##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937812_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1031288 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18923326946498 33.0 31.0 34.0 31.0 34.0 2 32.38313448813523 34.0 31.0 34.0 31.0 34.0 3 32.48176358107531 34.0 31.0 34.0 31.0 34.0 4 36.132734987704694 37.0 35.0 37.0 35.0 37.0 5 35.98567228553033 37.0 35.0 37.0 35.0 37.0 6 36.095926647066584 37.0 35.0 37.0 35.0 37.0 7 36.05194572224248 37.0 35.0 37.0 35.0 37.0 8 36.05309380114963 37.0 35.0 37.0 35.0 37.0 9 37.689956636749386 39.0 37.0 39.0 35.0 39.0 10-11 37.65179707317452 39.0 37.0 39.0 35.0 39.0 12-13 37.57147130578461 39.0 37.0 39.0 35.0 39.0 14-15 38.72719017384087 40.0 38.0 41.0 34.5 41.0 16-17 38.7183240763007 40.0 38.0 41.0 34.5 41.0 18-19 38.7692026863495 40.0 38.0 41.0 35.0 41.0 20-21 38.70918695844419 40.0 38.0 41.0 34.5 41.0 22-23 38.55901212852278 40.0 38.0 41.0 34.0 41.0 24-25 38.493033468827335 40.0 38.0 41.0 34.0 41.0 26-27 38.361364138824456 40.0 38.0 41.0 34.0 41.0 28-29 38.217172603579215 40.0 38.0 41.0 34.0 41.0 30-31 38.171805548013744 40.0 37.5 41.0 34.0 41.0 32-33 37.99209435191722 40.0 37.0 41.0 33.5 41.0 34-35 37.81688820193777 40.0 36.5 41.0 33.0 41.0 36-37 37.60788402463715 40.0 36.0 41.0 33.0 41.0 38-39 37.42247461426876 40.0 36.0 41.0 33.0 41.0 40-41 37.18069540225427 40.0 36.0 41.0 33.0 41.0 42-43 36.90499307661875 39.5 35.0 41.0 31.5 41.0 44-45 36.484229914437094 39.0 35.0 41.0 31.0 41.0 46-47 35.944070909387094 39.0 35.0 41.0 29.5 41.0 48-49 35.458607585853805 39.0 35.0 41.0 27.5 41.0 50-51 35.127541482107816 39.0 35.0 40.0 26.0 41.0 52-53 34.86073531351087 38.5 35.0 40.0 25.5 41.0 54-55 34.62182242011931 38.0 34.5 40.0 24.5 41.0 56-57 34.29693645228103 38.0 34.0 40.0 23.5 41.0 58-59 34.00702616533887 38.0 34.0 40.0 23.0 41.0 60-61 33.636299462419814 37.0 33.5 40.0 22.0 41.0 62-63 33.22570174383877 36.5 33.0 39.5 20.5 41.0 64-65 32.77932158621064 36.0 33.0 39.0 18.5 40.5 66-67 32.33832983608846 35.5 32.0 39.0 16.0 40.0 68-69 31.880507675838366 35.0 32.0 38.0 12.0 40.0 70-71 31.457537079845785 35.0 31.0 37.5 7.5 40.0 72-73 31.021564296297445 35.0 31.0 37.0 6.5 39.0 74-75 30.543350645018656 34.5 30.5 36.5 2.0 39.0 76-77 29.351957939974092 33.5 29.0 35.0 2.0 37.5 78-79 29.883190728487094 34.0 30.0 35.0 2.0 37.5 80-81 29.692896649626487 34.0 30.0 35.0 2.0 37.0 82-83 29.35256688723228 34.0 30.0 35.0 2.0 37.0 84-85 28.96335698660316 34.0 29.0 35.0 2.0 36.0 86-87 28.56467591982065 34.0 29.0 35.0 2.0 36.0 88-89 28.128216366330257 34.0 28.5 35.0 2.0 35.0 90-91 27.62537283474645 33.0 27.0 35.0 2.0 35.0 92-93 27.212825612244103 33.0 26.5 35.0 2.0 35.0 94-95 26.75931456586327 33.0 25.5 35.0 2.0 35.0 96-97 26.215615327629138 33.0 25.0 35.0 2.0 35.0 98-99 25.42962247209315 32.5 22.0 34.0 2.0 35.0 100-101 23.12236736973571 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2533845456818469 1101 2 0.20778078158196678 1101 3 0.1785881901296591 1101 4 0.10562948292176344 1101 5 0.07054001941862253 1101 6 0.10692266472836565 1101 7 0.11635006986362839 1101 8 0.01828698117629557 1101 9 0.2652469520176268 1101 10-11 0.19484428068610526 1101 12-13 0.3037489670608551 1101 14-15 0.31410602889545913 1101 16-17 0.14225106812931898 1101 18-19 0.20764246288194244 