##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937810_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 151005 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06895135922652 31.0 31.0 34.0 30.0 34.0 2 32.25935565047515 34.0 31.0 34.0 30.0 34.0 3 32.318194761762854 34.0 31.0 34.0 30.0 34.0 4 35.99119896692162 37.0 35.0 37.0 35.0 37.0 5 35.88401046322969 37.0 35.0 37.0 35.0 37.0 6 35.9561074136618 37.0 35.0 37.0 35.0 37.0 7 35.90123505844177 37.0 35.0 37.0 35.0 37.0 8 35.89513592265157 37.0 35.0 37.0 35.0 37.0 9 37.50779113274395 39.0 37.0 39.0 35.0 39.0 10-11 37.45376312042647 39.0 37.0 39.0 34.5 39.0 12-13 37.35601470149995 39.0 37.0 39.0 34.0 39.0 14-15 38.45873315453131 40.0 38.0 41.0 34.0 41.0 16-17 38.42358200059601 40.0 38.0 41.0 33.5 41.0 18-19 38.433462468130195 40.0 38.0 41.0 34.0 41.0 20-21 38.32299592728718 40.0 38.0 41.0 34.0 41.0 22-23 38.16273302208536 40.0 37.5 41.0 33.5 41.0 24-25 38.153279692725405 40.0 37.0 41.0 33.5 41.0 26-27 37.93813118770902 40.0 37.0 41.0 33.0 41.0 28-29 37.771908214959765 40.0 37.0 41.0 33.0 41.0 30-31 37.755650475149835 40.0 37.0 41.0 33.0 41.0 32-33 37.56534551836032 40.0 36.0 41.0 33.0 41.0 34-35 37.3351246647462 39.0 35.0 41.0 33.0 41.0 36-37 37.08722227740803 39.0 35.0 41.0 32.5 41.0 38-39 36.86810039402668 39.0 35.0 41.0 32.0 41.0 40-41 36.64296215357108 39.0 35.0 41.0 31.0 41.0 42-43 36.2903215125327 39.0 35.0 41.0 30.5 41.0 44-45 35.827101751597624 39.0 35.0 40.0 29.5 41.0 46-47 35.25979934439257 38.0 35.0 40.0 27.5 41.0 48-49 34.717035859739745 38.0 34.5 40.0 24.5 41.0 50-51 34.27954041256912 38.0 34.0 40.0 23.5 41.0 52-53 33.9711863845568 38.0 34.0 40.0 22.5 41.0 54-55 33.65467037515314 37.0 33.0 40.0 21.5 41.0 56-57 33.23213800867521 37.0 33.0 40.0 19.5 41.0 58-59 32.90265223005861 36.0 33.0 40.0 17.0 41.0 60-61 32.4183139631138 35.5 32.0 39.0 12.0 41.0 62-63 32.01147644117744 35.0 32.0 39.0 7.5 40.0 64-65 31.613148571239364 35.0 31.0 38.5 4.0 40.0 66-67 31.083040296678917 35.0 31.0 38.0 2.0 40.0 68-69 30.609377172941294 35.0 30.5 37.0 2.0 39.5 70-71 30.120750968510976 34.5 30.0 36.5 2.0 39.0 72-73 29.615992847918942 34.0 29.0 36.0 2.0 38.5 74-75 29.133647892453894 34.0 29.0 35.0 2.0 37.5 76-77 27.96606072646601 32.5 27.0 34.5 2.0 36.0 78-79 28.55591205589219 34.0 29.0 35.0 2.0 36.0 80-81 28.355071024138276 34.0 29.0 35.0 2.0 36.0 82-83 27.9933710804278 34.0 28.0 35.0 2.0 36.0 84-85 27.644306479917883 33.5 27.0 35.0 2.0 35.0 86-87 27.275080295354456 33.0 26.5 35.0 2.0 35.0 88-89 26.935982252243303 33.0 25.5 35.0 2.0 35.0 90-91 26.437021952915465 33.0 25.0 35.0 2.0 35.0 92-93 25.99320221184729 33.0 24.0 35.0 2.0 35.0 94-95 25.51527763981325 32.0 21.5 34.5 2.0 35.0 96-97 24.939412602231712 32.0 18.5 34.0 2.0 35.0 98-99 24.12482699248369 32.0 3.5 34.0 2.0 35.0 100-101 21.754246548127547 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.45339419564206196 1101 2 0.32262863389310326 1101 3 0.4767119657195025 1101 4 0.19886498612678594 1101 5 0.30568939485176116 1101 6 -0.11306802490757661 1101 7 0.2193205029328169 1101 8 0.23289055978730033 1101 9 0.43063838239184093 1101 10-11 0.2742225292125724 1101 12-13 0.39519761622975125 1101 14-15 0.4762981988096797 1101 16-17 0.5841615055804752 1101 18-19 0.4382272020181617 1101 20-21 0.36589436038440937 1101 22-23 0.3410936379560283 1101 24-25 0.40013399906921876 1101 26-27 0.5066329851414295 1101 28-29 0.35498669022151574 1101 30-31 0.22149866812154784 1101 32-33 0.2612047937772317 1101 34-35 -0.23535236880298527 1101 36-37 -0.5231691008004375 1101 38-39 -0.8324302216294157 1101 40-41 -0.9776183009396107 