##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937811_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 151494 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.012693572022656 31.0 31.0 34.0 30.0 34.0 2 32.28829524601635 33.0 31.0 34.0 31.0 34.0 3 32.34515558371949 34.0 31.0 34.0 30.0 34.0 4 36.02232431647458 37.0 35.0 37.0 35.0 37.0 5 35.91747527954902 37.0 35.0 37.0 35.0 37.0 6 35.990719104386976 37.0 35.0 37.0 35.0 37.0 7 35.927040014786066 37.0 35.0 37.0 35.0 37.0 8 35.916544549619125 37.0 35.0 37.0 35.0 37.0 9 37.570794882965664 39.0 37.0 39.0 35.0 39.0 10-11 37.48810183901672 39.0 37.0 39.0 35.0 39.0 12-13 37.38250029704147 39.0 37.0 39.0 34.0 39.0 14-15 38.49172904537474 40.0 38.0 41.0 34.0 41.0 16-17 38.44807385110961 40.0 38.0 41.0 34.0 41.0 18-19 38.43677637398181 40.0 38.0 41.0 34.0 41.0 20-21 38.3403237091898 40.0 38.0 41.0 34.0 41.0 22-23 38.26424808903323 40.0 38.0 41.0 34.0 41.0 24-25 38.209509947588685 40.0 37.5 41.0 33.5 41.0 26-27 37.99135609331063 40.0 37.0 41.0 33.0 41.0 28-29 37.79156600261396 40.0 37.0 41.0 33.0 41.0 30-31 37.78984316210543 40.0 37.0 41.0 33.0 41.0 32-33 37.60961490224035 40.0 36.0 41.0 33.0 41.0 34-35 37.371272129589286 40.0 35.0 41.0 33.0 41.0 36-37 37.1518574993069 39.0 35.0 41.0 32.5 41.0 38-39 36.94337729547045 39.0 35.0 41.0 32.0 41.0 40-41 36.71969186898491 39.0 35.0 41.0 31.0 41.0 42-43 36.38140124361361 39.0 35.0 41.0 31.0 41.0 44-45 35.94232774895376 39.0 35.0 40.5 30.0 41.0 46-47 35.36006706536233 38.0 35.0 40.0 28.0 41.0 48-49 34.81329623615457 38.0 34.5 40.0 26.0 41.0 50-51 34.392675617516204 38.0 34.0 40.0 24.0 41.0 52-53 34.09776954862899 38.0 34.0 40.0 23.0 41.0 54-55 33.777829484996104 37.0 33.5 40.0 22.0 41.0 56-57 33.36822250386154 37.0 33.0 40.0 20.5 41.0 58-59 33.054285978322575 36.0 33.0 40.0 19.0 41.0 60-61 32.58677571388966 36.0 32.5 39.0 16.0 41.0 62-63 32.16920472097905 35.0 32.0 39.0 12.0 40.0 64-65 31.744105377110643 35.0 31.0 38.5 7.5 40.0 66-67 31.23270558569976 35.0 31.0 38.0 3.5 40.0 68-69 30.72715091026707 35.0 30.5 37.0 2.0 39.5 70-71 30.25945251957173 35.0 30.0 36.5 2.0 39.0 72-73 29.768310956209486 34.0 29.5 36.0 2.0 39.0 74-75 29.320537447027604 34.0 29.0 35.5 2.0 37.5 76-77 28.145919970427872 32.5 28.0 35.0 2.0 36.0 78-79 28.69211981992686 34.0 29.0 35.0 2.0 36.0 80-81 28.51776307972593 34.0 29.0 35.0 2.0 36.0 82-83 28.171135490514477 34.0 28.5 35.0 2.0 36.0 84-85 27.833617172957346 33.5 27.0 35.0 2.0 35.0 86-87 27.522245105416715 33.0 27.0 35.0 2.0 35.0 88-89 27.09193433403303 33.0 26.0 35.0 2.0 35.0 90-91 26.563669188218675 33.0 25.0 35.0 2.0 35.0 92-93 26.17400359090129 33.0 24.0 35.0 2.0 35.0 94-95 25.70683327392504 32.5 23.5 34.5 2.0 35.0 96-97 25.073854410075647 32.0 19.0 34.0 2.0 35.0 98-99 24.23373532945199 32.0 6.0 34.0 2.0 35.0 100-101 21.714774182475875 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2912605359058702 1101 2 0.16815788166018564 1101 3 -0.2844417930147287 1101 4 -0.030613239924313973 1101 5 -0.0752848126515886 1101 6 -0.15558691921357592 1101 7 -0.09937551753263563 1101 8 -0.175233697395214 1101 9 -0.16496539790470877 1101 10-11 0.0019811750284119967 1101 12-13 0.07127757466975737 1101 14-15 0.18398825732847257 1101 16-17 -0.09729040350130447 1101 18-19 -0.16558106229263103 1101 20-21 0.04676468374985632 1101 22-23 0.0799296388354449 1101 24-25 -0.08330675204502569 1101 26-27 0.08296614248151002 1101 28-29 0.16203525952101927 1101 30-31 -0.16370351507887904 1101 32-33 0.15187031617642077 1101 34-35 -0.30402167272243474 1101 36-37 0.21577340054741967 1101 38-39 0.38843738021665786 1101 40-41 0.29851249805477664 1101 42-43 0.6352610988104033 1101 44-45 0.8644881089203551 1101 46-47 