##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937814_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1155132 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.153452592430995 33.0 31.0 34.0 31.0 34.0 2 32.339350827437904 34.0 31.0 34.0 31.0 34.0 3 32.41549970046713 34.0 31.0 34.0 31.0 34.0 4 36.08319049251514 37.0 35.0 37.0 35.0 37.0 5 36.00107866460284 37.0 35.0 37.0 35.0 37.0 6 36.04710543903207 37.0 35.0 37.0 35.0 37.0 7 35.99306919036093 37.0 35.0 37.0 35.0 37.0 8 35.97512405508635 37.0 35.0 37.0 35.0 37.0 9 37.61278970715035 39.0 37.0 39.0 35.0 39.0 10-11 37.55321383183913 39.0 37.0 39.0 35.0 39.0 12-13 37.46049152824093 39.0 37.0 39.0 35.0 39.0 14-15 38.59044550752641 40.0 38.0 41.0 34.0 41.0 16-17 38.56819610226364 40.0 38.0 41.0 34.0 41.0 18-19 38.626669506168994 40.0 38.0 41.0 34.0 41.0 20-21 38.53491765443257 40.0 38.0 41.0 34.0 41.0 22-23 38.380308051374215 40.0 38.0 41.0 34.0 41.0 24-25 38.41900492757538 40.0 38.0 41.0 34.0 41.0 26-27 38.25947207765 40.0 38.0 41.0 34.0 41.0 28-29 38.083980445524844 40.0 37.0 41.0 33.0 41.0 30-31 38.01346209783817 40.0 37.0 41.0 34.0 41.0 32-33 37.839452547414496 40.0 36.5 41.0 33.0 41.0 34-35 37.66477034659242 40.0 36.0 41.0 33.0 41.0 36-37 37.453223960551696 40.0 36.0 41.0 33.0 41.0 38-39 37.26443904246441 40.0 35.5 41.0 33.0 41.0 40-41 37.023528912713005 39.5 35.0 41.0 32.0 41.0 42-43 36.72355150753334 39.0 35.0 41.0 31.0 41.0 44-45 36.284892549076645 39.0 35.0 41.0 30.5 41.0 46-47 35.760606147176254 39.0 35.0 41.0 28.5 41.0 48-49 35.2866676708809 39.0 35.0 40.0 26.5 41.0 50-51 34.944253557169226 39.0 35.0 40.0 25.5 41.0 52-53 34.66020896313149 38.0 34.5 40.0 24.0 41.0 54-55 34.40419449898367 38.0 34.0 40.0 23.5 41.0 56-57 34.10434348628554 38.0 34.0 40.0 23.0 41.0 58-59 33.79774908841587 37.5 33.5 40.0 22.0 41.0 60-61 33.42107179092952 37.0 33.0 40.0 20.5 41.0 62-63 33.0295957518275 36.5 33.0 39.5 19.5 41.0 64-65 32.59691965939824 36.0 32.5 39.0 17.5 40.5 66-67 32.14065621937579 35.0 32.0 39.0 14.0 40.0 68-69 31.68498924798205 35.0 31.0 38.0 9.0 40.0 70-71 31.26047759043988 35.0 31.0 37.5 7.5 40.0 72-73 30.770672529200127 35.0 30.5 37.0 4.5 39.0 74-75 30.271720461384497 34.0 30.0 36.0 2.0 39.0 76-77 29.06888173819096 33.5 28.5 35.0 2.0 37.0 78-79 29.650608328745115 34.0 30.0 35.0 2.0 37.0 80-81 29.46433784190898 34.0 29.5 35.0 2.0 37.0 82-83 29.11349655277492 34.0 29.0 35.0 2.0 36.5 84-85 28.72961618239301 34.0 29.0 35.0 2.0 36.0 86-87 28.326444510237792 34.0 29.0 35.0 2.0 36.0 88-89 27.953715246396083 34.0 28.0 35.0 2.0 35.0 90-91 27.43116327830932 33.0 26.5 35.0 2.0 35.0 92-93 26.95351094074097 33.0 25.5 35.0 2.0 35.0 94-95 26.473062818794737 33.0 25.0 35.0 2.0 35.0 96-97 25.905158890931943 32.5 24.0 34.5 2.0 35.0 98-99 25.038503391820157 32.0 19.0 34.0 2.0 35.0 100-101 22.51943976965403 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27368802738297404 1101 2 0.30095136356144536 1101 3 0.2463508490804216 1101 4 0.18958956638891067 1101 5 0.1439106253000304 1101 6 0.20797254226238948 1101 7 0.21491789629244096 1101 8 0.14489936797077263 1101 9 0.20228814742466739 1101 10-11 0.32609930858606617 1101 12-13 0.28251778661454807 1101 14-15 0.41674320257919106 1101 16-17 0.22245185908490583 1101 18-19 0.2647184068932589 1101 20-21 0.4194078185624903 1101 22-23 