##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937815_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1150619 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.837604802284684 33.0 31.0 34.0 30.0 34.0 2 31.96163804004627 33.0 31.0 34.0 30.0 34.0 3 31.969414723726967 34.0 31.0 34.0 30.0 34.0 4 35.57986961800561 37.0 35.0 37.0 33.0 37.0 5 35.57447947582997 37.0 35.0 37.0 33.0 37.0 6 35.657771164912106 37.0 35.0 37.0 33.0 37.0 7 35.60810833125474 37.0 35.0 37.0 33.0 37.0 8 35.60120074499031 37.0 35.0 37.0 33.0 37.0 9 37.24486211334942 39.0 37.0 39.0 34.0 39.0 10-11 37.12981447377455 39.0 37.0 39.0 34.0 39.0 12-13 37.01671100511985 39.0 37.0 39.0 33.0 39.0 14-15 38.13440026629145 40.0 38.0 41.0 33.0 41.0 16-17 38.070001016843975 40.0 38.0 41.0 33.0 41.0 18-19 38.06422542996422 40.0 38.0 41.0 33.0 41.0 20-21 37.98945046101272 40.0 38.0 41.0 33.0 41.0 22-23 37.902877060086794 40.0 38.0 41.0 33.0 41.0 24-25 37.91779294449336 40.0 38.0 41.0 33.0 41.0 26-27 37.80146295168079 40.0 37.5 41.0 33.0 41.0 28-29 37.67325804632115 40.0 37.0 41.0 33.0 41.0 30-31 37.383623945024375 39.5 37.0 41.0 32.0 41.0 32-33 37.165709500712225 39.0 36.0 41.0 32.0 41.0 34-35 37.12670701596271 40.0 36.0 41.0 32.0 41.0 36-37 36.961907460245314 39.5 36.0 41.0 31.0 41.0 38-39 36.786092963874225 39.0 35.0 41.0 31.0 41.0 40-41 36.52401185796515 39.0 35.0 41.0 30.5 41.0 42-43 36.20049947028512 39.0 35.0 41.0 30.0 41.0 44-45 35.88640592585382 39.0 35.0 41.0 29.5 41.0 46-47 35.42175168322442 39.0 35.0 40.0 26.5 41.0 48-49 35.00045801433837 39.0 35.0 40.0 25.0 41.0 50-51 34.049551154639374 37.5 34.0 39.5 23.5 40.5 52-53 34.09409109357659 38.0 33.5 39.5 23.0 40.5 54-55 34.35909410499913 38.0 34.0 40.0 23.0 41.0 56-57 34.10569658592462 38.0 34.0 40.0 22.5 41.0 58-59 33.841529646216514 38.0 34.0 40.0 21.5 41.0 60-61 33.50121195634698 37.0 33.0 40.0 20.0 41.0 62-63 33.09921311919932 36.5 33.0 40.0 18.5 41.0 64-65 32.678635152035554 36.0 32.5 39.0 15.5 41.0 66-67 32.240715649576444 35.5 32.0 39.0 11.0 40.5 68-69 31.723967273267693 35.0 31.0 38.5 7.5 40.0 70-71 31.263718919990023 35.0 31.0 38.0 4.5 40.0 72-73 30.804016794438475 35.0 30.5 37.0 2.0 39.5 74-75 30.29184117418537 35.0 30.0 36.5 2.0 39.0 76-77 29.83207734271727 34.0 29.5 36.0 2.0 39.0 78-79 29.292024553740205 34.0 29.0 35.5 2.0 37.5 80-81 28.757207207598693 34.0 28.5 35.0 2.0 37.0 82-83 28.280990492943364 34.0 27.5 35.0 2.0 36.5 84-85 27.793163505904214 33.5 26.5 35.0 2.0 36.0 86-87 27.287597371501775 33.0 26.0 35.0 2.0 36.0 88-89 26.822038398462045 33.0 25.0 35.0 2.0 35.0 90-91 26.28935251373391 32.5 24.5 35.0 2.0 35.0 92-93 25.690924624050183 32.0 22.0 35.0 2.0 35.0 94-95 24.985713776671513 32.0 19.0 34.0 2.0 35.0 96-97 24.185085593059043 31.0 8.5 34.0 2.0 35.0 98-99 23.176135193317684 31.0 2.0 34.0 2.0 35.0 100-101 20.410411700137058 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3114066819886716 1101 2 0.43180014532036637 1101 3 0.2891606382024534 1101 4 0.29785098672552834 1101 5 0.20171624547936062 1101 6 0.27285057624203546 1101 7 0.27624420441281927 1101 8 0.3089578932280759 1101 9 0.4044310360667751 1101 10-11 0.4611707644464218 1101 12-13 0.3733380324343969 1101 14-15 0.4781850645800674 1101 16-17 0.5741778930606074 1101 18-19 0.5366740053562111 1101 20-21 0.5720688134859202 1101 22-23 0.5440797733500204 1101 24-25 0.521015984428729 1101 26-27 0.5872896561098244 1101 28-29 0.6434816526478429 1101 30-31 0.5590304796928152 1101 32-33 0.47205738408742803 1101 34-35 