851065 1103 70-71 0.7645991897356375 1103 72-73 0.8517698501002222 1103 74-75 0.8852305040127675 1103 76-77 0.9531743016655128 1103 78-79 0.9222031682793705 1103 80-81 0.907537485961516 1103 82-83 1.1799226365082234 1103 84-85 1.0396431797498735 1103 86-87 1.0622379719055886 1103 88-89 1.0355563133169987 1103 90-91 1.3034309681046459 1103 92-93 1.3692748327935682 1103 94-95 1.5103981287106514 1103 96-97 1.8025914808928718 1103 98-99 1.7360134900407367 1103 100-101 2.259204142780785 1104 1 0.16538733988922516 1104 2 0.19801492726302783 1104 3 0.1720086446410498 1104 4 0.1576606970620844 1104 5 0.10887029414708138 1104 6 0.16714600526896106 1104 7 0.11322999205582107 1104 8 0.18874769945477254 1104 9 0.3012667088982397 1104 10-11 0.16102495309267084 1104 12-13 0.27460336829181387 1104 14-15 0.31974868418924274 1104 16-17 0.37057348092545084 1104 18-19 0.4168923659616439 1104 20-21 0.3519444345301608 1104 22-23 0.23638546848663822 1104 24-25 0.2600639511433087 1104 26-27 0.25182928143025407 1104 28-29 0.28542278872941296 1104 30-31 0.3081439982211833 1104 32-33 0.25649530176283974 1104 34-35 0.3216179036281446 1104 36-37 0.45963551722987006 1104 38-39 0.5316526049341448 1104 40-41 0.4970313152272112 1104 42-43 0.5723669890283318 1104 44-45 0.5767962508764555 1104 46-47 0.6822433204416853 1104 48-49 0.5237847599826111 1104 50-51 0.5887797365973384 1104 52-53 0.5321646386000367 1104 54-55 0.5300602913421031 1104 56-57 0.5180285167381555 1104 58-59 0.5190120916097527 1104 60-61 0.5703417328203813 1104 62-63 0.5725006926790215 1104 64-65 0.4362333388389672 1104 66-67 0.6212664445340792 1104 68-69 0.5126657152075964 1104 70-71 0.4080460394798813 1104 72-73 0.44602488404083473 1104 74-75 0.4751585311964348 1104 76-77 0.4525186749006167 1104 78-79 0.359786136401425 1104 80-81 0.49557656768137903 1104 82-83 0.6859882394510421 1104 84-85 0.8909610577402951 1104 86-87 0.9287719761712196 1104 88-89 0.9348060606782553 1104 90-91 1.1079382641037299 1104 92-93 1.1514541244698684 1104 94-95 1.152770127245475 1104 96-97 1.5140173116132232 1104 98-99 1.6356415460602953 1104 100-101 2.1725565225208463 1105 1 0.2889270178257597 1105 2 0.16132224589008715 1105 3 0.31947957343217936 1105 4 0.34458492635011595 1105 5 0.2723117933634853 1105 6 0.22940174175210615 1105 7 0.2915451767006303 1105 8 0.3029664844367659 1105 9 0.2540837499833444 1105 10-11 0.3757922181376543 1105 12-13 0.33002517316349156 1105 14-15 0.5426064151999768 1105 16-17 0.4173075434118303 1105 18-19 0.4735387753161291 1105 20-21 0.5822600444057286 1105 22-23 0.5269035362861487 1105 24-25 0.45196754997790123 1105 26-27 0.29113251153118114 1105 28-29 0.42430101345861715 1105 30-31 0.4898523890908919 1105 32-33 0.48014564487748146 1105 34-35 0.6163709993230881 1105 36-37 0.6231491403912699 1105 38-39 0.5321732496621152 1105 40-41 0.6734632009227752 1105 42-43 0.6549947348094634 1105 44-45 0.6060676788253829 1105 46-47 0.9489285565198884 1105 48-49 0.8803217813538353 1105 50-51 0.8308256299650907 1105 52-53 0.8042103366320958 1105 54-55 0.7447755294788365 1105 56-57 0.9294385248370745 1105 58-59 0.9654983782934181 1105 60-61 