##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937816_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1073420 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.32364964319651 33.0 31.0 34.0 31.0 34.0 2 32.5510107879488 34.0 31.0 34.0 31.0 34.0 3 32.64660431145311 34.0 31.0 34.0 31.0 34.0 4 36.230020867880235 37.0 35.0 37.0 35.0 37.0 5 36.092765180451266 37.0 35.0 37.0 35.0 37.0 6 36.18798513163534 37.0 36.0 37.0 35.0 37.0 7 36.148433045778916 37.0 36.0 37.0 35.0 37.0 8 36.14430418661847 37.0 36.0 37.0 35.0 37.0 9 37.832118835125115 39.0 38.0 39.0 35.0 39.0 10-11 37.77996962978145 39.0 37.5 39.0 35.0 39.0 12-13 37.70829218758733 39.0 37.0 39.0 35.0 39.0 14-15 38.96551769111811 40.0 38.0 41.0 36.0 41.0 16-17 38.94293845838535 40.0 38.0 41.0 36.0 41.0 18-19 38.93573624489948 40.0 38.0 41.0 35.5 41.0 20-21 38.866977511132646 40.0 38.0 41.0 35.0 41.0 22-23 38.717631961394424 40.0 38.0 41.0 34.5 41.0 24-25 38.66112472284846 40.0 38.0 41.0 34.5 41.0 26-27 38.59161325483035 40.0 38.0 41.0 34.0 41.0 28-29 38.48000363324701 40.0 38.0 41.0 34.0 41.0 30-31 38.55112118276164 40.0 38.0 41.0 35.0 41.0 32-33 38.466515902442666 40.0 38.0 41.0 34.0 41.0 34-35 38.34269204971027 40.0 38.0 41.0 34.0 41.0 36-37 38.186306385198705 40.0 38.0 41.0 34.0 41.0 38-39 38.059193512325095 40.0 38.0 41.0 34.0 41.0 40-41 37.87340276872054 40.0 37.0 41.0 33.0 41.0 42-43 37.66435132566936 40.0 37.0 41.0 33.0 41.0 44-45 37.346012744312574 40.0 37.0 41.0 33.0 41.0 46-47 36.95730748448884 40.0 37.0 41.0 31.5 41.0 48-49 36.603259208883756 40.0 36.0 41.0 31.0 41.0 50-51 36.33701999217455 39.5 36.0 41.0 30.0 41.0 52-53 36.126640550762986 39.0 36.0 41.0 30.0 41.0 54-55 35.913341469322354 39.0 35.0 40.5 29.0 41.0 56-57 35.64969117400459 39.0 35.0 40.0 28.5 41.0 58-59 35.401871122207524 38.5 35.0 40.0 28.0 41.0 60-61 35.06625179333346 38.0 34.5 40.0 27.5 41.0 62-63 34.71727562370741 38.0 34.0 40.0 26.5 41.0 64-65 34.315784595032696 37.0 34.0 40.0 26.0 41.0 66-67 33.895733263773735 36.5 33.5 39.0 26.0 41.0 68-69 33.45292709284344 36.0 33.0 39.0 25.0 40.0 70-71 33.049869575748545 35.5 33.0 39.0 24.0 40.0 72-73 32.60220137504425 35.0 32.0 38.0 22.5 40.0 74-75 32.12409681205865 35.0 32.0 37.0 21.5 39.0 76-77 30.896968567755398 34.0 30.5 36.0 20.5 38.5 78-79 31.425368914311267 34.5 31.0 36.0 20.5 39.0 80-81 31.23328007676399 35.0 31.0 36.0 20.0 37.5 82-83 30.897521007620504 34.0 31.0 35.5 18.5 37.0 84-85 30.50887350710812 34.0 31.0 35.0 17.5 37.0 86-87 30.101014048555086 34.0 30.5 35.0 12.0 36.0 88-89 29.688172849397255 34.0 30.0 35.0 7.0 36.0 90-91 29.209987237055394 34.0 29.5 35.0 2.0 35.0 92-93 28.775578990516294 34.0 29.0 35.0 2.0 35.0 94-95 28.346601050846825 34.0 29.0 35.0 2.0 35.0 96-97 27.858879562519796 33.5 29.0 35.0 2.0 35.0 98-99 27.110936539285646 33.0 27.0 35.0 2.0 35.0 100-101 24.730779191742283 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2772498404009056 1101 2 0.2147419117310676 1101 3 0.09667061191660764 1101 4 0.1317997223695926 1101 5 -0.02560587668451575 1101 6 0.0853742053209885 1101 7 0.13257189562371252 1101 8 0.11044616012957675 1101 9 0.2125315746359817 