1101 20-21 0.32340871580370845 1101 22-23 0.23030912310079543 1101 24-25 0.1684406809519814 1101 26-27 0.2020165118445405 1101 28-29 0.18332938047725378 1101 30-31 0.37996833776200134 1101 32-33 0.30981232581123663 1101 34-35 0.11391081528087454 1101 36-37 0.4024263621899351 1101 38-39 0.38394420853264677 1101 40-41 0.2205932612916186 1101 42-43 0.20698054314733838 1101 44-45 0.22482952436185144 1101 46-47 0.12428085229581853 1101 48-49 0.08310723607426951 1101 50-51 0.1343286310962526 1101 52-53 0.15117396819037765 1101 54-55 -0.008439194119432614 1101 56-57 0.051128752859277427 1101 58-59 0.2185995885059242 1101 60-61 0.22449317864466423 1101 62-63 0.2670675369214024 1101 64-65 0.22669602535678735 1101 66-67 0.3604845592722228 1101 68-69 0.1184831415373715 1101 70-71 0.33261049869884474 1101 72-73 0.226698184840064 1101 74-75 0.22467843492134065 1101 76-77 0.10367326690116485 1101 78-79 -0.010131596174925761 1101 80-81 0.14459218901386706 1101 82-83 0.22031079112552732 1101 84-85 0.3610354722900375 1101 86-87 0.3381538485872788 1101 88-89 0.4565023669436812 1101 90-91 0.4451334798340625 1101 92-93 0.8040577742746429 1101 94-95 0.8956871299697866 1101 96-97 1.0689344417817992 1101 98-99 1.3198555931797742 1101 100-101 1.9588303309932051 1102 1 0.24661006841321864 1102 2 0.2614306828540194 1102 3 0.3038354090662736 1102 4 0.16677696188720148 1102 5 0.02446192138025083 1102 6 0.08962731571668314 1102 7 0.16441310251169483 1102 8 0.2602542612811334 1102 9 0.2051689593316297 1102 10-11 0.31151326191262996 1102 12-13 0.3112301832104123 1102 14-15 0.31198661037674924 1102 16-17 0.29072409712197356 1102 18-19 0.3238636964944632 1102 20-21 0.28003705655161326 1102 22-23 0.41290155784217575 1102 24-25 0.33316790608203206 1102 26-27 0.2137792646703005 1102 28-29 0.07858437314249755 1102 30-31 0.32615522011372633 1102 32-33 0.2946798930811241 1102 34-35 0.25118137455834955 1102 36-37 0.38234117042465954 1102 38-39 0.42112561734936094 1102 40-41 0.2669155853012697 1102 42-43 0.13496101783961478 1102 44-45 0.20013875754018784 1102 46-47 0.17950026458916568 1102 48-49 0.21288221296858012 1102 50-51 0.3283266581335056 1102 52-53 0.24491763308041925 1102 54-55 0.3024899979076281 1102 56-57 0.3783532444143276 1102 58-59 0.2911674250628806 1102 60-61 0.26412602560117904 1102 62-63 0.2940317602109843 1102 64-65 0.3185050374547558 1102 66-67 0.347560934958814 1102 68-69 0.14137094604166478 1102 70-71 0.10461557796054777 1102 72-73 0.32363354796257937 1102 74-75 0.3748283226252802 1102 76-77 0.16330191572496844 1102 78-79 0.3396550652560464 1102 80-81 0.3781137200074163 1102 82-83 0.231674880968761 1102 84-85 0.35945678284171834 1102 86-87 0.5436811771542125 1102 88-89 0.5841060472534814 1102 90-91 0.6773648432375197 1102 92-93 0.7801108591040595 1102 94-95 0.8234018293403906 1102 96-97 1.0467577658409333 1102 98-99 1.115531436677216 1102 100-101 1.7688843799565213 1103 1 0.22606593173003375 1103 2 0.22399066769242637 1103 3 0.26500170742066587 1103 4 0.16118523067649448 1103 5 -0.009006221396177239 1103 6 0.18658999102260054 1103 7 0.17098729776726174 1103 8 0.09105589472817854 1103 9 0.24548753156732772 1103 10-11 0.1451983692238059 1103 12-13 0.2125032742443338 1103 14-15 0.2980604730785146 1103 16-17 