##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937815_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1150619 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.095725865816576 31.0 31.0 34.0 31.0 34.0 2 32.36442297580694 33.0 31.0 34.0 31.0 34.0 3 32.43510840686622 34.0 31.0 34.0 31.0 34.0 4 36.1084051280224 37.0 35.0 37.0 35.0 37.0 5 36.025345487950396 37.0 35.0 37.0 35.0 37.0 6 36.08081563054321 37.0 35.0 37.0 35.0 37.0 7 36.015037123496136 37.0 35.0 37.0 35.0 37.0 8 35.99853730904844 37.0 35.0 37.0 35.0 37.0 9 37.66786833869421 39.0 37.0 39.0 35.0 39.0 10-11 37.585200661556954 39.0 37.0 39.0 35.0 39.0 12-13 37.47988560939807 39.0 37.0 39.0 35.0 39.0 14-15 38.61567643155554 40.0 38.0 41.0 34.0 41.0 16-17 38.5905051976371 40.0 38.0 41.0 34.0 41.0 18-19 38.639922511274364 40.0 38.0 41.0 34.0 41.0 20-21 38.56576764332938 40.0 38.0 41.0 34.0 41.0 22-23 38.49513783450473 40.0 38.0 41.0 34.0 41.0 24-25 38.48938875509617 40.0 38.0 41.0 34.0 41.0 26-27 38.3168086047597 40.0 38.0 41.0 34.0 41.0 28-29 38.12835221737169 40.0 37.5 41.0 33.5 41.0 30-31 38.0604935256588 40.0 37.0 41.0 34.0 41.0 32-33 37.88149291815971 40.0 36.5 41.0 33.0 41.0 34-35 37.68828908613538 40.0 36.0 41.0 33.0 41.0 36-37 37.49316150698016 40.0 36.0 41.0 33.0 41.0 38-39 37.306621479395005 40.0 36.0 41.0 33.0 41.0 40-41 37.06360489440901 40.0 35.0 41.0 32.0 41.0 42-43 36.76621540231823 39.0 35.0 41.0 31.0 41.0 44-45 36.34224665158493 39.0 35.0 41.0 30.5 41.0 46-47 35.79884001567852 39.0 35.0 41.0 29.0 41.0 48-49 35.31689073446553 39.0 35.0 40.5 26.5 41.0 50-51 34.973677646553725 39.0 35.0 40.0 25.5 41.0 52-53 34.68958491038302 38.0 34.5 40.0 24.0 41.0 54-55 34.429090341807324 38.0 34.0 40.0 23.5 41.0 56-57 34.11705221276547 38.0 34.0 40.0 23.0 41.0 58-59 33.8185029101727 37.5 34.0 40.0 22.0 41.0 60-61 33.463473139240705 37.0 33.0 40.0 21.0 41.0 62-63 33.049682388349225 36.5 33.0 39.5 19.5 41.0 64-65 32.593935525139074 36.0 32.0 39.0 17.5 40.5 66-67 32.1449059158592 35.0 32.0 39.0 13.5 40.0 68-69 31.671399046947773 35.0 31.0 38.0 9.0 40.0 70-71 31.265665263653737 35.0 31.0 37.5 7.5 40.0 72-73 30.788212692472488 35.0 30.5 37.0 4.5 39.0 74-75 30.317302252092134 34.0 30.0 36.0 2.0 39.0 76-77 29.120378248577502 33.5 28.5 35.0 2.0 37.0 78-79 29.689313751989147 34.0 30.0 35.0 2.0 37.5 80-81 29.52056067212518 34.0 30.0 35.0 2.0 37.0 82-83 29.154097490133573 34.0 29.0 35.0 2.0 36.5 84-85 28.77857353302874 34.0 29.0 35.0 2.0 36.0 86-87 28.42502731138631 34.0 29.0 35.0 2.0 36.0 88-89 27.97057192693672 34.0 28.0 35.0 2.0 35.0 90-91 27.437204669834237 33.0 26.5 35.0 2.0 35.0 92-93 26.9954407149543 33.0 25.5 35.0 2.0 35.0 94-95 26.523245748592714 33.0 25.0 35.0 2.0 35.0 96-97 25.94610075098708 32.5 24.5 34.5 2.0 35.0 98-99 25.07498051049044 32.0 19.0 34.0 2.0 35.0 100-101 22.43521052581263 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3313919104151566 1101 2 0.33306192488951325 1101 3 0.32805196782973667 1101 4 0.17584615156028605 1101 5 0.19497676357352134 1101 6 0.1606994061784448 1101 7 0.2249158914105891 1101 8 0.2206805421127882 1101 9 0.3117307725323215 1101 10-11 0.25784271442487494 1101 12-13 0.3178725980400188 1101 14-15 0.36890696248202204 1101 16-17 0.39415154449053347 1101 18-19 0.41169137238240694 1101 20-21 0.3422099734455344 1101 22-23 0.3460519886431186 1101 24-25 0.38100175560978045 1101 