FastQCFastQC Report
Thu 26 May 2016
SRR937810_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937810_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151005
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21291.4098870898314626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14450.956921956226615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13930.9224860103970066No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13050.8642097943776695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9830.6509718221250952No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9720.643687295122678No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8460.5602463494586273No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC6800.45031621469487765No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG6090.40329790404291255No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA5840.3867421608556008No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC5190.3436972285685905No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT4880.3231681070163239No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4850.3211814178338465No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC4620.30595013410151983No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC4090.27085195854441907No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3960.26224297208701697No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT3890.2576073639945697No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.23906493162478065No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA3530.2337670938048409No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA3400.22515810734743882No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC3260.21588689116254425No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG3070.2033045263401874No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG2850.18873547233535312No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC2810.18608655342538327No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT2800.1854243236978908No Hit
GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG2660.17615310751299626No Hit
GATAATACGTCTCAATGAAGTCACATAAGTGGGGATCATTCTTGTCAGTA2630.17416641833051885No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG2580.17085526969305653No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC2460.1629085129631469No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG2150.14237939141088043No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC2000.13244594549849342No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG1970.13045925631601601No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.12383695904109135No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.12383695904109135No Hit
TCCTACGTCTATCTGTCTATGTCTTGTTATTTTGACCGAGATGATGTGGC1790.11853912122115162No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC1780.11787689149365915No Hit
AGATGATGTGGCTCTGAAGAACTTTGCCAAATACTTTCTCCACCAATCTC1770.11721466176616668No Hit
GGTGAAATCCATTAAAGAACTGGGTGACCACGTGACCAACTTACGCAAGA1740.11522797258368928No Hit
CCCTGGGACACGGTGATGAGAGCTAAGCTGACTTCCCCAAAGCCACGTGA1710.11324128340121188No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT1700.11257905367371941No Hit
GTTCCAGTAGTGACTGATTCACACTCTTTTCCAAGTGCAGTGCACACTCC1690.11191682394622694No Hit
ACCTGGAGTTGTATGCCTCCTACGTCTATCTGTCTATGTCTTGTTATTTT1590.10529452667130228No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC1570.10397006721631734No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGG250.00500925757.0103344
TGAGCGC459.6055784E-442.2158936
CTAGAGC600.003936686631.672413
TGTCCAC600.003936686631.672415
TTTGAGC953.46671E-530.0054384
CATGGGG4450.026.6902314
TAGAGCT950.001220333525.0045324
ATGGGGG2052.6255293E-823.1749325
GTACATG8000.022.5665911
AGTACTT12150.022.28061112-13
TACATGG8000.021.9727332
ACATGGG8100.021.7014663
ATGGGGA2205.547372E-821.5948245
TCTATGT1553.8721606E-521.4555022
GAGTACT12850.021.43647612-13
GTACTTT12900.021.16930814-15
CTGTACA1150.003704637820.6559184
ATGGGGC1150.003711758620.649087
TACTTTT13000.020.64113814-15
GTCTATG1707.884621E-519.562371
Produced by FastQC (version 0.11.5)