1101 42-43 -0.6817961964679924 1101 44-45 -0.7789944042534884 1101 46-47 -0.6192516394971221 1101 48-49 -0.7321327132679727 1101 50-51 -0.6566436302133454 1101 52-53 -0.7049198556125731 1101 54-55 -0.48921399522073017 1101 56-57 -0.4369737127891469 1101 58-59 -0.5702104546853803 1101 60-61 -0.10605507332582675 1101 62-63 -0.26998345471651675 1101 64-65 -0.34623982803852016 1101 66-67 -0.2238964087149995 1101 68-69 -0.6741674211082582 1101 70-71 -0.672228319374991 1101 72-73 -0.5244365605236148 1101 74-75 -0.14453898152197908 1101 76-77 0.007797056260272939 1101 78-79 -0.01158832978456914 1101 80-81 0.1594663150044191 1101 82-83 0.44812305647581496 1101 84-85 0.3955102166667537 1101 86-87 0.08370087854656205 1101 88-89 0.7043367128050626 1101 90-91 0.619672362648398 1101 92-93 1.044866238400573 1101 94-95 1.3278741609688502 1101 96-97 0.0693812967376708 1101 98-99 0.27698442588434347 1101 100-101 1.114181985914211 1102 1 0.2020344710293216 1102 2 0.04624309172442764 1102 3 0.09265000358524844 1102 4 0.04578409111817905 1102 5 0.17422640001527157 1102 6 0.06548619195989147 1102 7 -0.05097209603448505 1102 8 0.11502481107817886 1102 9 0.10337504332127168 1102 10-11 -0.1571716188080785 1102 12-13 0.14032670400943914 1102 14-15 -0.00277236917656154 1102 16-17 -0.17738754777580112 1102 18-19 -2.925914414007025E-4 1102 20-21 0.024775599627098188 1102 22-23 -0.15157417615756685 1102 24-25 -0.40196582881374354 1102 26-27 -0.21861658456597155 1102 28-29 -0.2958566832724543 1102 30-31 -0.33147895666330385 1102 32-33 -0.1503614712829986 1102 34-35 0.0021519341229989664 1102 36-37 -0.5061983606971623 1102 38-39 -0.1715180013196047 1102 40-41 -0.24684377426147108 1102 42-43 -0.6517101379482071 1102 44-45 -0.5107499980572712 1102 46-47 -0.5757232401855887 1102 48-49 0.11125799241188616 1102 50-51 0.19027547477804063 1102 52-53 0.03841921495541101 1102 54-55 -0.00716580244964149 1102 56-57 -0.20668111382185117 1102 58-59 0.023249097810314368 1102 60-61 -0.6446437136012122 1102 62-63 -0.30678207778020194 1102 64-65 -0.3335375905170075 1102 66-67 -0.03189985105579041 1102 68-69 -0.37886621628898354 1102 70-71 -0.15524036756776383 1102 72-73 -0.07009921112602058 1102 74-75 0.18888614756580324 1102 76-77 0.047797056260272086 1102 78-79 0.14152698863195567 1102 80-81 -0.30826174007303564 1102 82-83 -0.5866273738167855 1102 84-85 -0.19338823427989027 1102 86-87 -0.08030944847581267 1102 88-89 0.14924204843329036 1102 90-91 -0.2680729041330103 1102 92-93 -0.29947111099701473 1102 94-95 -0.10393306794680868 1102 96-97 -0.7570386688389235 1102 98-99 -1.8005370887456067 1102 100-101 -1.4408610433456843 1103 1 -0.04920976860201165 1103 2 -0.13827303730783314 1103 3 -0.034170272912447786 1103 4 -0.03863987201545882 1103 5 0.10860428019960011 1103 6 -0.35515896933043223 1103 7 -0.04138684257826242 1103 8 -0.17363878339186556 1103 9 -0.2252885511487719 1103 10-11 -0.30288590452236264 1103 12-13 -0.1391203005988615 1103 14-15 -0.28304886687241293 1103 16-17 -0.06531381505691058 1103 18-19 -0.3225967389068316 1103 20-21 -0.27162993032681726 1103 22-23 -0.36042210242484884 1103 24-25 -0.19625154309945714 1103 26-27 -0.6101373218931556 1103 28-29 -0.8752115219821306 1103 30-31 -0.45055729767712904 1103 32-33 -0.4593937293475179 1103 34-35 -0.6078941488263112 1103 36-37 -0.26343338373863645 1103 38-39 -0.05161016721822165 1103 40-41 -0.086751608362853 1103 42-43 -0.27779308725695984 1103 44-45 -0.35775460635220213 1103 46-47 -1.5083499682961872 1103 48-49 -0.8515069845466385 1103 50-51 -0.4554388109362364 1103 52-53 -0.6070185730630229 1103 