FastQCFastQC Report
Thu 26 May 2016
SRR937817_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937817_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407450
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80680.5732352836690469No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66040.46921737894774235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42850.30445131265764325No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17180.12206472698852536No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14200.10089168354115599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61750.032.86471
GTATCAA91800.031.5810031
TATCAAC113500.025.238272
ATCAACG114000.025.0859033
TCAACGC114000.025.0859034
CAACGCA116800.024.484535
AACGCAG119950.023.8769056
GTACATG71600.022.9004941
AGTACTT70250.022.414512-13
GAGTACT69100.022.30634712-13
TACATGG74150.021.7824082
GTACTTT71700.021.72779514-15
ACGCAGA135300.021.0291067
CGCAGAG135550.020.9202388
ACATGGG73850.020.7130243
GCAGAGT141900.019.6493199
AGAGTAC118550.019.57288610-11
TACTTTT81450.019.27264614-15
TACACCG1759.930974E-519.0019475
CATTCCG2001.2851146E-518.9999229
Produced by FastQC (version 0.11.5)