1101 10-11 0.225006876434648 1101 12-13 0.15156275358442173 1101 14-15 0.24254145116692882 1101 16-17 0.14734892430720947 1101 18-19 0.31895003128840216 1101 20-21 0.2864017298269559 1101 22-23 0.21724791141041067 1101 24-25 0.20057237728970279 1101 26-27 0.19490631859482477 1101 28-29 0.11927487763451694 1101 30-31 0.16520458069410182 1101 32-33 0.19852823029995648 1101 34-35 0.05421003609473729 1101 36-37 0.2057457570986969 1101 38-39 0.2571950538943 1101 40-41 0.09535448678888514 1101 42-43 0.039454567675299757 1101 44-45 0.005458108900633363 1101 46-47 0.047408588945337726 1101 48-49 0.1878018771640697 1101 50-51 0.29957382794824383 1101 52-53 0.2181388426993891 1101 54-55 0.3080701269380768 1101 56-57 0.32250155791768265 1101 58-59 0.2822565706432343 1101 60-61 0.3520798956916309 1101 62-63 0.25585746726222425 1101 64-65 0.08122717787697553 1101 66-67 0.33003552349785537 1101 68-69 0.32338513415626124 1101 70-71 0.40329029681866047 1101 72-73 0.3098791076489462 1101 74-75 0.35777763731704937 1101 76-77 0.28919802854607113 1101 78-79 0.2922440847353478 1101 80-81 0.1965839519246444 1101 82-83 0.2598497929168708 1101 84-85 0.40682326376347433 1101 86-87 0.3914277797144585 1101 88-89 0.4868961483785412 1101 90-91 0.4545130806401616 1101 92-93 0.6749692192938603 1101 94-95 0.7768302343396876 1101 96-97 0.821555503754368 1101 98-99 1.138171416605502 1101 100-101 1.9838589008657301 1102 1 0.1580791864934099 1102 2 0.13973393724940308 1102 3 0.1999321749150127 1102 4 0.07827500147644884 1102 5 -0.0253108208631474 1102 6 0.0355416694358226 1102 7 0.029908418749705845 1102 8 0.06728826539273314 1102 9 0.17364800206819808 1102 10-11 0.09624292109174348 1102 12-13 0.19454919696560324 1102 14-15 0.14770094080009954 1102 16-17 0.18090155588615175 1102 18-19 0.17541733591360043 1102 20-21 0.12357078883811567 1102 22-23 0.119269442510884 1102 24-25 0.022713525615060348 1102 26-27 0.053498822582064065 1102 28-29 -0.01164617499706111 1102 30-31 0.08276837654737079 1102 32-33 0.11035837384062575 1102 34-35 0.1553145018044617 1102 36-37 0.166650860766957 1102 38-39 -0.03274912473408875 1102 40-41 0.06801397642205842 1102 42-43 0.07793542891932503 1102 44-45 0.1973360993312525 1102 46-47 0.2722331503488462 1102 48-49 0.2151822599391835 1102 50-51 0.36047893161650535 1102 52-53 0.25608940091311183 1102 54-55 0.08302626728895035 1102 56-57 0.30142101565292734 1102 58-59 0.3850835243912485 1102 60-61 0.33892598819561215 1102 62-63 0.3788279616800807 1102 64-65 0.4016099529965942 1102 66-67 0.35516311520439103 1102 68-69 0.3572886429281894 1102 70-71 0.4011092760850161 1102 72-73 0.35209601355006015 1102 74-75 0.4324985304590001 1102 76-77 0.3270050460899334 1102 78-79 0.5343294116890966 1102 80-81 0.5520225484158701 1102 82-83 0.4873657418801898 1102 84-85 0.5031988618495973 1102 86-87 0.6060928514847923 1102 88-89 0.6071912041999106 1102 90-91 0.7888551859033193 1102 92-93 0.9350011172204908 1102 94-95 0.6038437909585106 1102 96-97 0.841882457502372 1102 98-99 1.0249098536070917 1102 100-101 1.6796802724765776 1103 1 0.1424731834837374 1103 2 0.15891589052093025 1103 3 