##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937810_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 151005 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.65397834508791 33.0 31.0 34.0 30.0 34.0 2 31.74372371775769 33.0 31.0 34.0 30.0 34.0 3 31.77059037780206 33.0 31.0 34.0 30.0 34.0 4 35.3648223568756 37.0 35.0 37.0 33.0 37.0 5 35.3294857786166 37.0 35.0 37.0 33.0 37.0 6 35.4461640343035 37.0 35.0 37.0 33.0 37.0 7 35.38557663653521 37.0 35.0 37.0 33.0 37.0 8 35.38282176086884 37.0 35.0 37.0 33.0 37.0 9 36.97050428793749 39.0 37.0 39.0 33.0 39.0 10-11 36.86314360451641 39.0 37.0 39.0 33.0 39.0 12-13 36.74545544849508 39.0 37.0 39.0 33.0 39.0 14-15 37.82288997053078 40.0 38.0 41.0 33.0 41.0 16-17 37.757170292374425 40.0 38.0 41.0 33.0 41.0 18-19 37.71311214860435 40.0 37.5 41.0 33.0 41.0 20-21 37.52930697659018 40.0 37.0 41.0 32.0 41.0 22-23 37.40225158107347 40.0 37.0 41.0 32.0 41.0 24-25 37.21414853812787 39.5 36.5 41.0 32.0 41.0 26-27 37.22533028707659 39.0 36.5 41.0 32.0 41.0 28-29 37.14978643091288 39.0 36.0 41.0 32.0 41.0 30-31 36.89314923346909 39.0 36.0 41.0 31.0 41.0 32-33 36.57466971292341 39.0 35.0 40.5 31.0 41.0 34-35 36.229293731995625 38.5 35.0 40.0 30.0 41.0 36-37 36.17184861428429 39.0 35.0 40.0 30.0 41.0 38-39 36.09783119764246 39.0 35.0 40.0 30.0 41.0 40-41 35.85719678156352 38.5 35.0 40.5 29.5 41.0 42-43 35.49940730439389 38.0 35.0 40.0 28.5 41.0 44-45 35.133757160358925 38.0 35.0 40.0 26.5 41.0 46-47 34.61021158239794 38.0 34.5 40.0 24.0 41.0 48-49 34.097778219264264 38.0 34.0 40.0 22.5 41.0 50-51 33.07345452137346 37.0 32.0 39.5 20.0 40.5 52-53 33.113479686103105 37.0 33.0 39.5 18.5 40.5 54-55 33.31253931989007 37.0 33.0 40.0 16.5 41.0 56-57 32.92139333134665 37.0 33.0 40.0 11.0 41.0 58-59 32.64031323466111 36.0 33.0 40.0 8.5 41.0 60-61 32.2307142147611 36.0 32.0 39.0 6.5 41.0 62-63 31.839137776894805 35.0 31.5 39.0 2.0 41.0 64-65 31.43327042150922 35.0 31.0 39.0 2.0 40.0 66-67 30.9351511539353 35.0 31.0 38.0 2.0 40.0 68-69 30.453253203536306 35.0 30.0 37.0 2.0 40.0 70-71 29.91078109996358 35.0 29.5 37.0 2.0 39.0 72-73 29.44017085526969 34.0 29.0 36.0 2.0 39.0 74-75 28.974484288599715 34.0 29.0 36.0 2.0 38.5 76-77 28.54639912585676 34.0 28.5 35.0 2.0 37.0 78-79 28.021482732359857 34.0 27.0 35.0 2.0 37.0 80-81 27.497582861494653 33.5 26.0 35.0 2.0 36.0 82-83 27.007936823284 33.0 25.5 35.0 2.0 36.0 84-85 26.5756763021092 33.0 24.5 35.0 2.0 35.0 86-87 26.153918744412437 33.0 23.5 35.0 2.0 35.0 88-89 25.6341876096818 32.0 21.5 35.0 2.0 35.0 90-91 25.178030528790437 32.0 19.5 35.0 2.0 35.0 92-93 24.656839177510676 32.0 17.0 34.0 2.0 35.0 94-95 24.004016423297244 31.0 4.5 34.0 2.0 35.0 96-97 23.27160358928512 31.0 2.0 34.0 2.0 35.0 98-99 22.37895102811165 31.0 2.0 34.0 2.0 35.0 100-101 20.00768517598755 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.48178879909013617 1101 2 0.7515064026800964 1101 3 0.6243987685182262 1101 4 0.7017618806671067 1101 5 0.698177124021008 1101 6 0.6751052374011621 1101 7 0.6608047471037821 1101 8 0.5685056211842152 1101 9 0.6447437771959628 1101 10-11 0.43256258519323154 1101 12-13 0.5397531624091414 1101 14-15 0.7930739085287755 1101 16-17 0.9238288048638523 1101 18-19 0.8922745424640084 1101 20-21 1.0071336303537564 1101 22-23 1.2302041853713206 1101 24-25 1.2468659596990221 1101 26-27 1.260152254893093 1101 28-29 1.1168189431706566 1101 30-31 1.1164817093485269 1101 32-33 1.598660582629364 1101 34-35 1.389984648733801 1101 36-37 1.584661092723735 1101 38-39 1.3473181684787718 1101 40-41 2.074351587157956 1101 42-43 2.3087450388591577 1101 44-45 2.387364554978653 1101 46-47 2.414003507599382 1101 48-49 2.680155116757362 1101 50-51 2.956299180950367 1101 52-53 2.73372853432587 1101 54-55 2.7960662795934113 1101 56-57 2.8425035779288166 1101 58-59 