0.3472967731200498 1101 24-25 0.3506865124744607 1101 26-27 0.19593508661005643 1101 28-29 0.3442297008168538 1101 30-31 0.31716407047738215 1101 32-33 0.35107488294200806 1101 34-35 0.2842502950937984 1101 36-37 0.31240339704917375 1101 38-39 0.1560841551248089 1101 40-41 0.038117743308077934 1101 42-43 0.10855871677106421 1101 44-45 0.23093562358358355 1101 46-47 0.057079490886842166 1101 48-49 0.20710782823942964 1101 50-51 0.1382763146457009 1101 52-53 -0.007998048027609173 1101 54-55 0.06093319600083902 1101 56-57 0.201078505034765 1101 58-59 0.15795031354841882 1101 60-61 0.045246563937340056 1101 62-63 0.08276307870207233 1101 64-65 0.23276172996344968 1101 66-67 -0.1041631833204022 1101 68-69 0.12220039387224446 1101 70-71 0.09252074842599711 1101 72-73 -0.05324540679101375 1101 74-75 0.18852605124969202 1101 76-77 -0.01883020069608321 1101 78-79 -8.629446215699943E-4 1101 80-81 -0.12140884705741684 1101 82-83 -0.1724792003129494 1101 84-85 -0.29038741264744417 1101 86-87 -0.39424856028324484 1101 88-89 -0.24491300480798728 1101 90-91 -0.10410515182118374 1101 92-93 -0.14588913106867452 1101 94-95 -0.26340363703111436 1101 96-97 0.13975991397846244 1101 98-99 0.06637078428845555 1101 100-101 0.14814503701994042 1102 1 0.2569673572600877 1102 2 0.30179141732488546 1102 3 0.22481187058579621 1102 4 0.15065787476064685 1102 5 0.18243549089142874 1102 6 0.16244762866791973 1102 7 0.22017663285157596 1102 8 0.17273994976800822 1102 9 0.2822888674707542 1102 10-11 0.32166982509912145 1102 12-13 0.3578820099248432 1102 14-15 0.4409619525791939 1102 16-17 0.4068244589312897 1102 18-19 0.3844404691052503 1102 20-21 0.387285362725315 1102 22-23 0.3992048952398619 1102 24-25 0.30308186577708085 1102 26-27 0.43984589690192166 1102 28-29 0.3734629717461928 1102 30-31 0.2952248663683221 1102 32-33 0.40593759415552455 1102 34-35 0.4365898448249794 1102 36-37 0.40130628683412084 1102 38-39 0.4348553964842523 1102 40-41 0.44978669012067485 1102 42-43 0.5944992169630794 1102 44-45 0.6458717794975684 1102 46-47 0.6537144755258524 1102 48-49 0.5922466771257646 1102 50-51 0.6596790844229616 1102 52-53 0.7192934986313801 1102 54-55 0.7938231009547607 1102 56-57 0.49686743552631896 1102 58-59 0.6099280401229166 1102 60-61 0.5516993769173695 1102 62-63 0.38510442854845905 1102 64-65 0.6485583409465576 1102 66-67 0.5659459036611665 1102 68-69 0.6700162540872974 1102 70-71 0.2741259711602524 1102 72-73 0.2784804236621383 1102 74-75 0.31803353972895465 1102 76-77 0.335169655294699 1102 78-79 0.44895284358887366 1102 80-81 0.6224443595478064 1102 82-83 0.5508178907008769 1102 84-85 0.5827512682281295 1102 86-87 0.6142851858765113 1102 88-89 0.7358653650076796 1102 90-91 0.6681988756365733 1102 92-93 0.5447334687777143 1102 94-95 0.6254698508721113 1102 96-97 0.9134194281873711 1102 98-99 0.6840485156632639 1102 100-101 1.034020133026086 1103 1 0.17339825237049666 1103 2 0.19902130691479414 1103 3 0.29469554566465916 1103 4 0.12770588343573053 1103 5 0.11640138205861916 1103 6 0.14318747488243133 1103 7 0.20333416439731877 1103 8 0.2356515231118621 1103 9 0.26257031463163827 1103 10-11 0.2534447659508601 1103 12-13 0.261577544230839 1103 14-15 0.23062332876215663 1103 16-17 0.3009539936316017 1103 18-19 0.19217410475193475 1103 20-21 0.1615583319682159 1103 