##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937811_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 151494 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.69077323194318 33.0 31.0 34.0 30.0 34.0 2 31.8080187994244 33.0 31.0 34.0 30.0 34.0 3 31.798414458658428 33.0 31.0 34.0 30.0 34.0 4 35.40475530384042 37.0 35.0 37.0 33.0 37.0 5 35.374760716596036 37.0 35.0 37.0 33.0 37.0 6 35.46407778525882 37.0 35.0 37.0 33.0 37.0 7 35.42360753561197 37.0 35.0 37.0 33.0 37.0 8 35.42441944895507 37.0 35.0 37.0 33.0 37.0 9 37.047255996937174 39.0 37.0 39.0 33.0 39.0 10-11 36.91250478566808 39.0 37.0 39.0 33.0 39.0 12-13 36.80413085666759 39.0 37.0 39.0 33.0 39.0 14-15 37.89149735303049 40.0 38.0 41.0 33.0 41.0 16-17 37.80116704291919 40.0 38.0 41.0 33.0 41.0 18-19 37.73762657266954 40.0 37.5 41.0 33.0 41.0 20-21 37.63581726008951 40.0 37.0 41.0 32.5 41.0 22-23 37.54181353716979 40.0 37.0 41.0 32.0 41.0 24-25 37.51601383553144 40.0 37.0 41.0 32.0 41.0 26-27 37.363053982335934 40.0 37.0 41.0 32.0 41.0 28-29 37.238867545909415 39.5 36.5 41.0 32.0 41.0 30-31 36.96560589858345 39.0 36.0 41.0 31.5 41.0 32-33 36.710387870146675 39.0 35.0 41.0 31.0 41.0 34-35 36.63811438076756 39.0 35.0 41.0 31.0 41.0 36-37 36.44311985953239 39.0 35.0 41.0 30.5 41.0 38-39 36.23509181881791 39.0 35.0 41.0 30.0 41.0 40-41 35.96924630678443 39.0 35.0 40.5 30.0 41.0 42-43 35.60108321121629 38.0 35.0 40.0 28.5 41.0 44-45 35.251313583376245 38.0 35.0 40.0 27.0 41.0 46-47 34.728735131424344 38.0 34.5 40.0 24.5 41.0 48-49 34.23398946492931 38.0 34.0 40.0 23.0 41.0 50-51 33.21020634480574 37.0 32.5 39.5 21.0 40.5 52-53 33.23408187783015 37.0 33.0 39.5 20.0 40.5 54-55 33.42451186185592 37.0 33.0 40.0 18.5 41.0 56-57 33.08035631774196 37.0 33.0 40.0 15.5 41.0 58-59 32.78268116229026 36.0 33.0 40.0 12.0 41.0 60-61 32.36373387724926 36.0 32.0 39.0 8.0 41.0 62-63 31.925254465523388 35.0 31.5 39.0 4.5 41.0 64-65 31.49111515967629 35.0 31.0 39.0 2.0 40.0 66-67 30.974401626467056 35.0 31.0 38.0 2.0 40.0 68-69 30.449153101773007 35.0 30.0 37.0 2.0 39.5 70-71 29.904501168363105 35.0 29.5 37.0 2.0 39.0 72-73 29.436231797958996 34.0 29.0 36.0 2.0 39.0 74-75 28.935931456031263 34.0 29.0 35.5 2.0 38.0 76-77 28.494263799226374 34.0 28.0 35.0 2.0 37.0 78-79 27.945341069613317 34.0 26.5 35.0 2.0 37.0 80-81 27.37551322164574 33.0 26.0 35.0 2.0 36.0 82-83 26.90862344383276 33.0 25.0 35.0 2.0 36.0 84-85 26.432502277317916 33.0 24.5 35.0 2.0 35.0 86-87 25.990375856469562 32.5 23.5 35.0 2.0 35.0 88-89 25.52204377731131 32.0 21.0 35.0 2.0 35.0 90-91 25.008472282730672 32.0 18.5 34.0 2.0 35.0 92-93 24.427990547480427 31.5 14.0 34.0 2.0 35.0 94-95 23.70092874965345 31.0 4.5 34.0 2.0 35.0 96-97 22.871357941568643 31.0 2.0 34.0 2.0 35.0 98-99 21.904227230121325 30.0 2.0 34.0 2.0 35.0 100-101 19.28650969675367 26.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.3082605946558985 1101 2 0.1937324718864737 1101 3 -0.08768371138770803 1101 4 -0.2133121851222839 1101 5 0.23978037819519926 1101 6 -0.029160837461922995 1101 7 0.1445679684330372 1101 8 0.25946076836581966 1101 9 0.34461227484192136 1101 10-11 0.3880612372990768 1101 12-13 0.4524524774570793 1101 14-15 0.500645723561199 1101 16-17 0.3866008706104225 1101 18-19 0.6920025787305661 1101 20-21 0.1579935308347018 1101 22-23 0.323801597974672 1101 24-25 0.07057557564444039 1101 26-27 -0.23065115001994485 1101 28-29 -0.24938987137529978 1101 30-31 0.02101219641187413 1101 32-33 -0.18873234377768 1101 34-35 -0.432498219406213 1101 36-37 -0.46011380703276217 1101 38-39 -0.5628377098088038 1101 40-41 0.016287304860831853 1101 42-43 -0.35843070011243583 1101 44-45 0.11549041414534145 