0.06805161612759747 1103 18-19 0.18113598653858531 1103 20-21 0.19197734705344516 1103 22-23 0.26449674332137363 1103 24-25 0.29264238168402557 1103 26-27 0.05824912742927779 1103 28-29 0.20276014034052992 1103 30-31 0.10155044063622398 1103 32-33 0.24641802305602312 1103 34-35 0.2585015809562563 1103 36-37 0.16780508297373586 1103 38-39 0.17076803261966234 1103 40-41 0.24649258044527045 1103 42-43 0.17717881197093277 1103 44-45 0.2416811635132845 1103 46-47 0.31977941310840663 1103 48-49 0.35127957184776903 1103 50-51 0.12550236454213604 1103 52-53 0.2177130338256248 1103 54-55 0.2965759934592782 1103 56-57 0.2444131791908717 1103 58-59 0.2941863341433901 1103 60-61 0.3034478304028241 1103 62-63 0.2604009163825438 1103 64-65 0.06348231294517603 1103 66-67 0.11789499061329423 1103 68-69 0.3411325264231344 1103 70-71 0.3805932146115545 1103 72-73 0.4118586403982327 1103 74-75 0.1761689971731144 1103 76-77 0.27636797235208377 1103 78-79 0.2507894491733147 1103 80-81 0.32054928323912435 1103 82-83 0.3684260091485818 1103 84-85 0.5514735249587623 1103 86-87 0.2913577703729757 1103 88-89 0.4331458428580497 1103 90-91 0.5267407684880077 1103 92-93 0.8640945496645394 1103 94-95 0.6666089710226117 1103 96-97 0.7542713361814215 1103 98-99 0.9893536419737607 1103 100-101 1.8325156886451204 1104 1 0.0863374441057232 1104 2 0.20310178228915277 1104 3 0.10443139026409654 1104 4 0.09680887782020164 1104 5 -0.07926847945057602 1104 6 -0.10403491094433548 1104 7 0.12773510966925983 1104 8 0.037921973719306834 1104 9 0.14894064823451458 1104 10-11 0.17502198813399872 1104 12-13 0.09583020275078269 1104 14-15 -0.03309129779076869 1104 16-17 -0.09663608974183546 1104 18-19 0.06295320585031305 1104 20-21 0.058177899175667847 1104 22-23 0.18115687445040152 1104 24-25 0.06777217746154918 1104 26-27 -0.0553888403845022 1104 28-29 -0.01553596657041112 1104 30-31 0.2226310787138388 1104 32-33 -0.07221492546485564 1104 34-35 0.08035785664091577 1104 36-37 0.05739436364632411 1104 38-39 -0.07442442589274378 1104 40-41 -0.001501663053183222 1104 42-43 0.2581992185336617 1104 44-45 0.18647678418022196 1104 46-47 0.13113830823655093 1104 48-49 -0.01922071048745977 1104 50-51 0.11191302112839452 1104 52-53 0.32500238037643214 1104 54-55 0.2767478258148941 1104 56-57 0.38998247680357423 1104 58-59 0.15689298784354833 1104 60-61 0.206893104424843 1104 62-63 0.3375999940874621 1104 64-65 0.14656925650950114 1104 66-67 0.10640159412051986 1104 68-69 0.10084368180162073 1104 70-71 0.2323358503677433 1104 72-73 0.2294779270312297 1104 74-75 0.03218391388422148 1104 76-77 -0.3441636805638133 1104 78-79 -0.004467083750107292 1104 80-81 0.19144169783271892 1104 82-83 0.16351337621574658 1104 84-85 0.2808316828782651 1104 86-87 0.16925562776231473 1104 88-89 0.07910335140653402 1104 90-91 0.2527011003887516 1104 92-93 0.29415187940071164 1104 94-95 0.5613881221346944 1104 96-97 0.6526855533285847 1104 98-99 1.0001274757724232 1104 100-101 1.5930640463952201 1105 1 0.19638569992732613 1105 2 0.1268095913170697 1105 3 0.03876884791424828 1105 4 -0.006015249214016194 1105 5 -0.11224548233014531 1105 6 -0.07892632438846903 1105 7 0.06423114971151023 1105 8 -0.0027349804223248952 1105 9 0.16482759549497672 1105 10-11 0.10416350786365314 1105 