1.007733452991502 1105 62-63 1.0511405477699967 1105 64-65 1.0826680493890493 1105 66-67 1.1211344051243586 1105 68-69 1.1032062466108172 1105 70-71 1.315226278721017 1105 72-73 1.2098174615853416 1105 74-75 1.2347331727178954 1105 76-77 1.47340475512949 1105 78-79 1.4252282862359174 1105 80-81 1.3761532136474735 1105 82-83 1.3564785395317323 1105 84-85 1.2782827951460263 1105 86-87 1.4344720216994595 1105 88-89 1.5637681820838907 1105 90-91 1.5413733097296394 1105 92-93 1.8035980838640917 1105 94-95 1.9219321822369793 1105 96-97 2.064382927423992 1105 98-99 2.3226594989122695 1105 100-101 2.7609119237779076 1106 1 0.15373748092481065 1106 2 0.11772183241503953 1106 3 0.1349825872291781 1106 4 0.1981163423021144 1106 5 0.20107286349771414 1106 6 0.07030186512449887 1106 7 0.03730953293128891 1106 8 -0.0828820470716991 1106 9 0.14704132178263052 1106 10-11 0.013057631810234227 1106 12-13 0.10762730795095621 1106 14-15 0.07972990311303363 1106 16-17 0.019507592720778177 1106 18-19 0.20462071668848125 1106 20-21 0.2244325987243414 1106 22-23 0.04590998595676865 1106 24-25 -0.020517206005301603 1106 26-27 -0.03807273922183185 1106 28-29 0.1481928998359905 1106 30-31 0.21278610155970057 1106 32-33 0.3762690412739218 1106 34-35 0.29963601192287825 1106 36-37 0.3385574870061774 1106 38-39 0.3393622544664865 1106 40-41 0.4818744275604061 1106 42-43 0.4926965276848705 1106 44-45 0.45128833637473065 1106 46-47 0.4658883847287143 1106 48-49 0.5648375209605732 1106 50-51 0.4350245420676302 1106 52-53 0.5220456237367088 1106 54-55 0.4633954589960467 1106 56-57 0.3725026909832323 1106 58-59 0.47802390167591113 1106 60-61 0.5048234248213888 1106 62-63 0.5555513483302903 1106 64-65 0.5238118669919416 1106 66-67 0.5748900117669606 1106 68-69 0.40030166943005696 1106 70-71 0.4122886455405954 1106 72-73 0.34098576863307883 1106 74-75 0.699851341665557 1106 76-77 0.777729740114264 1106 78-79 0.6967043091340699 1106 80-81 0.8767955802370082 1106 82-83 0.5318456186565932 1106 84-85 0.49285949081629354 1106 86-87 0.7110064627004995 1106 88-89 0.8880237844757239 1106 90-91 1.1122396719743826 1106 92-93 1.1812233728540384 1106 94-95 1.3656619691933116 1106 96-97 1.5349363328670833 1106 98-99 1.5266897804083612 1106 100-101 2.0744358283665925 1107 1 0.16374138512548697 1107 2 0.26775355404553025 1107 3 0.2714789331413634 1107 4 0.2835817352233079 1107 5 0.17354641863845188 1107 6 0.2287630000268308 1107 7 0.15474910932551467 1107 8 0.2523277427114934 1107 9 0.33043016796931823 1107 10-11 0.30729039537428093 1107 12-13 0.292191989575592 1107 14-15 0.592690324278685 1107 16-17 0.5728481244241053 1107 18-19 0.3550178471009815 1107 20-21 0.4842650841140923 1107 22-23 0.4164094796307367 1107 24-25 0.49896567041454176 1107 26-27 0.5223505615358235 1107 28-29 0.3473403810165223 1107 30-31 0.47822904532701216 1107 32-33 0.5030430124560468 1107 34-35 0.5452148096730767 1107 36-37 0.4420276503725802 1107 38-39 0.5630494669892059 1107 40-41 0.4515631895627763 1107 42-43 0.3709382343085821 1107 44-45 0.4708026294093912 1107 46-47 0.5276830359737872 1107 48-49 0.6089629190060322 1107 50-51 0.42677966328086114 