26-27 0.28263890455575336 1101 28-29 0.3027780921878147 1101 30-31 0.2004772389224314 1101 32-33 0.3871492678535091 1101 34-35 0.3008383105611472 1101 36-37 0.32981604683115506 1101 38-39 0.415276054170846 1101 40-41 0.4291190314996385 1101 42-43 0.22240203364794553 1101 44-45 0.1310638887476827 1101 46-47 0.08627135290840471 1101 48-49 0.05502063179044825 1101 50-51 0.2646257725610681 1101 52-53 0.3674779916091282 1101 54-55 0.1682667033153109 1101 56-57 0.19657959047045637 1101 58-59 -0.14166097038775405 1101 60-61 0.1574032457478367 1101 62-63 0.02895814534797836 1101 64-65 0.008347438985566669 1101 66-67 -0.12379881157971795 1101 68-69 -0.00982580354888185 1101 70-71 0.07983186464373304 1101 72-73 -0.04369803746419976 1101 74-75 -0.25038740031675744 1101 76-77 -0.14867366865066955 1101 78-79 -0.055554391655952884 1101 80-81 -0.13686159646123386 1101 82-83 -0.2814841386245668 1101 84-85 -0.31678811654992245 1101 86-87 -0.3212157754741618 1101 88-89 -0.26202004088684916 1101 90-91 -0.2703486624402629 1101 92-93 -0.40889601843349865 1101 94-95 -0.357346672874602 1101 96-97 -0.24141034018536445 1101 98-99 -0.30453637122750266 1101 100-101 -0.05829646238410646 1102 1 0.26824065431887334 1102 2 0.20705827992915005 1102 3 0.039367569187206186 1102 4 0.04641651222593879 1102 5 0.035269433885147805 1102 6 0.07712880885873119 1102 7 0.1499136346580201 1102 8 0.13895743223455526 1102 9 0.10783378779773045 1102 10-11 0.016956030240926623 1102 12-13 0.3140431329025759 1102 14-15 0.3229652745286842 1102 16-17 0.1891707085893799 1102 18-19 0.07724430115929692 1102 20-21 0.11995430655685624 1102 22-23 0.11954173322752126 1102 24-25 -0.01643402453128573 1102 26-27 -0.06348869101466903 1102 28-29 -0.08697070075452729 1102 30-31 0.1817126462723735 1102 32-33 0.29355502952579826 1102 34-35 0.2151234933337136 1102 36-37 0.12810780712255365 1102 38-39 0.15870829426005173 1102 40-41 0.0827291646846362 1102 42-43 0.19638228645302291 1102 44-45 0.18588559309601038 1102 46-47 0.09328771863406615 1102 48-49 -0.006640252710177208 1102 50-51 0.1418550781400043 1102 52-53 0.3567343245447816 1102 54-55 0.22129306950451877 1102 56-57 0.23489467460463231 1102 58-59 0.3742640785898743 1102 60-61 0.32385369112100193 1102 62-63 0.3111671889484384 1102 64-65 0.3540770531906716 1102 66-67 0.40672193712539695 1102 68-69 0.5221804372868704 1102 70-71 0.1705883890983948 1102 72-73 0.014543561926842585 1102 74-75 -0.09897546612122454 1102 76-77 0.007143507554133066 1102 78-79 0.4305995164295666 1102 80-81 0.40409266831317936 1102 82-83 0.3941985595244084 1102 84-85 0.41984977347181385 1102 86-87 -0.04746070997954632 1102 88-89 0.12532561553538457 1102 90-91 0.3290692173833918 1102 92-93 0.16841605507076096 1102 94-95 -0.04752107715291842 1102 96-97 -0.050863876699619226 1102 98-99 -0.0447463346071153 1102 100-101 -0.10875501010893274 1103 1 0.3687093339042704 1103 2 0.24804842993236775 1103 3 0.26155323840757916 1103 4 0.15357868308797862 1103 5 0.16063864781677495 1103 6 0.1226654083565748 1103 7 0.1310711702274503 1103 8 0.0913909608510508 1103 9 0.15542665459744143 1103 10-11 0.1939826831733953 1103 12-13 0.3267969473621193 1103 14-15 0.2770285591485262 1103 16-17 0.2785346434377729 1103 18-19 0.2993366207369732 1103 20-21 0.1627725000803224 1103 22-23 0.21750928617930043 1103 24-25 0.17853636109929738 1103 