0.37504772227848804 1101 36-37 0.43657976751696026 1101 38-39 0.4667000691719778 1101 40-41 0.44013267030480563 1101 42-43 0.4136797403966952 1101 44-45 0.3540808685870047 1101 46-47 0.26889170263363127 1101 48-49 0.4303614853775386 1101 50-51 0.48199899670999713 1101 52-53 0.3704106742573643 1101 54-55 0.43540546991155793 1101 56-57 0.3167229902616455 1101 58-59 0.3912094358314633 1101 60-61 0.24181788965179862 1101 62-63 0.29061200398526665 1101 64-65 0.4021802041109268 1101 66-67 0.5489970502344548 1101 68-69 0.5294625589408959 1101 70-71 0.6295361476778751 1101 72-73 0.6272098310859029 1101 74-75 0.7632723960700432 1101 76-77 0.5304083967984532 1101 78-79 0.6565706590374241 1101 80-81 0.6614231602786482 1101 82-83 0.6775250861310802 1101 84-85 0.6677233202139199 1101 86-87 0.5942371711798735 1101 88-89 0.5023822694336033 1101 90-91 0.4901427850686737 1101 92-93 0.3934304913178117 1101 94-95 0.41139103762575857 1101 96-97 0.7805704526211024 1101 98-99 0.9866244389577616 1101 100-101 1.3652292786911637 1102 1 0.20771500074459937 1102 2 0.4332981410801082 1102 3 0.2800274995597789 1102 4 0.2704643793931609 1102 5 0.19667331838588353 1102 6 0.13044826923097474 1102 7 0.25598588553489776 1102 8 0.12593830949523976 1102 9 0.4814704694632965 1102 10-11 0.24957337398431179 1102 12-13 0.46528606125252026 1102 14-15 0.4892083859831118 1102 16-17 0.4959057848145534 1102 18-19 0.4440800573566719 1102 20-21 0.4530281651026016 1102 22-23 0.3791803879186375 1102 24-25 0.4686867718036041 1102 26-27 0.37962938398205637 1102 28-29 0.3062868326879027 1102 30-31 0.4801978429031095 1102 32-33 0.6054674470081309 1102 34-35 0.5633984265072698 1102 36-37 0.737952909965621 1102 38-39 0.6128174788591778 1102 40-41 0.6679265299860262 1102 42-43 0.9411273410417635 1102 44-45 0.9704314069139812 1102 46-47 0.8802163919946864 1102 48-49 1.069828489215233 1102 50-51 0.9145699746279803 1102 52-53 0.8787612686981916 1102 54-55 0.6741503739202841 1102 56-57 0.8932162285429897 1102 58-59 0.9855118065195327 1102 60-61 0.7738555420435915 1102 62-63 0.8321685777225198 1102 64-65 0.8762409680887657 1102 66-67 0.7549196228835982 1102 68-69 0.6669594787786117 1102 70-71 0.641758292641903 1102 72-73 0.7568678903762844 1102 74-75 0.7316427889401709 1102 76-77 0.8223812498948391 1102 78-79 0.7037337380774424 1102 80-81 0.8500083837947443 1102 82-83 0.6689155750778717 1102 84-85 0.6525454393167784 1102 86-87 0.9226346206835068 1102 88-89 0.9958514104769804 1102 90-91 0.9180816258541675 1102 92-93 0.893712585389018 1102 94-95 1.141581629529263 1102 96-97 0.9921504599890945 1102 98-99 1.158048584014498 1102 100-101 1.439672836699465 1103 1 0.2743222240755756 1103 2 0.2619442493656976 1103 3 0.22259751179179688 1103 4 0.2330221596030313 1103 5 0.21994248725216892 1103 6 0.20651599600623172 1103 7 0.23925634198246115 1103 8 0.30844587403525026 1103 9 0.35250117827678906 1103 10-11 0.41788020460299435 1103 12-13 0.4840197250650462 1103 14-15 0.44724546832393486 1103 16-17 0.48128208044321497 1103 18-19 0.35099823156642174 1103 20-21 0.24368805050787756 1103 22-23 0.2547153779398883 1103 24-25 0.28943936306092866 1103 26-27 0.3263492706749105 1103 28-29 0.22273761489352495 1103 30-31 0.32599324623794956 1103 32-33 0.42345817642291905 1103 34-35 0.5186346976718923 1103 36-37 0.4472605131330738 1103 38-39 0.5184821926942433 1103 40-41 0.6186730052322815 1103 42-43 0.5399047826177608 1103 44-45 0.5344142177039117 1103 46-47 0.793115703138703 1103 48-49 0.873329744606977 1103 