##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937814_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1155132 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.785203768919917 33.0 31.0 34.0 30.0 34.0 2 31.889900894443233 33.0 31.0 34.0 30.0 34.0 3 31.923716077469933 33.0 31.0 34.0 30.0 34.0 4 35.53063632554548 37.0 35.0 37.0 33.0 37.0 5 35.51211982699812 37.0 35.0 37.0 33.0 37.0 6 35.62304221508884 37.0 35.0 37.0 33.0 37.0 7 35.56902587756205 37.0 35.0 37.0 33.0 37.0 8 35.561051897099205 37.0 35.0 37.0 33.0 37.0 9 37.18231249761932 39.0 37.0 39.0 34.0 39.0 10-11 37.077998445199334 39.0 37.0 39.0 33.0 39.0 12-13 36.95607991121361 39.0 37.0 39.0 33.0 39.0 14-15 38.05973386591316 40.0 38.0 41.0 33.0 41.0 16-17 38.01833513399335 40.0 38.0 41.0 33.0 41.0 18-19 38.02343671545763 40.0 38.0 41.0 33.0 41.0 20-21 37.869219708223824 40.0 38.0 41.0 33.0 41.0 22-23 37.75356106488263 40.0 37.5 41.0 33.0 41.0 24-25 37.608838210697996 40.0 37.0 41.0 32.5 41.0 26-27 37.652310298736424 40.0 37.0 41.0 33.0 41.0 28-29 37.57777076559216 40.0 37.0 41.0 32.5 41.0 30-31 37.323959080001245 39.5 37.0 41.0 32.0 41.0 32-33 37.03048655911186 39.0 36.0 41.0 31.5 41.0 34-35 36.74874473220376 39.0 35.0 41.0 31.0 41.0 36-37 36.72765709892896 39.0 35.0 41.0 31.0 41.0 38-39 36.651097017483714 39.0 35.0 41.0 31.0 41.0 40-41 36.43171905894738 39.0 35.0 41.0 30.0 41.0 42-43 36.151655828078525 39.0 35.0 41.0 30.0 41.0 44-45 35.83757786988846 39.0 35.0 41.0 29.0 41.0 46-47 35.37123636086612 39.0 35.0 40.0 26.5 41.0 48-49 34.94947893400928 38.5 35.0 40.0 24.5 41.0 50-51 33.994109764078914 37.5 33.5 39.5 23.5 40.5 52-53 34.05833835440452 38.0 33.5 39.5 23.0 40.5 54-55 34.33676194582091 38.0 34.0 40.0 23.0 41.0 56-57 34.03752211868427 38.0 34.0 40.0 21.5 41.0 58-59 33.7920787407846 38.0 34.0 40.0 21.0 41.0 60-61 33.48056023034597 37.0 33.0 40.0 20.0 41.0 62-63 33.09321964935609 36.5 33.0 40.0 18.0 41.0 64-65 32.69977327266494 36.0 32.5 39.0 16.0 41.0 66-67 32.28582101439489 35.5 32.0 39.0 12.0 40.5 68-69 31.792937517097613 35.0 31.0 38.5 7.5 40.0 70-71 31.3415319634466 35.0 31.0 38.0 6.5 40.0 72-73 30.88053313387561 35.0 31.0 37.0 2.0 39.5 74-75 30.39848129910694 35.0 30.0 36.5 2.0 39.0 76-77 29.960734357631857 34.5 30.0 36.0 2.0 39.0 78-79 29.455396006690144 34.0 29.0 35.5 2.0 38.0 80-81 28.956877222689698 34.0 29.0 35.0 2.0 37.0 82-83 28.466541053316853 34.0 28.5 35.0 2.0 37.0 84-85 28.014329964021428 34.0 27.0 35.0 2.0 36.0 86-87 27.520809742955784 33.5 26.0 35.0 2.0 36.0 88-89 27.033494440462214 33.0 25.5 35.0 2.0 35.0 90-91 26.52716962217305 33.0 25.0 35.0 2.0 35.0 92-93 25.985466163174426 32.0 23.5 35.0 2.0 35.0 94-95 25.366611781164405 32.0 20.5 34.5 2.0 35.0 96-97 24.618352274891528 32.0 14.0 34.0 2.0 35.0 98-99 23.72460506678025 31.0 2.0 34.0 2.0 35.0 100-101 21.250460120574964 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10012066001331021 1101 2 0.4255449245928382 1101 3 0.24568326495087334 1101 4 0.21460698573385173 1101 5 0.2693284435402461 1101 6 0.2676263265933656 1101 7 0.31900706248500654 1101 8 0.2590788861772779 1101 9 0.4478653120099594 1101 10-11 0.1797698752863539 1101 12-13 0.3304888638444723 1101 14-15 0.5055137340933058 1101 16-17 0.5774248383792298 1101 18-19 0.5349167215540831 1101 20-21 0.6631735188985672 1101 22-23 0.5905840601726453 1101 24-25 0.5397940511459254 1101 26-27 0.5974307667968546 1101 28-29 0.5431438222170115 1101 30-31 0.5511210390482759 1101 32-33 0.43601138154133423 