FastQCFastQC Report
Thu 26 May 2016
SRR937813_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937813_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091397
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127691.169968398300527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102430.9385219127411932No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76880.7044182822565941No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28880.2646149842816134No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23300.21348785089202188No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22700.20799030966733462No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19740.1808691062922108No Hit
TCCCAGGACCGAGTGGGAGTAGGGGATTACTGAGTGGCATCCCCAAGCTG12160.11141683548699512No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11640.10665229975893281No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11290.10344540071119859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT5100.040.9860044
GGTAAGG5250.039.8149763
GGTATCA62750.038.475321
GTATCAA99650.035.769771
TAAGGTG6350.035.1622965
AGGTAAG5600.033.933222
TATCAAC125550.028.3411982
TCAACGC127000.028.0550234
ATCAACG127000.028.0176163
CAACGCA128650.027.6952045
AACGCAG131650.027.0591346
GGTGTGC8300.026.3252898
GTGTGCA8550.024.4444319
ACGCAGA144550.024.3825557
CGCAGAG144900.024.2908768
GTACATG63250.024.195111
GAGTACT88950.024.08374412-13
CCGATAA1401.7793815E-523.7499899
TACATGG63500.023.4914052
GCAGAGT150200.023.1807469