50-51 0.7293459354855045 1103 52-53 0.7680930140146174 1103 54-55 0.8066721018543603 1103 56-57 0.7896390058558111 1103 58-59 0.7473768680015453 1103 60-61 0.8250314256462445 1103 62-63 0.8820462575010524 1103 64-65 0.950471316379506 1103 66-67 1.0188898797560597 1103 68-69 1.1911289888539365 1103 70-71 1.1198330367351303 1103 72-73 1.0656469414288843 1103 74-75 0.9205872941870652 1103 76-77 1.145218757137485 1103 78-79 1.098745004413626 1103 80-81 1.2159724602770687 1103 82-83 1.4301178540967356 1103 84-85 1.1465250311535122 1103 86-87 0.9713879201105335 1103 88-89 1.215260088774798 1103 90-91 1.2450847804283 1103 92-93 0.7954492106384876 1103 94-95 1.2006140121984181 1103 96-97 1.2884161523859277 1103 98-99 1.4928081282107257 1103 100-101 2.0106765063061047 1104 1 0.16025914429820887 1104 2 0.221968986533291 1104 3 0.2020903998561181 1104 4 -0.006087302363567915 1104 5 0.18545887562569874 1104 6 0.1883094406568162 1104 7 0.11534786950256404 1104 8 0.15015273859926026 1104 9 0.20365764086182736 1104 10-11 0.19633413162834756 1104 12-13 0.3079158289611499 1104 14-15 0.30107354500915307 1104 16-17 0.2990309981921371 1104 18-19 0.17156718642108615 1104 20-21 0.28513517481213313 1104 22-23 0.2631012777543589 1104 24-25 0.37169044531201223 1104 26-27 0.32319528180664037 1104 28-29 0.29786439287745026 1104 30-31 0.32001922026392293 1104 32-33 0.2718193390698005 1104 34-35 0.22380476569910002 1104 36-37 0.3105876621745054 1104 38-39 0.33891509312714163 1104 40-41 0.46339780424279553 1104 42-43 0.4790266131681662 1104 44-45 0.5387555906167165 1104 46-47 0.4439753197125995 1104 48-49 0.4789327130670884 1104 50-51 0.3105704252814263 1104 52-53 0.38574298309315935 1104 54-55 0.49396956629468036 1104 56-57 0.5880681957135749 1104 58-59 0.6163317226706866 1104 60-61 0.5762620997340591 1104 62-63 0.6297889909580761 1104 64-65 0.6643983417351009 1104 66-67 0.675599836466013 1104 68-69 0.4463547155082317 1104 70-71 0.3571614226349382 1104 72-73 0.5158881288129287 1104 74-75 0.5995607017318996 1104 76-77 0.528558274762716 1104 78-79 0.5077493334179586 1104 80-81 0.737939065100818 1104 82-83 0.752554465104776 1104 84-85 0.7013549631263025 1104 86-87 0.7952345496714486 1104 88-89 0.9271339292200658 1104 90-91 0.9170328323763535 1104 92-93 0.8812871821907464 1104 94-95 1.0163839565397907 1104 96-97 0.9706177603018205 1104 98-99 1.1193511090394175 1104 100-101 1.0871143541725274 1105 1 0.2246022430599126 1105 2 0.21163575387447509 1105 3 0.13843994178705543 1105 4 0.12689971062344085 1105 5 0.07516792626498159 1105 6 0.017356052959243584 1105 7 0.13444381231943225 1105 8 0.13682745922746875 1105 9 0.2616421369858557 1105 10-11 0.15199505984742245 1105 12-13 0.12461773170957002 1105 14-15 0.20047755185502325 1105 16-17 0.47995317678872595 1105 18-19 0.37257393161387853 1105 20-21 0.30608956925490816 1105 22-23 0.4663812535924734 1105 24-25 0.3044559743566069 1105 26-27 0.22912098401140923 1105 28-29 0.3250726884578441 1105 30-31 0.4374218176665181 1105 32-33 0.2631734113541029 1105 34-35 0.24422558518968884 1105 36-37 0.2876781685673393 1105 38-39 0.41376458472081623 1105 40-41 0.4431722933196127 1105 42-43 0.5843281333534023 1105 44-45 0.6797583088287382 1105 46-47 0.4815732256826024 1105 48-49 0.5272927251480368 1105 50-51 0.5117664385704614 1105 52-53 0.5394266637535807 1105 54-55 0.5671941711471931 1105 56-57 0.4946603632359725 1105 58-59 0.4900536596484315 1105 60-61 0.5619844394098834 1105 62-63 0.7084439793805046 1105 64-65 