1101 34-35 0.6369516609315795 1101 36-37 0.44992504693239965 1101 38-39 0.5037609705105552 1101 40-41 0.7476899481467711 1101 42-43 0.5976787020693664 1101 44-45 0.7169024836802862 1101 46-47 0.8039561763710452 1101 48-49 1.0055302059632467 1101 50-51 0.7103344511734164 1101 52-53 0.6966977635295635 1101 54-55 0.8068201699866577 1101 56-57 0.8851699669524749 1101 58-59 0.7689844282310148 1101 60-61 0.8706877222476734 1101 62-63 0.7322906455539098 1101 64-65 0.8164261833024398 1101 66-67 1.087763127375105 1101 68-69 1.0840326312960151 1101 70-71 0.966527218666279 1101 72-73 0.9278566226172416 1101 74-75 0.857844348887145 1101 76-77 0.993930800496063 1101 78-79 1.0822609721782044 1101 80-81 1.2388418338756786 1101 82-83 1.3193456927392972 1101 84-85 1.1696614728745836 1101 86-87 1.359275590325467 1101 88-89 1.264143700389738 1101 90-91 1.3849205921289887 1101 92-93 1.5020678731025008 1101 94-95 1.7168689179289096 1101 96-97 1.8718448477238248 1101 98-99 1.9036777005252752 1101 100-101 2.2713739595106652 1102 1 0.3567210824403446 1102 2 0.4060760785866897 1102 3 0.44256186518128615 1102 4 0.29708826453569515 1102 5 0.2391093095156691 1102 6 0.38369415493437486 1102 7 0.32483943575689267 1102 8 0.221522482567444 1102 9 0.3384171073248581 1102 10-11 0.3095695824676099 1102 12-13 0.3433242853114393 1102 14-15 0.5707907118198747 1102 16-17 0.6899464397617194 1102 18-19 0.5362314056938686 1102 20-21 0.3562916784607708 1102 22-23 0.4323761348654287 1102 24-25 0.3511809799093655 1102 26-27 0.3409629528567706 1102 28-29 0.38536492436755054 1102 30-31 0.4325564509146389 1102 32-33 0.31407157739386804 1102 34-35 0.557355966807151 1102 36-37 0.46540843786941366 1102 38-39 0.5832486577225424 1102 40-41 0.601652601756598 1102 42-43 0.8100664948881118 1102 44-45 0.7146817415527948 1102 46-47 0.7523642744893166 1102 48-49 0.7503422739356012 1102 50-51 0.534648007240996 1102 52-53 0.4390820761255654 1102 54-55 0.5938881423368869 1102 56-57 0.7739432484424853 1102 58-59 0.8971640317256444 1102 60-61 0.9527327731309256 1102 62-63 0.9252743965139771 1102 64-65 1.0286201637171857 1102 66-67 0.9446353671984511 1102 68-69 0.9701687440072355 1102 70-71 0.9231576430134396 1102 72-73 0.8405918376710027 1102 74-75 0.882936754801122 1102 76-77 1.1707015138217152 1102 78-79 1.0533985249815814 1102 80-81 1.228032142055401 1102 82-83 1.2170259442753952 1102 84-85 1.303338028174121 1102 86-87 1.3373735885973552 1102 88-89 1.4076656857968004 1102 90-91 1.3730008292641678 1102 92-93 1.4099067747922085 1102 94-95 1.3216386231900437 1102 96-97 1.5675887753351923 1102 98-99 1.8697521292887167 1102 100-101 2.003324804364734 1103 1 0.22477116130469454 1103 2 0.27802254940688087 1103 3 0.20640009467655673 1103 4 0.18275959734326364 1103 5 0.2352326336481667 1103 6 0.22996042070724343 1103 7 0.2741197985329151 1103 8 0.27383172941286915 1103 9 0.2794832548011996 1103 10-11 0.44808372876099156 1103 12-13 0.28864048207800863 1103 14-15 0.4454998995170385 1103 16-17 0.4099434823484671 1103 18-19 0.4630144794320401 1103 20-21 0.4930954540916943 1103 22-23 0.3378880138086444 1103 24-25 0.35269049292198673 1103 26-27 0.2946461646075633 1103 28-29 0.3833883865126637 1103 30-31 0.41565606645091435 1103 32-33 0.30911791020143653 1103 34-35 0.20699738045068017 1103 36-37 0.3212986192574263 1103 38-39 0.4189926936058228 1103 40-41 0.4927951490752065 1103 42-43 0.4650115362918896 1103 44-45 0.3894488452594089 1103 46-47 0.4511357157353757 1103 