FastQCFastQC Report
Thu 26 May 2016
SRR937812_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937812_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031288
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107211.0395738144921691No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78350.759729580873626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56190.5448526502780988No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21740.21080435339109926No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17490.16959375072724592No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16450.15950927384009123No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14760.14312199889846483No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13760.13342538650696995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45650.042.927141
GTATCAA78100.038.854841
TATCAAC98600.030.662112
ATCAACG99900.030.261633
TCAACGC100200.030.1250514
CAACGCA102150.029.549985
AACGCAG104650.028.9634136
GACGCGT350.00840795127.1330188-89
CGCAGAG116400.025.9580738
ACGCAGA117200.025.8619567
GCAGAGT119800.024.7058339
GAGTACT67800.024.30815312-13
GTACATG61650.023.5311011
TAAGGTG4850.023.5229245
TACATGG60400.023.3744472
GTACTTT69550.023.3522314-15
TAAGGTA2653.092282E-1123.3195024
GTATAGG3100.023.0146851
GTAAGGT4550.022.9843964
AGTACTT70400.022.97188612-13
Produced by FastQC (version 0.11.5)