1107 52-53 0.5074835652469005 1107 54-55 0.6483170089637156 1107 56-57 0.6173677520473646 1107 58-59 0.595480253932422 1107 60-61 0.6604046016938838 1107 62-63 0.6439760033575652 1107 64-65 0.6832484420319034 1107 66-67 0.576214389839997 1107 68-69 0.7057666353293044 1107 70-71 0.927781916894741 1107 72-73 0.8910961843084415 1107 74-75 0.846760312786671 1107 76-77 1.0894197709818485 1107 78-79 0.9526368640673937 1107 80-81 0.8598934414670758 1107 82-83 0.8806975396453289 1107 84-85 1.1540663768502348 1107 86-87 1.1532578444215211 1107 88-89 1.2938084068053115 1107 90-91 1.5280278934889715 1107 92-93 1.5969057539284286 1107 94-95 1.6587545841537192 1107 96-97 1.9391025420102324 1107 98-99 2.1599386243989436 1107 100-101 2.705560848192608 1108 1 0.010740655976345437 1108 2 -0.09403903106860767 1108 3 -0.2164452353481785 1108 4 -0.07541203896481363 1108 5 -0.02952442100482955 1108 6 -0.16565620463972408 1108 7 -0.04423008187982447 1108 8 -0.1362610732624816 1108 9 -0.22950308684026055 1108 10-11 -0.02215040332446705 1108 12-13 0.05574855472226403 1108 14-15 -0.0023977345019545737 1108 16-17 -0.02252177854296633 1108 18-19 0.1369671992800221 1108 20-21 0.2643733347170425 1108 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-1.5309104359411627 2316 92-93 -1.6557809491681112 2316 94-95 -1.3007822360388381 2316 96-97 -0.9711043276228679 2316 98-99 -0.9709421978753205 2316 100-101 -0.9223730576101481 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5918.0 3 1104.0 4 945.0 5 1108.0 6 1322.0 7 1433.0 8 1766.0 9 2231.0 10 2802.0 11 3611.0 12 5091.0 13 6928.0 14 7731.0 15 10193.0 16 13377.0 17 18361.0 18 15496.0 19 9524.0 20 9107.0 21 9544.0 22 10121.0 23 11132.0 24 12220.0 25 14088.0 26 16075.0 27 18757.0 28 22433.0 29 26543.0 30 32470.0 31 40665.0 32 52011.0 33 68046.0 34 93418.0 35 133494.0 36 197396.0 37 277599.0 38 225062.0 39 28322.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.70548620892186 18.930870688327907 15.221293059697663 22.14235004305257 2 14.890899839367847 19.91938460924483 41.78752086293816 23.402194688449164 3 18.785921229455514 24.974241459066366 30.61611009692052 25.6237272145576 4 12.188055024995494 16.680333387240292 38.246275967065934 32.88533562069828 5 13.16279344788306 37.204414906614595 34.690200676925755 14.942590968576596 6 30.638764198557645 36.51920294395771 18.0096669818737 14.83236587561095 7 27.60353550816166 33.478961106277424 21.0554121270721 17.86209125848882 8 25.89144438415318 34.6829794900498 20.280083800115932 19.14549232568109 9 26.28463311049269 16.834176937559995 21.121675548959903 35.75951440298741 10-11 24.471899878618956 26.886987446372828 27.540038119843572 21.10107455516464 12-13 25.50786362594121 24.423063502891804 28.520758178014315 21.54831469315267 14-15 22.456654663580093 25.459337022318334 27.440487168185392 24.643521145916186 16-17 21.00582857350372 28.974429541407936 28.07808743323095 21.941654451857392 18-19 20.934977730148503 26.80400038451542 31.50206677039089 20.758955114945188 20-21 21.736671045277685 25.81009643623781 31.681991805233682 20.771240713250815 22-23 21.38369279716145 25.82319654948897 