0.4601438365503654 1105 66-67 0.5836739329122054 1105 68-69 0.3821304126792775 1105 70-71 0.30590802486991464 1105 72-73 0.5453716685290253 1105 74-75 0.28566560458099133 1105 76-77 0.31968482263345166 1105 78-79 0.33097091803488965 1105 80-81 0.37358992586795736 1105 82-83 0.3104785565172392 1105 84-85 0.39196404396794193 1105 86-87 0.47373248584380434 1105 88-89 0.7643251101321766 1105 90-91 0.8474103618236484 1105 92-93 0.8488901218867078 1105 94-95 0.7933335186056318 1105 96-97 0.8157441068235158 1105 98-99 0.8543173830754611 1105 100-101 1.2429403886032127 1106 1 0.10785254615701234 1106 2 0.12586919375102923 1106 3 0.1816825296852116 1106 4 0.1521603741305526 1106 5 0.1627541711674425 1106 6 0.18857615656334303 1106 7 0.1361158061472736 1106 8 0.3928960142809217 1106 9 0.3294080358065301 1106 10-11 0.07540462669410886 1106 12-13 0.10051600130066873 1106 14-15 0.1487741461457759 1106 16-17 0.31157998159419265 1106 18-19 0.22628549339057713 1106 20-21 0.31905220564859604 1106 22-23 0.4106439010078873 1106 24-25 0.2813147862159724 1106 26-27 0.2507665257285865 1106 28-29 0.14590813428612392 1106 30-31 0.24005214942007314 1106 32-33 0.37940894535608294 1106 34-35 0.4632033213298783 1106 36-37 0.276194646027939 1106 38-39 0.1553406896475238 1106 40-41 0.4273285812067442 1106 42-43 0.28139525558420786 1106 44-45 0.2910991001877079 1106 46-47 0.25430440610804084 1106 48-49 0.3388699322460198 1106 50-51 0.39728335689957106 1106 52-53 0.3646647327495032 1106 54-55 0.32917863053218355 1106 56-57 0.40203333799625085 1106 58-59 0.26224532723357896 1106 60-61 0.5433847480178429 1106 62-63 0.7617251432733738 1106 64-65 0.5656144636858187 1106 66-67 0.42664208016228855 1106 68-69 0.29843868998557 1106 70-71 0.4519512299839903 1106 72-73 0.6443179927878155 1106 74-75 0.6454881550053244 1106 76-77 0.604104529455892 1106 78-79 0.5114972048198574 1106 80-81 0.2478024244902457 1106 82-83 0.19955493698676463 1106 84-85 0.32434638718407527 1106 86-87 0.48348725273458015 1106 88-89 0.5837459191258958 1106 90-91 0.5304876240301972 1106 92-93 0.6362316847655798 1106 94-95 0.5571668703084853 1106 96-97 0.6493035689639335 1106 98-99 0.6252619438602061 1106 100-101 0.8348785567945498 1107 1 0.24026900785237615 1107 2 0.24679226452112601 1107 3 0.2528575651802427 1107 4 0.24234274859812643 1107 5 0.11686059626792655 1107 6 0.18570655830709626 1107 7 0.25399930245779245 1107 8 0.29360991433491535 1107 9 0.2675816914947333 1107 10-11 0.38393893189137884 1107 12-13 0.4211686099354566 1107 14-15 0.4345041439460786 1107 16-17 0.5459746663383314 1107 18-19 0.5232289213106682 1107 20-21 0.47289066826383674 1107 22-23 0.4849285011638642 1107 24-25 0.27557558970347884 1107 26-27 0.33589460779710123 1107 28-29 0.45680460877969153 1107 30-31 0.615839539185508 1107 32-33 0.5544019463359504 1107 34-35 0.46896751434285733 1107 36-37 0.5362082441242038 1107 38-39 0.4767431932970183 1107 40-41 0.5038753646570626 1107 42-43 0.5325500939068419 1107 44-45 0.5283933789886817 1107 46-47 0.2692123190002391 1107 48-49 0.3973967384931427 1107 50-51 0.3749314861614721 1107 52-53 0.2211723216870567 1107 54-55 0.3503356685468617 1107 56-57 0.563568223112334 1107 58-59 0.5299653464308918 1107 60-61 0.5748428438844257 1107 62-63 0.5436431747489721 1107 64-65 0.43899968975416925 1107 66-67 0.6437391865673945 1107 68-69 0.8198381940549986 1107 70-71 0.6161057483514156 1107 72-73 0.7346028529073969 1107 74-75 0.7506484325664644 1107 76-77 0.8749841063151322 1107 78-79 