48-49 0.6204650065854409 1103 50-51 0.17732348042711976 1103 52-53 0.25597383480064906 1103 54-55 0.3011633789299495 1103 56-57 0.7583207129535268 1103 58-59 0.676475693791879 1103 60-61 0.5046711110646811 1103 62-63 0.5494204434382581 1103 64-65 0.5786029121398997 1103 66-67 0.684231433838832 1103 68-69 0.7967743729504484 1103 70-71 0.904459397745299 1103 72-73 0.8288903392482929 1103 74-75 0.947499550542446 1103 76-77 0.8942412910299176 1103 78-79 0.9743209914642321 1103 80-81 1.082065314374013 1103 82-83 1.2228101516886412 1103 84-85 1.4345239508870549 1103 86-87 1.351060250663604 1103 88-89 1.251814936585447 1103 90-91 1.279466473980257 1103 92-93 1.4626768852023027 1103 94-95 1.3564436803666986 1103 96-97 1.452529380619108 1103 98-99 1.5229621549196288 1103 100-101 1.8503279589388661 1104 1 0.2324448562823065 1104 2 0.3961937273151186 1104 3 0.2807310011940025 1104 4 0.2640572132187131 1104 5 0.37762755996980246 1104 6 0.31101377913964257 1104 7 0.3860763794444466 1104 8 0.3760588415592707 1104 9 0.5614465975610088 1104 10-11 0.4868200224085797 1104 12-13 0.34206087420790254 1104 14-15 0.6619195151804576 1104 16-17 0.6705450433039601 1104 18-19 0.8299271226557252 1104 20-21 0.7511695593644987 1104 22-23 0.7317889555965778 1104 24-25 0.7283469795687623 1104 26-27 0.6026103898458715 1104 28-29 0.5606132781368487 1104 30-31 0.6657978933533286 1104 32-33 0.6945708394283869 1104 34-35 0.7266271549315846 1104 36-37 0.8054104246995664 1104 38-39 0.8108822720504207 1104 40-41 0.9031895567974715 1104 42-43 0.824234367844511 1104 44-45 0.8196044680514234 1104 46-47 0.7827950618417248 1104 48-49 0.7989720707112653 1104 50-51 0.7913720081492599 1104 52-53 0.8666521828467282 1104 54-55 0.9471408671325392 1104 56-57 1.1218180497594687 1104 58-59 1.3043261996411744 1104 60-61 1.3277742836895143 1104 62-63 1.2669847024858143 1104 64-65 1.2006746880132795 1104 66-67 1.1861278314355133 1104 68-69 1.3214655054740767 1104 70-71 1.1503012908335961 1104 72-73 1.1273453571987098 1104 74-75 1.2073726369537106 1104 76-77 1.243913011891653 1104 78-79 1.254966346848068 1104 80-81 1.453744917373296 1104 82-83 1.3976082274270745 1104 84-85 1.3322535027426952 1104 86-87 1.314174153424787 1104 88-89 1.3057313647250481 1104 90-91 1.3921364871887825 1104 92-93 1.535499985509741 1104 94-95 1.3664292254834116 1104 96-97 1.5729429278102138 1104 98-99 1.801131628039851 1104 100-101 1.9921985555000639 1105 1 0.16824451994034462 1105 2 0.19552920358669112 1105 3 0.19842124018128615 1105 4 0.056853889535695146 1105 5 0.07680462201567195 1105 6 0.18974884243437629 1105 7 0.17151912325689267 1105 8 0.297694357567444 1105 9 0.2513077323248609 1105 10-11 0.2728508324676113 1105 12-13 0.346839910311445 1105 14-15 0.24002899306987757 1105 16-17 0.36074722101172085 1105 18-19 0.3400399994438672 1105 20-21 0.5084401159607737 1105 22-23 0.5063019161154259 1105 24-25 0.4756926986593655 1105 26-27 0.40941998410676916 1105 28-29 0.2717907056175477 1105 30-31 0.4179080134146389 1105 32-33 0.42002860864386804 1105 34-35 0.5045239355571525 1105 36-37 0.3774201566194151 1105 38-39 0.412447876472541 1105 40-41 0.42782447675659796 1105 42-43 0.49190243238810893 1105 44-45 0.43577549155278916 1105 46-47 0.32111427448931806 1105 48-49 0.16040086768560258 1105 50-51 -0.07834027400900112 1105 52-53 0.07609379487556822 1105 54-55 0.1393959548368926 1105 56-57 -0.07674034530751328 1105 58-59 0.17636325047563872 1105 60-61 0.18691246063092848 1105 