22-23 0.2811597453975878 1103 24-25 0.20823762287486858 1103 26-27 0.2115286650407171 1103 28-29 0.32911039879666504 1103 30-31 0.45751415067431367 1103 32-33 0.34123925030694124 1103 34-35 0.4431195668281305 1103 36-37 0.4949404548151932 1103 38-39 0.4091714946703746 1103 40-41 0.5449944484014324 1103 42-43 0.4696187457485621 1103 44-45 0.537073475081165 1103 46-47 0.5259837972924188 1103 48-49 0.4755779566998939 1103 50-51 0.4130271226721689 1103 52-53 0.354588993505196 1103 54-55 0.5668970855762083 1103 56-57 0.44963508142537734 1103 58-59 0.5940368729304879 1103 60-61 0.5040874881161059 1103 62-63 0.6514071690847345 1103 64-65 0.5311367123976609 1103 66-67 0.5475125933299338 1103 68-69 0.45267595440276054 1103 70-71 0.6228641415072538 1103 72-73 0.5388720344665536 1103 74-75 0.5907760476153925 1103 76-77 0.5848073721716709 1103 78-79 0.6525276168538561 1103 80-81 0.7845451087591613 1103 82-83 0.6190281627828931 1103 84-85 0.7276078336855427 1103 86-87 0.9827369800405492 1103 88-89 0.8861980739982123 1103 90-91 0.8027155850214278 1103 92-93 0.759505747957526 1103 94-95 0.9180479758721098 1103 96-97 1.030126348534374 1103 98-99 1.1642104340386616 1103 100-101 1.0673551093667832 1104 1 0.19983392742357609 1104 2 0.25517186691616445 1104 3 0.25710353725246193 1104 4 0.15175843674975198 1104 5 0.1668516466589125 1104 6 0.1537800821193258 1104 7 0.1533609539233325 1104 8 0.1985623555354934 1104 9 0.24689987450980766 1104 10-11 0.3089406300491646 1104 12-13 0.32242809019278695 1104 14-15 0.47441804363277384 1104 16-17 0.49207792169242026 1104 18-19 0.4312856666529328 1104 20-21 0.2489856635881651 1104 22-23 0.27341442067128696 1104 24-25 0.3369913798551849 1104 26-27 0.362369455030894 1104 28-29 0.3161823036035969 1104 30-31 0.42322391552817606 1104 32-33 0.44970746245706295 1104 34-35 0.4534732888531323 1104 36-37 0.3300706203945154 1104 38-39 0.16990485436345892 1104 40-41 0.22442302924419977 1104 42-43 0.2806655572219299 1104 44-45 0.4031251287255415 1104 46-47 0.35778747734239147 1104 48-49 0.3230570909541015 1104 50-51 0.35006743875992896 1104 52-53 0.2883056892535407 1104 54-55 0.4447955833571271 1104 56-57 0.5350961764436661 1104 58-59 0.5803072408495282 1104 60-61 0.369587234546799 1104 62-63 0.49566797873011126 1104 64-65 0.5990259556150406 1104 66-67 0.6179557952370018 1104 68-69 0.6304187291100085 1104 70-71 0.8577090774272804 1104 72-73 0.8544798701880225 1104 74-75 0.6754671228352223 1104 76-77 0.6355989525244929 1104 78-79 0.853075870950363 1104 80-81 1.0071954381127455 1104 82-83 1.057843464043291 1104 84-85 0.8051918052399394 1104 86-87 0.7442231683924021 1104 88-89 0.8364039378959625 1104 90-91 0.7644391543604598 1104 92-93 0.6151084120111392 1104 94-95 0.775563870513345 1104 96-97 0.9810248721019939 1104 98-99 1.0518437728158538 1104 100-101 1.4899394677218183 1105 1 0.1274751697600891 1105 2 0.15237735482488546 1105 3 0.045514995585797635 1105 4 0.09284537476064969 1105 5 0.03438861589143016 1105 6 0.12240856616791973 1105 7 0.0027938203515773807 1105 8 0.11688057476801106 1105 9 0.1432263674707528 1105 10-11 0.1779198250991243 1105 12-13 0.10387810367484462 1105 14-15 0.13256351507919106 1105 16-17 0.047254146431292554 1105 18-19 0.24225296910524463 1105 20-21 0.3522267689753136 1105 22-23 0.10750567648986475 1105 