0.7581230581888789 1101 48-49 0.3483340615219461 1101 50-51 0.30300249818989045 1101 52-53 0.24778822474062423 1101 54-55 0.05098306967065014 1101 56-57 0.09686236506492918 1101 58-59 -0.4995077870064293 1101 60-61 0.21609337073638812 1101 62-63 -0.2699668401208797 1101 64-65 0.013399400558562036 1101 66-67 -0.5691500736494781 1101 68-69 -0.6837864519149726 1101 70-71 -0.6427188139236435 1101 72-73 -1.2138364394789 1101 74-75 -1.1927922963835726 1101 76-77 -1.3689147068853416 1101 78-79 -1.2494387918259733 1101 80-81 -1.1997665633760803 1101 82-83 -1.0251157936604294 1101 84-85 -0.621767416906998 1101 86-87 -0.8203308496705972 1101 88-89 -0.8150429386986495 1101 90-91 -0.9602503474391497 1101 92-93 -0.328374888373105 1101 94-95 -0.6004705886578563 1101 96-97 -1.1990199736610698 1101 98-99 -0.8772249135232002 1101 100-101 -1.1526220840048076 1102 1 0.4393916120037602 1102 2 0.4721722966786217 1102 3 0.4130523404080009 1102 4 0.20421746741728697 1102 5 -0.03903774594022735 1102 6 -0.0866546362769327 1102 7 -0.08139864860873303 1102 8 0.0739282201374678 1102 9 0.45156996917540937 1102 10-11 0.08979126554131511 1102 12-13 0.31968521798185634 1102 14-15 0.3708177913546251 1102 16-17 0.588219150638821 1102 18-19 0.4005855485018728 1102 20-21 0.648528847343556 1102 22-23 0.19015843534901933 1102 24-25 0.1719731674990541 1102 26-27 0.06389976635009731 1102 28-29 0.13337283913885756 1102 30-31 -0.01638956938662517 1102 32-33 -0.12878255006561545 1102 34-35 0.15357135443143477 1102 36-37 0.11287782562285287 1102 38-39 0.3121718756910141 1102 40-41 0.5216938591007079 1102 42-43 0.5077049472850916 1102 44-45 -0.2972408552097079 1102 46-47 -0.09284492706087377 1102 48-49 -0.9834208831646123 1102 50-51 -0.7888094361044438 1102 52-53 -0.2971539475691287 1102 54-55 -0.21328528433539162 1102 56-57 -0.40246716896121626 1102 58-59 -0.18585542361387297 1102 60-61 0.4874854257145955 1102 62-63 0.20101455444834926 1102 64-65 -0.3429532645436879 1102 66-67 -0.8335022359022446 1102 68-69 -0.43089506740273364 1102 70-71 -0.009966551100983878 1102 72-73 -0.2648547096766869 1102 74-75 0.3890045524263499 1102 76-77 0.6608162686426517 1102 78-79 0.43387079583745347 1102 80-81 -0.08488640916890589 1102 82-83 0.40882235585348425 1102 84-85 0.6703671791372514 1102 86-87 0.5647889961222283 1102 88-89 0.8472936352202218 1102 90-91 0.9019060387492495 1102 92-93 0.8426777432058437 1102 94-95 1.24618127188522 1102 96-97 1.286271679037558 1102 98-99 1.1949968430976519 1102 100-101 2.0198762398302605 1103 1 0.37394887439916147 1103 2 0.40702097075546817 1103 3 0.26393281998836926 1103 4 0.17836508824905906 1103 5 -0.028630185888602 1103 6 0.2180370917943364 1103 7 0.24156875491318885 1103 8 0.21184920594156154 1103 9 -0.15477447943205647 1103 10-11 0.256603638048702 1103 12-13 0.26082005832989097 1103 14-15 0.4896513120240371 1103 16-17 0.4982978317928115 1103 18-19 0.0872139153475473 1103 20-21 0.27941432255275345 1103 22-23 0.3947086206035877 1103 24-25 -0.06490289927585735 1103 26-27 0.04964140907937775 1103 28-29 -0.09286670126329 1103 30-31 0.1554184319455416 1103 32-33 -0.17043360539220487 1103 34-35 -0.4094568292411864 1103 36-37 0.16393731523611166 1103 38-39 -0.04878312201932289 1103 40-41 -0.05829864745605562 1103 42-43 -0.32947918326733827 1103 44-45 0.15940124961523594 1103 46-47 0.06060413834023137 1103 48-49 0.19005232777581682 1103 50-51 -0.074663480482279 1103 52-53 0.6143817919920807 1103 54-55 0.6300809368877083 1103 56-57 0.8381764345523735 1103 58-59 -4.967790944974126E-4 1103 60-61 0.053490171561982436 1103 62-63 0.09009077941816912 1103 64-65 0.1422263699428079 1103 66-67 0.14195673749603444 1103 68-69 0.23085923091956673 