54-55 -0.29813354438512363 1103 56-57 -0.40529862534258854 1103 58-59 -0.27767256117586214 1103 60-61 -0.10298472742610443 1103 62-63 -0.07010005013043497 1103 64-65 -0.5379615536506464 1103 66-67 -0.8752178234060182 1103 68-69 -0.6956404098373703 1103 70-71 -0.9978210127290517 1103 72-73 -1.1417121143518294 1103 74-75 -0.5748926542775159 1103 76-77 -0.5373642340623093 1103 78-79 -0.8221596473127484 1103 80-81 -0.9700128921467694 1103 82-83 -1.0222034106831437 1103 84-85 -1.1251854693029308 1103 86-87 -0.7831665913329537 1103 88-89 -1.2432925137786945 1103 90-91 -1.140607466344992 1103 92-93 -0.7910840142228217 1103 94-95 -0.4561911324629442 1103 96-97 -0.3031216181476779 1103 98-99 -0.7554679643216389 1103 100-101 -0.2958840848203401 1104 1 0.3421303483064051 1104 2 0.46449812528147305 1104 3 0.024500435032997814 1104 4 0.08060671815557185 1104 5 -0.13196727208444514 1104 6 0.03890901075183706 1104 7 0.2693730621630266 1104 8 0.16626162795257926 1104 9 0.4407863568399719 1104 10-11 0.5306711424574999 1104 12-13 0.21342354005929565 1104 14-15 0.1520886087716704 1104 16-17 0.1815578021762576 1104 18-19 0.4286048198721275 1104 20-21 0.4071533560988101 1104 22-23 0.17605765509842541 1104 24-25 0.464822282403901 1104 26-27 0.3344610760284681 1104 28-29 0.19447888719734152 1104 30-31 -0.09385671313501831 1104 32-33 0.1515177233478724 1104 34-35 0.06474062060430441 1104 36-37 -0.1342712274277531 1104 38-39 -0.053320494128804796 1104 40-41 -0.11188691903615933 1104 42-43 -0.2336276834899067 1104 44-45 -0.695122001892365 1104 46-47 -0.564812406053278 1104 48-49 -0.015568469716583877 1104 50-51 -0.22456632771476137 1104 52-53 -0.07101510910977993 1104 54-55 -0.0593805675503134 1104 56-57 -0.35615378879789006 1104 58-59 -0.19142012558373978 1104 60-61 0.3982709556221806 1104 62-63 0.8918181139743524 1104 64-65 0.43766087544656074 1104 66-67 0.5242842333162159 1104 68-69 0.3273082803553109 1104 70-71 0.4019408404859277 1104 72-73 0.4374175673974712 1104 74-75 1.0449168282944719 1104 76-77 0.5279312844481936 1104 78-79 1.0611051286127768 1104 80-81 0.4625915676930248 1104 82-83 1.0397580528370973 1104 84-85 0.8585772499003568 1104 86-87 0.7443501871905376 1104 88-89 0.8992708116164145 1104 90-91 1.1771236442850146 1104 92-93 1.1014684863184208 1104 94-95 1.4834494823887603 1104 96-97 1.6242652621294411 1104 98-99 1.8822241768344554 1104 100-101 2.0763393401634254 1105 1 -0.03137822738337803 1105 2 0.1359653139466488 1105 3 0.17550714644239207 1105 4 0.24312536095945347 1105 5 0.02029782858669904 1105 6 0.1365973030710066 1105 7 -0.00859114365353264 1105 8 0.07994544599881692 1105 9 0.4213512337974663 1105 10-11 0.21933631769985595 1105 12-13 0.08520765639038785 1105 14-15 -0.17924062314481404 1105 16-17 -0.08137564301389943 1105 18-19 0.14409232919352633 1105 20-21 0.39275179010328287 1105 22-23 0.20935836352497716 1105 24-25 0.5502762346783214 1105 26-27 0.3802524630530826 1105 28-29 0.5746988722831006 1105 30-31 0.5038781861938375 1105 32-33 0.6335274176058832 1105 34-35 0.3515368547579243 1105 36-37 0.9109841789853803 1105 38-39 0.5116962843946808 1105 40-41 0.7839697178020231 1105 42-43 0.587238274750213 1105 44-45 0.7007579384506641 1105 46-47 0.7549116804493252 1105 48-49 -0.5481467694928739 1105 50-51 -0.5915499220473563 1105 52-53 -0.7372157056795103 1105 54-55 -0.4806380246718689 1105 56-57 -0.9347565106472544 1105 58-59 -0.5550247117135001 1105 60-61 -0.4536119675694721 1105 62-63 -0.6313455698436954 1105 64-65 -0.7416129873424033 1105 66-67 -0.43854667645261713 1105 68-69 -0.2724773274000931 1105 70-71 -0.10926814534554197 1105 72-73 -0.09634921112602157 1105 74-75 0.060949639629296115 1105 76-77 -0.026925165961948494 1105 78-79 0.5246817505367147 1105 80-81 0.2383652440539521 1105 82-83 0.09186468967527972 1105 84-85 0.1773458927042384 1105 86-87 0.033500075333709134 1105 88-89 -0.3079206499794118 1105 90-91 -0.49968004699015367 1105 92-93 -0.581137777663681 1105 94-95 -0.5961552901690332 1105 96-97 0.4688541883039363 1105 98-99 -0.049822803031318585 1105 100-101 0.03695641697177621 1106 1 0.3412063136794288 1106 2 0.14653294679688855 1106 3 0.07405787108007189 1106 4 0.17398284888215443 1106 5 0.003087683659160234 1106 6 0.17766010500336904 1106 7 0.2124233491000922 1106 8 0.2623125957572725 1106 9 0.2338512337974663 1106 10-11 0.3594329360573454 1106 12-13 -0.1004807494067137 1106 14-15 0.07408063289383193 1106 16-17 0.23882363234842074 1106 18-19 0.479086290546185 1106 20-21 0.6690199060453139 1106 22-23 0.4764175422689334 1106 24-25 0.5718643989295273 1106 26-27 0.4250894195748245 1106 28-29 0.37965056310435585 1106 30-31 0.6950013745996344 1106 32-33 0.5000733113256928 1106 34-35 0.7644293668352162 1106 36-37 0.8007788649757188 1106 38-39 1.1249209220758374 1106 40-41 0.18599266466192432 1106 42-43 0.368186342383062 1106 44-45 0.09266615101105202 1106 46-47 0.2814817287585072 1106 48-49 0.6502227957245239 1106 50-51 0.5938365513826014 1106 52-53 0.8829533764460962 1106 54-55 1.0047182555213752 1106 56-57 0.7981843106087894 1106 58-59 1.196333983938679 1106 60-61 1.036122331947439 1106 62-63 0.9263235122819111 1106 64-65 1.560168413623778 1106 66-67 1.6313506665425592 1106 68-69 1.3612787112472517 1106 70-71 1.346498762867018 1106 72-73 1.4004804990189044 1106 74-75 1.3809979488080408 1106 76-77 1.007797056260273 1106 78-79 1.000678127348312 1106 80-81 1.04784592038245 1106 82-83 1.0043043032018488 1106 84-85 0.8468809168588258 1106 86-87 1.040746452145303 1106 88-89 0.727254470793536 1106 90-91 0.9328380689518774 1106 92-93 1.167050628133417 1106 94-95 1.60070461321261 1106 96-97 1.2754665071445146 1106 98-99 0.6077858926208535 1106 100-101 1.0004226005466563 1107 1 0.37949228880209773 1107 2 0.2223060838504125 1107 3 0.5180268314817624 1107 4 0.1654241361125557 1107 5 0.019805702602447184 1107 6 -0.08475002851255198 1107 7 -0.16935229850917466 1107 8 0.23327003042576422 1107 9 -0.01680493418153617 1107 10-11 0.16246842618630097 1107 12-13 -0.10655743547312113 1107 14-15 -0.029251559277788886 1107 16-17 -0.127061338551961 1107 18-19 0.2312259687385776 1107 20-21 0.45656412606129493 1107 22-23 0.37211536265008505 1107 24-25 0.358866567136765 1107 26-27 0.6101573186961247 1107 28-29 0.6992614269637656 1107 30-31 0.06933094209934865 1107 32-33 0.26727632399258283 1107 34-35 0.3097334864289607 1107 36-37 0.15764865842545106 1107 38-39 0.36159785919782905 1107 40-41 0.27563091640219994 1107 42-43 0.6608657900608037 1107 44-45 0.7531420154410355 1107 46-47 0.6877638239313981 1107 48-49 0.8211117606908616 1107 50-51 0.7114903229220246 1107 52-53 0.47461719020850524 1107 54-55 0.7767712054243745 1107 56-57 0.7824077500701563 1107 58-59 0.9937777817248303 1107 60-61 1.1191133167699832 1107 62-63 1.080979452028572 1107 64-65 1.3968111158946925 1107 66-67 1.5522396315088969 1107 68-69 1.0389290593015659 1107 70-71 1.049169081251133 1107 72-73 1.53037322981886 1107 74-75 1.7319907594893138 1107 76-77 1.5589781586224767 1107 78-79 1.4809645589356677 1107 80-81 1.384089216057447 1107 82-83 1.0375121087478938 1107 84-85 1.2347332505345072 1107 86-87 1.5184082118166522 1107 88-89 1.3718967165997675 1107 90-91 1.7407234963169351 