31.64253451810276 21.15057613524682 24-25 21.24026013319902 25.977190290923456 31.74543396468197 21.037115611195553 26-27 21.02778269257264 26.214863122864212 31.658656045488414 21.09869813907473 28-29 20.73211027728839 26.201680337149863 31.959484191471272 21.10672519409048 30-31 20.834070630256242 26.0978900075231 31.883697804273393 21.184341557947263 32-33 20.806468152743772 26.147992428612326 31.68328782809703 21.362251590546872 34-35 21.2647013377443 26.116774745278875 31.38835741633847 21.23016650063836 36-37 21.229955528121796 26.019199121740034 31.377716929891914 21.373128420246257 38-39 21.009701023925775 26.22394798781712 31.49127078714561 21.275080201111496 40-41 21.041567074101852 26.135746786727097 31.541064647039203 21.281621492131848 42-43 21.07442444471896 26.128460015599952 31.190363202625292 21.606752337055795 44-45 21.165974388804322 26.48635640657239 30.805095184052877 21.542574020570406 46-47 21.31089999512298 26.654368509693345 30.305369028366098 21.72936246681758 48-49 21.388493106986434 26.784418606472688 30.04575642288163 21.781331863659243 50-51 21.39834393521016 27.02565280322169 29.82109444126952 21.75490882029863 52-53 21.543054520305287 27.185757077283423 29.560396629157864 21.710791773253423 54-55 21.2755254255632 27.090612368122933 29.60906643463946 22.024795771674405 56-57 21.426011467911948 27.433941702348925 29.557366786333176 21.58268004340595 58-59 21.443933876849957 26.857982999771863 29.855711960628202 21.842371162749977 60-61 21.486024825928766 26.845446954278408 30.146731675091708 21.52179654470112 62-63 21.399834402314095 26.897663335490517 30.11556952860191 21.58693273359348 64-65 21.30154769891677 26.860308472036603 29.98343212956751 21.854711699479118 66-67 21.186237669682573 27.159712070425496 29.721114942246434 21.932935317645494 68-69 21.177195481640158 26.98909849936177 29.92067882184344 21.913027197154634 70-71 21.237576698525988 27.09796874149104 29.72295454877117 21.941500011211804 72-73 21.347602726926816 26.999003012319434 29.700907255229914 21.95248700552383 74-75 21.149589841539232 27.260534448256244 29.47125402487789 22.118621685326627 76-77 21.30858708878587 27.1175440682564 29.52916725111056 22.04470159184717 78-79 21.34121289411155 27.06275949823162 29.43912423374369 22.156903373913142 80-81 21.15755459889494 27.432996674708455 29.330203479648297 22.079245246748307 82-83 21.3239005823263 27.254494179228917 29.367821043061106 22.05378419538368 84-85 21.191054190278212 26.94243528775237 29.37391434348037 22.492596178489052 86-87 21.201406635277582 26.98478636878007 29.53357417993441 22.280232816007935 88-89 21.27530738372389 26.789827813852853 29.448035339294176 22.486829463129084 90-91 21.0759769675071 26.969767628133816 29.675640557834875 22.27861484652421 92-93 21.197888633535243 26.723281132972797 29.712663072730223 22.366167160761734 94-95 20.915771843735705 26.784493176919533 29.690785504135498 22.60894947520927 96-97 21.036974393307943 26.775068792424666 29.57246722682974 22.615489587437654 98-99 20.994515846452245 26.924036624620424 29.31464632424315 22.766801204684185 100-101 21.03536925533895 