1103 70-71 0.14727086615891594 1103 72-73 0.014255235787697274 1103 74-75 -0.15443660322911867 1103 76-77 -0.1288261275939746 1103 78-79 -0.05299486337739978 1103 80-81 -0.07096109381983595 1103 82-83 0.01949634944242362 1103 84-85 0.033611840058945575 1103 86-87 -0.07331932576278888 1103 88-89 -0.19937386405124968 1103 90-91 0.06505495011846918 1103 92-93 0.18254702425159408 1103 94-95 -0.29403354015424554 1103 96-97 0.1417523001607428 1103 98-99 -0.45782776870035846 1103 100-101 -0.10349497702166133 1104 1 0.3466859144206538 1104 2 0.27585434885556737 1104 3 0.38399728413018863 1104 4 0.13485179251981094 1104 5 0.17931143824963414 1104 6 0.36505114855859233 1104 7 0.3459200844861101 1104 8 0.15803955422698834 1104 9 0.1819163612877901 1104 10-11 0.33593034590079895 1104 12-13 0.13634606782044045 1104 14-15 0.5702986394481613 1104 16-17 0.6133260348548575 1104 18-19 -0.08041523676991602 1104 20-21 0.3442142151125154 1104 22-23 0.22056590415628108 1104 24-25 0.4174142284884965 1104 26-27 0.4226380963387584 1104 28-29 0.5181996430826672 1104 30-31 0.39483109198673105 1104 32-33 0.5174525079572376 1104 34-35 0.5849473251146193 1104 36-37 1.0211537177788585 1104 38-39 0.5450704011250949 1104 40-41 0.5522976458557878 1104 42-43 0.6704088998197761 1104 44-45 0.8389383612635442 1104 46-47 0.9315639377851284 1104 48-49 1.2442424969941897 1104 50-51 1.222162555868337 1104 52-53 1.514686205995126 1104 54-55 1.4098060689496634 1104 56-57 1.2884629418493532 1104 58-59 1.2395873824239985 1104 60-61 1.902872390202866 1104 62-63 1.5551503192158194 1104 64-65 1.8506380451151614 1104 66-67 1.8569043603808062 1104 68-69 2.1748112409473244 1104 70-71 2.1126885400777944 1104 72-73 2.0848748078174246 1104 74-75 1.6859027288507455 1104 76-77 0.7961447740086776 1104 78-79 1.217441164915197 1104 80-81 1.0382921965374017 1104 82-83 1.6806790873777828 1104 84-85 1.9526287618961717 1104 86-87 1.6676377876761954 1104 88-89 1.2898597289293257 1104 90-91 1.8361177131232118 1104 92-93 1.8871982911510479 1104 94-95 2.3254937228057315 1104 96-97 2.05215161970591 1104 98-99 2.2631409840975643 1104 100-101 2.096701996745015 1105 1 0.20686203966526762 1105 2 0.21421051323913076 1105 3 -0.01884028925532988 1105 4 0.00735668488771779 1105 5 0.19858736779954 1105 6 0.1588867649969501 1105 7 0.2150981666778904 1105 8 -0.015835201154615675 1105 9 0.058530842696789875 1105 10-11 0.2735037314193889 1105 12-13 -0.38586528247309815 1105 14-15 0.38972134003524417 1105 16-17 0.1777095964986941 1105 18-19 -0.1915209119166903 1105 20-21 -0.27926915083660475 1105 22-23 -0.18745757920967066 1105 24-25 -0.15323156407510652 1105 26-27 0.10571050338376864 1105 28-29 0.1298924023781609 1105 30-31 0.3083829510865357 1105 32-33 0.36437031617641935 1105 34-35 -0.0748863343765791 1105 36-37 0.1455666336301249 1105 38-39 -0.32415660474574537 1105 40-41 0.1635500920397419 1105 42-43 0.4728550837728065 1105 44-45 0.029055778093294293 1105 46-47 -0.15155739293894754 1105 48-49 0.14551451264976833 1105 50-51 -0.16203509579506914 1105 52-53 -0.06339598578568939 1105 54-55 -0.25061467469025445 1105 56-57 0.4643435680724437 1105 58-59 -0.03296643362296692 1105 60-61 -0.15862467437639083 1105 62-63 -0.21329390779005308 1105 64-65 0.23821143063375416 1105 66-67 -0.12695082552917825 1105 68-69 -0.028711263945044863 1105 70-71 -0.19215490414920922 1105 72-73 0.10204701916771697 1105 74-75 -0.22794267232342236 1105 76-77 -0.20679064673496228 1105 78-79 -0.10780345348010911 1105 80-81 -0.11395829269939028 1105 82-83 0.021970672504984634 1105 84-85 0.05125889888247315 1105 86-87 -0.1347105489187186 1105 88-89 -0.23196023193173332 1105 90-91 0.11503160744806706 1105 