1107 92-93 2.2833635346722723 1107 94-95 2.2815787430768104 1107 96-97 2.4646711174377955 1107 98-99 2.4708464883072665 1107 100-101 2.120016346980524 1108 1 -0.047387557324000795 1108 2 0.06975454210610366 1108 3 -0.05731728103852163 1108 4 -0.02283095795488066 1108 5 -0.03933957599345206 1108 6 -0.3638267851393451 1108 7 0.13581089196988216 1108 8 -0.07760332414537174 1108 9 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78-79 -1.6270916654412453 2316 80-81 -1.702004361088381 2316 82-83 -1.8005825095901038 2316 84-85 -2.1269183405372694 2316 86-87 -2.507133533481717 2316 88-89 -2.046011788638733 2316 90-91 -1.7664672370727992 2316 92-93 -1.7667438382697433 2316 94-95 -1.371866952244332 2316 96-97 -0.9503090348365575 2316 98-99 -1.2781285881552833 2316 100-101 -1.268771314892323 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 6.0 6 5.0 7 42.0 8 92.0 9 150.0 10 233.0 11 391.0 12 587.0 13 756.0 14 957.0 15 1454.0 16 1890.0 17 2605.0 18 2284.0 19 1439.0 20 1455.0 21 1503.0 22 1341.0 23 1423.0 24 1549.0 25 1726.0 26 1894.0 27 2282.0 28 2792.0 29 3348.0 30 4081.0 31 4899.0 32 6475.0 33 8190.0 34 11403.0 35 16705.0 36 23392.0 37 27763.0 38 15272.0 39 621.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.70518883545947 19.03257428234577 15.48569990329717 20.77653697889759 2 17.349756630575147 22.41382735671004 35.08691765173339 25.149498360981426 3 21.702592629383133 23.665441541670805 30.599649018244428 24.03231681070163 4 14.393658446133879 17.820057746814655 34.34809673915923 33.43818706789224 5 14.399268709510752 37.14015076242333 31.88333797013897 16.57724255792695 6 31.554584285288566 34.72202907188503 15.57100758253038 18.152379060296017 7 27.363994569716233 34.57236515347174 19.45763385318367 18.606006423628358 8 26.89513592265157 30.198337803383996 19.472202907188503 23.434323366775935 9 25.390549981788684 18.396741829740737 20.817191483725704 35.39551670474488 10-11 26.550776464355486 25.310420184762094 24.612761166848777 23.526042184033642 12-13 27.918173269228223 23.270552707911154 26.02018516311042 22.791088859750204 14-15 24.314095559749678 25.76272308863945 26.525280619847024 23.397900731763848 16-17 21.980398000066224 28.271249296380912 27.027912983013806 22.720439720539055 18-19 21.3757822588656 26.83387967285851 30.022515810734745 21.76782225754114 20-21 23.334326677924572 24.564749511605577 29.699678818582164 22.401244991887687 22-23 22.220787391145986 24.62236349789742 30.49766564021059 22.659183470746 24-25 23.66411708221582 25.538227211019503 29.883447567961323 20.91420813880335 26-27 22.92032966123302 24.73088372123825 31.540659653584193 20.808126963944545 28-29 21.01294994520031 26.95019022611909 30.74345466525832 21.293405163422282 30-31 21.989006986523624 25.211416840501972 30.460911890334756 22.338664282639648 32-33 22.494619383464123 25.59385450812887 30.59170226151452 21.31982384689249 34-35 23.32406211714844 25.391212211516173 29.454984934273696 21.829740737061687 36-37 22.787324923015795 24.84387934174365 30.696003443594584 21.672792291645973 38-39 22.278401377437834 25.415052481705903 30.14171716168339 22.164828979172878 40-41 21.76829752854229 25.82348811952001 30.162114910863284 22.246099441074406 42-43 22.378351884539853 26.234861255079366 28.30038714121547 23.086399719165314 44-45 22.57044468726201 25.395516704744875 28.620575477633192 23.413463130359922 46-47 23.799211946624286 26.68189795039899 28.053044601172143 21.465845501804576 48-49 23.865766034237275 25.64683288632827 28.294758451706898 22.192642627727558 50-51 22.1535710738055 26.81136386212377 