0.13120253654702196 1103 4 -0.008543698709225112 1103 5 -0.09131510289275724 1103 6 0.06614302284620521 1103 7 0.07126864645440634 1103 8 0.09586130965503514 1103 9 0.13247705703714274 1103 10-11 0.12777812138580202 1103 12-13 0.13097201361744482 1103 14-15 0.17401083973415155 1103 16-17 0.10184364167125892 1103 18-19 0.06412422894454295 1103 20-21 0.04010773273190438 1103 22-23 0.11837427683749269 1103 24-25 0.10608959808597973 1103 26-27 0.0625131153874392 1103 28-29 0.14066806094496798 1103 30-31 0.096674566169348 1103 32-33 0.16691717556349062 1103 34-35 0.3135090671140617 1103 36-37 0.2507396885338622 1103 38-39 0.23743461297824098 1103 40-41 0.18017790150067725 1103 42-43 0.2001517562385473 1103 44-45 0.24851487485299373 1103 46-47 0.409443846312179 1103 48-49 0.32519338913207463 1103 50-51 0.4204675982182451 1103 52-53 0.2754547463186441 1103 54-55 0.3116131739545338 1103 56-57 0.42747758211235265 1103 58-59 0.3842625795172765 1103 60-61 0.25310220015450824 1103 62-63 0.42449751183135476 1103 64-65 0.5487147920356321 1103 66-67 0.4450986962629315 1103 68-69 0.1535884808205381 1103 70-71 0.36136128907875786 1103 72-73 0.5524324811740868 1103 74-75 0.5766478894112055 1103 76-77 0.43713889485857393 1103 78-79 0.5156707346885909 1103 80-81 0.49697035038064286 1103 82-83 0.37152690547382505 1103 84-85 0.4035319129606343 1103 86-87 0.4668817282609865 1103 88-89 0.7539846300435151 1103 90-91 0.8425916337351289 1103 92-93 1.062609840874071 1103 94-95 1.0425030491589276 1103 96-97 1.2645117901906247 1103 98-99 1.620425739214383 1103 100-101 2.435300632694858 1104 1 0.09181433936466732 1104 2 0.15061151347867252 1104 3 0.07623595107529724 1104 4 0.04381226928047255 1104 5 -0.05787832389226821 1104 6 0.011146904587221229 1104 7 0.04442536562875432 1104 8 0.022542726532982726 1104 9 0.03878934643903875 1104 10-11 0.041169986276131 1104 12-13 0.09595361226363508 1104 14-15 0.08935935292013397 1104 16-17 0.05375961158665632 1104 18-19 0.09036491954247339 1104 20-21 0.10887806433655811 1104 22-23 0.0911385740441375 1104 24-25 -0.024284509508028407 1104 26-27 0.07640242569166844 1104 28-29 0.05625465694331666 1104 30-31 0.08369166969875863 1104 32-33 0.1160166076034912 1104 34-35 0.1920787416344254 1104 36-37 0.21809382184971327 1104 38-39 0.054238688017413494 1104 40-41 -0.029841278628694567 1104 42-43 0.11670544987997289 1104 44-45 -0.11094081564986169 1104 46-47 0.03741082946513785 1104 48-49 0.15286209113370575 1104 50-51 0.2435328264358887 1104 52-53 0.24249777397144356 1104 54-55 0.1374091735969003 1104 56-57 0.35567973525482444 1104 58-59 0.13447972641538541 1104 60-61 0.23913865332339412 1104 62-63 0.23085102576779803 1104 64-65 0.2770105196122614 1104 66-67 0.2112218787322675 1104 68-69 0.20465046771364115 1104 70-71 0.3927447302472231 1104 72-73 0.4463452477934595 1104 74-75 0.49895278995246173 1104 76-77 0.10755460727233768 1104 78-79 0.29431096425139813 1104 80-81 0.29791370042629595 1104 82-83 0.42378638620651543 1104 84-85 0.4958760840177696 1104 86-87 0.7085218255330865 1104 88-89 0.664216486697029 1104 90-91 0.7057862560168502 1104 92-93 0.9951848693246639 