26-27 0.30925096005080377 1103 28-29 0.23165319675400298 1103 30-31 0.2999724801743824 1103 32-33 0.3693510766512773 1103 34-35 0.361355064826661 1103 36-37 0.38168245464717643 1103 38-39 0.4320663062345531 1103 40-41 0.3735232804381994 1103 42-43 0.4968174889251813 1103 44-45 0.5725478605266474 1103 46-47 0.48550332411915775 1103 48-49 0.31960355080492064 1103 50-51 0.385841107883131 1103 52-53 0.5658661728677075 1103 54-55 0.45917757346383326 1103 56-57 0.6858915844095677 1103 58-59 0.5416409536301146 1103 60-61 0.6860570645839488 1103 62-63 0.5491930049530751 1103 64-65 0.5349822228295054 1103 66-67 0.44451534470925225 1103 68-69 0.41049113064939036 1103 70-71 0.39339563818099066 1103 72-73 0.3953492659119071 1103 74-75 0.48224419524489903 1103 76-77 0.5160342562826479 1103 78-79 0.606719390246603 1103 80-81 0.6863462574459902 1103 82-83 0.5801343092186109 1103 84-85 0.6086932035883379 1103 86-87 0.5159681189652829 1103 88-89 0.6824205102756125 1103 90-91 0.7447274933120624 1103 92-93 0.7890192868997694 1103 94-95 0.6817684998766325 1103 96-97 0.7067260930422243 1103 98-99 0.8768721550600453 1103 100-101 0.8997365884232238 1104 1 0.31693444212938005 1104 2 0.20587155918406808 1104 3 0.2281333249876667 1104 4 0.11696767504840011 1104 5 0.13550568554095577 1104 6 0.1462399290739569 1104 7 -0.009238044367478437 1104 8 0.05502732448741199 1104 9 0.1732126780977552 1104 10-11 0.16106988168915848 1104 12-13 0.20353164123966394 1104 14-15 0.22547630188198298 1104 16-17 0.3081821387995518 1104 18-19 0.2864485564203392 1104 20-21 0.218134281156388 1104 22-23 0.2670330957031126 1104 24-25 0.17340339882348843 1104 26-27 0.11589288954987609 1104 28-29 0.11282821221473682 1104 30-31 0.12061564653183154 1104 32-33 0.06888107046697911 1104 34-35 0.15080466284768335 1104 36-37 0.08279562718892919 1104 38-39 0.13133655979052605 1104 40-41 0.2224286607721453 1104 42-43 0.0996993689499206 1104 44-45 0.30643778013084955 1104 46-47 0.15369132659288454 1104 48-49 0.10774207894345267 1104 50-51 0.21216145420347488 1104 52-53 0.31430154701737223 1104 54-55 0.19366118509029917 1104 56-57 0.37349826344481585 1104 58-59 0.23529587014217412 1104 60-61 0.2906210720051021 1104 62-63 0.06977432839153863 1104 64-65 -0.018140844579285442 1104 66-67 -0.03605361014541586 1104 68-69 0.2572938517378276 1104 70-71 0.12211548975798436 1104 72-73 -0.0713916122575462 1104 74-75 0.016233063519482727 1104 76-77 -0.07734855138587449 1104 78-79 -0.24713342360621482 1104 80-81 -0.14549047724788622 1104 82-83 0.18572490909492245 1104 84-85 0.12587316648258806 1104 86-87 0.058169726881175166 1104 88-89 0.20003337360276063 1104 90-91 0.2986050443324686 1104 92-93 0.47373171732648345 1104 94-95 0.4405069043293217 1104 96-97 0.3665405642852626 1104 98-99 0.39925929173289276 1104 100-101 0.3328225500806141 1105 1 0.22372594519995204 1105 2 0.1611760996048872 1105 3 0.12454327163016643 1105 4 0.012145365574923517 1105 5 0.0023092695538409203 1105 6 -0.037809200091452055 1105 7 0.0392629120404564 1105 8 -0.014788441142307818 1105 9 0.09803700106161273 1105 10-11 0.04910370832764244 1105 12-13 0.09721934888086281 1105 14-15 0.02883681733552379 1105 16-17 0.09868651814114315 1105 18-19 0.18089333644651617 1105 20-21 -0.03847097405351718 1105 22-23 0.012326561926812474 1105 24-25 -0.09082291749585636 1105 26-27 -0.05339735508920285 