24-25 0.16284749077707517 1105 26-27 0.10703339690191882 1105 28-29 0.07209578424619423 1105 30-31 0.015635022618319283 1105 32-33 8.594691555217082E-4 1105 34-35 -0.029914061425017735 1105 36-37 -0.044299181915882 1105 38-39 0.06073430273425373 1105 40-41 0.11541169012067343 1105 42-43 -0.013508595536926293 1105 44-45 -0.16018290800243307 1105 46-47 -0.4416956807241448 1105 48-49 -0.6394916041242382 1105 50-51 -0.5494029468270369 1105 52-53 -0.45355806386861985 1105 54-55 -0.3394776802952393 1105 56-57 -0.551472408223681 1105 58-59 -0.5181969598770806 1105 60-61 -0.4112889043326291 1105 62-63 -0.33071588395154095 1105 64-65 -0.5062268153034424 1105 66-67 -0.39870253383883636 1105 68-69 -0.4405306209126998 1105 70-71 -0.38642090383974903 1105 72-73 -0.2375352013378631 1105 74-75 -0.43079458527104464 1105 76-77 -0.3365100322053003 1105 78-79 -0.19850809391112634 1105 80-81 -0.321793921702195 1105 82-83 -0.5211547655491238 1105 84-85 -0.6288698255218712 1105 86-87 -0.6472382516234916 1105 88-89 -0.6405018224923218 1105 90-91 -0.4908831556134281 1105 92-93 -0.34110637497228424 1105 94-95 -0.17619030537789016 1105 96-97 -0.1559165093126289 1105 98-99 -0.28157648433673543 1105 100-101 -0.3840462732239125 1106 1 -0.06256389273990948 1106 2 0.10632770764746624 1106 3 0.030221816822354697 1106 4 0.007101087126244465 1106 5 0.013803098418314619 1106 6 0.07341033028082222 1106 7 0.12782112209888652 1106 8 0.0584170331013425 1106 9 0.0916890690836567 1106 10-11 0.06626827940020519 1106 12-13 -0.029924609698809945 1106 14-15 0.1678184713963944 1106 16-17 0.3362767438775265 1106 18-19 0.0796185605030928 1106 20-21 0.038301827779079645 1106 22-23 0.10812626620760568 1106 24-25 0.2045267582501893 1106 26-27 0.21052802055783815 1106 28-29 0.16327731986446992 1106 30-31 0.095581889217776 1106 32-33 0.2466970027576778 1106 34-35 0.11919653165293909 1106 36-37 -0.002112731983082483 1106 38-39 0.13158338707565065 1106 40-41 0.1454142102819631 1106 42-43 -0.15280841072509332 1106 44-45 -0.03841921512609048 1106 46-47 0.10569700240757385 1106 48-49 -0.07975853120757392 1106 50-51 0.014774010498236123 1106 52-53 -0.09117777152990669 1106 54-55 0.041962381868735577 1106 56-57 -0.18133316931238852 1106 58-59 -0.21332464804912377 1106 60-61 -0.06274114727617786 1106 62-63 -0.053949670913908676 1106 64-65 -0.13044871550506087 1106 66-67 0.04437752328482247 1106 68-69 -0.15038025128904664 1106 70-71 -0.1116267170117915 1106 72-73 0.05005720457611318 1106 74-75 0.0845321956429359 1106 76-77 0.11135413110114811 1106 78-79 0.16557330729855124 1106 80-81 0.0018630423435048726 1106 82-83 0.05885720521700577 1106 84-85 0.12382149672275133 1106 86-87 0.19008323695177864 1106 88-89 -0.008430669938558566 1106 90-91 0.1483526054752815 1106 92-93 0.3883658612508327 1106 94-95 0.4285318468398529 1106 96-97 0.5894526943164031 1106 98-99 1.0410251621686406 1106 100-101 1.1269468803379148 1107 1 0.20958800599426297 1107 2 0.26369806289450537 1107 3 0.22081661742124226 1107 4 0.09279592855811813 1107 5 0.10603121873953114 1107 6 0.25519881537677946 1107 7 0.2656869176617036 1107 8 0.14913433267940235 1107 9 0.17094590860999404 1107 10-11 0.30567892320038936 1107 12-13 0.30177540391218827 1107 14-15 0.3338318101741251 1107 16-17 0.38473189564015087 1107 18-19 0.3286453741685378 