FastQCFastQC Report
Thu 26 May 2016
SRR937814_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937814_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155132
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105660.9147006575871848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80080.6932541042928427No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56920.49275753766669095No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25570.22135998310149835No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17140.14838131053420733No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16230.14050342298542504No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15980.138339168164331No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13640.1180817430388908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54400.037.5919151
GTATCAA87550.036.1778681
TATCAAC108050.029.42812
TCAACGC109400.029.110934
ATCAACG109300.029.048063
CAACGCA111000.028.6472455
AACGCAG114150.027.8838276
TAGGACG700.00836465827.1607425
GTACCGT1109.4677685E-525.9126646
GTACATG67350.025.2796081
ACGCAGA127200.024.8374447
CGCAGAG127200.024.6506968
TACATGG69750.024.1905082
TAAGGTG4150.024.0519835
GAGTACT69900.023.5463212-13
AGGTAAG3650.023.4390772
GCAGAGT130550.023.2893099
GGACCGA5750.023.1336256
ACATGGG69200.023.0090983
CATGGGA38550.022.317874
Produced by FastQC (version 0.11.5)