2.916035935722377 1101 60-61 2.7550440240349943 1101 62-63 2.755628702871352 1101 64-65 2.4577806002479328 1101 66-67 2.469661254113479 1101 68-69 2.4615826310892857 1101 70-71 2.669772451668738 1101 72-73 2.539965776803836 1101 74-75 2.2472730452619025 1101 76-77 1.6934903060974875 1101 78-79 1.7970069625682292 1101 80-81 2.072360370081416 1101 82-83 2.2003491874965846 1101 84-85 2.3341455385111978 1101 86-87 2.694579051706839 1101 88-89 2.8116284505994997 1101 90-91 2.6360554975361943 1101 92-93 3.213027136091071 1101 94-95 3.0820415457004167 1101 96-97 2.913652217961019 1101 98-99 3.1963240336586765 1101 100-101 4.066223677036984 1102 1 0.10292477155140745 1102 2 0.06926888116546337 1102 3 -0.32331207485527 1102 4 -0.6162415616736823 1102 5 -0.4293960257208198 1102 6 -0.39425792955236716 1102 7 -0.2397460274228962 1102 8 0.03415106008267088 1102 9 -0.3820720575716834 1102 10-11 -0.08910695009075909 1102 12-13 0.07352252557609518 1102 14-15 0.4778415505253335 1102 16-17 0.43074790985524203 1102 18-19 0.2773519951318235 1102 20-21 0.2748444737272493 1102 22-23 0.08972225766047615 1102 24-25 0.5619262006628745 1102 26-27 0.6485687781288902 1102 28-29 0.10465026847186465 1102 30-31 -0.47379367791481286 1102 32-33 -0.5849194517940361 1102 34-35 -0.08956784696327702 1102 36-37 -0.18348004324872136 1102 38-39 -0.1920449984747563 1102 40-41 -0.044701769124316115 1102 42-43 0.0301736102877328 1102 44-45 -0.4080743434722933 1102 46-47 -0.6999723960150774 1102 48-49 -0.49841631181406854 1102 50-51 -0.144251593576314 1102 52-53 -0.16461914209408945 1102 54-55 -0.6913519648127888 1102 56-57 -0.8762916027940797 1102 58-59 -0.95909315205731 1102 60-61 -0.9293449604744772 1102 62-63 -0.8086914348222827 1102 64-65 -1.0478132035386416 1102 66-67 -1.1550203293632855 1102 68-69 -0.9027891417162195 1102 70-71 -0.8195562918768715 1102 72-73 -1.3392424848140614 1102 74-75 -1.5483379702286335 1102 76-77 -0.8914666646426141 1102 78-79 -0.6187761699618903 1102 80-81 -0.08323343370515701 1102 82-83 0.052776037754757255 1102 84-85 -0.3268183169104937 1102 86-87 -0.537331447432571 1102 88-89 0.09806562788004669 1102 90-91 0.5554014527857625 1102 92-93 0.5444901309275636 1102 94-95 -0.03711508080560577 1102 96-97 -0.10868857377736418 1102 98-99 -0.064364434499673 1102 100-101 -0.17415498045010835 1103 1 -0.4305314496467503 1103 2 -0.129993791645596 1103 3 -0.6033120748552712 1103 4 -0.2350434049916572 1103 5 -0.4411932607438587 1103 6 -0.0643040125016725 1103 7 -0.14739579700815142 1103 8 -0.3256646081200927 1103 9 -0.4393992465117762 1103 10-11 -0.2099364431783144 1103 12-13 0.10928289423969062 1103 14-15 0.2893622878525193 1103 16-17 -0.3072244403751725 1103 18-19 -0.09573556247185877 1103 20-21 0.11226382856595762 1103 22-23 -0.21931000040403603 1103 24-25 -0.5219447670790558 1103 26-27 -0.2944726965254816 1103 28-29 -0.1398658605603984 1103 30-31 -0.013425014320340267 1103 32-33 0.23729252977278037 1103 34-35 0.13273630050215957 1103 36-37 -0.16467819993074784 1103 38-39 0.6708121443823885 1103 40-41 0.4845148207374379 1103 42-43 0.4942289098269015 1103 44-45 0.21404547219591308 1103 46-47 0.48454373301718334 1103 48-49 0.24628414901542328 1103 50-51 0.13519541103197952 1103 52-53 0.42929790859715666 1103 54-55 -0.024163024720621706 1103 56-57 0.20177291333495617 1103 58-59 0.1648699815832373 1103 60-61 0.368627389755936 1103 62-63 -0.31403705694209805 1103 64-65 0.3508043079820986 1103 66-67 0.05963404851689802 1103 68-69 -0.3746785426378807 1103 70-71 0.13067412286967084 1103 72-73 0.5051814783195745 1103 74-75 0.13691548599256365 1103 76-77 -0.19966942961957557 1103 78-79 0.0367077010058523 1103 80-81 -0.36848688992635914 1103 82-83 -0.5970857133973162 1103 84-85 -0.7681086394911354 1103 86-87 -0.5065480372943263 1103 88-89 -1.0302293029955294 