1104 94-95 0.8261300719020035 1104 96-97 1.0507427551967012 1104 98-99 1.1555096790823924 1104 100-101 1.62098823487122 1105 1 0.19252408400978993 1105 2 0.1607347565198438 1105 3 0.0918235486332648 1105 4 0.08673705503952789 1105 5 -0.05254548385867963 1105 6 0.05544030810014533 1105 7 0.09608695236785536 1105 8 0.10811337172955149 1105 9 0.24163622552327269 1105 10-11 0.2086527171719652 1105 12-13 0.1067476755168002 1105 14-15 0.21420634087716195 1105 16-17 0.16273381306973533 1105 18-19 0.3010935145968574 1105 20-21 0.2519460708130481 1105 22-23 0.23463436389459247 1105 24-25 0.17004355505642366 1105 26-27 0.1156457693968207 1105 28-29 0.07831247803255792 1105 30-31 0.2442850649739583 1105 32-33 0.1658791436555589 1105 34-35 0.06056040451704092 1105 36-37 0.08317248726194038 1105 38-39 -0.049619455547265545 1105 40-41 -0.10021212538445212 1105 42-43 0.03772594446113686 1105 44-45 0.09788756373040286 1105 46-47 0.14926823308019976 1105 48-49 0.16559418649664082 1105 50-51 0.20231507939941906 1105 52-53 0.28217371843090433 1105 54-55 0.24176856558569426 1105 56-57 0.3107208106482702 1105 58-59 0.13049406679316888 1105 60-61 0.2597146482208288 1105 62-63 0.3523969038485717 1105 64-65 0.29590910267617687 1105 66-67 0.24079866705223907 1105 68-69 0.18674595108235792 1105 70-71 0.3023916307206278 1105 72-73 0.2434865661273946 1105 74-75 0.2994484502153867 1105 76-77 0.2881266422831317 1105 78-79 0.43109782937485974 1105 80-81 0.3716913985567203 1105 82-83 0.32067951310546405 1105 84-85 0.3119181532367854 1105 86-87 0.3407020707088435 1105 88-89 0.4563869911297438 1105 90-91 0.39427710082638967 1105 92-93 0.660632497896934 1105 94-95 0.6285100504839285 1105 96-97 0.8512287806989498 1105 98-99 1.2010226024277557 1105 100-101 1.4433497436169311 1106 1 -0.17957471868780317 1106 2 -0.015617414477318903 1106 3 -0.05373533433839128 1106 4 0.027768407772235548 1106 5 -0.14007304473658877 1106 6 -0.11870812744059123 1106 7 -0.13455745301506994 1106 8 -0.14877273418365888 1106 9 -0.08270438437356376 1106 10-11 -0.08975144700292503 1106 12-13 -0.16729681452043366 1106 14-15 -0.01712517151856474 1106 16-17 0.09628018172902841 1106 18-19 0.09904547044045131 1106 20-21 -0.11668882741454212 1106 22-23 -0.05666553363511184 1106 24-25 -0.2016455934188386 1106 26-27 -0.3314537992747333 1106 28-29 -0.10619022330228489 1106 30-31 -0.17014089144382183 1106 32-33 -0.07425709757971077 1106 34-35 -0.31020787304014874 1106 36-37 -0.045648446182305236 1106 38-39 0.1944550266259668 1106 40-41 0.11052887215195284 1106 42-43 0.033699190976527404 1106 44-45 0.12803232713523016 1106 46-47 0.33340894079474737 1106 48-49 0.35932318604386637 1106 50-51 0.2347015932646812 1106 52-53 0.4902292679973641 1106 54-55 0.3131284019963516 1106 56-57 0.28028270758562 1106 58-59 0.29518527715685394 1106 60-61 0.3409368249259188 1106 62-63 0.5381172200880115 1106 64-65 0.2199341313009029 1106 66-67 0.6122750449826526 1106 68-69 0.08886298963411576 1106 70-71 0.2538277467900727 1106 72-73 0.48765409995224474 1106 74-75 0.4086462655441707 1106 76-77 0.10058387540361835 1106 78-79 0.3232991931489444 1106 80-81 