1105 28-29 -0.04544219954404838 1105 30-31 -0.19708494744782712 1105 32-33 -0.21319282463016975 1105 34-35 -0.024729415477089844 1105 36-37 -0.022797849102232703 1105 38-39 -0.09858390733924693 1105 40-41 -0.1252427375969276 1105 42-43 -0.13601592350955372 1105 44-45 -0.21739993254407608 1105 46-47 -0.46076150739484234 1105 48-49 -0.5482369807565348 1105 50-51 -0.5647236761700256 1105 52-53 -0.36248693581762126 1105 54-55 -0.40123058980147874 1105 56-57 -0.3690870582102761 1105 58-59 -0.3284492219750135 1105 60-61 -0.39240520404442947 1105 62-63 -0.4079403600208238 1105 64-65 -0.39589580279935177 1105 66-67 -0.2420437440425296 1105 68-69 -0.18341489687789903 1105 70-71 -0.25124603244074706 1105 72-73 -0.20003753997324125 1105 74-75 -0.2574425631793993 1105 76-77 -0.4884650318046795 1105 78-79 -0.3397922376603759 1105 80-81 -0.3108060918658282 1105 82-83 -0.2958315189731344 1105 84-85 -0.1641781775222455 1105 86-87 -0.11165409271008286 1105 88-89 0.08994780026137406 1105 90-91 0.05275786825273343 1105 92-93 0.03867282273270689 1105 94-95 -0.19542181811102566 1105 96-97 -0.16918278213575633 1105 98-99 -0.061552144892495164 1105 100-101 0.04561224100983807 1106 1 0.15745679497401 1106 2 0.14396679727930461 1106 3 0.06893983285578287 1106 4 0.14814779035173586 1106 5 0.07485859171850962 1106 6 -0.010694684495732076 1106 7 0.029314714090496352 1106 8 -0.08792522059783181 1106 9 0.019404795616885906 1106 10-11 -0.11637518509240152 1106 12-13 -0.12666470270075791 1106 14-15 0.1395907024892793 1106 16-17 0.07428665040169591 1106 18-19 0.1735672039655256 1106 20-21 0.08608374875041136 1106 22-23 0.16334882578661336 1106 24-25 0.09491608834564857 1106 26-27 0.11661917747995432 1106 28-29 0.17830132519969766 1106 30-31 0.191553871024567 1106 32-33 0.024651879436838442 1106 34-35 -0.14860189426029535 1106 36-37 0.278751803713817 1106 38-39 0.1799568178894475 1106 40-41 0.0162121284892649 1106 42-43 -0.30053041706185724 1106 44-45 -0.13991949608424648 1106 46-47 -0.34054658399573867 1106 48-49 -0.28820281344693655 1106 50-51 -0.2108853646964164 1106 52-53 -0.38160795422388816 1106 54-55 -0.17151494999104955 1106 56-57 -0.12659560003767467 1106 58-59 0.07436462129622612 1106 60-61 -0.07958419665988714 1106 62-63 -0.35243336123321 1106 64-65 -0.5042623849662249 1106 66-67 -0.6289912806897249 1106 68-69 -0.30735295485210656 1106 70-71 -0.3453769703885037 1106 72-73 -0.3421195415162792 1106 74-75 -0.06694383067636878 1106 76-77 -0.17243174623210322 1106 78-79 -0.36373084672022316 1106 80-81 -0.6588960290420651 1106 82-83 -0.7590300200201945 1106 84-85 -0.5767396235709583 1106 86-87 -0.2274471049441864 1106 88-89 -0.24053770618632697 1106 90-91 -0.5481789773330696 1106 92-93 -0.38869519225682225 1106 94-95 -0.3326250584945818 1106 96-97 -0.2871354989879542 1106 98-99 -0.2870822892769347 1106 100-101 -0.7187782582737476 1107 1 0.26995104577889606 1107 2 0.17350266225820832 1107 3 0.0743440762617027 1107 4 0.03553855564459241 1107 5 0.16098653380661432 1107 6 0.18627773936493242 1107 7 0.1451484973608359 1107 8 0.18078216036882822 1107 9 0.2532170618996048 1107 10-11 0.2610794712557123 1107 12-13 0.32587944111910616 1107 14-15 0.3204828775847588 1107 16-17 0.20930274814801209 1107 18-19 0.24092277418569097 1107 20-21 0.37330853728001046 1107 22-23 0.20558041336435195 1107 24-25 