FastQCFastQC Report
Thu 26 May 2016
SRR937816_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937816_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073420
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59050.5501108606137393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49470.46086340854465163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34080.31748989212051204No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12320.11477334128300198No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11550.10760000745281438No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG11090.10331463919062435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45250.032.77061
GTATCAA68150.032.5686231
ATCAACG85050.026.026583
TCAACGC85050.026.026584
TATCAAC85200.025.980762
CAACGCA86550.025.5206325
AACGCAG88550.024.9955356
GTACATG56400.024.0167431
GCACCGT1801.9520121E-723.7484386
TACATGG58400.022.9372732
GAGTACT52150.022.68039312-13
ACATGGG57150.022.0259763
ACGCAGA100350.021.9637327
TGCGCCG1302.940787E-421.923685
CGCAGAG100250.021.843498
AGTACTT53900.021.67963212-13
GCGAACT550.00287926821.5894966-67
GTACTTT55150.021.48770914-15
GCAGAGT104450.020.6013329
TGCACCG1656.3208834E-520.152075
Produced by FastQC (version 0.11.5)