28.540776795470347 22.494288268600375 52-53 23.209827489155987 27.64047548094434 26.242839641071487 22.906857388828183 54-55 22.39826495811397 26.501440349657297 26.881229098374227 24.21906559385451 56-57 23.457501407238173 26.423628356676932 26.716996125956094 23.401874110128805 58-59 24.481308565941525 25.785238899374193 26.897122611834046 22.836329922850236 60-61 23.286315022681368 26.595808085824974 28.591768484487268 21.52610840700639 62-63 21.981060229793712 26.74745869342075 28.255355782921093 23.01612529386444 64-65 21.97079566901758 27.551074467732857 27.484520380119864 22.9936094831297 66-67 22.719446375947815 26.41932386344823 26.846462037680872 24.014767722923082 68-69 22.071785702460183 26.243832985662724 27.422601900599318 24.26177941127777 70-71 23.682328399721865 26.68057349094401 26.65772656534552 22.97937154398861 72-73 23.400880765537565 25.611403595907422 27.93649216913347 23.05122346942154 74-75 22.232376411377107 28.25105128969239 26.072315486242175 23.444256812688323 76-77 22.353233336644482 26.62428396410715 26.490513559153673 24.5319691400947 78-79 22.742955531273797 26.76434555147181 26.89745372669779 23.595245190556604 80-81 23.390947319625177 27.13486308400384 26.143174067083873 23.33101552928711 82-83 22.86339644633408 26.144212875581957 26.998854297048325 23.993536381035636 84-85 22.634243577135628 26.4328310270954 26.762624707374332 24.17030068839464 86-87 22.901559551008244 26.206085891195656 26.302771431409553 24.589583126386543 88-89 22.384689248700372 26.369656633886297 25.708751365848816 25.536902751564515 90-91 23.018409296380067 25.558421946769016 26.231011997999783 25.19215675885113 92-93 23.153771266978815 25.706139857083254 26.811459830327756 24.328629045610178 94-95 22.858514618721234 25.516042515148506 26.812026091851266 24.813416774279 96-97 21.598622562166817 26.273964438263636 27.450084434290257 24.677328565279296 98-99 22.53469359372982 26.74295780477548 25.442630984665616 25.279717616829085 100-101 22.25905809101146 25.50572961515533 26.24991720209313 25.985295091740078 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 773.0 1 484.5 2 208.5 3 239.0 4 209.0 5 183.5 6 283.0 7 460.5 8 588.5 9 615.0 10 526.0 11 424.0 12 414.5 13 439.0 14 470.0 15 492.5 16 519.0 17 508.5 18 498.5 19 508.0 20 483.0 21 523.0 22 599.5 23 650.0 24 693.0 25 687.5 26 698.0 27 761.5 28 852.0 29 986.0 30 1092.5 31 1156.0 32 1240.0 33 1353.0 34 1508.0 35 1589.0 36 1588.5 37 1717.0 38 2058.5 39 2213.5 40 2230.5 41 2799.0 42 3511.5 43 3942.0 44 4051.0 45 4109.5 46 4436.5 47 6067.0 48 9596.5 49 9791.5 50 6935.0 51 5678.5 52 5325.0 53 5354.0 54 5445.0 55 5199.0 56 4623.0 57 4044.5 58 3801.0 59 3617.0 60 3368.5 61 3058.0 62 2713.0 63 2290.5 64 1726.5 65 1352.0 66 1081.0 67 940.0 68 747.0 69 487.5 70 392.0 71 335.5 72 325.0 73 248.5 74 158.5 75 124.5 76 82.0 77 53.0 78 26.0 79 14.5 80 9.0 81 4.5 82 2.0 83 0.5 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017880202642296613 2 0.0 3 0.0 4 6.622297274924672E-4 5 0.025826959372206217 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0019866891824774015 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.933445912387007E-4 28-29 3.311148637462336E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.622297274924672E-4 42-43 0.017549087778550382 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.003973378364954803 84-85 9.933445912387007E-4 86-87 0.0 88-89 0.0 90-91 0.014237939141088044 92-93 0.005297837819939737 94-95 0.0 96-97 0.0 98-99 0.002980033773716102 100-101 0.02317804046223635 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 151005.