0.2911386171916135 1107 26-27 0.3728508050520958 1107 28-29 0.32600564659470166 1107 30-31 0.13048742032766114 1107 32-33 0.14479755904169878 1107 34-35 0.11046683611745323 1107 36-37 0.2425043783533738 1107 38-39 0.3128232165936282 1107 40-41 0.20911012277006336 1107 42-43 0.32440209238681916 1107 44-45 0.397827719305603 1107 46-47 0.1882493845686568 1107 48-49 0.04419065146797152 1107 50-51 0.08214611089699275 1107 52-53 -0.1623711473768381 1107 54-55 -0.1285934589997879 1107 56-57 -0.14112856542251961 1107 58-59 -0.0541473870226028 1107 60-61 -0.07422494695484261 1107 62-63 -0.19561281610952364 1107 64-65 -0.05229066100758217 1107 66-67 0.041669826755239114 1107 68-69 0.1189273659029837 1107 70-71 -0.06616605991596813 1107 72-73 -0.10378790794498016 1107 74-75 0.032699719226645385 1107 76-77 5.365382080775305E-4 1107 78-79 -0.0747363059559305 1107 80-81 -0.07427287628344814 1107 82-83 -0.004515652031713557 1107 84-85 0.13857523234921132 1107 86-87 -0.10816130495618381 1107 88-89 0.08442429913293026 1107 90-91 0.3191595952667683 1107 92-93 0.2702575543586505 1107 94-95 0.24608392224811126 1107 96-97 0.028587799750223297 1107 98-99 -0.3148633999214425 1107 100-101 -0.5616127614433033 1108 1 0.12936337865470193 1108 2 0.07770173703833905 1108 3 -0.04071870651497278 1108 4 0.04630059112830054 1108 5 0.05966987476539032 1108 6 0.13024347572810768 1108 7 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70-71 -0.07061049439252187 2316 72-73 -0.13585511945669992 2316 74-75 -0.10471367223944483 2316 76-77 0.05336774389455812 2316 78-79 0.0651602123585775 2316 80-81 -0.2571944359397982 2316 82-83 -0.10821264402051511 2316 84-85 0.03343987334057985 2316 86-87 0.08644324877624499 2316 88-89 0.09180826537765441 2316 90-91 0.20867895341417508 2316 92-93 0.20413752696687837 2316 94-95 0.11137494486426291 2316 96-97 -0.07145496251252936 2316 98-99 0.2127896545899759 2316 100-101 0.37924598254231867 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 10.0 5 31.0 6 75.0 7 164.0 8 385.0 9 780.0 10 1254.0 11 2220.0 12 3450.0 13 5030.0 14 6246.0 15 9240.0 16 13150.0 17 18825.0 18 16711.0 19 9525.0 20 7620.0 21 7801.0 22 7918.0 23 8184.0 24 9306.0 25 10620.0 26 12576.0 27 15026.0 28 18752.0 29 22580.0 30 28010.0 31 35162.0 32 44467.0 33 57720.0 34 80549.0 35 117909.0 36 173293.0 37 238775.0 38 156528.0 39 10720.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.46837410402132 19.93970887103644 15.944552327936453 20.647364697005784 2 15.794628804148028 21.69962428918695 39.36429000390225 23.141456902762776 3 18.97430861127793 26.111336593607444 30.927005377105715 23.987349418008915 4 12.923044031082402 17.742884482178724 36.58135316729517 32.75271831944371 5 12.764344346032757 38.51169883763695 33.639407489586205 15.084549326744087 6 30.426665994564665 37.47252565792847 16.678935425192876 15.42187292231399 7 27.042748294613595 34.989514339672816 20.3759020144809 17.591835351232685 8 26.826082308739906 34.06635906412114 19.639341954200304 19.468216672938652 9 27.082987504986445 16.50598503935708 20.49305634619279 35.917971109463686 10-11 24.629699318366896 27.565858029460667 26.255519854965026 21.548922797207414 12-13 26.25471719431451 25.328095965679857 27.57340223783177 20.843784602173866 14-15 23.22310860502043 26.013780408632226 27.18436771859321 23.57874326775414 16-17 20.6904283694255 