0.47472794629505444 1106 82-83 0.40852991782341164 1106 84-85 0.4017407990583699 1106 86-87 0.5309501441424835 1106 88-89 0.60741776512657 1106 90-91 0.4590836044559694 1106 92-93 0.6505812173586882 1106 94-95 0.6641658546941791 1106 96-97 0.9150911504975596 1106 98-99 0.8389745128802915 1106 100-101 2.0312455393844466 1107 1 -0.009262158926624409 1107 2 -0.00458814332714752 1107 3 0.017680152537799643 1107 4 0.09077757299792921 1107 5 -0.19426724767598103 1107 6 -0.05103482254435221 1107 7 -0.05921056140146419 1107 8 -0.010368365150966952 1107 9 -0.02524508236397338 1107 10-11 0.0809838444504507 1107 12-13 0.006541528854448586 1107 14-15 -0.011422319690048255 1107 16-17 -0.010758502246829948 1107 18-19 0.04774295357646707 1107 20-21 0.053067135618576344 1107 22-23 -0.02832137367344245 1107 24-25 0.10436887205707279 1107 26-27 -0.040967677958782645 1107 28-29 0.07782991483033186 1107 30-31 -0.12207529153722163 1107 32-33 -0.15472608819708 1107 34-35 0.12265327570302986 1107 36-37 -0.007743504448818328 1107 38-39 -0.04328323804030987 1107 40-41 0.13634469399268312 1107 42-43 0.2508806173537792 1107 44-45 -0.04729564220263427 1107 46-47 0.07112606887640993 1107 48-49 0.11611356547575724 1107 50-51 -0.011273900375215362 1107 52-53 0.010022169728344466 1107 54-55 -0.1451560320162386 1107 56-57 0.09290587851617715 1107 58-59 0.041153725268550545 1107 60-61 0.02603694188168504 1107 62-63 -0.2551810734850548 1107 64-65 -0.11381253968701799 1107 66-67 -0.25024355017927746 1107 68-69 0.014980561382749613 1107 70-71 0.03074921330670577 1107 72-73 -0.021006867217607805 1107 74-75 0.009456702588352073 1107 76-77 0.0644352832008046 1107 78-79 0.11460360224049637 1107 80-81 0.1985694098324302 1107 82-83 0.112687182064942 1107 84-85 -0.0019246873145739585 1107 86-87 0.09589884263212056 1107 88-89 0.33200605465349753 1107 90-91 0.6015260676531504 1107 92-93 0.6812151739864127 1107 94-95 0.7069000027589745 1107 96-97 1.2300403522392145 1107 98-99 1.4779032705121082 1107 100-101 2.0117566018702284 1108 1 0.019149287211504884 1108 2 0.13933778973567001 1108 3 0.11799630829884933 1108 4 0.12075747436449547 1108 5 -0.07286881299430092 1108 6 -0.011048176820295907 1108 7 0.053308176036892974 1108 8 0.04552913854050189 1108 9 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 8.0 5 19.0 6 36.0 7 108.0 8 195.0 9 454.0 10 779.0 11 1284.0 12 2001.0 13 2954.0 14 4025.0 15 5584.0 16 7978.0 17 11977.0 18 10849.0 19 6019.0 20 4811.0 21 4841.0 22 5006.0 23 5221.0 24 6173.0 25 7199.0 26 8543.0 27 10499.0 28 13148.0 29 16413.0 30 20542.0 31 26442.0 32 34785.0 33 46706.0 34 65781.0 35 98042.0 36 158905.0 37 254810.0 38 212597.0 39 18680.