1107 20-21 0.49744161272531073 1107 22-23 0.3878322686575899 1107 24-25 0.1863492708403669 1107 26-27 0.26874225766140825 1107 28-29 0.4541443404170806 1107 30-31 0.35214239010249315 1107 32-33 0.3837461384593226 1107 34-35 0.42362010590093035 1107 36-37 0.436304308986017 1107 38-39 0.4194627936361499 1107 40-41 0.4222006537282681 1107 42-43 0.5769112027225631 1107 44-45 0.5704959883583243 1107 46-47 0.18191858944990003 1107 48-49 0.28205581478399466 1107 50-51 -0.046101176652989295 1107 52-53 0.13330160780859757 1107 54-55 0.007465308233243206 1107 56-57 0.055579856412400375 1107 58-59 0.14299766037608208 1107 60-61 0.31233031046167525 1107 62-63 0.2114988114598475 1107 64-65 0.1974606352503585 1107 66-67 0.17470282612951848 1107 68-69 0.1356412540873002 1107 70-71 0.22191078128683372 1107 72-73 0.4453069663836544 1107 74-75 0.322187020741616 1107 76-77 0.19655217111748513 1107 78-79 0.4968513799812797 1107 80-81 0.4113090747376802 1107 82-83 0.26963711538442325 1107 84-85 0.30461637898762106 1107 86-87 0.1741447586613205 1107 88-89 0.30640531753932265 1107 90-91 0.30194590886442185 1107 92-93 0.6315807789043006 1107 94-95 0.8487441784037557 1107 96-97 0.8915147604658493 1107 98-99 0.9602302799037723 1107 100-101 1.1035019368235588 1108 1 0.23275630430442362 1108 2 0.19688070303917016 1108 3 0.17294918585673713 1108 4 0.08412847205129026 1108 5 0.05248629138404226 1108 6 0.07198811512112258 1108 7 0.14764738408310762 1108 8 0.13468961109806088 1108 9 0.1873111888993222 1108 10-11 0.19129343963114565 1108 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3 5.0 4 14.0 5 36.0 6 71.0 7 179.0 8 380.0 9 742.0 10 1364.0 11 2200.0 12 3440.0 13 4996.0 14 6453.0 15 9367.0 16 13080.0 17 18911.0 18 16583.0 19 9399.0 20 7876.0 21 7830.0 22 7961.0 23 8394.0 24 9390.0 25 10858.0 26 12816.0 27 15301.0 28 19119.0 29 23330.0 30 28538.0 31 35586.0 32 45517.0 33 59565.0 34 81719.0 35 119405.0 36 172200.0 37 235781.0 38 155311.0 39 11411.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.40984855700543 19.932547688524448 16.009923022971883 20.647680731498237 2 15.763220134149169 21.74825041640263 39.33991959360489 23.148609855843315 3 19.109590938524775 26.10056686162274 30.835610129405122 23.95423207044736 4 12.910615023283153 17.655461260969574 36.61805152160758 32.81587219413969 5 12.821436457390757 38.543280325235635 33.62058440237086 15.01469881500275 6 30.588019377871966 37.43165283274985 16.53352171007296 15.446806079305222 7 27.0795026023 34.952109369318826 20.322352770073028 17.64603525830814 8 26.81537694393368 34.10008553135053 19.647884397627287 19.436653127088505 9 27.13923603536219 16.434398839266855 20.547781552238188 35.878583573132765 10-11 24.72497719956559 27.569068976129557 26.15451141276989 21.551442411534964 12-13 26.28275496670395 25.348976531739464 27.552956820755405 20.815311680801184 14-15 23.186570885405306 26.045335078588423 27.154948525363338 23.613145510642937 16-17 20.793164763853827 29.26912248989726 28.06008317664128 21.877629569607628 18-19 20.594096605409597 26.748285044479765 32.3936138900143 20.264004460096334 20-21 21.37387761744978 25.57833217329275 33.09699670686987 19.950793502387608 22-23 21.566701670214634 25.38045797799538 32.86724940504068 20.1855909467493 24-25 21.28696114383464 25.668798024814482 