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.45425648157346 #Duplication Level Percentage of deduplicated Percentage of total 1 71.61251158183968 29.686434224032315 2 13.722483146426404 11.377106718320587 3 5.399533531422729 6.715009436773617 4 2.797213968497396 4.63825701135724 5 1.5543627591935845 3.221747624250853 6 1.0016294450301926 2.4913082348266613 7 0.6853254097575002 1.988675871659879 8 0.5175884213553149 1.716499453660475 9 0.3306814914214512 1.233733982318466 >10 2.0783411610594587 16.39283467434853 >50 0.2028818812102623 5.756763021092016 >100 0.07987475638199304 7.1891659216582235 >500 0.011182465893479026 3.438958974868382 >1k 0.006389980510559442 4.1535048508327534 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2129 1.4098870898314626 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1445 0.956921956226615 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1393 0.9224860103970066 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1305 0.8642097943776695 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 983 0.6509718221250952 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 972 0.643687295122678 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 846 0.5602463494586273 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 680 0.45031621469487765 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 609 0.40329790404291255 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 584 0.3867421608556008 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 519 0.3436972285685905 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 488 0.3231681070163239 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 485 0.3211814178338465 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 462 0.30595013410151983 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 409 0.27085195854441907 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 396 0.26224297208701697 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 389 0.2576073639945697 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 361 0.23906493162478065 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 353 0.2337670938048409 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 340 0.22515810734743882 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 326 0.21588689116254425 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 307 0.2033045263401874 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 285 0.18873547233535312 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 281 0.18608655342538327 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 280 0.1854243236978908 No Hit GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG 266 0.17615310751299626 No Hit GATAATACGTCTCAATGAAGTCACATAAGTGGGGATCATTCTTGTCAGTA 263 0.17416641833051885 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 258 0.17085526969305653 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 246 0.1629085129631469 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 215 0.14237939141088043 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 200 0.13244594549849342 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 197 0.13045925631601601 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 187 0.12383695904109135 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 187 0.12383695904109135 No Hit TCCTACGTCTATCTGTCTATGTCTTGTTATTTTGACCGAGATGATGTGGC 179 0.11853912122115162 No Hit GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC 178 0.11787689149365915 No Hit AGATGATGTGGCTCTGAAGAACTTTGCCAAATACTTTCTCCACCAATCTC 