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.061940073147 20.209561304417527 15.239517274547303 21.48898134788817 2 15.407855266344953 21.366939315459 40.12026979188016 23.104935626315886 3 19.38980082353599 25.68985113003298 30.199176464012222 24.721171582418812 4 12.80440088688491 17.56404762348382 37.25317210411582 32.37837938551545 5 13.337886604902438 38.432158893648186 33.84587144679428 14.3840830546551 6 31.002589852993236 37.42728847981219 17.06694490506978 14.503176762124797 7 27.938365225168155 34.26394142087906 20.323358983436123 17.47433437051667 8 26.52549794116003 35.569488177973206 19.368280822045424 18.536733058821337 9 26.759143671628998 17.35117661306851 20.531758305230014 35.35792141007248 10-11 24.926589778465093 27.771981144379648 26.656714054144697 20.644715023010566 12-13 25.803668710221828 25.178956645606913 27.819693871989692 21.197680772181567 14-15 22.623018017178737 26.48082763503568 26.543664176184535 24.35249017160105 16-17 21.372063125337707 29.95011272381733 27.1354176370852 21.542406513759758 18-19 21.33731437834212 27.991466527547466 30.246967636153606 20.42425145795681 20-21 22.23435895166606 27.075532120540235 30.47039013245079 20.21971879534291 22-23 21.91711143013386 26.97581648138608 30.31361530514236 20.793456783337696 24-25 21.799940097641777 27.349758504062045 30.37864718489194 20.471654213404246 26-27 21.54553686717086 27.506349020024334 30.390238812588855 20.55787530021595 28-29 21.141918591299593 27.592484161110654 30.589959907265463 20.67563734032429 30-31 21.32024549707755 27.33367554363927 30.58633481583061 20.759744143452572 32-33 21.222354716699893 27.543831864507833 30.377811108419817 20.856002310372453 34-35 21.751225056361907 27.415643457360584 30.166384080788504 20.666747405488998 36-37 21.75674177557878 27.30406299869855 30.027603386934643 20.91159183878802 38-39 21.524659499543517 27.602662517933336 30.115891263438353 20.756786719084793 40-41 21.53229863427177 27.538894374988352 30.137085204300273 20.791721786439606 42-43 21.552424116644936 27.691898461625897 29.72733375352939 21.028343668199774 44-45 21.625784874513236 27.932682454211772 29.354772596001567 21.086760075273425 46-47 21.773257438840343 28.220407668945985 28.769912988392242 21.23642190382143 48-49 21.98952879581152 28.352508803636972 28.56635799593822 21.091604404613292 50-51 22.025674945501294 28.508272623949622 28.314406290175327 21.15164614037376 52-53 22.097930958027128 28.717616924231393 28.00051610763546 21.183936010106024 54-55 21.696120810120924 28.827159918764323 28.186404203387305 21.290315067727448 56-57 21.82831510499152 29.125924614782654 28.007070857632616 21.038689422593208 58-59 21.848251383428668 28.51088110897878 28.277421698869038 21.36344580872352 60-61 21.831156490469713 28.50333513443014 28.711222075236158 20.954286299863988 62-63 21.75537068435468 28.5974734959289 28.6504816381286 20.99667418158782 64-65 21.705390247992398 28.42913305136852 28.555085614205066 21.310391086434013 66-67 21.508915429188946 28.968670231596207 28.20023849005981 21.322175849155037 68-69 21.56611577947122 28.70344320023849 28.372771142702764 21.357669877587522 70-71 21.60030556538913 28.915568929216896 28.29852247955134 21.185603025842635 72-73 21.75931381842217 28.705113615984818 28.206845699044685 21.328726866548323 74-75 21.64808040852659 29.171194645890676 27.801738091899853 21.378986853682882 76-77 21.78275977716085 28.804149354399954 28.001853887574295 21.4112369808649 78-79 21.81224497400831 28.86954780048816 27.896396564252573 21.421810661250955 80-81 21.52913118816493 