62-63 0.09363377151397145 1105 64-65 0.1706123512171871 1105 66-67 0.2209048984484525 1105 68-69 0.28989530650722983 1105 70-71 0.29688811176343677 1105 72-73 0.1558262126710055 1105 74-75 -0.07673121394887872 1105 76-77 -0.09892739242828341 1105 78-79 0.2632618062315828 1105 80-81 0.266606360805401 1105 82-83 0.2739595380253945 1105 84-85 0.4874200594241209 1105 86-87 0.24059624484735664 1105 88-89 0.19165006079680325 1105 90-91 0.3310086417641678 1105 92-93 0.48978958729221134 1105 94-95 0.43345502944004366 1105 96-97 0.5128036190851937 1105 98-99 0.8171154105387153 1105 100-101 1.003324804364734 1106 1 0.2687842544833572 1106 2 0.3682484576189502 1106 3 0.33784916356838224 1106 4 0.13104323765397652 1106 5 0.29820709177373317 1106 6 0.14166626514943204 1106 7 0.11916067231603478 1106 8 0.10439378633088836 1106 9 0.2157608573248595 1106 10-11 0.004424253166533276 1106 12-13 -0.026807603129412882 1106 14-15 0.19399299676612003 1106 16-17 0.15999012255741718 1106 18-19 0.05050811940354549 1106 20-21 0.09666046206292123 1106 22-23 -0.01832531002704485 1106 24-25 0.17312738447925824 1106 26-27 0.12031863162021494 1106 28-29 0.41249866092668697 1106 30-31 0.371320731828618 1106 32-33 0.37164886234010197 1106 34-35 0.3420207853555368 1106 36-37 0.4301093787296324 1106 38-39 0.5584460703569505 1106 40-41 0.39431053186412157 1106 42-43 0.5859275499956382 1106 44-45 0.34224810580010256 1106 46-47 0.687289509704371 1106 48-49 0.8595240615700064 1106 50-51 0.9615633298216508 1106 52-53 0.9786486083836294 1106 54-55 0.7996005079282895 1106 56-57 0.9585954661844198 1106 58-59 1.0012424927471457 1106 60-61 0.972915064797597 1106 62-63 1.0345947930193446 1106 64-65 1.0204968438247164 1106 66-67 1.2337692717683453 1106 68-69 1.1491631996523886 1106 70-71 1.0371109360241917 1106 72-73 0.9617275903591782 1106 74-75 1.0202099402849925 1106 76-77 1.2290306468862333 1106 78-79 1.2632901580460967 1106 80-81 1.0661075788833578 1106 82-83 0.985935554490446 1106 84-85 1.0185093991418626 1106 86-87 0.8642679098339165 1106 88-89 1.291486250312932 1106 90-91 1.629851467705027 1106 92-93 1.4811769360825338 1106 94-95 1.7111085492653118 1106 96-97 1.6217680428083128 1106 98-99 1.9848604962241971 1106 100-101 1.4753678151174192 1107 1 0.4009062090226223 1107 2 0.5037323285866897 1107 3 0.5788454885990042 1107 4 0.4826697518774665 1107 5 0.3529641354650366 1107 6 0.3098709745546273 1107 7 0.4595308914530918 1107 8 0.3226597452256712 1107 9 0.5853811104894149 1107 10-11 0.4377984194929283 1107 12-13 0.567360084362079 1107 14-15 0.7532076421996194 1107 16-17 0.6593095726731093 1107 18-19 0.6311532806938658 1107 20-21 0.7564944078911537 1107 22-23 0.6961567915109939 1107 24-25 0.8284901175675898 1107 26-27 0.6866858563377818 1107 28-29 0.7610967281650147 1107 30-31 0.8564439113576796 1107 32-33 0.9485850267609592 1107 34-35 1.0427149462375311 1107 36-37 0.9225484695149859 1107 38-39 0.9676879377858327 1107 40-41 1.0942356713768504 1107 42-43 1.2510425629260808 1107 44-45 1.5301139013629168 1107 46-47 1.5662537127804583 1107 48-49 1.3649684606444623 1107 50-51 1.2825514882536524 1107 52-53 1.1891018546065837 1107 54-55 1.2539356106913218 1107 56-57 1.1873827262905934 1107 58-59 1.2267971409028533 1107 60-61 1.3404256133207966 1107 62-63 1.2829138348051075 1107 64-65 1.3887764137171814 1107 66-67 1.4790054225781972 1107 68-69 1.6404861886274809 1107 70-71 1.404635095545089 1107 72-73 1.263653546531767 1107 74-75 1.58094901745935 