1101 46-47 0.09612653507736724 1101 48-49 0.015870933424814382 1101 50-51 -0.16576039337771675 1101 52-53 -0.8308634786311586 1101 54-55 -0.7594895952302139 1101 56-57 -0.21429492141383832 1101 58-59 -0.5986344929501897 1101 60-61 -0.13892419400969658 1101 62-63 -0.4087950182182212 1101 64-65 0.17034051791308968 1101 66-67 0.029178079194313256 1101 68-69 -0.5233060329420667 1101 70-71 -0.6412640834673056 1101 72-73 0.02044350617262225 1101 74-75 -0.011469443014956227 1101 76-77 -0.5101127428494117 1101 78-79 -0.02287921291077666 1101 80-81 0.3419069007893256 1101 82-83 0.3581827969435203 1101 84-85 0.04010315674922538 1101 86-87 1.1875207137591772 1101 88-89 0.6987968336079255 1101 90-91 1.2666828478270595 1101 92-93 1.6311098734622007 1101 94-95 0.6381281824804752 1101 96-97 0.3379518434958264 1101 98-99 0.6551154549973113 1101 100-101 0.4995918807668538 1102 1 0.2519975347440351 1102 2 0.5867512449337404 1102 3 0.4047735464064566 1102 4 0.42840756010064496 1102 5 -0.0667482842251843 1102 6 0.1984204565374057 1102 7 0.16945901771228478 1102 8 0.052119165952142055 1102 9 0.24798136636052703 1102 10-11 0.18405101269298285 1102 12-13 0.2299625344466918 1102 14-15 0.4165441479661638 1102 16-17 0.5098408974290578 1102 18-19 0.16417824081571553 1102 20-21 0.3920071077706808 1102 22-23 0.7592273438680692 1102 24-25 0.45121168023712244 1102 26-27 0.5364247802515933 1102 28-29 0.6077589720708971 1102 30-31 0.8274151464621582 1102 32-33 1.1590718801579527 1102 34-35 1.3006353709390766 1102 36-37 1.4096691296081971 1102 38-39 1.1665454614952537 1102 40-41 0.7840127490445425 1102 42-43 1.5243844188952806 1102 44-45 1.9921246749566066 1102 46-47 1.942589827065298 1102 48-49 1.514949042710768 1102 50-51 1.3749494624720953 1102 52-53 1.498523883085241 1102 54-55 1.60907812183315 1102 56-57 1.612117247543587 1102 58-59 0.9610160266609427 1102 60-61 1.337295215980241 1102 62-63 1.206944170517943 1102 64-65 1.2691504408095042 1102 66-67 1.294808317209739 1102 68-69 1.3748878993408695 1102 70-71 0.9833797314840851 1102 72-73 1.0158550046640755 1102 74-75 0.7408562358318385 1102 76-77 1.0535815246665088 1102 78-79 0.7729429459896586 1102 80-81 0.9488043194953271 1102 82-83 -0.03988542296931996 1102 84-85 1.055712442702962 1102 86-87 1.241597984962663 1102 88-89 1.1365482583481175 1102 90-91 0.8466359152088891 1102 92-93 1.8256288141259596 1102 94-95 1.975980177116746 1102 96-97 1.4489768183533513 1102 98-99 1.075644285034187 1102 100-101 1.5069670064792184 1103 1 0.1661976096784663 1103 2 -0.009268904102516018 1103 3 -0.07942777743517837 1103 4 0.2948577495182363 1103 5 -0.025742497621791927 1103 6 0.19009440849610826 1103 7 0.2189573733177923 1103 8 -0.1177322140903172 1103 9 0.07380044133659425 1103 10-11 -0.042042821868442104 1103 12-13 0.2545035610484163 1103 14-15 0.17423533484431175 1103 16-17 0.10405013101633642 1103 18-19 0.1954425512107889 1103 20-21 0.18183684008823775 1103 22-23 -0.09729403326027608 1103 24-25 0.3212420703124792 1103 26-27 0.35945462913382187 1103 28-29 0.031096884730651198 1103 30-31 -0.04159530272814038 1103 32-33 0.14735296753982396 1103 34-35 0.25194880501759087 1103 36-37 0.23865210283995708 1103 38-39 0.2822775292692796 1103 40-41 0.6731930496148735 1103 42-43 0.48614704326561764 1103 44-45 0.41304803368439025 1103 46-47 -0.1125077958479821 1103 48-49 0.3013026499710776 1103 50-51 0.062202282920864604 1103 52-53 0.14654020213939845 1103 54-55 0.10503998853312169 1103 56-57 0.21086417731337548 1103 58-59 -0.04129445167052381 1103 60-61 0.3071757371908532 1103 62-63 0.7705135473132572 1103 64-65 1.1429067373833348 1103 66-67 0.930751866604016 1103 68-69 