12-13 -0.0015098101349693138 1105 14-15 0.1866310230468926 1105 16-17 0.14464027893088627 1105 18-19 0.07954634922840143 1105 20-21 0.1392814278552308 1105 22-23 0.24262880849639146 1105 24-25 0.22201932528390245 1105 26-27 0.2626037656490041 1105 28-29 0.16323738885278516 1105 30-31 0.13106554765873568 1105 32-33 -0.04993331483367314 1105 34-35 0.023341696857606564 1105 36-37 -0.1286014769735928 1105 38-39 -0.21551134334943356 1105 40-41 -0.18507375532217196 1105 42-43 -0.18472435669238507 1105 44-45 -0.15330380742474858 1105 46-47 -0.12662108324044397 1105 48-49 -0.3341902054449193 1105 50-51 -0.5048493025143301 1105 52-53 -0.307096287657707 1105 54-55 -0.4949623631322879 1105 56-57 -0.24305303790327315 1105 58-59 -0.296357267460877 1105 60-61 -0.2381896010568525 1105 62-63 -0.3560418302543269 1105 64-65 -0.2049569817716943 1105 66-67 -0.23993528060280056 1105 68-69 -0.4767305098804968 1105 70-71 -0.3481831079025888 1105 72-73 -0.33830594298924055 1105 74-75 -0.3510614325627728 1105 76-77 -0.49558604391568295 1105 78-79 -0.3967351950233855 1105 80-81 -0.3638355430387783 1105 82-83 -0.21658997276457015 1105 84-85 -0.07889059413031418 1105 86-87 -0.07623381161986842 1105 88-89 -0.14180618974678438 1105 90-91 -0.018183458186626922 1105 92-93 0.28430896283813567 1105 94-95 0.22696234888007893 1105 96-97 0.3006448711727181 1105 98-99 0.6796645529861216 1105 100-101 0.7746397238832863 1106 1 -0.11271474364473022 1106 2 0.10774850153462268 1106 3 -0.19760245685471745 1106 4 -0.03477847539085843 1106 5 -1.7377953304276161E-4 1106 6 -0.09861925578073283 1106 7 -0.05815689084181486 1106 8 -0.07297844791776242 1106 9 0.06231049247271869 1106 10-11 0.07111690874310028 1106 12-13 -0.00774964694663538 1106 14-15 0.16708322278581988 1106 16-17 -0.013985641970236884 1106 18-19 0.14975613988676884 1106 20-21 0.10421822765766109 1106 22-23 0.0716923999867447 1106 24-25 -0.03194411770763139 1106 26-27 -0.16849182204387603 1106 28-29 -0.0839097019495938 1106 30-31 -0.06712825131299383 1106 32-33 -0.2145232432142663 1106 34-35 -0.5094258656129256 1106 36-37 -0.4472677459328551 1106 38-39 -0.6743686722310045 1106 40-41 -0.46213870671527246 1106 42-43 -0.30354505800450227 1106 44-45 -0.39999946824448074 1106 46-47 -0.6610965424481705 1106 48-49 -0.6042784387580511 1106 50-51 -1.1087567778099654 1106 52-53 -0.8391253652010278 1106 54-55 -1.024059150362028 1106 56-57 -0.9867554693709124 1106 58-59 -1.0905356648924496 1106 60-61 -0.9212256160286145 1106 62-63 -0.8194583614118187 1106 64-65 -1.0414294774177648 1106 66-67 -0.924499582514045 1106 68-69 -1.2115278234310942 1106 70-71 -1.1553190693121387 1106 72-73 -1.022589604591552 1106 74-75 -0.9913647486086639 1106 76-77 -1.1119395159975873 1106 78-79 -1.198748268050803 1106 80-81 -1.2026437678845845 1106 82-83 -1.3074308352764774 1106 84-85 -1.1003796234997196 1106 86-87 -0.860231380472964 1106 88-89 -0.8363340244544553 1106 90-91 -0.8617216749820678 1106 92-93 -0.8864406194087167 1106 94-95 -0.8802534747430997 1106 96-97 -0.5763168045866927 1106 98-99 -0.3361338927476645 1106 100-101 0.2800232068379991 1107 1 -0.013560716062592348 1107 2 0.05128209474718659 1107 3 -0.023461532427155873 1107 4 0.08011162190006615 1107 5 -0.2727193948899398 1107 6 -0.010901825309346691 