FastQCFastQC Report
Thu 26 May 2016
SRR937811_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937811_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151494
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16741.104994257198305No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13640.9003656910504707No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13420.8858436637754631No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11760.7762683670640421No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11720.7736279984685862No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT9040.5967233025730392No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7870.5194925211559533No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC5800.38285344634110924No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5430.3584300368331419No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG5340.3524892074933661No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA4990.3293859822831267No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT4840.31948460005016704No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC4530.29902174343538357No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4430.29242082194674374No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT4340.28647999260696794No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.2811992554160561No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA4160.2745983339274163No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC3700.2442340950796731No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3540.23367262069784941No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT3390.22377123846488972No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG3380.22311114631602572No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG3100.20462856614783423No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA3080.20330838185010627No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG2940.19406709176601053No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.1920868153194186No Hit
GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG2710.17888497234213896No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC2670.17624460374668305No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC2610.17228405085349915No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC2380.15710193142962758No Hit
GTTCCAGTAGTGACTGATTCACACTCTTTTCCAAGTGCAGTGCACACTCC2150.141919812005756No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG2060.13597898266598019No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1980.1306982454750683No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.1306982454750683No Hit
GATAATACGTCTCAATGAAGTCACATAAGTGGGGATCATTCTTGTCAGTA1970.13003815332620433No Hit
CCCTGGGACACGGTGATGAGAGCTAAGCTGACTTCCCCAAAGCCACGTGA1860.12277713968870053No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC1820.12013677109324462No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.11881658679551667No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG1590.10495465166937305No Hit
CTCCATTGCATTCAGCCCGCTCTCCCAGTCATCACGGTCTGGTTTCTTTA1590.10495465166937305No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG1580.10429455952050908No Hit
AGATGATGTGGCTCTGAAGAACTTTGCCAAATACTTTCTCCACCAATCTC1530.10099409877618916No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCT250.00161376738.00079312-13
GTAAGCG250.00161507937.99450794-95
TAAGGCG352.0535027E-433.9180646-47
CAGGGAG600.00391432531.7092845
TTTGGAC650.005808814429.2507323
AGGGCGT1056.8659145E-527.1434238
AATGCTA350.00838787427.13893318-19
GAGTACT9850.025.5589112-13
GTACTTT10050.024.81395314-15
TGGACAG1000.001634179123.7819635
TGCACCG1000.001634179123.7819635
TACCCCA1050.002170426522.6494885
AAGACTT1050.002170426522.6494885
CTTGAAG1050.00217880322.6344953
CACCGTC1050.002187206222.6195187
ACTTTTT11300.022.27922616-17
ACATAAT652.9341574E-421.92353678-79
GCACCGT1100.002865596421.591366
TGGGGAG1100.002865596421.591366
ACCCCAT1100.002865596421.591366
Produced by FastQC (version 0.11.5)