1103 90-91 -0.8827552292418837 1103 92-93 -0.8382748460309628 1103 94-95 -0.25066346790238114 1103 96-97 0.32799345387240564 1103 98-99 0.40623464384134067 1103 100-101 1.6531261255406768 1104 1 0.16271384154181945 1104 2 0.001809629008224789 1104 3 0.16984228756083652 1104 4 0.16638547571845663 1104 5 0.2794822101372816 1104 6 0.07880055558665333 1104 7 0.2455463982722108 1104 8 0.24688356247960286 1104 9 0.23369016678114463 1104 10-11 0.21628135288143113 1104 12-13 0.3138293328627668 1104 14-15 0.3850323463067298 1104 16-17 0.3189933556845759 1104 18-19 0.36609600280200283 1104 20-21 0.7771263529218757 1104 22-23 0.4520041368551091 1104 24-25 0.7854161335487859 1104 26-27 0.3611670523379047 1104 28-29 0.3112274496799188 1104 30-31 0.13704045439583012 1104 32-33 -0.0015253961852224052 1104 34-35 -0.3533645871358573 1104 36-37 0.1459734562718893 1104 38-39 0.5412819430401044 1104 40-41 0.24078241112496812 1104 42-43 0.2902886630202346 1104 44-45 0.6084165481863835 1104 46-47 0.11130277177016268 1104 48-49 0.493913506019112 1104 50-51 0.27594016097785357 1104 52-53 0.13051029222997812 1104 54-55 -0.02901255925190327 1104 56-57 -0.02743254239139503 1104 58-59 -0.22601549146287425 1104 60-61 -0.4857783257669013 1104 62-63 -0.3481353466151873 1104 64-65 -0.3728371728579134 1104 66-67 -0.4039944424984725 1104 68-69 -0.20470668725792507 1104 70-71 0.03797007437432498 1104 72-73 -0.3335281990997778 1104 74-75 -0.5051931955402331 1104 76-77 0.06354771694894623 1104 78-79 -0.16723254580081814 1104 80-81 0.39778286543194596 1104 82-83 0.4158824615323198 1104 84-85 0.09318168308950803 1104 86-87 0.2821508152711658 1104 88-89 0.4319486576020033 1104 90-91 0.41789426198998214 1104 92-93 1.1171267560474085 1104 94-95 1.408656731274931 1104 96-97 1.2536700072389344 1104 98-99 2.0841302922500873 1104 100-101 1.8325372534904467 1105 1 -0.17667840305176696 1105 2 0.034387928784511246 1105 3 -0.011645408188602602 1105 4 -0.03024949818161815 1105 5 0.23147699015219558 1105 6 -0.0347341200285598 1105 7 -0.2874047575816263 1105 8 -0.22449973356812336 1105 9 0.1024914344918102 1105 10-11 0.1987105102266966 1105 12-13 0.3561018906554594 1105 14-15 0.09758361401739535 1105 16-17 -0.03885526474793011 1105 18-19 -0.4846916556618339 1105 20-21 0.042483362616131615 1105 22-23 0.026388924327150676 1105 24-25 0.11956508955176304 1105 26-27 -0.051887571077458006 1105 28-29 -0.3940997315281365 1105 30-31 0.21529362367248694 1105 32-33 -0.07511786449245506 1105 34-35 -0.1499249898204198 1105 36-37 -0.06669432896300975 1105 38-39 -0.1958545222842858 1105 40-41 -0.47668589610844236 1105 42-43 -0.9030605166963994 1105 44-45 -0.836447359345307 1105 46-47 -0.7015001737928515 1105 48-49 -1.0490115499093093 1105 50-51 -0.4016325459572698 1105 52-53 -0.7931707293956762 1105 54-55 -0.9558162505270715 1105 56-57 -1.0680971583496373 1105 58-59 -0.44064077110493116 1105 60-61 -0.8997417858713064 1105 62-63 -1.0332350856159316 1105 64-65 -0.8107100289354712 1105 66-67 -0.42692509126804623 1105 68-69 -1.0713407290178054 1105 70-71 -0.5463420061625861 1105 72-73 -0.8647781990997778 1105 74-75 -0.4729411448318075 1105 76-77 -0.003589680515631244 1105 78-79 0.0352516078158871 1105 80-81 -0.626685814657538 1105 82-83 0.03226016473888649 1105 84-85 0.5654039053117295 1105 86-87 0.5324304573293333 1105 88-89 -0.07709310227868116 1105 90-91 -0.022495372611064823 1105 92-93 0.9741131468005761 1105 94-95 0.15399603030550324 1105 96-97 0.14938682304803308 1105 98-99 0.36002048613524806 1105 100-101 0.12830533701021096 1106 1 0.1053264278661068 1106 2 0.19109082733523763 1106 3 0.14596328746356946 1106 4 -0.037193942626061016 1106 5 -0.13506890356760692 1106 6 0.26599051765260384 1106 7 0.4496121506309336 1106 8 0.12996886546569186 1106 9 0.1209093088879456 1106 10-11 -0.004489972865087566 1106 12-13 0.0731912626361364 1106 14-15 0.5258746768193276 1106 16-17 0.496772754575737 1106 18-19 0.7832733201835822 1106 20-21 0.45658360416202726 1106 22-23 0.29812805476193205 1106 24-25 -0.12937814716320872 1106 26-27 0.32960397481625847 1106 28-29 0.8645053409356294 1106 30-31 0.36595787488021614 1106 32-33 -0.11104781618327308 1106 34-35 -0.33923658402331824 1106 36-37 -0.11107838693402527 1106 38-39 -2.023483712392249E-4 1106 40-41 -0.3323622246108542 1106 42-43 -0.3470520625901159 1106 44-45 -0.8548652337414424 1106 46-47 -0.26475500471072877 1106 48-49 -0.283612999184669 1106 50-51 -0.5121397923340822 1106 52-53 -0.7812444008932644 1106 54-55 -0.2660517577734467 1106 56-57 -0.1244075448230646 1106 58-59 -0.19698135081507218 1106 60-61 -0.5839205298326533 1106 62-63 -0.007117935857475999 1106 64-65 -0.12079456999827087 1106 66-67 0.13466911163050455 1106 68-69 0.2630493676005514 1106 70-71 0.33499857354755846 1106 72-73 0.2969065835089175 1106 74-75 0.7202955701440388 1106 76-77 0.9449308508853349 1106 78-79 0.836731076414921 1106 80-81 1.2908866491105755 1106 82-83 0.31637852222680607 1106 84-85 0.6402831323648677 1106 86-87 0.5451116167496224 1106 88-89 0.9235107624556171 1106 90-91 1.1148838544420805 1106 92-93 0.6098317458343949 1106 94-95 0.11172549890453709 1106 96-97 0.949054697444172 1106 98-99 1.325449230096602 1106 100-101 1.067314998845962 1107 1 0.14823410788436675 1107 2 0.1454443592307051 1107 3 -0.008036484304088987 1107 4 -0.11068475627435959 1107 5 0.1974656165738935 1107 6 0.24938661487957603 1107 7 0.05910661599667577 1107 8 -0.2591672751254279 1107 9 -0.06652650076628674 1107 10-11 0.12232162133781088 1107 12-13 -0.16188804810219892 1107 14-15 -0.27345203777613136 1107 16-17 -0.13323409239448125 1107 18-19 0.08767820435566875 1107 20-21 0.35216730837291976 1107 22-23 0.5742891867943385 1107 24-25 -0.15933364185681143 1107 26-27 -0.02238735235479794 1107 28-29 -0.08479855042577356 1107 30-31 -0.19224137195305957 1107 32-33 -0.14111742704713492 1107 34-35 0.614346663722884 1107 36-37 0.6345851985960422 1107 38-39 0.8251428530438076 1107 40-41 0.7944208405494777 1107 42-43 0.7437844089378984 1107 44-45 0.7275836992723654 1107 46-47 1.1444370528038306 1107 48-49 1.3592889750250805 1107 50-51 1.4294041994495572 1107 52-53 1.7829174158361738 1107 54-55 1.7865557967170247 1107 56-57 1.938983987757105 1107 58-59 1.911885475614227 1107 60-61 2.1788890102791783 1107 62-63 1.9165954043228233 1107 64-65 2.0528617120969024 1107 66-67 2.5816050924589895 1107 68-69 2.4456922981038076 1107 70-71 2.203165867853162 1107 72-73 2.548361564679748 1107 74-75 2.547837507836608 1107 76-77 2.9908055513303893 1107 78-79 3.2468931213766936 1107 80-81 3.5001186472847188 1107 82-83 3.9201101209943516 1107 84-85 3.801843100412345 1107 86-87 3.6292152342322197 1107 88-89 3.2049497673626135 1107 90-91 3.184580305429183 1107 92-93 3.4831627968443257 1107 94-95 4.385404604233763 1107 96-97 4.579860357606215 1107 98-99 4.7975478264677065 1107 100-101 5.224652668593762 1108 1 0.03186697438674102 1108 2 0.12615001529850645 1108 3 0.18905433735847055 1108 4 -0.02701582557772042 1108 5 -0.14712775963575808 1108 6 -0.4819351378402601 1108 7 -0.08484962611428415 1108 8 -0.10246835104055663 1108 9 0.06564877121785173 1108 10-11 0.12232162133781088 1108 12-13 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-1.9775934419061052 2316 64-65 -2.030693959146671 2316 66-67 -2.1535091133066153 2316 68-69 -2.366307441598156 2316 70-71 -2.531362667319609 2316 72-73 -2.7012967941411006 2316 74-75 -2.3299200245379588 2316 76-77 -2.580581186484409 2316 78-79 -2.221090219548671 2316 80-81 -2.0421472471644257 2316 82-83 -2.6126548949489035 2316 84-85 -2.4688017879848694 2316 86-87 -2.628382214846976 2316 88-89 -2.644012294197875 