1107 7 0.058948458521044245 1107 8 -0.17314387524439212 1107 9 0.011445684270150025 1107 10-11 0.1103956619779396 1107 12-13 -0.026786073716380088 1107 14-15 0.04047399161341758 1107 16-17 0.0011833068291835502 1107 18-19 -0.10693465842292937 1107 20-21 0.11499557230337842 1107 22-23 -0.029822242788505093 1107 24-25 0.05013486155663571 1107 26-27 0.028505917661959757 1107 28-29 -0.13589442039555877 1107 30-31 -0.04150339462874797 1107 32-33 -0.11499045256991991 1107 34-35 -0.17575812885529984 1107 36-37 -0.3480093025876201 1107 38-39 -0.43113522315313446 1107 40-41 -0.3784853015329688 1107 42-43 -0.30471205770967913 1107 44-45 -0.5108071139351864 1107 46-47 -0.5840458987556971 1107 48-49 -0.5954215252061061 1107 50-51 -0.6614457908148452 1107 52-53 -0.8840630433220369 1107 54-55 -0.8316864147509335 1107 56-57 -0.6851188034686047 1107 58-59 -0.8296579992194424 1107 60-61 -0.6045633170801423 1107 62-63 -0.9034106823080705 1107 64-65 -0.920194586992892 1107 66-67 -0.9296200496415032 1107 68-69 -0.6757214061611343 1107 70-71 -0.643344014442679 1107 72-73 -0.8628969554612738 1107 74-75 -1.0158430792667694 1107 76-77 -1.0466878535348485 1107 78-79 -0.9486929891174007 1107 80-81 -1.0799306778331577 1107 82-83 -1.1225166609390023 1107 84-85 -1.0969605191003353 1107 86-87 -0.8918734514265445 1107 88-89 -0.7212821850991276 1107 90-91 -0.6487380866831849 1107 92-93 -0.4595971693481147 1107 94-95 -0.6291642750182653 1107 96-97 -0.35687172318334603 1107 98-99 -0.08176685700880881 1107 100-101 0.6639210677480101 1108 1 0.01929980952009913 1108 2 0.15488967205241977 1108 3 -0.05819084805535368 1108 4 -0.04356900458954982 1108 5 -0.1799840743711698 1108 6 -0.0012367372479360483 1108 7 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13 4266.0 14 5375.0 15 7969.0 16 11262.0 17 16704.0 18 14901.0 19 8362.0 20 6688.0 21 6879.0 22 6766.0 23 7262.0 24 7918.0 25 9027.0 26 10532.0 27 12755.0 28 15481.0 29 19055.0 30 23712.0 31 29976.0 32 37891.0 33 50519.0 34 70154.0 35 103844.0 36 155216.0 37 220735.0 38 150273.0 39 10730.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.77460990362182 19.982441418849767 15.833862839348683 20.409085838179728 2 15.764655459968505 21.86983655390153 39.38211246518916 22.983395520940807 3 19.102908207988456 26.272098579640218 30.808076890257617 23.816916322113705 4 12.871574186842086 17.789889924056133 36.63612880204172 32.70240708706007 5 12.826664830512996 38.665614110970274 33.53198059237292 14.975740466143817 6 30.468792422679215 37.56390067565995 16.582467749067188 15.38483915259365 7 27.147314814096546 35.11725143703796 20.207255393255814 17.528178355609683 8 26.896851316024232 34.10531296786154 19.53460139165781 19.46323432445641 9 27.225954340591567 16.567147101488626 20.50193544383334 35.70496311408646 10-11 24.68936902203846 27.712918214892447 26.107644033480465 21.490068729588632 12-13 26.37952184010589 25.477165949895515 27.40677530557724 20.736536904421353 14-15 23.26474272948003 26.226039670780615 27.0354159070987 23.47380169264066 16-17 20.753998882950253 29.436151686046962 27.93244951943589 21.877399911566894 18-19 20.618391758655196 26.924292729092166 32.320602974144954 20.136712538107687 20-21 21.45543454704391 25.732955875985965 33.012261869034454 