##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937809_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 139992 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.737299274244243 33.0 31.0 34.0 30.0 34.0 2 31.846269786844964 33.0 31.0 34.0 30.0 34.0 3 31.824875707183267 33.0 31.0 34.0 30.0 34.0 4 35.446454083090465 37.0 35.0 37.0 33.0 37.0 5 35.40988770786902 37.0 35.0 37.0 33.0 37.0 6 35.50996485513458 37.0 35.0 37.0 33.0 37.0 7 35.46326218641065 37.0 35.0 37.0 33.0 37.0 8 35.45051860106292 37.0 35.0 37.0 33.0 37.0 9 37.050395736899254 39.0 37.0 39.0 33.0 39.0 10-11 36.95599034230527 39.0 37.0 39.0 33.0 39.0 12-13 36.84250171438367 39.0 37.0 39.0 33.0 39.0 14-15 37.950450740042285 40.0 38.0 41.0 33.0 41.0 16-17 37.86296002628721 40.0 38.0 41.0 33.0 41.0 18-19 37.806121064060804 40.0 38.0 41.0 33.0 41.0 20-21 37.71769458254757 40.0 37.5 41.0 33.0 41.0 22-23 37.52884093376764 40.0 37.0 41.0 32.5 41.0 24-25 37.30904980284588 40.0 37.0 41.0 32.0 41.0 26-27 37.20534387679296 39.0 36.5 41.0 32.0 41.0 28-29 37.20658323332762 39.0 36.0 41.0 32.0 41.0 30-31 36.983834790559456 39.0 36.0 41.0 31.5 41.0 32-33 36.65538030744614 39.0 35.0 40.5 31.0 41.0 34-35 36.59655551745814 39.0 35.0 41.0 31.0 41.0 36-37 36.452382993313904 39.0 35.0 41.0 30.5 41.0 38-39 36.25424309960569 39.0 35.0 41.0 30.0 41.0 40-41 35.975459311960684 39.0 35.0 40.5 30.0 41.0 42-43 35.63412909309103 38.0 35.0 40.0 29.0 41.0 44-45 35.263875792902454 38.0 35.0 40.0 27.5 41.0 46-47 34.72572718441054 38.0 35.0 40.0 24.5 41.0 48-49 34.18819289673695 38.0 34.0 40.0 22.5 41.0 50-51 33.0979770272587 37.0 32.5 39.5 19.5 40.5 52-53 33.15321946968398 37.0 33.0 39.5 19.5 40.0 54-55 33.403980227441565 37.0 33.0 40.0 17.5 41.0 56-57 33.091351648665636 37.0 33.0 40.0 13.5 41.0 58-59 32.801431510372026 36.5 33.0 40.0 9.0 41.0 60-61 32.357259700554316 36.0 32.5 39.5 6.5 41.0 62-63 31.95501171495514 35.0 32.0 39.0 2.0 41.0 64-65 31.545134721984113 35.0 31.0 39.0 2.0 40.5 66-67 31.047902737299275 35.0 31.0 38.0 2.0 40.0 68-69 30.55294945425453 35.0 30.5 37.5 2.0 40.0 70-71 30.007468283901936 35.0 30.0 37.0 2.0 39.0 72-73 29.5512207840448 34.5 29.0 36.0 2.0 39.0 74-75 29.12608220469741 34.0 29.0 36.0 2.0 38.5 76-77 28.67614220812618 34.0 29.0 35.0 2.0 37.0 78-79 28.151558660494885 34.0 27.5 35.0 2.0 37.0 80-81 27.65487313560775 34.0 26.5 35.0 2.0 36.0 82-83 27.15713755071718 33.0 25.5 35.0 2.0 36.0 84-85 26.709611977827304 33.0 25.0 35.0 2.0 35.0 86-87 26.339730127435853 33.0 24.5 35.0 2.0 35.0 88-89 25.83097962740728 32.5 23.0 35.0 2.0 35.0 90-91 25.36085990628036 32.0 20.0 35.0 2.0 35.0 92-93 24.832322561289217 32.0 18.0 34.5 2.0 35.0 94-95 24.225462883593348 31.5 9.0 34.0 2.0 35.0 96-97 23.50358591919538 31.0 2.0 34.0 2.0 35.0 98-99 22.613706497514144 31.0 2.0 34.0 2.0 35.0 100-101 20.434171238356477 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5372035776052364 1101 2 0.5957969525964373 1101 3 0.754232036956541 1101 4 0.5887951338738731 1101 5 0.6572935340072803 1101 6 0.5954822804120781 1101 7 0.705697675934573 1101 8 0.9227616562728045 1101 9 1.3172532930662584 1101 10-11 0.8754122025254247 1101 12-13 1.0460327895394883 1101 14-15 1.2420586840792467 1101 16-17 1.3286426754872025 1101 18-19 1.2250629926552605 1101 20-21 1.202999664088665 1101 22-23 1.0566655452055542 1101 24-25 1.4091751512451651 1101 26-27 1.258356230025349 1101 28-29 1.397682416183578 1101 30-31 1.2682595273923596 1101 32-33 0.9872478989848972 1101 34-35 1.3867624919947232 1101 36-37 1.282462583702845 1101 38-39 1.0991592944189392 1101 40-41 1.5229272167703343 1101 42-43 1.3888177635593593 1101 44-45 1.6089849631530129 1101 46-47 1.1537847682028612 1101 48-49 1.6046956811215622 1101 50-51 1.7308264477293491 1101 52-53 1.6499239130516443 1101 54-55 1.5043712846172568 1101 56-57 1.241633763869963 1101 58-59 1.5423665330033742 1101 60-61 1.3908019656907555 1101 62-63 1.547330643519686 1101 64-65 1.3056200270565625 1101 66-67 1.352333838428283 1101 68-69 0.9365793918184728 1101 70-71 1.1768016506264054 1101 72-73 1.2471691263551712 1101 74-75 1.2212318777542137 1101 76-77 0.9812198918735966 1101 78-79 0.6452957679101736 1101 80-81 