2316 90-91 -2.7145867408387936 2316 92-93 -2.721106091032297 2316 94-95 -2.3496770642766833 2316 96-97 -2.028098255005226 2316 98-99 -2.749110597427652 2316 100-101 -2.1707075188208265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 939.0 3 190.0 4 148.0 5 148.0 6 180.0 7 206.0 8 259.0 9 325.0 10 393.0 11 497.0 12 631.0 13 893.0 14 1137.0 15 1352.0 16 1778.0 17 2286.0 18 2028.0 19 1433.0 20 1483.0 21 1563.0 22 1613.0 23 1661.0 24 1833.0 25 2076.0 26 2279.0 27 2639.0 28 3076.0 29 3640.0 30 4409.0 31 5363.0 32 6681.0 33 8839.0 34 11976.0 35 16976.0 36 21840.0 37 23993.0 38 13345.0 39 897.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.54566589173032 17.88442377947526 15.201594154765857 21.36831617402856 2 16.89494685265471 21.606171864107452 36.07247425623593 25.426407027001908 3 20.711563740655663 23.386400754185864 30.70651813099996 25.19551737415851 4 13.877952102408159 17.2853604934501 35.10852316865078 33.72816423549096 5 14.351633613735501 36.25951917778206 32.4306021232663 16.958245085216145 6 31.245437774563023 33.90911383333112 16.570002787104997 18.27544560500086 7 26.97873469793982 33.6376604850214 20.432604584812395 18.951000232226388 8 26.070859872611464 29.998009554140125 20.267383227176218 23.663747346072185 9 25.264764432647645 17.857332448573324 21.65892501658925 35.21897810218978 10-11 26.228812012912687 24.852940688566036 25.246757727871433 23.671489570649847 12-13 27.357598558816797 22.95210321844343 26.89810529528663 22.792192927453147 14-15 24.14676305775171 25.227679149068877 27.048780568725096 23.576777224454318 16-17 21.716990771149725 28.160599244972566 27.26956071734241 22.852849266535298 18-19 21.05593152866242 26.646098726114648 30.05175159235669 22.24621815286624 20-21 23.161382988957186 24.754945051299828 29.12231351774513 22.961358441997856 22-23 22.116319265896777 24.582903436630186 30.425406978094344 22.875370319378693 24-25 23.415195676765936 25.26796288494266 29.322155911106982 21.994685527184423 26-27 22.220968999319943 24.779312975833072 31.426628406508648 21.573089618338336 28-29 20.97796298631064 26.949058068625952 30.315326374742043 21.757652570321365 30-31 21.637609276247037 25.406167576265283 30.040973408755374 22.915249738732314 32-33 22.166114808597044 25.55091803082885 30.1271374823328 22.15582967824131 34-35 23.215523905261445 25.664606411672786 28.643516168522886 22.47635351454288 36-37 22.763823096214324 25.07861948170853 30.1523957379616 22.00516168411555 38-39 22.067730272977546 25.78858336926598 29.61789777438721 22.525788583369266 40-41 21.81400269984312 26.01268992600307 29.736417036209073 22.43689033794474 42-43 22.41090473615594 26.00390781795805 27.98667759174913 23.59850985413688 44-45 22.037046865877606 25.492940926702406 28.355713603311926 24.11429860410806 46-47 23.141403285301067 26.396518323912638 28.277008199984078 22.185070190802218 48-49 23.16834442623277 25.354187541888688 28.55763552028242 22.919832511596116 50-51 21.489212762216304 26.484883269579758 28.827122197856152 23.198781770347786 52-53 22.630227171669574 27.029313972086356 26.701132871866683 23.639325984377386 54-55 21.93815267438929 25.800790839005877 27.52842906996802 24.73262741663681 56-57 23.010742702491154 25.763580945052684 27.274325305880126 23.951351046576033 58-59 23.921089987662018 25.015256643273148 27.77007575255051 23.293577616514323 60-61 23.105443812492744 25.608635813421326 29.208549587941697 22.07737078614423 62-63 21.670707399017623 25.951119852212013 28.82827606106536 23.549896687705008 64-65 21.706714425484634 26.64112896539144 28.120325688605874 23.53183092051805 66-67 22.38208000849037 25.789505104172882 27.18975318223124 24.6386617051055 68-69 21.507908343476487 25.683792290318625 28.319796230394967 24.48850313580992 70-71 23.15996497698298 25.851696095729583 