19.79934770793567 22-23 21.526524956522625 25.582975001539342 32.78133510329604 20.109164938641992 24-25 21.299138403746397 25.874553408446854 32.70259826711084 20.12370992069591 26-27 20.3845258758618 26.42246938241198 32.8824168258555 20.310587915870723 28-29 20.4300762466073 26.302849235740005 33.05062064912888 20.216453868523814 30-31 20.594425479367175 26.315608298994984 32.56525982479081 20.52470639684703 32-33 20.397793826748686 26.545979396637993 32.464791600406485 20.59143517620684 34-35 21.12799722289021 26.28834040539597 32.435604797108084 20.148057574605733 36-37 21.354084093911737 26.075027574754856 32.12809231179472 20.442796019538683 38-39 20.72641202069645 26.623503812707995 32.13568857583915 20.51439559075641 40-41 20.98613578360264 26.37279789932589 32.25035101736857 20.390715299702897 42-43 20.812410903907136 26.668266759762993 31.715251311591462 20.80407102473841 44-45 20.847280294156434 27.07274786480595 31.35632335487274 20.723648486164873 46-47 21.42199851060034 27.339162290262276 30.569540225426845 20.669298973710546 48-49 21.297784905865285 27.504150150103563 30.064201270644087 21.133863673387065 50-51 21.681382892072826 27.891141950648123 29.71667468253291 20.710800474746144 52-53 21.74271396544903 27.897056884206933 29.605454538402466 20.754774611941574 54-55 21.606912908906146 28.23537169054619 29.406189153757246 20.751526246790423 56-57 21.50926802212379 28.430370565739153 29.20323905640326 20.8571223557338 58-59 21.27504634980723 27.788406342360233 29.374481231237056 21.56206607659548 60-61 21.296718278502222 28.251613516301944 29.54024482006966 20.91142338512617 62-63 21.352716215063104 28.3139142509173 29.814998332182668 20.518371201836928 64-65 21.321638572348363 28.141217584224776 29.676045876612545 20.861097966814313 66-67 21.18001954837058 28.240413928989767 29.522403053269308 21.05716346937034 68-69 21.498650231555104 28.366178991707457 29.394456252763533 20.740714523973907 70-71 21.561969110471566 28.315562675023852 28.89081420514929 21.231654009355292 72-73 21.462890543563528 28.432773805933753 28.533133841500955 21.57120180900176 74-75 21.338365228723692 29.00130710335037 28.389305412261173 21.27102225566476 76-77 21.647735647074338 28.148247628208612 28.943272878187276 21.260743846529774 78-79 21.491523221447355 28.14572650898682 28.962666102970264 21.40008416659556 80-81 21.515376887930433 28.72335370914817 28.473326558633477 21.28794284428792 82-83 21.67618875850152 28.475515036565994 28.49713852413395 21.351157680798536 84-85 21.310608275408 28.53785740016455 28.376408255917738 21.77512606850971 86-87 21.315141842046064 29.001113171102546 28.133169395939834 21.550575590911556 88-89 21.492250467376717 28.552208500438287 28.141993313216098 21.813547718968902 90-91 21.229664626341147 28.49502719141046 28.253756759063158 22.021551423185233 92-93 21.308510720824152 28.261143606292862 28.50123731653582 21.929108356347164 94-95 21.116409771082374 28.026555142695347 28.60093397770555 22.25610110851673 96-97 21.19611592494046 27.985102124721706 28.577177277346387 22.241604672991443 98-99 20.946808159909082 28.07678956177045 28.234408301867763 22.7419939764527 100-101 20.693927227588347 28.423426044454793 28.509979052717327 22.372667675239537 