1.1431512732502753 1107 80-81 1.1571401172132596 1107 82-83 0.9762037061590796 1107 84-85 0.6520180131564395 1107 86-87 0.8823489121022696 1107 88-89 0.9324670981608278 1107 90-91 1.0762662150274558 1107 92-93 1.0220551694777171 1107 94-95 1.3677908829467142 1107 96-97 1.6735151793384802 1107 98-99 1.4415276666590167 1107 100-101 1.4674899911937338 1108 1 0.18631140529022616 1108 2 0.1201591497836958 1108 3 0.29022475546035054 1108 4 0.11051416845477036 1108 5 0.20062603778081467 1108 6 0.3089895637465787 1108 7 0.3403726439249226 1108 8 0.21580756569986903 1108 9 0.26711145612286913 1108 10-11 0.19808920386535078 1108 12-13 0.49455328788777564 1108 14-15 0.5512508777166047 1108 16-17 0.3777536811316793 1108 18-19 0.4685812165676424 1108 20-21 0.5688466440657791 1108 22-23 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1.0402539417376673 1108 90-91 1.3006269491029983 1108 92-93 0.7255082221949181 1108 94-95 1.0562446982077631 1108 96-97 1.0777866957840097 1108 98-99 1.0975339703548137 1108 100-101 1.671138763498142 1109 1 0.12654863549830608 1109 2 0.23010070384259507 1109 3 0.2242885433891857 1109 4 0.10058266652332293 1109 5 0.1576603729761814 1109 6 0.10903551632925712 1109 7 0.21926461227508298 1109 8 0.023415590169619804 1109 9 0.27215188003544455 1109 10-11 0.3471687161935648 1109 12-13 0.2948591597662258 1109 14-15 0.5033015117186466 1109 16-17 0.5034002483301521 1109 18-19 0.48250269509199484 1109 20-21 0.4400846353635046 1109 22-23 0.28265145344279574 1109 24-25 0.21818106236281665 1109 26-27 0.25702962386505845 1109 28-29 0.11146180063457223 1109 30-31 0.19012741957354962 1109 32-33 0.3719829021654206 1109 34-35 0.18669142214952927 1109 36-37 0.1882641374671934 1109 38-39 0.22303863744138397 1109 40-41 0.2941992634113504 1109 42-43 0.22883183377195593 1109 44-45 0.23077918085246552 1109 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2316 88-89 -1.6101004168732445 2316 90-91 -1.7714141564958688 2316 92-93 -1.8487299067916183 2316 94-95 -1.856547647646245 2316 96-97 -1.8651970037198886 2316 98-99 -2.0989933152582303 2316 100-101 -2.110647203900868 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4206.0 3 655.0 4 677.0 5 790.0 6 822.0 7 1113.0 8 1317.0 9 1734.0 10 2304.0 11 3084.0 12 4535.0 13 5782.0 14 7030.0 15 9092.0 16 12191.0 17 16302.0 18 13741.0 19 9066.0 20 8460.0 21 8902.0 22 9626.0 23 10592.0 24 11635.0 25 13261.0 26 15056.0 27 17677.0 28 21107.0 29 24602.0 30 30483.0 31 37606.0 32 47496.0 33 62051.0 34 84815.0 35 121713.0 36 168253.0 37 210781.0 38 139180.0 39 12881.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.896550701943745 19.332791610503612 15.297683088657127 21.472974598895515 2 15.196820540978678 20.928300933007367 40.410941592257046 23.463936933756912 3 18.556095435232596 26.029676435341543 30.590434687874495 24.823793441551366 4 12.330358644769193 17.005317484597075 37.34624311563982 33.318080754993915 5 12.816860771719035 38.178012972104135 33.99542027717173 15.0097059790051 6 30.30385354844103 37.08317660007245 17.350720276407813 15.262249575078716 7 27.132497766539366 34.521551806708565 20.770226097617467 17.5757243291346 8 26.276293665313215 34.00149596446605 20.155465364242968 19.56674500597776 9 26.862171773286292 16.52914726360188 20.801710836575403 35.80697012653643 10-11 24.337121830600488 27.359995193080128 26.819521493767766 21.483361482551622 12-13 25.844140690985906 25.077365413785234 27.901442470956404 21.177051424272452 14-15 23.01955433496366 25.942952228281648 