1107 76-77 1.6955680090748793 1107 78-79 1.4102072670701915 1107 80-81 1.4705311531313505 1107 82-83 1.8750263398450144 1107 84-85 1.8710002117184246 1107 86-87 1.6105589129644464 1107 88-89 1.5636585655436406 1107 90-91 1.8084685903401159 1107 92-93 2.0144558254251237 1107 94-95 2.0344105773039693 1107 96-97 2.2111854921073437 1107 98-99 2.3480699694785905 1107 100-101 2.396362779048278 1108 1 0.17791874253886775 1108 2 0.33156484090196514 1108 3 0.4478920683832577 1108 4 0.2924315527130332 1108 5 0.34907313340729473 1108 6 0.2501465872003834 1108 7 0.2827366032445795 1108 8 0.3752132153260632 1108 9 0.36502194106870434 1108 10-11 0.4675764328370704 1108 12-13 0.4452658647449397 1108 14-15 0.5887940985193794 1108 16-17 0.6022540136533507 1108 18-19 0.6215473693638174 1108 20-21 0.5874743293228377 1108 22-23 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-0.9393384792454853 2316 52-53 -1.2618892076582213 2316 54-55 -1.0812807765820267 2316 56-57 -0.7306809069629168 2316 58-59 -0.7423313230040876 2316 60-61 -0.7534644397069101 2316 62-63 -0.6166702825400847 2316 64-65 -0.8129972349314585 2316 66-67 -0.7847037375312773 2316 68-69 -0.9854161377495281 2316 70-71 -0.9942198907703457 2316 72-73 -1.0388000542208893 2316 74-75 -0.9351164546583384 2316 76-77 -1.0737347192863886 2316 78-79 -0.9927691270454453 2316 80-81 -0.9456904086202762 2316 82-83 -0.9977755759948757 2316 84-85 -0.9215352826366896 2316 86-87 -0.6834562255918328 2316 88-89 -0.7240488412302213 2316 90-91 -0.8071617551952919 2316 92-93 -0.7629817387213009 2316 94-95 -0.5022106166748195 2316 96-97 -0.6010450929080484 2316 98-99 -0.6997896781437198 2316 100-101 -0.4789387091487818 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4396.0 3 775.0 4 733.0 5 799.0 6 940.0 7 1164.0 8 1440.0 9 1729.0 10 2309.0 11 3093.0 12 4505.0 13 5923.0 14 6989.0 15 9175.0 16 12025.0 17 16127.0 18 13484.0 19 8847.0 20 8533.0 21 8773.0 22 9708.0 23 10371.0 24 11425.0 25 12858.0 26 14764.0 27 17444.0 28 20759.0 29 25029.0 30 30377.0 31 37896.0 32 47306.0 33 62222.0 34 84302.0 35 121594.0 36 167997.0 37 210182.0 38 143247.0 39 15891.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.86578417066222 19.25126815370718 15.342835800152873 21.54011187547773 2 15.247049059067436 20.90566712622283 40.2717921648568 23.575491649852932 3 18.586115864049468 25.971096926085952 30.54763399162615 24.895153218238438 4 12.277870333312787 16.91783991297561 37.418751047218365 33.385538706493236 5 12.867943268322499 38.148198310829585 33.97441240206701 15.009446018780908 6 30.367798501182502 36.87042464182287 17.42501140857265 15.336765448421975 7 27.02845721701534 34.62395944300055 20.719706301810977 17.627877038173132 8 26.325021361867872 33.84517959150036 20.226847855788957 19.602951190842806 9 26.75026721636891 16.45974749788309 20.824570718638515 35.965414567109484 10-11 24.28728089201373 27.272589248222324 26.882697626641033 21.557432233122917 12-13 25.785151929208144 25.04452686143102 27.860845424772812 21.309475784588024 14-15 22.939398487499794 25.852050169130386 27.083158014368493 24.12539332900133 16-17 20.85965112400803 29.345433553388595 27.70601392329135 22.088901399312032 18-19 20.693187875696527 27.219480289093585 31.413271708151324 20.674060127058564 20-21 21.654173360025812 26.086189070192667 31.75644730952984 20.50319026025168 22-23 21.64057335880207 26.05830570461473 31.50437288447292 20.796748052110278 24-25 21.281894692646805 26.30285276089585 