29.299316281062627 28.067674877609356 21.942580471902513 18-19 20.565973619417026 26.703148479209887 32.48129919634562 20.249578705027467 20-21 21.30222949560193 25.570106177631345 33.165800321392226 19.9618640053745 22-23 21.45934086181355 25.312562874339967 33.00105987397103 20.227036389875444 24-25 21.208447293930128 25.640373816830447 32.90117389192059 20.25000499731883 26-27 20.388117892863228 26.215709314412255 33.006360137805885 20.389812654918636 28-29 20.44935451405607 26.02532926127623 33.22492669080467 20.300389533863026 30-31 20.504702251570674 26.08895733513874 32.82272411632348 20.58361629696711 32-33 20.31762903272065 26.315922125395115 32.71252256394167 20.65392627794257 34-35 21.09925179403434 26.06231949933036 32.538181622239854 20.300247084395444 36-37 21.294929077305348 25.740623090701614 32.327903502375676 20.63654432961736 38-39 20.636891968583868 26.30710078662007 32.412205951752924 20.643801293043136 40-41 20.949740941423602 26.072334768950515 32.47299146495094 20.504932824674942 42-43 20.747557541200194 26.260039287949375 31.956053125651902 21.036350045198525 44-45 20.826094476103734 26.658998330463863 31.6331470278172 20.881760165615205 46-47 21.39413654737146 27.06534482743636 30.750361327250808 20.79015729794137 48-49 21.18038203784224 27.198577461349068 30.361874782182458 21.259165718626235 50-51 21.687630744842558 27.575852649747656 29.970302941286388 20.7662136641234 52-53 21.625751008804826 27.624652469670675 29.80691262702945 20.94268389449505 54-55 21.57660697547793 27.942324831948785 29.63685120107351 20.844216991499774 56-57 21.521198589628714 28.075844393322203 29.362238933999873 21.04071808304921 58-59 21.24495597587038 27.456134480657802 29.640089377978292 21.65882016549353 60-61 21.313049758434374 27.895332859964938 29.75129039238879 21.040326989211895 62-63 21.32626003916153 27.93613698365836 30.064686920692253 20.672916056487857 64-65 21.399438041610647 27.769965557669394 29.840590151909534 20.990006248810424 66-67 21.2456947086742 27.943393947084132 29.650953095681544 21.159958248560123 68-69 21.506945392002045 28.06467649152326 29.606629127452265 20.82174898902243 70-71 21.5612205256475 27.915539374892994 29.158218315532768 21.36502178392674 72-73 21.481350473093176 28.04668617500667 28.750524717565067 21.721438634335083 74-75 21.315830870166405 28.717672835230427 28.56488550945187 21.401610785151295 76-77 21.657733793723207 27.83949335097022 29.161042882135618 21.34172997317096 78-79 21.461448142260817 27.821894128290946 29.203367926307493 21.513289803140744 80-81 21.54466421986774 28.300158436458982 28.79689106472255 21.35828627895072 82-83 21.624234807725816 28.084245784516813 28.770751501704094 21.52076790605327 84-85 21.348552387888606 28.1516296880201 28.57979053014073 21.920027393950562 86-87 21.344945633611125 28.55758509115528 28.393021495386396 21.704447779847193 88-89 21.616625485934094 28.0748449312935 28.34778497487005 21.960744607902356 90-91 21.219308035714285 28.04125118213173 28.577165387182912 22.162275394971072 92-93 21.286868839621796 27.83306781945752 28.69692221182184 22.183141129098843 94-95 21.227009114224604 27.58445671416864 28.782855141449947 22.40567903015681 96-97 21.22118616153566 27.53796000239871 28.773425434483524 22.467428401582104 98-99 20.952214993635938 27.662965848732163 28.43916855662017 