FastQCFastQC Report
Thu 26 May 2016
SRR937815_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937815_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150619
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136281.1844059588795248No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110550.9607871936757519No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79810.6936266479173384No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29460.2560360988302818No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24230.21058230395986857No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24110.20953938706035619No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22250.1933741751179148No Hit
TCCCAGGACCGAGTGGGAGTAGGGGATTACTGAGTGGCATCCCCAAGCTG14370.12488929871660386No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12540.1089848159990405No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11580.10064148080294172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64350.038.9236561
GTATCAA103800.036.1727331
TATCAAC134300.027.882152
ATCAACG136000.027.498683
TCAACGC136750.027.3131184
CAACGCA138500.026.9336935
TAAGGTG4500.026.3999775
AACGCAG142000.026.2895856
GTAAGGT4000.026.1359774
GGTAAGG3950.025.2637733
GTGTACG2101.2569217E-924.8957351
TGTACGC1351.3383664E-524.6399772
ACGCAGA154800.023.9347977
TATCACG1000.001649608323.759982
CTATACG1201.7059522E-423.759984
CGCAGAG156300.023.6139248
ATATAGG3650.023.43452
AGTACTT100100.023.323612-13
GGACCGA6750.023.2198646
GAGTACT96650.023.12404812-13
Produced by FastQC (version 0.11.5)