29.25248271878668 27.814508766372903 21.403877326675484 82-83 21.585842960304785 29.09611506810264 27.7646654397431 21.553376531849473 84-85 21.49109527593605 28.90478927613541 27.772426238030068 21.831689209898464 86-87 21.488979150751803 28.800516107395058 27.970132846416128 21.740371895437015 88-89 21.526196642507127 28.674563544558513 27.921223752119396 21.878016060814964 90-91 21.36374647126233 28.996161478762357 27.911127053189606 21.728964996785706 92-93 21.34779279144991 28.986660977536634 27.908816164085444 21.756730066928018 94-95 21.194173762367015 28.9519479793557 27.89360175886419 21.96027649941309 96-97 21.30005030649699 29.127275437387045 27.700154645898156 21.872519610217807 98-99 21.231347541846485 29.469847473140693 27.268001499882384 22.030803485130438 100-101 21.042078566783257 29.29627738572858 27.525720270051192 22.13592377743697 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 432.0 1 298.5 2 204.5 3 269.5 4 257.5 5 242.0 6 408.5 7 857.5 8 1339.5 9 1609.5 10 1638.0 11 1641.5 12 1805.0 13 1997.5 14 2126.5 15 2270.5 16 2445.5 17 2464.0 18 2515.0 19 2683.5 20 2817.5 21 3120.5 22 3646.5 23 4239.5 24 5012.5 25 6090.0 26 7519.5 27 9249.0 28 10577.5 29 12258.0 30 14481.0 31 16537.5 32 18774.5 33 21443.5 34 24931.0 35 27198.0 36 29683.5 37 32703.0 38 33988.5 39 34225.0 40 34143.0 41 35273.5 42 37052.5 43 38365.0 44 39710.0 45 40224.5 46 39248.5 47 39487.5 48 42462.5 49 42116.0 50 39125.0 51 37039.0 52 34660.5 53 32551.0 54 30342.0 55 27859.5 56 24564.5 57 21170.0 58 18564.5 59 16396.0 60 14092.0 61 11363.5 62 8726.0 63 6501.0 64 4557.5 65 3321.5 66 2353.0 67 1738.5 68 1318.5 69 839.0 70 614.0 71 491.0 72 406.0 73 326.0 74 233.5 75 163.0 76 92.5 77 53.5 78 35.5 79 23.5 80 15.0 81 6.0 82 5.0 83 3.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04769801196176707 2 0.0 3 0.0 4 0.0 5 0.018538875742952432 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004238788172383596 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.3290044903206576E-4 22-23 2.7948053883847886E-4 24-25 6.055411674833709E-4 26-27 0.0024221646699334838 28-29 0.003120866017029681 30-31 5.589610776769577E-4 32-33 0.0 34-35 0.0 36-37 9.31601796128263E-5 38-39 0.0 40-41 0.0 42-43 0.010387360026830132 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 4.658008980641315E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.3974026941923943E-4 74-75 1.3974026941923943E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.2606062864489203E-4 84-85 9.31601796128263E-5 86-87 0.0 88-89 0.0 90-91 0.008384416165154366 92-93 0.0026084850291591363 94-95 0.0 96-97 0.0 98-99 2.3290044903206576E-4 100-101 0.013228745505021334 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1073420.