FastQCFastQC Report
Thu 26 May 2016
SRR937812_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937812_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031288
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117101.1354733110440536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95810.929032433229127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70630.6848717332112852No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28850.2797472674946281No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21230.20585908107143688No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20370.19751999441475127No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17990.1744420569229934No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC11130.10792329591733832No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10770.10443251545640016No Hit
TCCCAGGACCGAGTGGGAGTAGGGGATTACTGAGTGGCATCCCCAAGCTG10640.10317195584550581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61900.036.7029081
GTATCAA94100.033.0837631
GTAAGGT4800.029.6900924
GGTAAGG4850.029.3840073
AGGTAAG4100.027.8073042
TAAGGTG6350.027.6795835
CCTATCG700.00838760427.1452273
TATCAAC120150.025.8574392
ATCAACG120350.025.7355253
TCAACGC121250.025.5444984
CAACGCA123000.025.2583035
AACGCAG125500.024.7137016
GTACATG64100.023.7276741
GGTGTGC7100.023.418678
GAGTACT81000.023.22537612-13
TACATGG65200.022.8777642
ACATGGG62250.022.5883183
ACGCAGA137550.022.4494327
CGCAGAG138600.022.2108088
AGTACTT83850.022.20933512-13
Produced by FastQC (version 0.11.5)