31.580148129128073 20.83510441732927 26-27 20.61799195337887 26.69867329642552 31.636423885869142 21.046910864326474 28-29 20.631354022480934 26.668925199895373 31.911686434616115 20.788034343007578 30-31 20.722668729046724 26.71283887973898 31.39878209112672 21.165710300087575 32-33 20.34991311167774 26.875213099791868 31.443031539202988 21.33184224932741 34-35 21.16498646862582 26.7502623208378 31.229084277970298 20.855666932566088 36-37 21.266256930080942 26.40380898023124 31.04090759068035 21.289026499007463 38-39 20.7602244081359 26.846625096434177 31.041202926134996 21.351947569294925 40-41 20.93036668535674 26.702650477854434 31.292516121814177 21.07446671497465 42-43 20.753504239697413 26.864124539674815 30.72779845834705 21.654572762280726 44-45 20.811513896635507 27.199819921879403 30.544450063452384 21.444216118032706 46-47 21.21781332269299 27.331991724819904 29.948735519519104 21.501459432968005 48-49 21.043029092656823 27.559857096009065 29.51884420584699 21.878269605487123 50-51 21.569204943969357 27.823209069004328 29.17965590007959 21.427930086946713 52-53 21.472068180694112 27.789993119108836 29.160054040592918 21.577884659604134 54-55 21.235138590827 28.028058201728285 29.19025379548203 21.546549411962687 56-57 21.408747900099822 28.264070957328986 28.79762122614223 21.52955991642896 58-59 21.09785167675543 27.600799815787386 29.17632069993855 22.12502780751863 60-61 21.02248041646396 27.991730384160775 29.33376519875782 21.652024000617445 62-63 21.124925904421367 28.044835034080712 29.54027568975558 21.289963371742342 64-65 21.04943649018221 27.93240397177833 29.35051216930575 21.667647368733714 66-67 20.837384082096715 27.973668759902836 29.286544980872947 21.9024021771275 68-69 21.154368178447687 28.127279271153586 29.227313527975685 21.491039022423042 70-71 21.192955859861314 27.981121267324262 28.94470156589929 21.881221306915133 72-73 21.044153057689112 28.148891414517003 28.597000091495 22.209955436298884 74-75 20.921959055653574 28.63077053852866 28.59867705454678 21.84859335127099 76-77 21.144812596381854 27.99414726887003 28.897903320652997 21.963136814095126 78-79 21.13139909612625 27.879270708063352 28.945817448033328 22.04351274777707 80-81 21.16176688687272 28.306538272401564 28.560698900530873 21.970995940194847 82-83 21.183267915140327 28.044048779874466 28.6975225487905 22.075160756194713 84-85 21.081881182624137 28.048259153405386 28.523835620239467 22.34602404373101 86-87 21.23855665329207 28.43716641186423 28.315069360317246 22.009207574526464 88-89 21.253520363364373 28.051859640754913 28.262680354258585 22.43193964162213 90-91 20.95098960054326 27.855947880430964 28.567934704837032 22.62512781418874 92-93 21.090431171182814 27.77400294075944 28.59483125322228 22.54073463483547 94-95 20.955117640095587 27.444369141139063 28.82480447518438 22.77570874358097 96-97 20.86772057267546 27.304422034198172 28.823401361960165 23.0044560311662 98-99 20.756215203059313 27.389523643488513 28.775416565837396 23.078844587614782 100-101 21.059151534072186 26.91620271606223 29.16835770158003 22.85628804828555 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2570.0 1 1659.0 2 865.0 3 981.5 4 845.5 5 903.5 6 1667.5 7 3228.0 8 4817.5 9 5326.0 10 4433.0 11 3581.5 12 3561.0 13 3576.0 14 3539.5 15 3394.5 16 3302.0 17 3379.0 18 3453.5 19 3587.5 20 3836.5 21 4213.0 22 4746.5 23 5449.5 24 6157.0 25 6998.0 26 8337.0 27 9958.5 28 10983.5 29 12257.5 30 14436.5 31 16735.0 