0.7708331139447608 1103 70-71 0.964708568751476 1103 72-73 0.5404168463859023 1103 74-75 0.168419617872555 1103 76-77 0.8787761460394776 1103 78-79 0.5724725242753266 1103 80-81 1.1082897697263725 1103 82-83 0.8889060511574876 1103 84-85 0.7197772193567253 1103 86-87 0.6901221929473422 1103 88-89 0.16837457698597547 1103 90-91 0.37916994793025793 1103 92-93 1.0520765057291435 1103 94-95 1.4819749317064783 1103 96-97 1.107796184741094 1103 98-99 1.3799821560637042 1103 100-101 1.0625433771548778 1104 1 0.4550775452143263 1104 2 0.7783575620090453 1104 3 0.6960221285546133 1104 4 0.5092905546037443 1104 5 0.4366981864143753 1104 6 0.6949739858978461 1104 7 0.5672428062124126 1104 8 0.740715274494157 1104 9 0.7665661320877746 1104 10-11 1.11296210247572 1104 12-13 0.6320216915882995 1104 14-15 0.898897345767395 1104 16-17 0.7145568907978728 1104 18-19 0.8279074093001384 1104 20-21 1.1347869699118505 1104 22-23 1.0400056354656542 1104 24-25 0.5937821365672846 1104 26-27 0.9288207317392434 1104 28-29 1.0383714840681009 1104 30-31 0.5139826362099953 1104 32-33 0.8282106987601665 1104 34-35 0.7947458325043897 1104 36-37 0.6223032802058768 1104 38-39 0.8596568828372 1104 40-41 1.0673868001744609 1104 42-43 0.9407311600173429 1104 44-45 0.8678336008793011 1104 46-47 0.7709643144573235 1104 48-49 0.2828139759626609 1104 50-51 -0.047744892296250896 1104 52-53 0.01326954227727839 1104 54-55 -0.010435882180459544 1104 56-57 0.48230745782192486 1104 58-59 0.30795346667490264 1104 60-61 0.6280639098114946 1104 62-63 -0.3375981905325709 1104 64-65 0.6716022338467624 1104 66-67 0.8090086487689341 1104 68-69 0.7916272763585823 1104 70-71 1.1684421169652808 1104 72-73 1.2338988053795426 1104 74-75 1.5560774207197205 1104 76-77 1.4624899968766165 1104 78-79 1.8399091793026336 1104 80-81 1.584787218020761 1104 82-83 2.2197995957899543 1104 84-85 2.321330626107553 1104 86-87 2.3184237418774174 1104 88-89 2.0887121183952324 1104 90-91 1.562825601972694 1104 92-93 1.3301005007152646 1104 94-95 0.6507904031005189 1104 96-97 0.9494785197755675 1104 98-99 0.7172549647305502 1104 100-101 0.9667872664914334 1105 1 0.20527323993056612 1105 2 0.2988076598563971 1105 3 0.25404561192056363 1105 4 0.22240210059199939 1105 5 0.4147803781951964 1105 6 -0.03310820588297503 1105 7 0.2047183443728855 1105 8 0.3632201668620638 1105 9 0.17468746281184622 1105 10-11 0.4178544703817906 1105 12-13 0.4948396955021934 1105 14-15 -0.11157232155158425 1105 16-17 0.22776628414425204 1105 18-19 0.37546122534710946 1105 20-21 0.34887698948132595 1105 22-23 0.36759859045587717 1105 24-25 0.23683497414067745 1105 26-27 0.32696163193494243 1105 28-29 -0.02420190145048906 1105 30-31 0.3027791137051068 1105 32-33 0.37874885922983736 1105 34-35 0.655753660293044 1105 36-37 1.0724989749221265 1105 38-39 0.8647938691385662 1105 40-41 0.8657233950863983 1105 42-43 0.458392608158249 1105 44-45 0.7025204893333097 1105 46-47 1.1965024749269944 1105 48-49 1.2333521364323303 1105 50-51 1.7375290803064942 1105 52-53 1.565471107835009 1105 54-55 1.9563938634164089 1105 56-57 1.477904326706458 1105 58-59 1.1601061085535633 1105 60-61 1.3357938511030838 1105 62-63 1.386129793811854 1105 64-65 1.7301149540033123 1105 66-67 1.8413961243070993 1105 68-69 1.2156977264564333 1105 70-71 1.097551706006378 1105 72-73 1.565650273089915 1105 74-75 1.6544140156316622 1105 76-77 1.3684586857220182 1105 78-79 1.28379371942005 1105 80-81 1.4520572767291746 1105 82-83 1.8552692631089336 1105 84-85 1.508806164268023 1105 86-87 1.8069756009772213 1105 88-89 1.7477629990214574 1105 90-91 1.552209163616535 1105 92-93 1.3390046103043076 1105 94-95 2.0538236712022773 