27.327571339497737 23.660767587789703 72-73 22.632704840555196 25.064092997031658 28.82410493673615 23.479097225677 74-75 21.77765246380177 27.144112915263797 27.18026968304778 23.897964937886655 76-77 21.923769657125987 26.028174246469927 27.092254569939733 24.955801526464356 78-79 22.588499097760323 25.5930899055302 27.72463114319074 24.093779853518736 80-81 23.090241499746263 26.111890570531127 27.0535438868822 23.744324042840418 82-83 22.562599275105203 25.20352432841117 28.223808781639537 24.010067614844093 84-85 22.415954264794635 25.39577267692879 27.441786431977505 24.74648662629907 86-87 22.61343361787081 25.433604605660236 27.34809744579528 24.60486433067367 88-89 22.357463377100615 25.31216938236065 26.846732710044808 25.48363453049393 90-91 22.647523149990715 24.508477274536336 27.43539228952745 25.4086072859455 92-93 23.19016980631467 24.506832050941895 27.99217299018307 24.310825152560362 94-95 22.621760111437244 24.466112797041607 27.805581811850153 25.106545279670993 96-97 21.66806523404721 24.932421003054735 28.467424435901943 24.93208932699611 98-99 22.42142203234666 25.319751628610476 26.881028512292527 25.377797826750342 100-101 22.580784230493727 23.6399994693975 27.98165441859231 25.79756188151646 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 615.0 1 407.0 2 229.5 3 253.5 4 209.5 5 200.0 6 284.5 7 480.5 8 662.0 9 708.5 10 601.5 11 452.5 12 394.0 13 389.5 14 426.0 15 433.0 16 435.0 17 444.0 18 444.5 19 458.0 20 496.5 21 517.0 22 571.5 23 678.5 24 698.0 25 679.0 26 697.0 27 806.5 28 912.0 29 972.5 30 1085.5 31 1227.0 32 1357.0 33 1422.0 34 1563.5 35 1617.0 36 1550.0 37 1643.0 38 1915.0 39 2071.5 40 2229.0 41 2851.0 42 3469.0 43 3866.5 44 4072.0 45 4282.0 46 4668.5 47 6191.5 48 9513.5 49 9654.5 50 6824.0 51 5517.0 52 5304.0 53 5446.0 54 5564.0 55 5242.5 56 4602.0 57 4138.0 58 3851.0 59 3603.5 60 3334.5 61 3072.0 62 2706.5 63 2230.5 64 1751.5 65 1355.0 66 1046.0 67 934.0 68 731.5 69 477.5 70 399.0 71 334.0 72 293.0 73 232.0 74 171.0 75 135.5 76 86.5 77 54.5 78 30.0 79 13.0 80 8.5 81 6.5 82 3.5 83 1.5 84 2.5 85 3.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30131452600907255 2 0.2556206748120923 3 0.25098506671964504 4 0.2595940531770471 5 0.2569451342670772 6 0.20595344525015727 7 0.19204662097281547 8 0.18873547233535312 9 0.2019800668852025 10-11 0.1990000331114864 12-13 0.19568888447402405 14-15 0.19833780338399393 16-17 0.1867487831528757 18-19 0.18873547233535312 20-21 0.18111983046918975 22-23 0.1923777358365617 24-25 0.1884043574716069 26-27 0.18707989801662195 28-29 0.20131783715771004 30-31 0.197013343929009 32-33 0.1999933777027251 34-35 0.19568888447402405 36-37 0.18476209397039833 38-39 0.17217972914804147 40-41 0.16919969537432536 42-43 0.18641766828912948 44-45 0.1834376345154134 46-47 0.18078871560544352 48-49 0.20396675606767986 50-51 0.19502665474653158 52-53 0.21588689116254425 54-55 0.18476209397039833 56-57 0.16655077646435548 58-59 0.16621966160060925 60-61 0.15727956027946094 62-63 0.16456408728187807 64-65 0.1639018575543856 66-67 0.1629085129631469 68-69 0.16323962782689314 70-71 0.16357074269063937 72-73 0.1639018575543856 74-75 0.18045760074169728 76-77 0.17515976292175756 78-79 0.17813979669547367 80-81 0.17184861428429524 82-83 0.14867057382205887 84-85 0.14833945895831263 86-87 0.1549617562332373 88-89 0.16257739809940067 90-91 0.1648952021456243 92-93 0.16224628323565443 94-95 0.1639018575543856 96-97 0.16919969537432536 98-99 0.17416641833051885 100-101 0.15429952650574483 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 151005.