22.94565060101173 100-101 20.89086271140104 27.76909232125961 28.78092265191689 22.55912231542246 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2342.0 1 1609.0 2 1011.5 3 1170.0 4 972.5 5 949.0 6 1817.5 7 3710.5 8 5518.0 9 5987.0 10 5036.0 11 4019.0 12 3867.5 13 3951.5 14 3972.0 15 3927.0 16 3920.0 17 4012.0 18 4116.0 19 4262.0 20 4485.5 21 4756.0 22 5213.0 23 5781.0 24 6381.0 25 7207.0 26 8462.0 27 10124.0 28 11215.5 29 12588.5 30 14703.5 31 16779.0 32 19519.5 33 21597.5 34 22568.0 35 24422.5 36 28486.0 37 31650.0 38 32495.0 39 32495.5 40 32025.0 41 31966.5 42 31106.0 43 32913.5 44 36076.0 45 36778.0 46 36659.5 47 37926.0 48 41964.0 49 41371.5 50 38156.0 51 36766.0 52 34686.5 53 34399.5 54 35710.0 55 34229.5 56 30682.5 57 27833.0 58 25933.5 59 23925.5 60 19547.0 61 16096.5 62 13653.5 63 9554.5 64 6450.0 65 4859.0 66 3612.5 67 2770.0 68 2068.5 69 1509.0 70 1047.0 71 672.5 72 496.0 73 383.0 74 254.0 75 191.5 76 141.5 77 97.5 78 64.0 79 36.0 80 23.0 81 18.5 82 14.5 83 10.5 84 5.5 85 2.0 86 1.5 87 1.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017468858066831853 2 0.0 3 0.0 4 0.0 5 0.04015230063122545 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003563299406667194 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.125522870733057E-4 24-25 1.7381948325205824E-4 26-27 0.0013036461243904369 28-29 0.0011298266411383785 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 9.125522870733057E-4 42-43 0.012080454086018048 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 2.172743540650728E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.256425454472766E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.013471009952034513 92-93 0.0017381948325205824 94-95 0.0 96-97 0.0 98-99 0.0015209204784555096 100-101 0.019858875961547652 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1150619.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.776839361844665 #Duplication Level Percentage of deduplicated Percentage of total 1 67.3315154188337 26.782348728045154 2 14.669388923106133 11.670038534616324 3 6.455145873004799 7.702959013433591 4 3.392910108812631 5.398369614696756 5 2.050277892009113 4.077678717879399 6 1.346203235978793 3.2128625919554343 7 0.8868184068037913 2.4692383317392994 8 0.6271734965049731 1.995758353798781 9 0.48028414696045735 1.7193766825518035 >10 2.4902800757765378 17.53151052990406 >50 0.17008588710112058 4.660743809465242 >100 0.09241728117744438 7.197458945906329 >500 0.004631892133612292 1.2198577028307545 >1k 0.0022056629207677573 1.496045278241515 >5k 2.2056629207677574E-4 0.7002074457920203 >10k+ 4.411325841535515E-4 2.1655457191435206 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13628 1.1844059588795248 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11055 0.9607871936757519 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7981 0.6936266479173384 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2946 0.2560360988302818 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2423 0.21058230395986857 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2411 0.20953938706035619 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2225 0.1933741751179148 No Hit TCCCAGGACCGAGTGGGAGTAGGGGATTACTGAGTGGCATCCCCAAGCTG 1437 0.12488929871660386 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1254 0.1089848159990405 