92-93 -0.15732225679415635 1105 94-95 0.13796926096620155 1105 96-97 -0.278719221781369 1105 98-99 0.35125253008581936 1105 100-101 0.9316824272733903 1106 1 0.20150489680812456 1106 2 0.25885337038198486 1106 3 0.30437399645895624 1106 4 0.30914239917343167 1106 5 0.2057302249423998 1106 6 0.10710105071123621 1106 7 -0.2206161190363929 1106 8 0.04845051313110105 1106 9 0.25138798555393294 1106 10-11 0.18689658856224156 1106 12-13 -0.22782956818738143 1106 14-15 0.15132848289238865 1106 16-17 -0.04818326064416567 1106 18-19 0.07901480236902358 1106 20-21 0.37430227773482727 1106 22-23 0.6250424207903293 1106 24-25 0.5449827216391796 1106 26-27 0.5244605033837644 1106 28-29 0.5459638309495887 1106 30-31 0.5324900939436716 1106 32-33 0.3036560304621361 1106 34-35 -0.26149347723371363 1106 36-37 0.5169952050587057 1106 38-39 0.9803076809685365 1106 40-41 0.6706929491826017 1106 42-43 -0.12625205908433657 1106 44-45 -0.330765650478142 1106 46-47 0.774335464203908 1106 48-49 0.34997879836404877 1106 50-51 0.3638577613477878 1106 52-53 0.10892544278573979 1106 54-55 -0.08365038897596833 1106 56-57 -1.1294064319275563 1106 58-59 -0.5302878621943989 1106 60-61 -0.5773746743763937 1106 62-63 -0.6007939077900524 1106 64-65 -0.9055385693662501 1106 66-67 -1.1269508255291782 1106 68-69 -0.5108541210879061 1106 70-71 -1.0689406184349224 1106 72-73 -0.6193815522608546 1106 74-75 -0.7216926723234245 1106 76-77 -0.6532192181635352 1106 78-79 -0.09976773919439452 1106 80-81 -0.7916368641279625 1106 82-83 -0.4762436132093022 1106 84-85 -1.0987411011175254 1106 86-87 -0.7686391203472915 1106 88-89 -0.34088880336030414 1106 90-91 -0.4322898211233621 1106 92-93 -0.9698222567941563 1106 94-95 -1.438816453319511 1106 96-97 -0.6858620789242273 1106 98-99 0.43339538722867843 1106 100-101 -0.5334961441551833 1107 1 0.438700356387308 1107 2 0.2723500481228527 1107 3 0.26028484917213746 1107 4 0.3151639938578157 1107 5 0.3496947875116092 1107 6 0.1561735867023799 1107 7 0.5069586317941699 1107 8 0.2034892728210238 1107 9 0.5725397020544918 1107 10-11 0.5057296450406525 1107 12-13 0.2689519932744062 1107 14-15 0.3922960908658055 1107 16-17 0.23701192208009303 1107 18-19 0.2407326871973794 1107 20-21 0.5026245368710391 1107 22-23 0.26436966331525014 1107 24-25 0.10069977590274704 1107 26-27 0.01916565288542671 1107 28-29 0.18141510448225517 1107 30-31 -0.008588256000948036 1107 32-33 -0.3079165055290005 1107 34-35 -0.45110449605984826 1107 36-37 -0.23065152805314426 1107 38-39 -0.7039738804932512 1107 40-41 -0.9370036178162877 1107 42-43 -0.501009811022314 1107 44-45 -0.4517858608878811 1107 46-47 -0.2529693530718333 1107 48-49 -0.9734223644266464 1107 50-51 -0.7279542541560886 1107 52-53 0.1660613785553906 1107 54-55 0.11419706395869156 1107 56-57 -0.16037957699953864 1107 58-59 0.019719059178804343 1107 60-61 -0.4528937773664339 1107 62-63 -0.5532440739030093 1107 64-65 -0.36909753946591906 1107 66-67 -0.09151339474290765 1107 68-69 -0.25850085419975244 1107 70-71 -0.6657706295091188 1107 72-73 -0.8700736895809023 1107 74-75 -0.8631309336744728 1107 76-77 -0.6508659512753852 1107 78-79 -0.8059208399695876 1107 80-81 -0.8336149925886467 1107 82-83 -0.8351168136522702 1107 84-85 -0.4340124189469847 1107 86-87 -0.11283900960532023 1107 88-89 -0.06728692074679188 1107 90-91 -0.13892741802258968 1107 92-93 -0.21933776067012545 1107 94-95 -0.6070750358222803 1107 96-97 -0.23528068357538956 1107 98-99 -0.058298964930788344 1107 100-101 -0.2834615372891811 1108 1 0.16132632537955516 1108 2 -0.07485198676086924 1108 3 -0.09406350354104376 1108 4 -0.03349152939799893 1108 5 0.07068558208525388 1108 6 -0.014550735003048487 1108 7 