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.66783433049416 #Duplication Level Percentage of deduplicated Percentage of total 1 76.01961993068936 42.31847608168747 2 13.319573471570898 14.829436187365076 3 4.6034933960785684 7.687995231432771 4 2.027501970500888 4.514665751943756 5 1.113304737833673 3.098763185253957 6 0.6666829755396859 2.2267678459982476 7 0.4491515520243245 1.7502305931162094 8 0.2895309977993518 1.289405089522959 9 0.226232506530107 1.1334486324321391 >10 1.1535285856167934 11.696577647414042 >50 0.0855200581564581 3.2668846086728722 >100 0.04383659111994819 4.15355748127742 >500 0.0010116132699702395 0.367263223073263 >1k 8.430110583085329E-4 1.1123016756047124 >5k 1.686022116617066E-4 0.5542267652051157 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5905 0.5501108606137393 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4947 0.46086340854465163 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3408 0.31748989212051204 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1232 0.11477334128300198 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1155 0.10760000745281438 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 1109 0.10331463919062435 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 9.316017961282629E-5 0.0 16-17 0.0 0.0 0.0 9.316017961282629E-5 0.0 18-19 0.0 0.0 0.0 9.316017961282629E-5 0.0 20-21 0.0 0.0 0.0 9.316017961282629E-5 0.0 22-23 0.0 0.0 0.0 3.2606062864489203E-4 0.0 24-25 0.0 0.0 0.0 7.452814369026104E-4 0.0 26-27 0.0 0.0 0.0 0.001304242514579568 0.0 28-29 0.0 0.0 0.0 0.0023290044903206573 0.0 30-31 0.0 0.0 0.0 0.005123809878705446 0.0 32-33 0.0 0.0 0.0 0.008058355536509474 0.0 34-35 0.0 0.0 0.0 0.011179221553539155 0.0 36-37 0.0 0.0 0.0 0.015278269456503513 0.0 38-39 0.0 0.0 0.0 0.021520001490562872 0.0 40-41 0.0 0.0 0.0 0.028320694602299194 0.0 42-43 0.0 0.0 0.0 0.040012297143708894 0.0 44-45 0.0 0.0 0.0 0.051284678876860876 0.0 46-47 0.0 0.0 0.0 0.07373628216355202 0.0 48-49 0.0 0.0 0.0 0.09949507182649847 0.0 50-51 0.0 0.0 0.0 0.11258407706210058 0.0 52-53 0.0 0.0 0.0 0.13382459801382496 0.0 54-55 0.0 0.0 0.0 0.1668498816865719 0.0 56-57 0.0 0.0 0.0 0.22623949618974865 0.0 58-59 0.0 0.0 0.0 0.29801941458143133 0.0 60-61 0.0 0.0 0.0 0.3804661735387826 0.0 62-63 0.0 0.0 0.0 0.47381267351083456 0.0 64-65 0.0 0.0 0.0 0.5717706023737215 0.0 66-67 0.0 0.0 0.0 0.7036854167054833 0.0 68-69 0.0 0.0 0.0 0.8599150379161931 0.0 70-71 0.0 0.0 0.0 0.9588045685752082 0.0 72-73 0.0 0.0 0.0 1.0916509847030986 0.0 74-75 0.0 0.0 0.0 1.2469024240278737 0.0 76-77 0.0 0.0 0.0 1.4198542974790855 0.0 78-79 0.0 0.0 0.0 1.6102737046077025 0.0 80-81 0.0 0.0 0.0 1.8973467980846266 0.0 82-83 0.0 0.0 0.0 2.197508896797153 0.0 84-85 0.0 0.0 0.0 2.5974921279648227 0.0 86-87 0.0 0.0 0.0 3.0697210784222393 0.0 88-89 0.0 0.0 0.0 3.6538819846844666 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4525 0.0 32.7706 1 GTATCAA 6815 0.0 32.568623 1 ATCAACG 8505 0.0 26.02658 3 TCAACGC 8505 0.0 26.02658 4 TATCAAC 8520 0.0 25.98076 2 CAACGCA 8655 0.0 25.520632 5 AACGCAG 8855 0.0 24.995535 6 GTACATG 5640 0.0 24.016743 1 GCACCGT 180 1.9520121E-7 23.748438 6 TACATGG 5840 0.0 22.937273 2 GAGTACT 5215 0.0 22.680393 12-13 ACATGGG 5715 0.0 22.025976 3 ACGCAGA 10035 0.0 21.963732 7 TGCGCCG 130 2.940787E-4 21.92368 5 CGCAGAG 10025 0.0 21.84349 8 AGTACTT 5390 0.0 21.679632 12-13 GCGAACT 55 0.002879268 21.58949 66-67 GTACTTT 5515 0.0 21.487709 14-15 GCAGAGT 10445 0.0 20.601332 9 TGCACCG 165 6.3208834E-5 20.15207 5 >>END_MODULE