177 0.11721466176616668 No Hit GGTGAAATCCATTAAAGAACTGGGTGACCACGTGACCAACTTACGCAAGA 174 0.11522797258368928 No Hit CCCTGGGACACGGTGATGAGAGCTAAGCTGACTTCCCCAAAGCCACGTGA 171 0.11324128340121188 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 170 0.11257905367371941 No Hit GTTCCAGTAGTGACTGATTCACACTCTTTTCCAAGTGCAGTGCACACTCC 169 0.11191682394622694 No Hit ACCTGGAGTTGTATGCCTCCTACGTCTATCTGTCTATGTCTTGTTATTTT 159 0.10529452667130228 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 157 0.10397006721631734 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 6.622297274924672E-4 10-11 0.0 0.0 0.0 0.0 6.622297274924672E-4 12-13 0.0 0.0 0.0 0.0 0.0013244594549849343 14-15 0.0 0.0 0.0 0.0 0.003311148637462336 16-17 0.0 0.0 0.0 0.0 0.0049667229561935035 18-19 0.0 0.0 0.0 0.0 0.006622297274924672 20-21 0.0 0.0 0.0 0.001655574318731168 0.006622297274924672 22-23 0.0 0.0 0.0 0.003973378364954803 0.007284527002417138 24-25 0.0 0.0 0.0 0.005297837819939737 0.007946756729909606 26-27 0.0 0.0 0.0 0.007284527002417138 0.007946756729909606 28-29 0.0 0.0 0.0 0.016555743187311678 0.007946756729909606 30-31 0.0 0.0 0.0 0.03973378364954803 0.007946756729909606 32-33 0.0 0.0 0.0 0.06589185788550048 0.008608986457402074 34-35 0.0 0.0 0.0 0.0966855402139002 0.009933445912387007 36-37 0.0 0.0 0.0 0.13873712790967185 0.009933445912387007 38-39 0.0 0.0 0.0 0.20462898579517236 0.010595675639879475 40-41 0.0 0.0 0.0 0.26489189099698685 0.010595675639879475 42-43 0.0 0.0 0.0 0.3046256746465349 0.01125790536737194 44-45 0.0 0.0 0.0 0.33806827588490446 0.011920135094864408 46-47 0.0 0.0 0.0 0.37713982980696004 0.011920135094864408 48-49 0.0 0.0 0.0 0.4122380053640608 0.011920135094864408 50-51 0.0 0.0 0.0 0.43773384987252073 0.011920135094864408 52-53 0.0 0.0 0.0 0.48177212675076986 0.012251249958610641 54-55 0.0 0.0 0.0 0.5688553359160293 0.01291347968610311 56-57 0.0 0.0 0.0 0.6950100990033443 0.013244594549849343 58-59 0.0 0.0 0.0 0.8244760107281215 0.013244594549849343 60-61 0.0 0.0 0.0 0.9582464156816 0.013244594549849343 62-63 0.0 0.0 0.0 1.0499652329393068 0.013244594549849343 64-65 0.0 0.0 0.0 1.1840667527565312 0.013906824277341811 66-67 0.0 0.0 0.0 1.3814112115492865 0.014569054004834277 68-69 0.0 0.0 0.0 1.651269825502467 0.014569054004834277 70-71 0.0 0.0 0.0 1.9313930002317803 0.014900168868580511 72-73 0.0 0.0 0.0 2.2138339790073176 0.017217972914804147 74-75 0.0 0.0 0.0 2.4972683023740934 0.017217972914804147 76-77 0.0 0.0 0.0 2.7869938081520482 0.017549087778550382 78-79 0.0 0.0 0.0 3.0863216449786433 0.017880202642296613 80-81 0.0 0.0 0.0 3.3505513062481374 0.018211317506042844 82-83 0.0 0.0 0.0 3.716102115823979 0.019204662097281548 84-85 0.0 0.0 0.0 4.234959107314327 0.019204662097281548 86-87 0.0 0.0 0.0 4.800172179729149 0.019204662097281548 88-89 0.0 0.0 0.0 5.479288765272673 0.019204662097281548 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGG 25 0.005009257 57.010334 4 TGAGCGC 45 9.6055784E-4 42.215893 6 CTAGAGC 60 0.0039366866 31.67241 3 TGTCCAC 60 0.0039366866 31.67241 5 TTTGAGC 95 3.46671E-5 30.005438 4 CATGGGG 445 0.0 26.690231 4 TAGAGCT 95 0.0012203335 25.004532 4 ATGGGGG 205 2.6255293E-8 23.174932 5 GTACATG 800 0.0 22.566591 1 AGTACTT 1215 0.0 22.280611 12-13 TACATGG 800 0.0 21.972733 2 ACATGGG 810 0.0 21.701466 3 ATGGGGA 220 5.547372E-8 21.594824 5 TCTATGT 155 3.8721606E-5 21.455502 2 GAGTACT 1285 0.0 21.436476 12-13 GTACTTT 1290 0.0 21.169308 14-15 CTGTACA 115 0.0037046378 20.655918 4 ATGGGGC 115 0.0037117586 20.64908 7 TACTTTT 1300 0.0 20.641138 14-15 GTCTATG 170 7.884621E-5 19.56237 1 >>END_MODULE