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-0.12394832535981237 2316 48-49 -0.3987113213964335 2316 50-51 -0.26731738835280794 2316 52-53 -0.10950377021682556 2316 54-55 0.1918789095697946 2316 56-57 0.04785511640435658 2316 58-59 0.11564348938815527 2316 60-61 0.07732485513600551 2316 62-63 -0.028456439868747907 2316 64-65 -0.1802659004184335 2316 66-67 -0.26381994443422485 2316 68-69 0.06538625530217601 2316 70-71 -0.3880702163818839 2316 72-73 -0.17209754883912964 2316 74-75 -0.48904190243462864 2316 76-77 0.42451816421456456 2316 78-79 -0.1007033679369087 2316 80-81 0.10028464998666564 2316 82-83 -0.20843993485172874 2316 84-85 -0.5074237358480644 2316 86-87 -0.7640946378836482 2316 88-89 -1.0787523619573953 2316 90-91 -0.5196775456742628 2316 92-93 0.0163304378166238 2316 94-95 -0.2934464789824709 2316 96-97 0.037940316285354214 2316 98-99 0.2246699808984829 2316 100-101 -0.0403663323502208 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 11.0 6 21.0 7 33.0 8 91.0 9 155.0 10 271.0 11 355.0 12 481.0 13 735.0 14 996.0 15 1368.0 16 1756.0 17 2492.0 18 2275.0 19 1489.0 20 1308.0 21 1488.0 22 1302.0 23 1376.0 24 1566.0 25 1704.0 26 1892.0 27 2211.0 28 2745.0 29 3395.0 30 4111.0 31 5008.0 32 6198.0 33 8147.0 34 11169.0 35 16941.0 36 24030.0 37 28414.0 38 15351.0 39 607.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.62020928927475 19.166804212194236 15.347440002640875 20.86554649589014 2 17.46933871968527 22.396266518806026 34.76045255917726 25.373942202331445 3 21.30117364384068 23.585092478910056 30.65335920894557 24.460374668303693 4 14.245448664633583 17.965067923482117 34.359776624816824 33.429706787067474 5 14.284393674271186 36.79685694476543 32.42431245666744 16.49443692429595 6 31.494316606598282 34.80665900959774 15.483781535902413 18.215242847901568 7 27.38392279562227 34.253501788849725 19.39944816296355 18.96312725256446 8 26.907996356291335 30.496917369664807 19.306375169973727 23.288711104070128 9 25.212879718008637 18.438353994217593 20.65098287720966 35.697783410564114 10-11 26.48157682812521 25.403646349030325 24.556748122037835 23.55802870080663 12-13 27.824224281051034 23.288896516897324 26.100661106288598 22.786218095763044 14-15 24.1890767951206 25.782869288552686 26.624816824428688 23.40323709189803 16-17 22.013083026390483 28.356898623047776 27.065098287720968 22.564920062840773 18-19 21.219982309530412 26.893804375090763 29.985015908220785 21.90119740715804 20-21 23.290361334442288 24.577871070801486 29.55001518211942 22.581752412636806 22-23 22.315882582033495 24.509053223581287 30.402724878377217 22.772339316008 24-25 23.828996527915297 25.420478698826354 29.736821260247932 21.013703513010416 26-27 22.872975952366808 24.632486418335084 31.519364186651348 20.975173442646756 28-29 20.989817977787677 26.9379672261003 30.629899169925906 21.442315626186115 30-31 21.96093574663023 25.2785588868206 30.19756558015499 22.562939786394182 32-33 22.350390114459977 25.717190119740717 30.418366403949992 21.514053361849314 34-35 23.26890833960421 25.345888286004726 29.41139583085799 21.973807543533077 36-37 22.88737507756083 24.75972645781351 30.697915428993888 21.654983035631776 38-39 22.260617582214476 25.517842290783793 29.996237474751474 22.225302652250253 40-41 21.927560531249174 25.71785968896047 30.0187468645209 22.335832915269453 42-43 22.476308675431326 26.151723500539674 28.27148227978043 23.10048554424856 44-45 22.56128955602202 25.291430683723448 28.66582174871612 23.48145801153841 46-47 23.634599390074854 26.655511109350865 28.07074867651524 21.639140824059037 48-49 23.84648896986019 25.61718615918782 28.214318718893157 22.322006152058826 50-51 22.255006798949132 