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1158 0.10064148080294172 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 8.690974162602912E-5 5 0.0 0.0 0.0 0.0 8.690974162602912E-5 6 0.0 0.0 0.0 0.0 8.690974162602912E-5 7 0.0 0.0 0.0 0.0 8.690974162602912E-5 8 0.0 0.0 0.0 0.0 8.690974162602912E-5 9 0.0 0.0 0.0 0.0 2.6072922487808736E-4 10-11 0.0 0.0 0.0 0.0 2.6072922487808736E-4 12-13 0.0 0.0 0.0 0.0 3.476389665041165E-4 14-15 0.0 0.0 0.0 0.0 5.214584497561747E-4 16-17 0.0 0.0 0.0 2.172743540650728E-4 6.083681913822038E-4 18-19 0.0 0.0 0.0 2.6072922487808736E-4 6.083681913822038E-4 20-21 0.0 0.0 0.0 3.0418409569110195E-4 6.083681913822038E-4 22-23 0.0 0.0 0.0 8.256425454472767E-4 6.95277933008233E-4 24-25 0.0 0.0 0.0 0.0014340107368294806 7.82187674634262E-4 26-27 0.0 0.0 0.0 0.00247692763634183 7.82187674634262E-4 28-29 0.0 0.0 0.0 0.004562761435366529 7.82187674634262E-4 30-31 0.0 0.0 0.0 0.009733891062115262 7.82187674634262E-4 32-33 0.0 0.0 0.0 0.015600298621872228 0.0010429168995123494 34-35 0.0 0.0 0.0 0.022813807176832644 0.0011298266411383785 36-37 0.0 0.0 0.0 0.03067913879398828 0.0011298266411383785 38-39 0.0 0.0 0.0 0.038240286315452815 0.0011298266411383785 40-41 0.0 0.0 0.0 0.049364733243584535 0.0011298266411383785 42-43 0.0 0.0 0.0 0.06205355552098479 0.0011298266411383785 44-45 0.0 0.0 0.0 0.074612013185946 0.0011298266411383785 46-47 0.0 0.0 0.0 0.089560488745623 0.0011298266411383785 48-49 0.0 0.0 0.0 0.10694243707082883 0.0011298266411383785 50-51 0.0 0.0 0.0 0.13084261601798683 0.0012167363827644078 52-53 0.0 0.0 0.0 0.17886024826636793 0.0014340107368294806 54-55 0.0 0.0 0.0 0.23613376799792113 0.001564375349268524 56-57 0.0 0.0 0.0 0.29153872828451466 0.001564375349268524 58-59 0.0 0.0 0.0 0.38661798562339056 0.001564375349268524 60-61 0.0 0.0 0.0 0.4899971232875522 0.001564375349268524 62-63 0.0 0.0 0.0 0.5707797281289462 0.0017381948325205824 64-65 0.0 0.0 0.0 0.644566098769445 0.0017381948325205824 66-67 0.0 0.0 0.0 0.8016554567584926 0.0017381948325205824 68-69 0.0 0.0 0.0 0.9663059622690047 0.0017816497033335968 70-71 0.0 0.0 0.0 1.0569528227849532 0.0019120143157726405 72-73 0.0 0.0 0.0 1.2284691978839217 0.002259653282276757 74-75 0.0 0.0 0.0 1.4576936414225734 0.002259653282276757 76-77 0.0 0.0 0.0 1.6887866444061848 0.002259653282276757 78-79 0.0 0.0 0.0 1.9405207110259781 0.002259653282276757 80-81 0.0 0.0 0.0 2.2199789852244747 0.0023031081530897717 82-83 0.0 0.0 0.0 2.449551067729631 0.002433472765528815 84-85 0.0 0.0 0.0 2.7120619423110517 0.002433472765528815 86-87 0.0 0.0 0.0 3.10124376531241 0.002433472765528815 88-89 0.0 0.0 0.0 3.6072322810591517 0.002433472765528815 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6435 0.0 38.923656 1 GTATCAA 10380 0.0 36.172733 1 TATCAAC 13430 0.0 27.88215 2 ATCAACG 13600 0.0 27.49868 3 TCAACGC 13675 0.0 27.313118 4 CAACGCA 13850 0.0 26.933693 5 TAAGGTG 450 0.0 26.399977 5 AACGCAG 14200 0.0 26.289585 6 GTAAGGT 400 0.0 26.135977 4 GGTAAGG 395 0.0 25.263773 3 GTGTACG 210 1.2569217E-9 24.895735 1 TGTACGC 135 1.3383664E-5 24.639977 2 ACGCAGA 15480 0.0 23.934797 7 TATCACG 100 0.0016496083 23.75998 2 CTATACG 120 1.7059522E-4 23.75998 4 CGCAGAG 15630 0.0 23.613924 8 ATATAGG 365 0.0 23.4345 2 AGTACTT 10010 0.0 23.3236 12-13 GGACCGA 675 0.0 23.219864 6 GAGTACT 9665 0.0 23.124048 12-13 >>END_MODULE