26.489800814141013 29.925252359154562 22.54957757136547 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2132.0 1 1263.5 2 404.0 3 431.5 4 375.5 5 330.0 6 626.5 7 1418.5 8 2469.0 9 3052.5 10 2751.5 11 2379.5 12 2428.0 13 2563.5 14 2726.0 15 2871.0 16 3005.0 17 3153.5 18 3254.5 19 3396.0 20 3597.5 21 3988.5 22 4668.5 23 5470.5 24 6516.0 25 8039.0 26 9957.0 27 12111.5 28 13720.5 29 15568.5 30 18153.0 31 20823.5 32 23851.5 33 27279.5 34 31490.0 35 34514.0 36 37834.5 37 41613.5 38 43253.5 39 43703.0 40 44175.0 41 45913.5 42 47769.5 43 49788.0 44 51365.0 45 52146.5 46 51724.0 47 51949.0 48 55896.0 49 55939.5 50 51631.5 51 48545.0 52 45803.5 53 43559.5 54 41172.0 55 37804.0 56 33567.0 57 28994.5 58 25132.0 59 21839.5 60 18517.5 61 14850.0 62 11398.5 63 8571.0 64 6195.5 65 4560.0 66 3310.5 67 2541.0 68 1871.5 69 1203.0 70 891.0 71 684.0 72 539.0 73 420.0 74 308.0 75 239.0 76 171.5 77 101.5 78 64.0 79 48.0 80 31.5 81 22.0 82 11.0 83 11.5 84 13.0 85 10.5 86 7.0 87 4.0 88 3.5 89 2.5 90 3.0 91 2.5 92 2.5 93 2.5 94 1.0 95 0.5 96 1.0 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3207218728906888 2 0.3015382429215958 3 0.28647554087178945 4 0.26977867775054176 5 0.26651035560765923 6 0.21897758357312871 7 0.21002522292088527 8 0.22423531919428755 9 0.22437742015702158 10-11 0.22697076272691746 12-13 0.22281430956694734 14-15 0.2194749369426978 16-17 0.21851575544424312 18-19 0.21919073501722974 20-21 0.2056911435574976 22-23 0.1981242672919109 24-25 0.19787559060712637 26-27 0.21034495008703685 28-29 0.22792994422537213 30-31 0.2208604213293545 32-33 0.2284983480763082 34-35 0.2181960282780916 36-37 0.20281359906213367 38-39 0.20292017478418414 40-41 0.1967032576645707 42-43 0.21020284912430282 44-45 0.20661479981526876 46-47 0.2062950726491172 48-49 0.21720132153895344 50-51 0.22860492379835873 52-53 0.24643859462147857 54-55 0.214359302284273 56-57 0.18284841379800348 58-59 0.18377207005577462 60-61 0.1924757540232335 62-63 0.201854417563679 64-65 0.18632988738498701 66-67 0.19407438985399125 68-69 0.1975913886816583 70-71 0.19045081530427369 72-73 0.1931507335962201 74-75 0.19531777327791394 76-77 0.21180148495506057 78-79 0.21720132153895344 80-81 0.20686347650005327 82-83 0.20682795125936976 84-85 0.19680983338662117 86-87 0.2004689331770223 88-89 0.2056556183168141 90-91 0.20693452698142029 92-93 0.20334647767238623 94-95 0.20196099328572953 96-97 0.19045081530427369 98-99 0.19656115670183666 100-101 0.18874560375146543 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1407450.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.15564584484457 #Duplication Level Percentage of deduplicated Percentage of total 1 75.80074521375988 41.80839057785437 2 13.512720848660026 14.9060569105788 3 4.607342101660691 7.623627877357167 4 2.075612442424712 4.57926979142121 5 1.115347726204862 3.0758862090204016 6 0.6499744935623204 2.150985778506333 7 0.4223901965311529 1.6308042861744594 8 0.28663421468242944 1.264759618563138 9 0.23068824605369626 1.1451383279915428 >10 1.161185935938486 11.673882613701059 >50 0.09226729876891766 3.5348963696787536 >100 0.042643503921239875 