26.666402629807124 28.586610690852442 22.491979880391302 52-53 23.482778195836136 27.400755145418298 26.174303932829023 22.94216272591654 54-55 22.294942373955404 26.44032106882121 26.96806474183796 24.29667181538543 56-57 23.498620407408875 26.49510871717692 26.644619588894606 23.361651286519596 58-59 24.366971629239444 25.674614176138988 26.991167967048202 22.96724622757337 60-61 23.15834290466949 26.54263535189512 28.815992712582677 21.483029030852705 62-63 21.927271047038165 26.646929911415633 28.3519479319313 23.0738511096149 64-65 21.86456229289609 27.54168481920076 27.55422657002918 23.039526317873975 66-67 22.702879321953347 26.471015353743383 26.733071936842386 24.09303338746089 68-69 22.176785879308753 26.113575455133535 27.49184786196153 24.21779080359618 70-71 23.69301754524932 26.727461153577035 26.604353967813903 22.975167333359735 72-73 23.453404095211692 25.560088188311088 27.867110248590706 23.119397467886518 74-75 22.463925964064583 28.024872272169194 26.04591600987498 23.465285753891244 76-77 22.57911204404135 26.50203968473999 26.499729362218964 24.419118908999696 78-79 22.789021347380096 26.461444017584856 26.92845921290612 23.821075422128928 80-81 23.256696634850226 27.009980593290823 26.335696463226267 23.397626308632685 82-83 22.816641087842633 26.246843903163526 27.157780088123175 23.77873492087067 84-85 22.659973332277186 26.61788585686562 26.500059408293396 24.2220814025638 86-87 22.74743554200166 26.132388081376163 26.557817471318995 24.56235890530318 88-89 22.305173802262797 26.388833881209816 25.762076385863462 25.543915930663918 90-91 22.85388919146408 25.536135729728837 26.402931128385404 25.20704395042168 92-93 23.039830486098275 25.645908694848572 26.86544504000211 24.44881577905104 94-95 22.965265951126774 25.47295602466104 26.840666957107214 24.721111067104967 96-97 21.77809022139491 26.125127067738656 27.410986573725694 24.685796137140745 98-99 22.73090328202149 26.410305494679587 25.395400417183744 25.46339080611518 100-101 22.27221411641986 25.39034389875156 26.350606394707825 25.986835590120748 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 722.0 1 471.0 2 238.0 3 258.0 4 208.0 5 187.0 6 306.5 7 494.5 8 605.0 9 633.5 10 569.0 11 434.0 12 399.0 13 448.5 14 466.5 15 453.5 16 472.0 17 497.5 18 516.5 19 506.5 20 490.0 21 526.0 22 576.5 23 638.5 24 664.5 25 647.5 26 672.0 27 773.0 28 859.0 29 908.0 30 1021.0 31 1118.0 32 1168.0 33 1297.5 34 1516.5 35 1575.5 36 1584.0 37 1741.5 38 2009.5 39 2225.0 40 2332.0 41 2890.0 42 3589.5 43 3987.0 44 4079.0 45 4165.5 46 4450.0 47 6068.0 48 9578.0 49 9709.0 50 6887.5 51 5664.5 52 5445.5 53 5444.0 54 5506.5 55 5285.0 56 4571.0 57 4025.0 58 3898.0 59 3733.5 60 3444.0 61 3111.0 62 2668.5 63 2249.0 64 1792.0 65 1386.0 66 1060.5 67 956.0 68 750.0 69 435.5 70 378.0 71 338.5 72 318.0 73 248.5 74 162.5 75 138.5 76 84.5 77 51.0 78 37.0 79 21.0 80 9.0 81 3.5 82 3.5 83 2.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01914267231705546 2 0.0 3 0.0 4 0.0 5 0.03234451529433509 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003630506818751898 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.600921488639814E-4 24-25 0.0 26-27 0.0019802764465919444 28-29 9.901382232959722E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0013201842977279628 42-43 0.00957133615852773 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 9.901382232959722E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.010891520456255693 92-93 0.0013201842977279628 94-95 0.0 96-97 0.0 98-99 0.0013201842977279628 100-101 0.016502303721599536 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 151494.