32 20123.5 33 22503.0 34 23218.0 35 25012.0 36 28906.5 37 32244.0 38 33089.0 39 33089.0 40 32688.5 41 32499.5 42 31947.0 43 33886.5 44 36924.0 45 38201.5 46 38287.5 47 38899.0 48 42299.0 49 41904.5 50 39016.5 51 37094.5 52 34809.5 53 34663.5 54 35503.0 55 33857.5 56 30735.0 57 27977.0 58 26092.0 59 23816.5 60 19837.5 61 16369.5 62 13397.5 63 9625.5 64 6665.5 65 5080.0 66 3641.0 67 2647.5 68 2002.0 69 1468.5 70 1061.0 71 709.5 72 478.0 73 381.0 74 288.0 75 196.0 76 141.0 77 106.5 78 77.5 79 54.0 80 32.0 81 22.0 82 17.0 83 13.0 84 7.5 85 4.5 86 3.5 87 1.0 88 3.0 89 4.0 90 2.5 91 3.0 92 2.0 93 1.5 94 2.5 95 2.0 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33346838283416963 2 0.2853353556130382 3 0.27815002960700597 4 0.28317110079194413 5 0.2877593210126635 6 0.21555978018096633 7 0.2063833397395276 8 0.20577734838962128 9 0.21711804365215404 10-11 0.216771762880779 12-13 0.2127029638171222 14-15 0.21391494651693488 16-17 0.20045328152972997 18-19 0.20400265943632417 20-21 0.19460979351277602 22-23 0.20309367241146467 24-25 0.20378623395421475 26-27 0.2034399531828397 28-29 0.21352538064913795 30-31 0.2093700113926374 32-33 0.21668519268793524 34-35 0.21127455563520015 36-37 0.19716361420166698 38-39 0.18556320836060294 40-41 0.18296610257529008 42-43 0.19725018439451075 44-45 0.19902487334780786 46-47 0.19798603103368273 48-49 0.2138283763240911 50-51 0.20902373062126234 52-53 0.23070956392862463 54-55 0.2003234262404643 56-57 0.18188397516474308 58-59 0.18322581315382136 60-61 0.17270753472330436 62-63 0.17837788235457072 64-65 0.17716589965475807 66-67 0.17772860590824252 68-69 0.1798062905364928 70-71 0.18080184775419605 72-73 0.17863759293310202 74-75 0.19478293389846357 76-77 0.18751103769958757 78-79 0.19093056031691616 80-81 0.1836153790216183 82-83 0.16539235342800648 84-85 0.164483366403147 86-87 0.16889844623817885 88-89 0.1748285044479765 90-91 0.18101827323630545 92-93 0.17560763618357036 94-95 0.174655364062289 96-97 0.18075856265777418 98-99 0.18984843290636913 100-101 0.17244782414477308 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1155132.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.30387386155303 #Duplication Level Percentage of deduplicated Percentage of total 1 68.73090689188376 29.075836155443962 2 14.468613812416686 12.241568273439988 3 6.112676540310414 7.757696919532983 4 3.19968953177133 5.414370493927441 5 1.8978636109977927 4.014349140304108 6 1.2427954961921461 3.1545038343971257 7 0.831529285959034 2.4623837017778767 8 0.6140948973927479 2.078287446266093 9 0.44524042885260634 1.695185544621999 >10 2.209199886164152 16.611760336931294 >50 0.1594348608973772 4.63535806244743 >100 0.08155429414968735 6.563852965166669 >500 0.004542264073241806 1.3086653885343076 >1k 0.0012387992927023108 0.866708045813651 >5k 4.129330975674369E-4 1.1966038725835955 >10k+ 2.0646654878371846E-4 0.9228698188115527 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10566 0.9147006575871848 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8008 0.6932541042928427 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5692 0.49275753766669095 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2557 0.22135998310149835 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1714 0.14838131053420733 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1623 0.14050342298542504 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1598 0.138339168164331 