4.129459219195826 >500 0.001674795358497091 0.6103817421875345 >1k 5.153216487683357E-4 0.6888999988950113 >5k 2.5766082438416786E-4 1.1775606788744843 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9334 0.6631851930796832 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7238 0.5142633841344275 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4676 0.33223205087214464 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2031 0.14430352765639987 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1774 0.12604355394507796 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 7.105048136701127E-5 0.0 6 0.0 0.0 0.0 7.105048136701127E-5 0.0 7 0.0 0.0 0.0 7.105048136701127E-5 0.0 8 0.0 0.0 0.0 7.105048136701127E-5 0.0 9 0.0 0.0 0.0 7.105048136701127E-5 0.0 10-11 0.0 0.0 0.0 7.105048136701127E-5 0.0 12-13 0.0 0.0 0.0 1.065757220505169E-4 0.0 14-15 0.0 0.0 0.0 1.4210096273402253E-4 0.0 16-17 0.0 0.0 0.0 2.1315144410103377E-4 0.0 18-19 0.0 0.0 0.0 2.1315144410103377E-4 0.0 20-21 0.0 0.0 0.0 2.4867668478453943E-4 0.0 22-23 0.0 0.0 0.0 3.907776475185619E-4 0.0 24-25 0.0 0.0 0.0 7.105048136701127E-4 0.0 26-27 0.0 0.0 0.0 0.0011012824611886746 0.0 28-29 0.0 0.0 0.0 0.002060463959643327 0.0 30-31 0.0 0.0 0.0 0.0042275036413371705 0.0 32-33 0.0 0.0 0.0 0.007033997655334115 0.0 34-35 0.0 0.0 0.0 0.009947067391381577 0.0 36-37 0.0 0.0 0.0 0.014032470069984725 0.0 38-39 0.0 0.0 0.0 0.0208177910405343 0.0 40-41 0.0 0.0 0.0 0.028846495435006572 0.0 42-43 0.0 0.0 0.0 0.04017904721304487 0.0 44-45 0.0 0.0 0.0 0.050445841770577995 0.0 46-47 0.0 0.0 0.0 0.0694518455362535 0.0 48-49 0.0 0.0 0.0 0.0940353120892394 0.0 50-51 0.0 0.0 0.0 0.10568759103342926 0.0 52-53 0.0 0.0 0.0 0.12636328111122952 0.0 54-55 0.0 0.0 0.0 0.15862019965185264 0.0 56-57 0.0 0.0 0.0 0.2119080606771111 0.0 58-59 0.0 0.0 0.0 0.2794770684571388 0.0 60-61 0.0 0.0 0.0 0.35411559913318413 0.0 62-63 0.0 0.0 0.0 0.43710256136985326 0.0 64-65 0.0 0.0 0.0 0.5244591282105937 0.0 66-67 0.0 0.0 0.0 0.6360439091974848 0.0 68-69 0.0 0.0 0.0 0.7677004511705567 0.0 70-71 0.0 0.0 0.0 0.8585029663575972 0.0 72-73 0.0 0.0 0.0 0.9736757966535223 0.0 74-75 0.0 0.0 0.0 1.1064691463284664 0.0 76-77 0.0 0.0 0.0 1.2554975309957723 0.0 78-79 0.0 0.0 0.0 1.4244911009272088 0.0 80-81 0.0 0.0 0.0 1.6669508685921346 0.0 82-83 0.0 0.0 0.0 1.9131763117695124 0.0 84-85 0.0 0.0 0.0 2.238907243596575 0.0 86-87 0.0 0.0 0.0 2.630501971650858 0.0 88-89 0.0 0.0 0.0 3.1149952040925077 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4845 0.0 45.531727 1 GTATCAA 8340 0.0 38.93539 1 ATCAACG 10275 0.0 31.257965 3 TATCAAC 10270 0.0 31.23471 2 TCAACGC 10310 0.0 31.061903 4 CAACGCA 10480 0.0 30.512695 5 AACGCAG 10730 0.0 29.795406 6 ACGCAGA 11890 0.0 26.768679 7 CGCAGAG 11840 0.0 26.761354 8 GTACATG 6605 0.0 25.625189 1 TACATGG 6730 0.0 24.785416 2 GCAGAGT 12365 0.0 24.779018 9 GAGTACT 7225 0.0 23.962482 12-13 ACATGGG 6800 0.0 23.825462 3 GTAGCGC 100 0.001651563 23.755592 3 GTACTTT 7665 0.0 22.308094 14-15 CATGGGA 4320 0.0 21.997488 4 GTGTACG 195 4.1858766E-7 21.94309 1 AGTACTT 7450 0.0 21.772411 12-13 CAGAGTA 12235 0.0 20.944744 10-11 >>END_MODULE