1105 96-97 1.4455646254507108 1105 98-99 1.7918635752980592 1105 100-101 1.5775993995638409 1106 1 0.4088446685019953 1106 2 0.4470219455706861 1106 3 0.45225989763484975 1106 4 0.679544957734862 1106 5 0.5969232353380534 1106 6 0.44546322268845273 1106 7 0.5886469158014549 1106 8 0.691791595433493 1106 9 0.5264731770975644 1106 10-11 -0.06518124390392899 1106 12-13 0.18412540978790304 1106 14-15 1.0777133927341254 1106 16-17 0.38401628414425204 1106 18-19 0.3522469396328205 1106 20-21 0.6051269894813274 1106 22-23 0.2488485904558715 1106 24-25 0.8287992598549607 1106 26-27 1.010890203363516 1106 28-29 1.0132980985495124 1106 30-31 1.5581362565622499 1106 32-33 1.2323202878012651 1106 34-35 1.6048608031501814 1106 36-37 0.5716061177792682 1106 38-39 0.7531867262814274 1106 40-41 0.625544823657826 1106 42-43 0.6342854653011045 1106 44-45 0.9632347750475958 1106 46-47 0.4116810463555609 1106 48-49 0.8163878507180442 1106 50-51 0.9937790803064885 1106 52-53 0.6806496792635812 1106 54-55 0.089429577702127 1106 56-57 0.384154326706458 1106 58-59 0.8038561085535676 1106 60-61 0.4759724225316546 1106 62-63 0.5968440795261358 1106 64-65 0.3390435254318902 1106 66-67 0.32711041002137975 1106 68-69 0.43534058359928807 1106 70-71 -0.05691257970790531 1106 72-73 1.0942217016613434 1106 74-75 0.8776283013459469 1106 76-77 1.1461372571505883 1106 78-79 0.9587937194200506 1106 80-81 0.4020572767291739 1106 82-83 -0.052766451176783136 1106 84-85 -0.1260152643034047 1106 86-87 -0.1555243990227808 1106 88-89 0.030798713307170544 1106 90-91 0.9504234493308168 1106 92-93 0.8827546103043069 1106 94-95 1.2466808140594203 1106 96-97 1.0080646254507108 1106 98-99 0.6311492895837745 1106 100-101 -0.3911506004361591 1107 1 0.4698911801299026 1107 2 0.2585529533226243 1107 3 0.33826488102355157 1107 4 0.19560254355988604 1107 5 0.04888890532698298 1107 6 0.22231815070617245 1107 7 -0.17927944078769542 1107 8 0.05281042156859428 1107 9 0.375518027595902 1107 10-11 0.4154735180008302 1107 12-13 0.4724698173183626 1107 14-15 0.749778730497141 1107 16-17 0.9780431390722697 1107 18-19 0.7997137170414561 1107 20-21 0.43392682336836685 1107 22-23 0.7023715694149004 1107 24-25 0.9225492598549607 1107 26-27 1.0439051535296286 1107 28-29 0.8201225725251504 1107 30-31 0.6203594016342322 1107 32-33 1.108289280049327 1107 34-35 0.6871213236374487 1107 36-37 0.8231634267493675 1107 38-39 0.976227977665701 1107 40-41 0.569377880136237 1107 42-43 0.7573128739389716 1107 44-45 1.2697131803632118 1107 46-47 1.4812754538860204 1107 48-49 0.8807220146161612 1107 50-51 0.9915988477483495 1107 52-53 1.0413570436046626 1107 54-55 0.48252896862128125 1107 56-57 0.9474502846245159 1107 58-59 0.2519112026842407 1107 60-61 0.26940403936443147 1107 62-63 0.14944651584950464 1107 64-65 -0.05028371709303059 1107 66-67 0.13663421954518995 1107 68-69 0.13502220043206847 1107 70-71 0.5066602331381631 1107 72-73 0.07636455880420101 1107 74-75 0.4185336169605627 1107 76-77 0.6449260168405111 1107 78-79 0.8217505300512791 1107 80-81 0.7767804218011598 1107 82-83 0.6596158854788108 1107 84-85 0.7976765961617112 1107 86-87 0.6979501303238465 1107 88-89 0.017059787504290824 1107 90-91 -0.18558153405788502 1107 92-93 -0.10828221140112149 1107 94-95 0.11102189933074058 1107 96-97 1.0156089222391955 1107 98-99 1.179875756914896 1107 100-101 2.0846592002282947 1108 1 -0.1770928314980047 1108 2 0.12670944557068609 1108 3 0.11297418334913445 1108 4 0.6929378148777161 1108 5 -0.010219621804800738 1108 6 0.19657929411702213 1108 7 0.0808344158014549 1108 8 0.0176844525763471 1108 9 0.26977674852613376 1108 10-11 0.22276518466750161 1108 12-13 0.47876826693076424 1108 14-15 0.5598562498769866 1108 16-17 0.40633771271568264 1108 18-19 0.4565996182042511 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#Quality Count 2 903.0 3 171.0 4 120.0 5 161.0 6 177.0 7 188.0 8 250.0 9 297.0 10 378.0 11 444.0 12 665.0 13 898.0 14 1082.0 15 1339.0 16 1644.0 17 2287.0 18 1921.0 19 1400.0 20 1600.0 21 1588.0 22 1675.0 23 1681.0 24 1923.0 25 2136.0 26 2406.0 27 2729.0 28 3095.0 29 3676.0 30 4390.0 31 5539.0 32 6877.0 33 9015.0 34 11972.0 35 17156.0 36 21996.0 37 24153.0 38 12835.0 39 727.