32.78893667563534 20.255304155715535 26-27 20.44662621512613 26.2263803027719 32.89807094804294 20.428922534059026 28-29 20.570013249780217 26.016707449094827 33.138778137386495 20.274501163738456 30-31 20.57089579372747 26.058883313768472 32.772012202934384 20.598208689569677 32-33 20.418748679804562 26.21882174504732 32.59138349556587 20.77104607958225 34-35 21.135420021261638 26.062475976771488 32.54502515729804 20.257078844668836 36-37 21.34188993119401 25.845054937444377 32.210474647053324 20.602580484308287 38-39 20.798957693073508 26.2943564793573 32.28939599871875 20.61728982885043 40-41 21.05206886879677 26.085164882731206 32.38634986373757 20.476416384734453 42-43 20.843549995844068 26.37076441415233 31.82265259191533 20.96303299808827 44-45 20.848751852061863 26.730593010406174 31.537534904034736 20.883120233497227 46-47 21.4568118621941 27.079372747010733 30.621998178563143 20.841817212232023 48-49 21.26380361724894 27.185810799112136 30.245028273824982 21.305357309813942 50-51 21.716219445050434 27.611822718096285 29.819838771672845 20.852119065180432 52-53 21.813264631228293 27.604507536800988 29.645529688381934 20.936698143588785 54-55 21.631337370967127 27.908715194453965 29.543333575729875 20.916613858849033 56-57 21.54991810459757 28.057053219891753 29.311888165162074 21.0811405103486 58-59 21.346564721607574 27.468072912879222 29.500351474982946 21.68501089053026 60-61 21.362753347669358 27.885038246711197 29.682408590533377 21.069799815086068 62-63 21.381625649709296 27.937110217706724 29.93722795316899 20.744036179414994 64-65 21.441921789024978 27.772843276785686 29.754867841943604 21.030367092245736 66-67 21.247701561379998 27.900750736712343 29.599301205403368 21.252246496504295 68-69 21.4851761145292 28.00791080476726 29.54644026771036 20.96047281299318 70-71 21.592164358705325 27.972820422254774 29.042438439935868 21.392576779104033 72-73 21.53792813288871 28.041946721240517 28.66815221117587 21.751972934694912 74-75 21.402498330276686 28.658901605574776 28.49982880736955 21.438771256778992 76-77 21.658000990363004 27.809852034226388 29.107669080243642 21.42447789516696 78-79 21.529747249664975 27.783145129734095 29.11030947112538 21.576798149475557 80-81 21.634107617138127 28.30789035365655 28.635255537895237 21.422746491310086 82-83 21.75388718895238 28.159346070087786 28.600083888223487 21.486682852736344 84-85 21.39600234433097 28.138421143302857 28.50582663204998 21.95974988031619 86-87 21.440969516903696 28.50925262221114 28.301743869964646 21.74803399092052 88-89 21.574374184075932 28.14743250122064 28.33931533365884 21.93887798104459 90-91 21.28511361506331 28.02639091927965 28.486210394032653 22.202285071624395 92-93 21.301106648809768 27.845597255323955 28.62755809192033 22.225738003945946 94-95 21.281723647167595 27.565247954346344 28.750610319859547 22.402418078626514 96-97 21.358814403894964 27.484780960098064 28.71546282156498 22.440941814441988 98-99 21.079512031058393 27.57709300679716 28.39536686622841 22.948028095916033 100-101 20.90899172840992 27.75645349406061 28.72647991154343 22.60807486598604 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2306.0 1 1577.5 2 957.5 3 1121.0 4 946.0 5 976.0 6 1887.0 7 3697.5 8 5467.0 9 6027.0 10 5031.0 11 3905.5 12 3743.0 13 3759.0 14 3840.0 15 