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.93891507254413 #Duplication Level Percentage of deduplicated Percentage of total 1 71.03837471783295 29.082339894649294 2 13.971299580780395 11.439396939812797 3 5.727184779103514 7.033941938294586 4 2.6765559496936473 4.383011868456837 5 1.6156078684295387 3.307061665808547 6 1.0174137375040309 2.499108875599034 7 0.6465656239922606 1.8528786618611959 8 0.48210254756530146 1.5789404200826436 9 0.3708481135117704 1.3663907481484416 >10 2.142857142857143 16.48316104928248 >50 0.20799742018703643 5.853037083976923 >100 0.0854563044179297 7.503267456136877 >500 0.009674298613350531 2.8001108954810094 >1k 0.008061915511125443 4.817352502409336 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2110 1.3927944341030007 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1421 0.9379909435357177 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1359 0.8970652303061507 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1350 0.8911244009663749 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1058 0.6983774934980923 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 918 0.6059645926571349 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 904 0.5967233025730392 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 657 0.4336805418036358 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 599 0.3953951971695248 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 583 0.38483372278770117 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 581 0.38351353848997316 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 477 0.31486395500811915 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 452 0.2983616512865196 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 440 0.29044054550015186 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 429 0.28317953186264805 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 401 0.26469695169445656 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 400 0.2640368595455926 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 386 0.25479556946149684 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 359 0.23697308144216933 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 327 0.21585013267852193 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 326 0.21519004052965793 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 320 0.21122948763647406 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 307 0.20264828970124232 No Hit GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG 292 0.19274690746828257 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 286 0.18878635457509868 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 286 0.18878635457509868 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 261 0.17228405085349915 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 251 0.16568312936485935 No Hit GATAATACGTCTCAATGAAGTCACATAAGTGGGGATCATTCTTGTCAGTA 240 0.15842211572735554 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 208 0.13729916696370814 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 204 0.1346587983682522 No Hit CCCTGGGACACGGTGATGAGAGCTAAGCTGACTTCCCCAAAGCCACGTGA 202 0.13333861407052425 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 200 0.1320184297727963 No Hit GGTGAAATCCATTAAAGAACTGGGTGACCACGTGACCAACTTACGCAAGA 196 0.12937806117734035 No Hit GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC 187 0.12343723183756451 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 187 0.12343723183756451 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 186 0.12277713968870053 No Hit TCCTACGTCTATCTGTCTATGTCTTGTTATTTTGACCGAGATGATGTGGC 176 0.11617621820006073 No Hit AGATGATGTGGCTCTGAAGAACTTTGCCAAATACTTTCTCCACCAATCTC 