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.288478091025866 18.04560562522346 15.154203689235537 21.511712594515142 2 17.095917975601846 21.518166233112915 35.90751735869788 25.47839843258736 3 20.994870097633626 23.274532516961774 30.72116498427933 25.00943240112527 4 13.918344652289608 17.093609754160212 35.18143426400307 33.80661132954711 5 14.512861927899065 36.17293103676539 32.671812320442726 16.642394714892827 6 31.449980820359517 34.09610983981693 16.163145990132406 18.29076334969114 7 27.100473532446234 33.473982169783866 20.439274093277955 18.986270204491944 8 26.263888888888886 30.353174603174605 19.805555555555557 23.577380952380953 9 25.2694998875706 18.011190032141585 21.650595875825033 35.068714204462786 10-11 26.149486104313553 24.95370309131073 25.330692204923345 23.566118599452373 12-13 27.29052311355069 22.900932478010713 26.846108061636137 22.96243634680246 14-15 24.188433545297926 25.34580650708147 26.875192206581154 23.590567741039454 16-17 21.92397361067478 28.229302733180113 27.104945518279074 22.741778137866035 18-19 21.24361411920311 26.75109531288749 29.93237992890799 22.072910639001407 20-21 23.083306882505422 25.007273977675503 29.055903824789713 22.85351531502936 22-23 22.126152696541165 24.786488693737333 30.039709961877115 23.047648647844387 24-25 23.506032741136053 25.350738841330145 29.12942701358648 22.01380140394732 26-27 22.329092327835898 24.833688219967996 31.238510269669757 21.598709182526353 28-29 20.970744724824492 26.83932580925706 30.340567513318145 21.849361952600304 30-31 21.646647970792106 25.469270860892113 30.00720937615747 22.876871792158315 32-33 22.17276274885905 25.655466631390965 30.04762219723527 22.12414842251472 34-35 23.17236909116163 25.72969648065717 28.603506776107356 22.49442765207384 36-37 22.841820574368334 25.10266023924299 30.0412624729711 22.014256713417577 38-39 22.123665601020903 25.65054533071936 29.612830066418272 22.612959001841464 40-41 21.874876013383943 26.01272267996244 29.71942655363496 22.39297475301866 42-43 22.465289603248344 26.012545010266802 27.846351729816053 23.675813656668794 44-45 22.107041769171758 25.667936407163456 28.29901066052959 23.9260111631352 46-47 23.24437110838489 26.572601045589973 28.171738648801476 22.011289197223665 48-49 23.35336073460993 25.584040685274406 28.285259854704897 22.77733872541077 50-51 21.750661375661377 26.759920634920636 28.571097883597886 22.918320105820104 52-53 22.666821015727066 27.107443483437795 26.710876647538406 23.51485885329673 54-55 21.978824992064332 25.760832187070154 27.583059993651464 24.67728282721405 56-57 22.885272783776458 25.9542968982458 27.16729371780715 23.99313660017059 58-59 23.987833504149172 25.235229940159353 27.529341752901114 23.24759480279036 60-61 23.091291382627727 25.67723038065075 29.25860051176599 21.972877724955534 62-63 21.800725368387294 25.944317892530428 28.69139443311171 23.563562305970567 64-65 21.85472939465071 26.69487883095844 28.092703408602503 23.35768836578834 66-67 22.412681620576226 26.00294229465924 27.046630411425358 24.537745673339174 68-69 21.452589327928635 25.759374183757476 