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1364 0.1180817430388908 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.657019284376158E-5 2 0.0 0.0 0.0 0.0 8.657019284376158E-5 3 0.0 0.0 0.0 0.0 8.657019284376158E-5 4 0.0 0.0 0.0 0.0 8.657019284376158E-5 5 0.0 0.0 0.0 0.0 8.657019284376158E-5 6 0.0 0.0 0.0 0.0 8.657019284376158E-5 7 0.0 0.0 0.0 0.0 8.657019284376158E-5 8 0.0 0.0 0.0 0.0 8.657019284376158E-5 9 0.0 0.0 0.0 0.0 1.7314038568752316E-4 10-11 0.0 0.0 0.0 0.0 1.7314038568752316E-4 12-13 0.0 0.0 0.0 4.328509642188079E-5 2.1642548210940396E-4 14-15 0.0 0.0 0.0 1.2985528926564237E-4 3.4628077137504633E-4 16-17 0.0 0.0 0.0 3.895658677969271E-4 4.328509642188079E-4 18-19 0.0 0.0 0.0 5.194211570625695E-4 6.059913499063311E-4 20-21 0.0 0.0 0.0 7.358466391719734E-4 6.059913499063311E-4 22-23 0.0 0.0 0.0 0.001255267796234543 7.358466391719734E-4 24-25 0.0 0.0 0.0 0.002250825013937801 8.657019284376158E-4 26-27 0.0 0.0 0.0 0.0030732418459535364 8.657019284376158E-4 28-29 0.0 0.0 0.0 0.005800202920532026 8.657019284376158E-4 30-31 0.0 0.0 0.0 0.011124269780423364 9.089870248594966E-4 32-33 0.0 0.0 0.0 0.019348438100580713 0.0011254125069689006 34-35 0.0 0.0 0.0 0.02627405352808164 0.0012119826998126621 36-37 0.0 0.0 0.0 0.0331130987627388 0.0012119826998126621 38-39 0.0 0.0 0.0 0.04012528438308349 0.0012119826998126621 40-41 0.0 0.0 0.0 0.050946558488553687 0.0012119826998126621 42-43 0.0 0.0 0.0 0.06536049559703999 0.001255267796234543 44-45 0.0 0.0 0.0 0.08007742838047946 0.0013418379890783045 46-47 0.0 0.0 0.0 0.09886316022757571 0.0015149783747658277 48-49 0.0 0.0 0.0 0.11834145361742207 0.00164483366403147 50-51 0.0 0.0 0.0 0.14002728692478436 0.00164483366403147 52-53 0.0 0.0 0.0 0.18647219538546245 0.0017746889532971124 54-55 0.0 0.0 0.0 0.24884601932939265 0.0019478293389846356 56-57 0.0 0.0 0.0 0.31169597933396354 0.0020343995318283974 58-59 0.0 0.0 0.0 0.4060142044372418 0.002077684628250278 60-61 0.0 0.0 0.0 0.5247885090188826 0.002077684628250278 62-63 0.0 0.0 0.0 0.6126139696588788 0.0021642548210940396 64-65 0.0 0.0 0.0 0.6856792124190136 0.0021642548210940396 66-67 0.0 0.0 0.0 0.8494700172794105 0.0021642548210940396 68-69 0.0 0.0 0.0 1.0155549322501671 0.002250825013937801 70-71 0.0 0.0 0.0 1.105284937132726 0.002510535592469086 72-73 0.0 0.0 0.0 1.2853942233441717 0.002856816363844132 74-75 0.0 0.0 0.0 1.5274011974389072 0.002856816363844132 76-77 0.0 0.0 0.0 1.76611850420558 0.002856816363844132 78-79 0.0 0.0 0.0 2.028339618329334 0.002856816363844132 80-81 0.0 0.0 0.0 2.314454105677966 0.002943386556687894 82-83 0.0 0.0 0.0 2.562780703850296 0.0030299567495316554 84-85 0.0 0.0 0.0 2.8303691699303632 0.0030299567495316554 86-87 0.0 0.0 0.0 3.2270337935404783 0.0030299567495316554 88-89 0.0 0.0 0.0 3.7300066139627335 0.0030299567495316554 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5440 0.0 37.591915 1 GTATCAA 8755 0.0 36.177868 1 TATCAAC 10805 0.0 29.4281 2 TCAACGC 10940 0.0 29.11093 4 ATCAACG 10930 0.0 29.04806 3 CAACGCA 11100 0.0 28.647245 5 AACGCAG 11415 0.0 27.883827 6 TAGGACG 70 0.008364658 27.160742 5 GTACCGT 110 9.4677685E-5 25.912664 6 GTACATG 6735 0.0 25.279608 1 ACGCAGA 12720 0.0 24.837444 7 CGCAGAG 12720 0.0 24.650696 8 TACATGG 6975 0.0 24.190508 2 TAAGGTG 415 0.0 24.051983 5 GAGTACT 6990 0.0 23.54632 12-13 AGGTAAG 365 0.0 23.439077 2 GCAGAGT 13055 0.0 23.289309 9 GGACCGA 575 0.0 23.133625 6 ACATGGG 6920 0.0 23.009098 3 CATGGGA 3855 0.0 22.31787 4 >>END_MODULE