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.93695572994272 #Duplication Level Percentage of deduplicated Percentage of total 1 73.49390326616124 32.29098374226019 2 13.091775061419506 11.50425482599914 3 5.091413326902497 6.711036058408662 4 2.5833873423063594 4.540247011688354 5 1.4228224335689632 3.125724313764445 6 0.8350038434292433 2.2012516141849607 7 0.5938474987565376 1.8264295884242245 8 0.42503805748564366 1.4939902652230057 9 0.30747433945769964 1.2158537796761697 >10 1.8945845328349438 16.060395351147314 >50 0.17182389557930275 5.158107347438826 >100 0.07536135771022051 7.6421310552630715 >500 0.006028908616817641 1.8290785073341944 >1k 0.00753613577102205 4.400516539187445 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1588 1.0516208072580377 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1375 0.9105658753021424 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1364 0.9032813482997252 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1214 0.8039468891758552 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1104 0.7311016191516837 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 898 0.5946822952882355 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 767 0.5079302009867224 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 576 0.38144432303566106 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 521 0.3450216880235754 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 491 0.32515479619880133 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 481 0.3185324989238767 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 477 0.3158835800139068 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 459 0.3039634449190424 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 447 0.2960166881891328 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 439 0.2907188503691931 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 439 0.2907188503691931 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 419 0.27747425581934376 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 358 0.23707824244230324 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 352 0.23310486407734843 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 321 0.21257574252508196 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 312 0.20661567497764974 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 297 0.19668222906526275 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 289 0.191384391245323 No Hit GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG 286 0.1893977020628456 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 280 0.1854243236978908 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 275 0.18211317506042846 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 268 0.1774775669679812 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 260 0.17217972914804147 No Hit GTTCCAGTAGTGACTGATTCACACTCTTTTCCAAGTGCAGTGCACACTCC 240 0.1589351345981921 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 233 0.15429952650574483 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 204 0.1350948644084633 No Hit GATAATACGTCTCAATGAAGTCACATAAGTGGGGATCATTCTTGTCAGTA 201 0.1331081752259859 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 194 0.1284725671335386 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 189 0.1251614184960763 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 178 0.11787689149365915 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 174 0.11522797258368928 No Hit CCCTGGGACACGGTGATGAGAGCTAAGCTGACTTCCCCAAAGCCACGTGA 173 0.1145657428561968 No Hit CTCCATTGCATTCAGCCCGCTCTCCCAGTCATCACGGTCTGGTTTCTTTA 167 0.110592364491242 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 158 0.10463229694380981 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 157 0.10397006721631734 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.622297274924672E-4 2 0.0 0.0 0.0 0.0 6.622297274924672E-4 3 0.0 0.0 0.0 0.0 6.622297274924672E-4 4 0.0 0.0 0.0 0.0 6.622297274924672E-4 5 0.0 0.0 