28.177931777829947 24.61010471048394 70-71 23.497341832416417 25.83414885738468 27.208857913669064 23.459651396529836 72-73 22.957854507465054 24.95371533609278 28.695169203507053 23.39326095293511 74-75 21.84865336674844 27.16079587909726 27.020611126172888 23.969939627981407 76-77 22.06470170102326 25.737523349809067 27.186947250095052 25.010827699072617 78-79 22.685044368329873 25.687345438195113 27.722602060383245 23.905008133091766 80-81 23.074786840478325 26.12729070032697 26.980590003074656 23.81733245612005 82-83 22.662549549221925 25.32324771800831 28.084118792503233 23.930083940266535 84-85 22.55019934279688 25.64150032727922 27.32979827699062 24.478502052933283 86-87 22.701442951356178 25.458091601791704 27.340705112312193 24.499760334539925 88-89 22.39394620613049 25.374890904763163 26.74320964798604 25.48795324112031 90-91 22.597431794437757 24.727905100704866 27.355290477009138 25.319372627848235 92-93 23.239085087884586 24.64801536534005 27.845381307045642 24.267518239729718 94-95 22.829939169531873 24.540465485321345 27.62397513885216 25.005620206294633 96-97 21.708873313938113 24.993718592964825 28.40650621528696 24.890901877810105 98-99 22.639588202817386 25.43680718330859 26.63689049487733 25.286714118996688 100-101 22.54222721713549 23.625095031897665 27.7539417578422 26.078735993124646 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 630.0 1 419.0 2 239.5 3 267.0 4 212.5 5 201.5 6 307.0 7 494.0 8 653.0 9 690.0 10 567.0 11 412.0 12 387.0 13 396.5 14 405.5 15 415.5 16 431.0 17 447.0 18 439.0 19 428.5 20 468.5 21 529.5 22 565.5 23 652.0 24 684.0 25 671.0 26 738.0 27 807.0 28 862.0 29 977.5 30 1074.5 31 1159.5 32 1275.0 33 1317.5 34 1505.5 35 1632.0 36 1566.0 37 1662.0 38 1981.5 39 2200.0 40 2323.0 41 2954.0 42 3693.5 43 4001.0 44 4073.0 45 4219.0 46 4502.0 47 6087.0 48 9514.0 49 9724.0 50 6959.0 51 5711.5 52 5423.0 53 5419.5 54 5554.5 55 5401.0 56 4745.0 57 4116.5 58 3824.5 59 3601.5 60 3313.5 61 3024.0 62 2684.5 63 2187.5 64 1706.0 65 1366.0 66 1042.0 67 897.0 68 726.5 69 477.0 70 387.5 71 338.0 72 309.5 73 234.5 74 152.0 75 127.0 76 92.0 77 56.0 78 24.5 79 12.5 80 12.5 81 7.0 82 3.5 83 3.0 84 2.0 85 1.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 1.0 95 1.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30364238847743147 2 0.27525842607628026 3 0.2765786103740082 4 0.2772387025228722 5 0.28251943971378407 6 0.19274690746828257 7 0.19142672317055462 8 0.19406709176601053 9 0.19010653887282666 10-11 0.19538727606373849 12-13 0.18746617027737073 14-15 0.1910966770961226 16-17 0.1957173221381705 18-19 0.18581593990521075 20-21 0.17822488019327498 22-23 0.17987511056543493 24-25 0.18317557130975484 26-27 0.17954506449100296 28-29 0.19175676924498658 30-31 0.1993478289569224 32-33 0.20066801325465036 34-35 0.1986877368080584 36-37 0.17624460374668305 38-39 0.16865354403474725 40-41 0.17690469589554703 42-43 0.18383566345861882 44-45 0.18614598597964277 46-47 0.18977649279839465 48-49 0.18779621635180271 50-51 0.19406709176601053 52-53 0.21221962585977003 54-55 0.18350561738418683 56-57 0.16964368225804324 58-59 0.1709638665557712 60-61 0.1670033136625873 62-63 0.17195400477906717 64-65 0.1709638665557712 66-67 0.1660131754392913 68-69 0.176574649821115 70-71 0.17294414300236313 72-73 0.16898359010917924 74-75 0.1749244194489551 76-77 0.17261409692793114 78-79 0.17162395870463518 80-81 0.1699737283324752 82-83 0.16865354403474725 84-85 0.1630427607694034 86-87 0.1594122539506515 88-89 0.16502303721599537 90-91 0.17162395870463518 92-93 0.16139253039724344 94-95 0.16766340581145128 96-97 0.16766340581145128 98-99 0.16799345188588327 100-101 0.15116110208985176 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 151494.