FastQCFastQC Report
Thu 26 May 2016
SRR937815_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937815_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150619
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104980.9123784675900536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79540.6912800848934356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57420.49903573641665916No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24890.21631834690718646No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18050.15687208363498256No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15860.1378388502188822No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15010.13045152218066972No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13350.11602450507074888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57200.038.0838971
GTATCAA89350.034.81411
TATCAAC112600.027.3132172
GGTATAG1056.8435096E-527.1790241
TCAACGC113200.027.128824
ATCAACG113300.027.1025143
CAACGCA114850.026.739075
AACGCAG117750.026.1027056
TATAGGG2950.025.7813072
GAGTACT68600.024.51226812-13
GTACATG67150.023.7285231
ACGCAGA130650.023.489047
CGCAGAG131650.023.3096058
GTACTTT72650.022.9813314-15
TACATGG68350.022.8108922
ACATGGG66250.022.6012063
AGTACTT72450.022.45569612-13
GCAGAGT133200.022.326079
CATGGGA38300.022.0935764
GTGTAAG3351.8189894E-1221.2969971
Produced by FastQC (version 0.11.5)