171 0.11287575745574081 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 170 0.11221566530687684 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 169 0.11155557315801286 No Hit CTCATGAGGAGAGGGAGCATGCCGAGAAACTGATGAAGCTGCAGAACCAG 163 0.10759502026482896 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0013201842977279628 2 0.0 0.0 0.0 0.0 0.0013201842977279628 3 0.0 0.0 0.0 0.0 0.0013201842977279628 4 0.0 0.0 0.0 0.0 0.0013201842977279628 5 0.0 0.0 0.0 0.0 0.0013201842977279628 6 0.0 0.0 0.0 0.0 0.0019802764465919444 7 0.0 0.0 0.0 0.0 0.0019802764465919444 8 0.0 0.0 0.0 0.0 0.0026403685954559256 9 0.0 0.0 0.0 0.0 0.003300460744319907 10-11 0.0 0.0 0.0 0.0 0.003300460744319907 12-13 0.0 0.0 0.0 0.0 0.003300460744319907 14-15 0.0 0.0 0.0 0.0 0.0036305068187518982 16-17 0.0 0.0 0.0 0.0 0.004290598967615879 18-19 0.0 0.0 0.0 0.0 0.00462064504204787 20-21 0.0 0.0 0.0 0.0 0.005280737190911851 22-23 0.0 0.0 0.0 0.0019802764465919444 0.005940829339775832 24-25 0.0 0.0 0.0 0.00462064504204787 0.006600921488639814 26-27 0.0 0.0 0.0 0.008581197935231759 0.007921105786367778 28-29 0.0 0.0 0.0 0.023103225210239352 0.007921105786367778 30-31 0.0 0.0 0.0 0.04092571322956685 0.007921105786367778 32-33 0.0 0.0 0.0 0.06303880021651023 0.00924129008409574 34-35 0.0 0.0 0.0 0.09373308513868536 0.011881658679551665 36-37 0.0 0.0 0.0 0.1353188905171162 0.012211704753983657 38-39 0.0 0.0 0.0 0.19340699961714655 0.012541750828415648 40-41 0.0 0.0 0.0 0.24489418722853712 0.012541750828415648 42-43 0.0 0.0 0.0 0.282849485788216 0.012871796902847638 44-45 0.0 0.0 0.0 0.3099132638916393 0.013201842977279629 46-47 0.0 0.0 0.0 0.35446948393995803 0.01386193512614361 48-49 0.0 0.0 0.0 0.3878041374575891 0.01386193512614361 50-51 0.0 0.0 0.0 0.40793694799794056 0.01386193512614361 52-53 0.0 0.0 0.0 0.461404412055923 0.014852073349439582 54-55 0.0 0.0 0.0 0.5574478197156323 0.016172257647167544 56-57 0.0 0.0 0.0 0.6805550054787648 0.016502303721599536 58-59 0.0 0.0 0.0 0.8000316844231454 0.016502303721599536 60-61 0.0 0.0 0.0 0.919838409441958 0.017162395870463518 62-63 0.0 0.0 0.0 1.0148916788783713 0.0178224880193275 64-65 0.0 0.0 0.0 1.1201763766221764 0.0178224880193275 66-67 0.0 0.0 0.0 1.2987313028898835 0.0178224880193275 68-69 0.0 0.0 0.0 1.521512403131477 0.0178224880193275 70-71 0.0 0.0 0.0 1.7845591244537737 0.0178224880193275 72-73 0.0 0.0 0.0 2.06080768875335 0.020462856614783424 74-75 0.0 0.0 0.0 2.3614796625608934 0.020462856614783424 76-77 0.0 0.0 0.0 2.6357479504138777 0.020462856614783424 78-79 0.0 0.0 0.0 2.936419924221421 0.020462856614783424 80-81 0.0 0.0 0.0 3.2176191796374773 0.021452994838079394 82-83 0.0 0.0 0.0 3.5608670970467475 0.02310322521023935 84-85 0.0 0.0 0.0 4.094551599403276 0.02310322521023935 86-87 0.0 0.0 0.0 4.686654256934268 0.02310322521023935 88-89 0.0 0.0 0.0 5.377110644645993 0.02310322521023935 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGCTAG 50 0.0016080858 38.015858 1 TGCTAGC 50 0.0016080858 38.015858 2 CTAGCCA 55 0.0025673262 34.559868 4 GCTAGCC 65 0.0058163917 29.242966 3 TACAGAG 65 0.0058163917 29.242966 2 GCGCTTG 65 0.0058352067 29.223658 9 CATGGGC 100 4.9111404E-5 28.511894 4 GTCCAAG 155 4.514004E-8 27.592154 1 GAGTACT 1160 0.0 24.56299 12-13 TGCGACT 60 1.7095849E-4 23.744223 52-53 GTACTTT 1220 0.0 23.160349 14-15 CGACTGG 65 2.939422E-4 21.917744 54-55 GATCCAT 55 0.0028628332 21.603487 42-43 CAGGAGC 110 0.0028590325 21.599918 4 GACTGGT 110 0.00287002 21.585657 6 ACTTTTT 1310 0.0 21.569178 16-17 TGCAGAC 155 3.86521E-5 21.460564 2 AGTACTT 1135 0.0 21.33842 12-13 GAAGCGA 70 4.8375584E-4 20.358912 82-83 CATGGGG 430 0.0 19.89202 4 >>END_MODULE