FastQCFastQC Report
Thu 26 May 2016
SRR937810_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937810_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151005
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15881.0516208072580377No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13750.9105658753021424No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13640.9032813482997252No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12140.8039468891758552No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11040.7311016191516837No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8980.5946822952882355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7670.5079302009867224No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5760.38144432303566106No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC5210.3450216880235754No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA4910.32515479619880133No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT4810.3185324989238767No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC4770.3158835800139068No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4590.3039634449190424No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT4470.2960166881891328No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA4390.2907188503691931No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG4390.2907188503691931No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4190.27747425581934376No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC3580.23707824244230324No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT3520.23310486407734843No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG3210.21257574252508196No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3120.20661567497764974No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG2970.19668222906526275No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA2890.191384391245323No Hit
GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG2860.1893977020628456No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.1854243236978908No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG2750.18211317506042846No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC2680.1774775669679812No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC2600.17217972914804147No Hit
GTTCCAGTAGTGACTGATTCACACTCTTTTCCAAGTGCAGTGCACACTCC2400.1589351345981921No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.15429952650574483No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC2040.1350948644084633No Hit
GATAATACGTCTCAATGAAGTCACATAAGTGGGGATCATTCTTGTCAGTA2010.1331081752259859No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1940.1284725671335386No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC1890.1251614184960763No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1780.11787689149365915No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG1740.11522797258368928No Hit
CCCTGGGACACGGTGATGAGAGCTAAGCTGACTTCCCCAAAGCCACGTGA1730.1145657428561968No Hit
CTCCATTGCATTCAGCCCGCTCTCCCAGTCATCACGGTCTGGTTTCTTTA1670.110592364491242No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG1580.10463229694380981No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG1570.10397006721631734No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACACC352.7790485E-454.2900736
GTATGGA502.8083563E-547.5195961
TATGTCT1650.043.199634
TCTATGT1650.043.199632
GTCTATG1850.041.098031
ATGTCTT1850.041.084385
GTCTTGT1800.039.5865147
GTAAGCG308.272259E-539.58651494-95
CTATGTC1900.037.515473
CTTGTTA2100.036.1933869
AGACCAG650.00582588229.233125
CACCTAC856.3781615E-427.943427
GGTTTAG700.00835201327.1540531
TGTCTTG2800.025.4484736
TACTGGA1701.1049269E-725.1574332
CCTACCT950.001221038325.002019
CTACTGG1551.3920762E-624.5262431
AGTACTT9400.024.50994912-13
GAACTGC1652.4049823E-623.0321547
TCTTGTT3350.022.688398
Produced by FastQC (version 0.11.5)