27.06603996200066 23.971453474754036 16-17 20.814849226726135 29.439701357501523 27.782303411757177 21.96314600401516 18-19 20.734823349222637 27.222953390455885 31.450460244160368 20.591763016161117 20-21 21.58860525567693 26.107862270842848 31.874409843195146 20.429122630285075 22-23 21.585133480273445 26.071392345328704 31.666710925735746 20.676763248662105 24-25 21.251644540647472 26.379650969919815 31.57944687709241 20.789257612340304 26-27 20.542619291921135 26.747962772466533 31.740263991777518 20.969153943834815 28-29 20.601446407556743 26.762675875748794 31.97086635119291 20.665011365501556 30-31 20.68084824767871 26.760746122183125 31.499942092893445 21.058463537244716 32-33 20.40386870304922 26.926133164858772 31.37314749463031 21.296850637461702 34-35 21.107510350553852 26.822095869052486 31.31790884197886 20.752484938414803 36-37 21.229205191693566 26.466301701892036 31.080598709389832 21.223894397024562 38-39 20.71444956574389 26.982119173300685 31.07243608689388 21.230995174061547 40-41 20.932416173175067 26.756458631155006 31.308973044127814 21.002152151542113 42-43 20.718931485141617 26.92231674082072 30.804955986452008 21.553795787585656 44-45 20.759636511974268 27.30296081746059 30.573447507082165 21.363955163482977 46-47 21.216839029266378 27.45606908784093 29.943332366814086 21.383759516078612 48-49 21.03641095611303 27.65567845304156 29.601348921568032 21.70656166927738 50-51 21.50834060764641 27.913727009724 29.21374290074209 21.364189481887504 52-53 21.442979587321965 27.877889734784116 29.197045008574595 21.48208566931932 54-55 21.26128353717995 28.172493592065308 29.153360334884237 21.412862535870502 56-57 21.250902120080127 28.318604871840186 28.921599779921873 21.508893228157813 58-59 21.109523289254952 27.711747507015698 29.205862240227532 21.972866963501815 60-61 21.044659377738082 28.079742080047637 29.326816562834473 21.54878197937981 62-63 21.161127270163 28.1821023095788 29.496600795193668 21.160169625064533 64-65 21.08266386692157 28.046725099157637 29.326154633682606 21.54445640023819 66-67 20.851456868923364 28.100435835393245 29.24484651743015 21.803260778253236 68-69 21.141656411298943 28.224324614928538 29.21053765697025 21.423481316802278 70-71 21.310973751795586 28.036956427110088 28.871588386366604 21.780481434727726 72-73 21.07919459114301 28.29329634106477 28.491562480820853 22.135946586971368 74-75 20.942751592168158 28.807024126609086 28.535100827522626 21.715123453700127 76-77 21.15083255124765 28.10907352882283 28.909195064067184 21.830898855862337 78-79 21.048901611712427 28.115988967361776 28.878313622243585 21.95679579868221 80-81 21.208653448538534 28.403551918514815 28.590506453087254 21.797288179859404 82-83 21.20497209648726 28.199327843313753 28.618109055485352 21.977591004713627 84-85 21.131534276387377 28.156735582154518 28.461240478781285 22.250489662676824 86-87 21.2241773125951 28.58470955678815 28.227857687526768 21.96325544308998 88-89 21.315734494015235 28.129488574537543 28.235647442872686 22.31912948857454 90-91 21.04780455955166 27.932139942325477 28.462571413025735 22.55748408509712 92-93 21.067924950413946 27.849582223622765 28.58750801407256 22.494984811890728 94-95 20.977001988320794 27.47547679654011 28.7515190979779 22.796002117161194 96-97 20.912430188813932 27.344482810702488 28.7972693167677 22.94581768371588 98-99 20.821024123367337 27.430542104353663 28.68287136888281 23.06556240339619 100-101 21.07290931823849 26.885400859159546 