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2109.0 1 1450.0 2 894.0 3 1009.5 4 880.0 5 922.5 6 1714.0 7 3379.5 8 4813.0 9 5181.0 10 4520.5 11 3757.5 12 3685.5 13 3723.0 14 3722.0 15 3702.5 16 3583.0 17 3637.0 18 3768.0 19 3889.5 20 4164.0 21 4400.0 22 4723.5 23 5176.0 24 5856.0 25 6648.5 26 7728.0 27 9145.0 28 10153.5 29 11474.5 30 13244.0 31 15072.5 32 17718.0 33 19568.5 34 20391.5 35 22028.0 36 25503.0 37 28375.0 38 29269.5 39 29290.5 40 28876.0 41 28792.5 42 27975.0 43 29483.0 44 32069.5 45 32818.5 46 32852.5 47 33873.0 48 37452.5 49 36984.5 50 34331.0 51 32824.0 52 30590.0 53 30565.0 54 31688.5 55 30420.5 56 27403.0 57 24783.5 58 23047.5 59 21161.5 60 17402.5 61 14272.5 62 11982.5 63 8538.0 64 5741.0 65 4227.5 66 3116.0 67 2459.5 68 1885.5 69 1330.0 70 943.0 71 605.5 72 447.5 73 344.5 74 234.0 75 194.5 76 132.0 77 76.5 78 54.5 79 37.0 80 21.0 81 15.5 82 10.0 83 5.0 84 3.0 85 1.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04392565413347193 2 0.0 3 0.0 4 0.0 5 0.014254020215497513 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004169543328342811 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.4544918587242362E-4 22-23 4.3634755761727084E-4 24-25 4.3634755761727084E-4 26-27 0.0017453902304690834 28-29 0.002812017593533523 30-31 6.30279805447169E-4 32-33 0.0 34-35 0.0 36-37 4.8483061957474534E-5 38-39 0.0 40-41 0.0 42-43 0.00950268014366501 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 9.696612391494907E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.9393224782989814E-4 84-85 3.3938143370232176E-4 86-87 0.0 88-89 0.0 90-91 0.007757289913195926 92-93 0.002327186973958778 94-95 0.0 96-97 0.0 98-99 1.9393224782989814E-4 100-101 0.01241166386111348 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1031288.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.94158245979801 #Duplication Level Percentage of deduplicated Percentage of total 1 67.78084462933369 27.75055039586623 2 14.945647122582145 12.237968881684797 3 6.177697212080167 7.587740994601335 4 3.3158125193954695 5.430184467362409 5 1.9773040380592328 4.047697816114684 6 1.2733697829473671 3.128026438221284 7 0.8948733275054093 2.564627109039559 8 0.6302214369955943 2.064181034455002 9 0.4562810949083205 1.6812783064832335 >10 2.293103124158445 16.73022014338731 >50 0.16000038957405258 4.489908758056785 >100 0.08766007080746706 6.949701823544752 >500 0.0045506593800151585 1.2717210022155878 >1k 0.0019160671073748033 1.2858414297211045 >5k 4.790167768437008E-4 1.6320860791792926 >10k+ 2.395083884218504E-4 1.1482653200666617 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11710 1.1354733110440536 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9581 0.929032433229127 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7063 0.6848717332112852 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2885 0.2797472674946281 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2123 0.20585908107143688 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2037 0.19751999441475127 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1799 0.1744420569229934 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1113 0.10792329591733832 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1077 0.10443251545640016 No