4004.0 16 4035.0 17 4058.5 18 4176.5 19 4252.0 20 4450.5 21 4766.5 22 5120.0 23 5606.0 24 6343.0 25 7231.5 26 8425.5 27 9998.5 28 11153.0 29 12613.0 30 14703.5 31 16790.5 32 19470.0 33 21504.5 34 22476.5 35 24314.0 36 28441.5 37 31828.5 38 32530.5 39 32320.5 40 32057.5 41 32100.5 42 31277.0 43 33168.5 44 36225.0 45 36735.5 46 36661.5 47 38263.5 48 42311.5 49 41463.0 50 38443.5 51 37086.5 52 34761.0 53 34529.5 54 35760.5 55 34567.5 56 31151.5 57 28088.0 58 26254.5 59 24271.5 60 19960.0 61 16263.0 62 13754.0 63 9857.0 64 6626.5 65 4920.0 66 3621.5 67 2823.5 68 2176.5 69 1587.5 70 1100.5 71 699.5 72 519.5 73 411.5 74 296.0 75 225.0 76 161.5 77 94.0 78 53.5 79 31.0 80 28.0 81 18.5 82 8.0 83 5.5 84 4.5 85 2.5 86 1.5 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020949986668190302 2 0.0 3 0.0 4 4.3285096421880786E-4 5 0.023979943417721958 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.328509642188079E-5 12-13 0.003462807713750463 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.522721212813774E-4 24-25 0.0 26-27 4.3285096421880786E-4 28-29 0.0016015485676095893 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.7314038568752316E-4 40-41 6.925615427500927E-4 42-43 0.013504950083626807 44-45 4.328509642188079E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.597105785312847E-4 70-71 0.0 72-73 0.0 74-75 4.328509642188079E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.002034399531828397 84-85 4.3285096421880786E-4 86-87 0.0 88-89 0.0 90-91 0.00982571688776694 92-93 0.0019911144354065164 94-95 0.0 96-97 0.0 98-99 0.0015149783747658277 100-101 0.01861259146140874 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1155132.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.829065606357446 #Duplication Level Percentage of deduplicated Percentage of total 1 67.20595817728604 26.767505173812395 2 14.815941094889054 11.80210179776527 3 6.445419850668857 7.701451502784256 4 3.3848073386050093 5.3925485421671615 5 2.130377180034899 4.242546623494838 6 1.293080395267928 3.0901310338452546 7 0.8633974702925743 2.4071820142052194 8 0.6499150297117166 2.070840668555656 9 0.4844139413616731 1.7364379186015437 >10 2.464031418885201 17.610227392647623 >50 0.16515953244944048 4.594236722030589 >100 0.08937369008117331 6.9329611534477396 >500 0.0052701927349596365 1.362820237901071 >1k 0.002195913639566515 1.4675112804352501 >5k 2.195913639566515E-4 0.696890749300201 >10k+ 4.39182727913303E-4 2.124607189006085 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13457 1.1649750850984997 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10835 0.9379880394621567 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7968 0.6897912965790923 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2961 0.25633434101037805 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2476 0.21434779748115365 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2326 0.20136226855458944 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2068 0.17902715880089895 No Hit TCCCAGGACCGAGTGGGAGTAGGGGATTACTGAGTGGCATCCCCAAGCTG 1346 0.11652347956770308 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1214 0.10509621411232656 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.7314038568752316E-4 2 0.0 0.0 0.0 0.0 1.7314038568752316E-4 3 0.0 