29.24937342976647 22.79231639283549 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2491.0 1 1571.0 2 778.5 3 905.0 4 785.0 5 888.0 6 1685.0 7 3235.5 8 4814.0 9 5256.5 10 4370.0 11 3544.0 12 3536.5 13 3547.5 14 3506.0 15 3453.0 16 3383.5 17 3384.5 18 3464.5 19 3606.5 20 3867.5 21 4245.0 22 4713.0 23 5460.0 24 6276.5 25 7063.5 26 8392.0 27 10151.5 28 11180.5 29 12495.0 30 14683.5 31 17039.5 32 20471.5 33 22661.0 34 23235.0 35 25154.5 36 29025.0 37 32227.5 38 33009.5 39 33187.5 40 32991.5 41 32514.5 42 31928.5 43 33888.5 44 36894.0 45 38101.5 46 37972.0 47 38841.5 48 42135.5 49 41503.0 50 38582.5 51 36764.0 52 34522.5 53 34064.5 54 34898.5 55 33436.5 56 30407.5 57 27780.5 58 25834.0 59 23565.5 60 19706.0 61 16108.5 62 13128.0 63 9483.5 64 6546.0 65 4918.5 66 3470.0 67 2506.0 68 1920.0 69 1383.5 70 967.0 71 653.0 72 453.0 73 347.0 74 248.0 75 174.0 76 140.5 77 97.0 78 70.0 79 45.5 80 27.5 81 23.0 82 18.0 83 12.5 84 10.5 85 8.5 86 6.0 87 3.0 88 2.5 89 2.5 90 1.5 91 3.0 92 3.0 93 1.5 94 1.0 95 1.0 96 2.0 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31817656409289263 2 0.2828042992510988 3 0.28193520183483844 4 0.28436867460036724 5 0.2944502046289867 6 0.1886810490701092 7 0.1892025075198654 8 0.19085379261075996 9 0.18902868803661335 10-11 0.19767620732840324 12-13 0.18885486855336128 14-15 0.18928941726149143 16-17 0.19315690076384973 18-19 0.18707321885002767 20-21 0.18159790512758783 22-23 0.18064189796970154 24-25 0.1844224717304338 26-27 0.179077522620433 28-29 0.1901150598069387 30-31 0.19363490434279287 32-33 0.19776311707002925 34-35 0.19559037352937853 36-37 0.17512312937644867 38-39 0.1677792562090492 40-41 0.17390639299368427 42-43 0.1832926450892954 44-45 0.1855957532423852 46-47 0.18842031984523114 48-49 0.1865083055294585 50-51 0.18872450394092222 52-53 0.21379796440003165 54-55 0.1775566021419775 56-57 0.16825725978799239 58-59 0.16908290233343964 60-61 0.16430286654400805 62-63 0.17103837152002532 64-65 0.1688656279793746 66-67 0.1656065126683985 68-69 0.1767309595965302 70-71 0.17112528126165133 72-73 0.16604106137652863 74-75 0.16943054129994378 76-77 0.1721681981611637 78-79 0.17616604627596102 80-81 0.1689525377210006 82-83 0.16760543672579717 84-85 0.1624343070990484 86-87 0.15843645898425107 88-89 0.1624343070990484 90-91 0.16786616595067524 92-93 0.16152175481197512 94-95 0.16608451624734163 96-97 0.1647374152521382 98-99 0.1668232490511629 100-101 0.14822456434319264 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1150619.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.246805611119555 #Duplication Level Percentage of deduplicated Percentage of total 1 68.8474719103638 29.085857626141525 2 14.405238475258948 12.171506192921697 3 6.053487856981055 7.672215742894539 4 3.204810551363006 5.415720335355912 5 1.917222026405368 4.049825313145216 6 1.2365049700792747 3.134303106487339 7 0.8616793408549382 2.5482239728551344 8 0.5865389006167399 1.9823515934172142 9 0.44074373913323167 1.675801356433166 >10 2.1965145144839897 16.604807803277726 >50 0.16053342640179902 4.665103832987851 >100 0.08242043372698785 6.59174072515009 >500 0.004970075876976228 1.4239453933672814 >1k 0.001242518969244057 0.8619261498804656 >5k 4.141729897480191E-4 1.1982278267116175 >10k+ 2.0708649487400954E-4 0.9184430289733179 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10498 0.9123784675900536 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7954 0.6912800848934356 