0.0 0.0 6.622297274924672E-4 6 0.0 0.0 0.0 0.0 6.622297274924672E-4 7 0.0 0.0 0.0 0.0 0.0013244594549849343 8 0.0 0.0 0.0 0.0 0.0019866891824774015 9 0.0 0.0 0.0 0.0 0.0019866891824774015 10-11 0.0 0.0 0.0 0.0 0.0019866891824774015 12-13 0.0 0.0 0.0 0.0 0.0019866891824774015 14-15 3.311148637462336E-4 0.0 0.0 0.0 0.0026489189099698687 16-17 6.622297274924672E-4 0.0 0.0 0.0 0.0026489189099698687 18-19 6.622297274924672E-4 0.0 0.0 0.0 0.003642263501208569 20-21 6.622297274924672E-4 0.0 0.0 0.001655574318731168 0.003973378364954803 22-23 6.622297274924672E-4 0.0 0.0 0.0029800337737161025 0.00463560809244727 24-25 6.622297274924672E-4 0.0 0.0 0.003973378364954803 0.005297837819939737 26-27 6.622297274924672E-4 0.0 0.0 0.005960067547432204 0.005960067547432204 28-29 6.622297274924672E-4 0.0 0.0 0.015231283732326743 0.005960067547432204 30-31 6.622297274924672E-4 0.0 0.0 0.03708486473957816 0.006291182411178438 32-33 6.622297274924672E-4 0.0 0.0 0.06390516870302307 0.007284527002417138 34-35 6.622297274924672E-4 0.0 0.0 0.0927121618489454 0.007946756729909606 36-37 6.622297274924672E-4 0.0 0.0 0.13443263468097083 0.007946756729909606 38-39 6.622297274924672E-4 0.0 0.0 0.19866891824774013 0.007946756729909606 40-41 6.622297274924672E-4 0.0 0.0 0.25793847885831594 0.007946756729909606 42-43 6.622297274924672E-4 0.0 0.0 0.2970100327803715 0.007946756729909606 44-45 6.622297274924672E-4 0.0 0.0 0.33277043806496476 0.007946756729909606 46-47 6.622297274924672E-4 0.0 0.0 0.3718419919870203 0.008940101321148307 48-49 6.622297274924672E-4 0.0 0.0 0.40495347836164364 0.00927121618489454 50-51 6.622297274924672E-4 0.0 0.0 0.43111155259759615 0.009602331048640774 52-53 6.622297274924672E-4 0.0 0.0 0.47581205920333763 0.010926790503625708 54-55 6.622297274924672E-4 0.0 0.0 0.5625641535048509 0.012582364822356876 56-57 6.622297274924672E-4 0.0 0.0 0.6857388828184496 0.012582364822356876 58-59 6.622297274924672E-4 0.0 0.0 0.8138803350882421 0.012582364822356876 60-61 6.622297274924672E-4 0.0 0.0 0.9476507400417205 0.012582364822356876 62-63 6.622297274924672E-4 0.0 0.0 1.0393695572994273 0.01390682427734181 64-65 6.622297274924672E-4 0.0 0.0 1.1741333068441442 0.01390682427734181 66-67 6.622297274924672E-4 0.0 0.0 1.373795569683123 0.01390682427734181 68-69 6.622297274924672E-4 0.0 0.0 1.6410052647263336 0.01390682427734181 70-71 6.622297274924672E-4 0.0 0.0 1.916823946226946 0.014237939141088042 72-73 6.622297274924672E-4 0.0 0.0 2.195622661501275 0.015231283732326744 74-75 6.622297274924672E-4 0.0 0.0 2.4780636402768117 0.015231283732326744 76-77 6.622297274924672E-4 0.0 0.0 2.7667958014635277 0.015231283732326744 78-79 6.622297274924672E-4 0.0 0.0 3.0628124896526607 0.015231283732326744 80-81 6.622297274924672E-4 0.0 0.0 3.32571769146717 0.016224628323565447 82-83 6.622297274924672E-4 0.0 0.0 3.6813350551306248 0.018873547233535314 84-85 6.622297274924672E-4 0.0 0.0 4.185954107479885 0.019204662097281548 86-87 6.622297274924672E-4 0.0 0.0 4.739247044799841 0.019204662097281548 88-89 6.622297274924672E-4 0.0 0.0 5.4028012317472935 0.019535776961027783 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACACC 35 2.7790485E-4 54.290073 6 GTATGGA 50 2.8083563E-5 47.519596 1 TATGTCT 165 0.0 43.19963 4 TCTATGT 165 0.0 43.19963 2 GTCTATG 185 0.0 41.09803 1 ATGTCTT 185 0.0 41.08438 5 GTCTTGT 180 0.0 39.586514 7 GTAAGCG 30 8.272259E-5 39.586514 94-95 CTATGTC 190 0.0 37.51547 3 CTTGTTA 210 0.0 36.193386 9 AGACCAG 65 0.005825882 29.23312 5 CACCTAC 85 6.3781615E-4 27.94342 7 GGTTTAG 70 0.008352013 27.154053 1 TGTCTTG 280 0.0 25.448473 6 TACTGGA 170 1.1049269E-7 25.157433 2 CCTACCT 95 0.0012210383 25.00201 9 CTACTGG 155 1.3920762E-6 24.526243 1 AGTACTT 940 0.0 24.509949 12-13 GAACTGC 165 2.4049823E-6 23.032154 7 TCTTGTT 335 0.0 22.68839 8 >>END_MODULE