0.0 0.0 0.0 1.7314038568752316E-4 4 0.0 0.0 0.0 0.0 1.7314038568752316E-4 5 0.0 0.0 0.0 0.0 1.7314038568752316E-4 6 0.0 0.0 0.0 0.0 1.7314038568752316E-4 7 0.0 0.0 0.0 0.0 1.7314038568752316E-4 8 0.0 0.0 0.0 0.0 2.5971057853128475E-4 9 0.0 0.0 0.0 0.0 3.4628077137504633E-4 10-11 0.0 0.0 0.0 0.0 3.4628077137504633E-4 12-13 0.0 0.0 0.0 4.328509642188079E-5 3.4628077137504633E-4 14-15 0.0 0.0 0.0 8.657019284376158E-5 3.4628077137504633E-4 16-17 0.0 0.0 0.0 3.0299567495316554E-4 3.4628077137504633E-4 18-19 0.0 0.0 0.0 4.328509642188079E-4 4.328509642188079E-4 20-21 0.0 0.0 0.0 6.925615427500927E-4 4.328509642188079E-4 22-23 0.0 0.0 0.0 0.001255267796234543 4.761360606406887E-4 24-25 0.0 0.0 0.0 0.002250825013937801 6.925615427500927E-4 26-27 0.0 0.0 0.0 0.0030299567495316554 7.791317355938542E-4 28-29 0.0 0.0 0.0 0.005367351956313218 8.22416832015735E-4 30-31 0.0 0.0 0.0 0.010648133719782675 8.657019284376158E-4 32-33 0.0 0.0 0.0 0.01921858281131507 9.089870248594966E-4 34-35 0.0 0.0 0.0 0.026360623720925402 0.001038842314125139 36-37 0.0 0.0 0.0 0.0331130987627388 0.0011254125069689006 38-39 0.0 0.0 0.0 0.040211854575927256 0.0012119826998126621 40-41 0.0 0.0 0.0 0.05124955416350685 0.0012119826998126621 42-43 0.0 0.0 0.0 0.06592320185052444 0.0012119826998126621 44-45 0.0 0.0 0.0 0.08059684953754204 0.0012119826998126621 46-47 0.0 0.0 0.0 0.09955572177032582 0.0012119826998126621 48-49 0.0 0.0 0.0 0.11951015122081285 0.0012119826998126621 50-51 0.0 0.0 0.0 0.14136912491386266 0.0012119826998126621 52-53 0.0 0.0 0.0 0.18885287568866588 0.001255267796234543 54-55 0.0 0.0 0.0 0.25243868233240874 0.0013418379890783045 56-57 0.0 0.0 0.0 0.3162841995546829 0.0013851230855001853 58-59 0.0 0.0 0.0 0.41181440735777386 0.0013851230855001853 60-61 0.0 0.0 0.0 0.5318439797356492 0.0013851230855001853 62-63 0.0 0.0 0.0 0.620188861532708 0.001471693278343947 64-65 0.0 0.0 0.0 0.6936436701606397 0.001471693278343947 66-67 0.0 0.0 0.0 0.8574777601174584 0.0015582634711877085 68-69 0.0 0.0 0.0 1.023605960184637 0.00164483366403147 70-71 0.0 0.0 0.0 1.1130762544886645 0.0016881187604533509 72-73 0.0 0.0 0.0 1.2946572339784543 0.0019045442425627548 74-75 0.0 0.0 0.0 1.5381359013515339 0.0019045442425627548 76-77 0.0 0.0 0.0 1.7793637437106755 0.0019045442425627548 78-79 0.0 0.0 0.0 2.0440521083304763 0.0019045442425627548 80-81 0.0 0.0 0.0 2.334971241381937 0.0019045442425627548 82-83 0.0 0.0 0.0 2.586327796303799 0.0019045442425627548 84-85 0.0 0.0 0.0 2.8577253508689915 0.0019045442425627548 86-87 0.0 0.0 0.0 3.2586319139284514 0.0019045442425627548 88-89 0.0 0.0 0.0 3.7692229113209574 0.0019045442425627548 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6650 0.0 40.65564 1 GTATCAA 10480 0.0 34.185368 1 GTAAGGT 395 0.0 28.864836 4 TAAGGTG 455 0.0 28.190796 5 TATCAAC 13200 0.0 27.280367 2 ATCAACG 13385 0.0 26.938807 3 TCAACGC 13450 0.0 26.84394 4 CAACGCA 13600 0.0 26.547867 5 AACGCAG 13945 0.0 25.92065 6 GGTAAGG 415 0.0 25.18428 3 GAGTACT 9435 0.0 24.166662 12-13 ACGCAGA 15325 0.0 23.526571 7 CGCAGAG 15435 0.0 23.112698 8 GTACTTT 9965 0.0 22.641024 14-15 GTACATG 6540 0.0 22.449718 1 AGTACTT 9845 0.0 22.09872 12-13 GCAGAGT 15875 0.0 22.083096 9 GTACTAA 130 2.9352724E-4 21.929972 1 TACATGG 6670 0.0 21.08244 2 GGTGTGC 595 0.0 20.757437 8 >>END_MODULE