FastQCFastQC Report
Thu 26 May 2016
SRR937813_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937813_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091397
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104640.9587711895854578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78890.7228350453592964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55820.5114545852700713No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23680.21696962700099046No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16910.15493903684910257No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16750.15347302585585262No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14390.13184936370541608No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13140.12039615282065097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56550.037.2640571
GTATCAA89150.035.0560531
TAAGGTA2000.030.8925114
ACTAACG650.00582646229.2486923
TATCAAC110900.027.9937522
ATCAACG110700.027.9507313
TCAACGC110600.027.9317474
CAACGCA111450.027.6760715
AACGCAG114850.026.8833226
GTGTAAG3650.026.0649051
TGTACGC1151.2739837E-424.804642
GAGTACT69050.024.41829712-13
GTACATG65400.024.3661061
ACGCAGA126300.024.2968317
CGCAGAG126300.024.1087748
GTACGCT1201.7036407E-423.764563
TACATGG65300.023.5890982
TAAGGTG3900.023.154155
GTACTTT72150.023.10529514-15
ACATGGG65250.022.8722523
Produced by FastQC (version 0.11.5)