FastQCFastQC Report
Thu 26 May 2016
SRR937809_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937809_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139992
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14751.053631636093491No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13740.9814846562660724No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13050.9321961254928852No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12190.8707640436596377No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10840.7743299617120978No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8390.5993199611406366No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7310.5221726955826047No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5180.3700211440653751No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC5030.3593062460712041No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG4730.3378764500828619No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC4700.33573347048402763No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT4630.3307331847534145No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA4540.3243042459569118No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4080.2914452254414538No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4040.2885879193096748No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT4040.2885879193096748No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA3970.28358763357906164No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC3880.277158694782559No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.225012857877593No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT2920.208583347619864No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG2900.2071546945539745No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA2710.19358249042802445No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG2570.1835819189667981No Hit
GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG2520.1800102863020744No Hit
GTTCCAGTAGTGACTGATTCACACTCTTTTCCAAGTGCAGTGCACACTCC2460.17572432710440597No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2430.17358134750557175No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG2400.17143836790673753No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC2230.15929481684667696No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC2200.15715183724784274No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC2090.1492942453854506No Hit
GATAATACGTCTCAATGAAGTCACATAAGTGGGGATCATTCTTGTCAGTA2080.14857991885250585No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1910.13643636779244528No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC1910.13643636779244528No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.13072175552888737No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.12786444939710842No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG1720.12286416366649522No Hit
CTCCATTGCATTCAGCCCGCTCTCCCAGTCATCACGGTCTGGTTTCTTTA1650.11786387793588204No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG1610.11500657180410309No Hit
CCCTGGGACACGGTGATGAGAGCTAAGCTGACTTCCCCAAAGCCACGTGA1590.1135779187382136No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG1580.11286359220526887No Hit
AGATGATGTGGCTCTGAAGAACTTTGCCAAATACTTTCTCCACCAATCTC1500.10714897994171095No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACT301.2972433E-463.3333364
GTCTTAG407.5109474E-659.3750041
GTCCTAT250.00501206857.01
GATTAGA250.00501206857.01
TTAATAC459.5960114E-442.222223
AGGCCCG608.253368E-539.5833369
GAGTAAC500.001610958438.01
ATATAGA500.001610958438.01
AACTGTT500.001610958438.07
TGAACTG600.0039391831.6666685
GTGTAGG600.0039391831.6666682
TGTAGGA650.005826738729.2307663
CTCAATC350.00838088827.14285980-81
CAATCCC350.00838088827.14285932-33
CATGGGC1056.8632056E-527.1428574
ATGGGGA1806.5920176E-926.3888875
AGAGGGT908.908087E-426.3888876
CCAAATG559.451472E-525.9090952-53
CATGGGG3950.025.2531664
GTACATG6800.024.4485281
Produced by FastQC (version 0.11.5)