FastQCFastQC Report
Thu 26 May 2016
SRR937814_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937814_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155132
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134571.1649750850984997No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108350.9379880394621567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79680.6897912965790923No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29610.25633434101037805No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24760.21434779748115365No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23260.20136226855458944No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20680.17902715880089895No Hit
TCCCAGGACCGAGTGGGAGTAGGGGATTACTGAGTGGCATCCCCAAGCTG13460.11652347956770308No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12140.10509621411232656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66500.040.655641
GTATCAA104800.034.1853681
GTAAGGT3950.028.8648364
TAAGGTG4550.028.1907965
TATCAAC132000.027.2803672
ATCAACG133850.026.9388073
TCAACGC134500.026.843944
CAACGCA136000.026.5478675
AACGCAG139450.025.920656
GGTAAGG4150.025.184283
GAGTACT94350.024.16666212-13
ACGCAGA153250.023.5265717
CGCAGAG154350.023.1126988
GTACTTT99650.022.64102414-15
GTACATG65400.022.4497181
AGTACTT98450.022.0987212-13
GCAGAGT158750.022.0830969
GTACTAA1302.9352724E-421.9299721
TACATGG66700.021.082442
GGTGTGC5950.020.7574378
Produced by FastQC (version 0.11.5)