FastQCFastQC Report
Thu 26 May 2016
SRR937816_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937816_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073420
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78590.7321458515772018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59350.5529056660021241No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40170.3742244415047232No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16690.15548433977380707No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14810.13797022600659575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36150.047.512831
GTATCAA63150.040.458791
ATCAACG79100.032.16073
TATCAAC79000.032.142722
TCAACGC79650.031.8774364
AATACCG600.003933338431.6953875
CAACGCA80700.031.400825
AACGCAG82250.030.7860726
GTATTAC1554.5138222E-827.6263031
CGCAGAG92350.027.2647768
ACGCAGA92400.027.2500217
GCAGAGT95550.025.654399
GAGTACT60200.024.10328912-13
GTACATG46200.023.5833471
TACATGG47400.022.6724552
GTACTTT64350.022.36327714-15
AGTACTT61900.021.90670612-13
CAGAGTA94800.021.26821110-11
ACATGGG47250.021.1332193
AGAGTAC87300.020.91913410-11
Produced by FastQC (version 0.11.5)