Hit TCCCAGGACCGAGTGGGAGTAGGGGATTACTGAGTGGCATCCCCAAGCTG 1064 0.10317195584550581 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 1.454491858724236E-4 16-17 0.0 0.0 0.0 0.0 1.9393224782989814E-4 18-19 0.0 0.0 0.0 0.0 1.9393224782989814E-4 20-21 0.0 0.0 0.0 1.4544918587242362E-4 1.9393224782989814E-4 22-23 0.0 0.0 0.0 5.817967434896944E-4 4.3634755761727084E-4 24-25 0.0 0.0 0.0 0.0013090426728518125 6.787628674046435E-4 26-27 0.0 0.0 0.0 0.0022302208500438285 6.787628674046435E-4 28-29 0.0 0.0 0.0 0.00470285700987503 6.787628674046435E-4 30-31 0.0 0.0 0.0 0.009890544639324806 8.242120532770672E-4 32-33 0.0 0.0 0.0 0.017502385366648308 9.696612391494907E-4 34-35 0.0 0.0 0.0 0.023271869739587777 0.0010666273630644397 36-37 0.0 0.0 0.0 0.03025343066146411 0.0010666273630644397 38-39 0.0 0.0 0.0 0.03704105933551055 0.0010666273630644397 40-41 0.0 0.0 0.0 0.0468346378509204 0.0010666273630644397 42-43 0.0 0.0 0.0 0.059876581517481056 0.0011151104250219143 44-45 0.0 0.0 0.0 0.07301549130795665 0.001163593486979389 46-47 0.0 0.0 0.0 0.08964518155937043 0.001163593486979389 48-49 0.0 0.0 0.0 0.10637183793469913 0.001260559610894338 50-51 0.0 0.0 0.0 0.1255226474079016 0.001260559610894338 52-53 0.0 0.0 0.0 0.17129065789575754 0.0013090426728518125 54-55 0.0 0.0 0.0 0.23010061205017415 0.001357525734809287 56-57 0.0 0.0 0.0 0.283722878575141 0.001357525734809287 58-59 9.696612391494907E-5 0.0 0.0 0.37889512919766355 0.001357525734809287 60-61 9.696612391494907E-5 0.0 0.0 0.49404240134666555 0.001357525734809287 62-63 1.454491858724236E-4 0.0 0.0 0.5754454623732652 0.001454491858724236 64-65 1.9393224782989814E-4 0.0 0.0 0.6448247240344114 0.001454491858724236 66-67 1.9393224782989814E-4 0.0 0.0 0.8094247193800375 0.001454491858724236 68-69 1.9393224782989814E-4 0.0 0.0 0.9767882492572395 0.001454491858724236 70-71 1.9393224782989814E-4 0.0 0.0 1.0588215900892863 0.0015029749206817106 72-73 1.9393224782989814E-4 0.0 0.0 1.2338939268177271 0.0017453902304690834 74-75 1.9393224782989814E-4 0.0 0.0 1.481351475048677 0.0017453902304690834 76-77 1.9393224782989814E-4 0.0 0.0 1.7186275802685573 0.0017453902304690834 78-79 1.9393224782989814E-4 0.0 0.0 1.9781574109269187 0.0017453902304690834 80-81 1.9393224782989814E-4 0.0 0.0 2.2714314527076818 0.0017453902304690834 82-83 1.9393224782989814E-4 0.0 0.0 2.534112682393279 0.0018423563543840324 84-85 1.9393224782989814E-4 0.0 0.0 2.807169287337776 0.0018423563543840324 86-87 1.9393224782989814E-4 0.0 0.0 3.2316384947754653 0.0018423563543840324 88-89 1.9393224782989814E-4 0.0 0.0 3.779303162647097 0.0018423563543840324 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6190 0.0 36.702908 1 GTATCAA 9410 0.0 33.083763 1 GTAAGGT 480 0.0 29.690092 4 GGTAAGG 485 0.0 29.384007 3 AGGTAAG 410 0.0 27.807304 2 TAAGGTG 635 0.0 27.679583 5 CCTATCG 70 0.008387604 27.145227 3 TATCAAC 12015 0.0 25.857439 2 ATCAACG 12035 0.0 25.735525 3 TCAACGC 12125 0.0 25.544498 4 CAACGCA 12300 0.0 25.258303 5 AACGCAG 12550 0.0 24.713701 6 GTACATG 6410 0.0 23.727674 1 GGTGTGC 710 0.0 23.41867 8 GAGTACT 8100 0.0 23.225376 12-13 TACATGG 6520 0.0 22.877764 2 ACATGGG 6225 0.0 22.588318 3 ACGCAGA 13755 0.0 22.449432 7 CGCAGAG 13860 0.0 22.210808 8 AGTACTT 8385 0.0 22.209335 12-13 >>END_MODULE