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.979919996831555 #Duplication Level Percentage of deduplicated Percentage of total 1 72.86521685710775 31.317411910702734 2 13.20647499692837 11.352264776162754 3 5.217164270794938 6.726999089072834 4 2.6308514559528198 4.522951404016 5 1.5527091780316993 3.336765812507426 6 0.9245607568497358 2.384252841696701 7 0.5943604865462587 1.7881896312725256 8 0.4438505958963017 1.5261330481735251 9 0.3363435311463325 1.3010416254109074 >10 1.9612360240815825 16.322758657108533 >50 0.16893967317852315 5.0186806078128505 >100 0.08293402137854773 7.751462104109734 >500 0.007679076053569234 2.20998851439661 >1k 0.007679076053569234 4.441099977556867 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1674 1.104994257198305 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1364 0.9003656910504707 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1342 0.8858436637754631 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1176 0.7762683670640421 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1172 0.7736279984685862 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 904 0.5967233025730392 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 787 0.5194925211559533 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 580 0.38285344634110924 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 543 0.3584300368331419 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 534 0.3524892074933661 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 499 0.3293859822831267 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 484 0.31948460005016704 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 453 0.29902174343538357 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 443 0.29242082194674374 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 434 0.28647999260696794 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 426 0.2811992554160561 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 416 0.2745983339274163 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 370 0.2442340950796731 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 354 0.23367262069784941 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 339 0.22377123846488972 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 338 0.22311114631602572 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 310 0.20462856614783423 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 308 0.20330838185010627 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 294 0.19406709176601053 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 291 0.1920868153194186 No Hit GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG 271 0.17888497234213896 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 267 0.17624460374668305 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 261 0.17228405085349915 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 238 0.15710193142962758 No Hit GTTCCAGTAGTGACTGATTCACACTCTTTTCCAAGTGCAGTGCACACTCC 215 0.141919812005756 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 206 0.13597898266598019 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 198 0.1306982454750683 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 198 0.1306982454750683 No Hit GATAATACGTCTCAATGAAGTCACATAAGTGGGGATCATTCTTGTCAGTA 197 0.13003815332620433 No Hit CCCTGGGACACGGTGATGAGAGCTAAGCTGACTTCCCCAAAGCCACGTGA 186 0.12277713968870053 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 182 0.12013677109324462 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 