FastQCFastQC Report
Thu 26 May 2016
SRR937811_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937811_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151494
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21101.3927944341030007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14210.9379909435357177No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13590.8970652303061507No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13500.8911244009663749No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10580.6983774934980923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9180.6059645926571349No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT9040.5967233025730392No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC6570.4336805418036358No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG5990.3953951971695248No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC5830.38483372278770117No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA5810.38351353848997316No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4770.31486395500811915No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC4520.2983616512865196No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT4400.29044054550015186No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT4290.28317953186264805No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC4010.26469695169445656No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4000.2640368595455926No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG3860.25479556946149684No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA3590.23697308144216933No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC3270.21585013267852193No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3260.21519004052965793No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA3200.21122948763647406No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG3070.20264828970124232No Hit
GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG2920.19274690746828257No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT2860.18878635457509868No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC2860.18878635457509868No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG2610.17228405085349915No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC2510.16568312936485935No Hit
GATAATACGTCTCAATGAAGTCACATAAGTGGGGATCATTCTTGTCAGTA2400.15842211572735554No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2080.13729916696370814No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.1346587983682522No Hit
CCCTGGGACACGGTGATGAGAGCTAAGCTGACTTCCCCAAAGCCACGTGA2020.13333861407052425No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG2000.1320184297727963No Hit
GGTGAAATCCATTAAAGAACTGGGTGACCACGTGACCAACTTACGCAAGA1960.12937806117734035No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC1870.12343723183756451No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG1870.12343723183756451No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC1860.12277713968870053No Hit
TCCTACGTCTATCTGTCTATGTCTTGTTATTTTGACCGAGATGATGTGGC1760.11617621820006073No Hit
AGATGATGTGGCTCTGAAGAACTTTGCCAAATACTTTCTCCACCAATCTC1710.11287575745574081No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT1700.11221566530687684No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC1690.11155557315801286No Hit
CTCATGAGGAGAGGGAGCATGCCGAGAAACTGATGAAGCTGCAGAACCAG1630.10759502026482896No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTAG500.001608085838.0158581
TGCTAGC500.001608085838.0158582
CTAGCCA550.002567326234.5598684
GCTAGCC650.005816391729.2429663
TACAGAG650.005816391729.2429662
GCGCTTG650.005835206729.2236589
CATGGGC1004.9111404E-528.5118944
GTCCAAG1554.514004E-827.5921541
GAGTACT11600.024.5629912-13
TGCGACT601.7095849E-423.74422352-53
GTACTTT12200.023.16034914-15
CGACTGG652.939422E-421.91774454-55
GATCCAT550.002862833221.60348742-43
CAGGAGC1100.002859032521.5999184
GACTGGT1100.0028700221.5856576
ACTTTTT13100.021.56917816-17
TGCAGAC1553.86521E-521.4605642
AGTACTT11350.021.3384212-13
GAAGCGA704.8375584E-420.35891282-83
CATGGGG4300.019.892024
Produced by FastQC (version 0.11.5)