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5742 0.49903573641665916 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2489 0.21631834690718646 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1805 0.15687208363498256 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1586 0.1378388502188822 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1501 0.13045152218066972 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1335 0.11602450507074888 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.345487081301456E-5 0.0 12-13 0.0 0.0 0.0 8.690974162602912E-5 0.0 14-15 0.0 0.0 0.0 8.690974162602912E-5 8.690974162602912E-5 16-17 0.0 0.0 0.0 3.0418409569110195E-4 1.7381948325205824E-4 18-19 0.0 0.0 0.0 3.476389665041165E-4 1.7381948325205824E-4 20-21 0.0 0.0 0.0 3.91093837317131E-4 1.7381948325205824E-4 22-23 0.0 0.0 0.0 9.125522870733057E-4 1.7381948325205824E-4 24-25 0.0 0.0 0.0 0.0015209204784555094 1.7381948325205824E-4 26-27 0.0 0.0 0.0 0.002563837377967859 1.7381948325205824E-4 28-29 0.0 0.0 0.0 0.004649671176992558 1.7381948325205824E-4 30-31 0.0 0.0 0.0 0.009907710545367321 1.7381948325205824E-4 32-33 0.0 0.0 0.0 0.015861027846750315 3.476389665041165E-4 34-35 0.0 0.0 0.0 0.023074536401710734 4.345487081301456E-4 36-37 0.0 0.0 0.0 0.031200597243744455 4.345487081301456E-4 38-39 0.0 0.0 0.0 0.038587925281956925 4.345487081301456E-4 40-41 0.0 0.0 0.0 0.04945164298521057 4.345487081301456E-4 42-43 0.0 0.0 0.0 0.061966645779358766 4.345487081301456E-4 44-45 0.0 0.0 0.0 0.07456855831513298 5.649133205691893E-4 46-47 0.0 0.0 0.0 0.08921284977911889 7.82187674634262E-4 48-49 0.0 0.0 0.0 0.10637752375025963 8.690974162602912E-4 50-51 0.0 0.0 0.0 0.1298431539892875 8.690974162602912E-4 52-53 0.0 0.0 0.0 0.17707859856303432 9.560071578863204E-4 54-55 0.0 0.0 0.0 0.23361338549076627 0.0010429168995123494 56-57 0.0 0.0 0.0 0.2884968873276037 0.0010429168995123494 58-59 0.0 0.0 0.0 0.3816641303507069 0.0010429168995123494 60-61 0.0 0.0 0.0 0.48426108034023424 0.0011732815119513931 62-63 0.0 0.0 0.0 0.5646091364734982 0.0012167363827644076 64-65 0.0 0.0 0.0 0.6377002291809887 0.0012167363827644076 66-67 0.0 0.0 0.0 0.7938770348829629 0.0012167363827644076 68-69 0.0 0.0 0.0 0.9584840855226622 0.0012167363827644076 70-71 0.0 0.0 0.0 1.0492613106510496 0.001390555866016466 72-73 0.0 0.0 0.0 1.220560411395953 0.0019989240573986696 74-75 0.0 0.0 0.0 1.4490026672599705 0.0019989240573986696 76-77 0.0 0.0 0.0 1.67718419389911 0.0019989240573986696 78-79 0.0 0.0 0.0 1.9257026000787403 0.0019989240573986696 80-81 0.0 0.0 0.0 2.202205943061952 0.0019989240573986696 82-83 0.0 0.0 0.0 2.428910004093449 0.002085833799024699 84-85 0.0 0.0 0.0 2.688335582847146 0.0021292886698377135 86-87 0.0 0.0 0.0 3.0729111895423245 0.002172743540650728 88-89 0.0 0.0 0.0 3.569426543451829 0.002172743540650728 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5720 0.0 38.083897 1 GTATCAA 8935 0.0 34.8141 1 TATCAAC 11260 0.0 27.313217 2 GGTATAG 105 6.8435096E-5 27.179024 1 TCAACGC 11320 0.0 27.12882 4 ATCAACG 11330 0.0 27.102514 3 CAACGCA 11485 0.0 26.73907 5 AACGCAG 11775 0.0 26.102705 6 TATAGGG 295 0.0 25.781307 2 GAGTACT 6860 0.0 24.512268 12-13 GTACATG 6715 0.0 23.728523 1 ACGCAGA 13065 0.0 23.48904 7 CGCAGAG 13165 0.0 23.309605 8 GTACTTT 7265 0.0 22.98133 14-15 TACATGG 6835 0.0 22.810892 2 ACATGGG 6625 0.0 22.601206 3 AGTACTT 7245 0.0 22.455696 12-13 GCAGAGT 13320 0.0 22.32607 9 CATGGGA 3830 0.0 22.093576 4 GTGTAAG 335 1.8189894E-12 21.296997 1 >>END_MODULE