180 0.11881658679551667 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 159 0.10495465166937305 No Hit CTCCATTGCATTCAGCCCGCTCTCCCAGTCATCACGGTCTGGTTTCTTTA 159 0.10495465166937305 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 158 0.10429455952050908 No Hit AGATGATGTGGCTCTGAAGAACTTTGCCAAATACTTTCTCCACCAATCTC 153 0.10099409877618916 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 6.600921488639814E-4 5 0.0 0.0 0.0 0.0 6.600921488639814E-4 6 0.0 0.0 0.0 0.0 6.600921488639814E-4 7 0.0 0.0 0.0 0.0 6.600921488639814E-4 8 0.0 0.0 0.0 0.0 6.600921488639814E-4 9 0.0 0.0 0.0 0.0 6.600921488639814E-4 10-11 0.0 0.0 0.0 0.0 6.600921488639814E-4 12-13 0.0 0.0 0.0 0.0 6.600921488639814E-4 14-15 0.0 0.0 0.0 0.0 0.0013201842977279628 16-17 0.0 0.0 0.0 0.0 0.0013201842977279628 18-19 0.0 0.0 0.0 0.0 0.003300460744319907 20-21 0.0 0.0 0.0 0.0 0.003300460744319907 22-23 0.0 0.0 0.0 0.0019802764465919444 0.003960552893183889 24-25 0.0 0.0 0.0 0.00462064504204787 0.00462064504204787 26-27 0.0 0.0 0.0 0.007921105786367778 0.00462064504204787 28-29 0.0 0.0 0.0 0.022113086986943378 0.00462064504204787 30-31 0.0 0.0 0.0 0.039935575006270874 0.00462064504204787 32-33 0.0 0.0 0.0 0.06237870806764625 0.005280737190911851 34-35 0.0 0.0 0.0 0.09010257831993346 0.005280737190911851 36-37 0.0 0.0 0.0 0.13234847584722828 0.005610783265343842 38-39 0.0 0.0 0.0 0.1914267231705546 0.005940829339775832 40-41 0.0 0.0 0.0 0.24324395685637712 0.006930967563071805 42-43 0.0 0.0 0.0 0.28119925541605606 0.007261013637503796 44-45 0.0 0.0 0.0 0.30727289529618335 0.007261013637503796 46-47 0.0 0.0 0.0 0.35050893104677416 0.007261013637503796 48-49 0.0 0.0 0.0 0.38384358456440515 0.007921105786367778 50-51 0.0 0.0 0.0 0.40463648725362056 0.007921105786367778 52-53 0.0 0.0 0.0 0.4554635827161472 0.008581197935231759 54-55 0.0 0.0 0.0 0.5455661610360807 0.00924129008409574 56-57 0.0 0.0 0.0 0.6673531625014852 0.00924129008409574 58-59 0.0 0.0 0.0 0.78418947285041 0.00924129008409574 60-61 0.0 0.0 0.0 0.9036661517947906 0.00924129008409574 62-63 0.0 0.0 0.0 0.9970691908590439 0.010561474381823702 64-65 0.0 0.0 0.0 1.102353888602849 0.010561474381823702 66-67 0.0 0.0 0.0 1.27991867664726 0.010561474381823702 68-69 0.0 0.0 0.0 1.5000594082933978 0.010561474381823702 70-71 0.0 0.0 0.0 1.7584854845736464 0.011551612605119674 72-73 0.0 0.0 0.0 2.034404002798791 0.014522027275007591 74-75 0.0 0.0 0.0 2.3314454697875826 0.014522027275007591 76-77 0.0 0.0 0.0 2.6040635272684067 0.014522027275007591 78-79 0.0 0.0 0.0 2.9017650864060625 0.014522027275007591 80-81 0.0 0.0 0.0 3.185604710417574 0.015182119423871574 82-83 0.0 0.0 0.0 3.5262122592313885 0.015842211572735555 84-85 0.0 0.0 0.0 4.042404319643023 0.015842211572735555 86-87 0.0 0.0 0.0 4.626255825313214 0.015842211572735555 88-89 0.0 0.0 0.0 5.297239494633451 0.015842211572735555 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTTCT 25 0.001613767 38.000793 12-13 GTAAGCG 25 0.001615079 37.994507 94-95 TAAGGCG 35 2.0535027E-4 33.91806 46-47 CAGGGAG 60 0.003914325 31.709284 5 TTTGGAC 65 0.0058088144 29.250732 3 AGGGCGT 105 6.8659145E-5 27.143423 8 AATGCTA 35 0.008387874 27.138933 18-19 GAGTACT 985 0.0 25.55891 12-13 GTACTTT 1005 0.0 24.813953 14-15 TGGACAG 100 0.0016341791 23.781963 5 TGCACCG 100 0.0016341791 23.781963 5 TACCCCA 105 0.0021704265 22.649488 5 AAGACTT 105 0.0021704265 22.649488 5 CTTGAAG 105 0.002178803 22.634495 3 CACCGTC 105 0.0021872062 22.619518 7 ACTTTTT 1130 0.0 22.279226 16-17 ACATAAT 65 2.9341574E-4 21.923536 78-79 GCACCGT 110 0.0028655964 21.59136 6 TGGGGAG 110 0.0028655964 21.59136 6 ACCCCAT 110 0.0028655964 21.59136 6 >>END_MODULE