0.5616877922552135 1101 82-83 0.2723047687536102 1101 84-85 1.2353727640273 1101 86-87 0.7559279555857863 1101 88-89 1.0050897953925677 1101 90-91 0.9185424380206051 1101 92-93 1.2855968081639837 1101 94-95 1.3015592091189028 1101 96-97 1.5629689448541875 1101 98-99 1.614525301419949 1101 100-101 3.1549248124239107 1102 1 0.4846068632438154 1102 2 0.3936683534090122 1102 3 0.3383161210406307 1102 4 0.13913959598303904 1102 5 0.4150218505591212 1102 6 0.04447539116988963 1102 7 0.14594674853658773 1102 8 0.05529124468474578 1102 9 -0.03277320401906536 1102 10-11 0.058507062090875195 1102 12-13 0.20896778335683308 1102 14-15 0.290482107502676 1102 16-17 0.5299984430194371 1102 18-19 0.41820466373810206 1102 20-21 0.4189509094516808 1102 22-23 0.3624198289010181 1102 24-25 0.4771622559969728 1102 26-27 0.2970640554978843 1102 28-29 -0.011807956542092768 1102 30-31 0.5427914122183637 1102 32-33 0.4603975191933358 1102 34-35 -0.16852809270762492 1102 36-37 -0.4452798646278424 1102 38-39 0.13707220733185466 1102 40-41 -0.3069835763757567 1102 42-43 -0.30377188491263496 1102 44-45 -0.3138201949462669 1102 46-47 0.019223736583008133 1102 48-49 0.1259375111869261 1102 50-51 -0.09815694743052461 1102 52-53 0.13837560003274518 1102 54-55 0.12742816502708365 1102 56-57 -0.07328409516554046 1102 58-59 -0.1923976429372729 1102 60-61 0.42416621229030227 1102 62-63 0.10138469757373514 1102 64-65 0.12203938759357413 1102 66-67 0.5974760394528396 1102 68-69 0.5415961733058445 1102 70-71 1.1206057488422658 1102 72-73 0.34408221591531785 1102 74-75 0.6451631619796174 1102 76-77 0.7111041879343638 1102 78-79 1.195176531026231 1102 80-81 1.5867937805964942 1102 82-83 1.60285590812828 1102 84-85 1.2885877437128705 1102 86-87 1.3378039492264868 1102 88-89 1.345268209100393 1102 90-91 1.8254051831186437 1102 92-93 2.228937207386739 1102 94-95 1.825428666811888 1102 96-97 2.512889453598227 1102 98-99 2.904668385680683 1102 100-101 3.414596248565932 1103 1 0.24323707589225663 1103 2 0.14023928225003246 1103 3 -0.1178654126056955 1103 4 0.1856737025834292 1103 5 -0.1097411062667959 1103 6 0.18255879354492777 1103 7 0.19703760741534637 1103 8 -0.29507237494151184 1103 9 -0.04285329239244362 1103 10-11 0.0921994122703822 1103 12-13 0.23536472711085565 1103 14-15 0.3738154408360046 1103 16-17 0.13027000704411051 1103 18-19 0.10491643764954262 1103 20-21 0.32519227923903316 1103 22-23 0.47049310515987486 1103 24-25 -0.11323634475787969 1103 26-27 0.08577343596368081 1103 28-29 -0.15384974978520205 1103 30-31 -0.1106422617206988 1103 32-33 0.10154177032102041 1103 34-35 0.08289876911691607 1103 36-37 -0.037864785920298516 1103 38-39 0.2888718943428117 1103 40-41 0.8635724394578048 1103 42-43 0.5258562104569648 1103 44-45 0.3908654351743266 1103 46-47 0.7024143837338883 1103 48-49 0.8899069487271944 1103 50-51 1.1294275135839342 1103 52-53 1.0479539853774895 1103 54-55 1.3882216843127253 1103 56-57 1.2516261506229185 1103 58-59 1.3043573971069193 1103 60-61 1.2927338271296662 1103 62-63 1.1929339933483831 1103 64-65 1.3472073890664618 1103 66-67 1.8374880066810455 1103 68-69 1.8303745954728363 1103 70-71 1.707207056031809 1103 72-73 1.8211603711210635 1103 74-75 1.6787404426571442 1103 76-77 1.4283554076710843 1103 78-79 1.2977034572896962 1103 80-81 1.4651423031040878 1103 82-83 1.0491414646043857 1103 84-85 0.772201846631031 1103 86-87 1.0169671638080935 1103 88-89 0.6511689731618873 1103 90-91 0.8516410295699011 1103 92-93 1.1637848553661172 1103 94-95 1.8460491215665584 1103 96-97 1.6798322870696438 1103 98-99 2.052762834727904 1103 100-101 2.1677721668205585 1104 1 -0.11906439249252188 1104 2 -0.07302612580111045 1104 3 -0.3472630751768655 1104 4 -0.3352033326777857 1104 5 -0.24983700078283277 1104 6 -0.2171015800441296 1104 7 -0.38721970953600504 1104 8 -0.5565847113714355 1104 9 -0.4049050354750534 1104 10-11 -0.6381971515094591 1104 12-13 -0.43127696702558893 1104 14-15 -0.14568810526328235 1104 16-17 -0.3205091358986536 1104 18-19 0.1429995462301079 1104 20-21 0.43167514808330054 1104 22-23 -0.2709413169757724 1104 24-25 -0.3104194433494314 1104 26-27 0.016574554733033153 1104 28-29 0.15096495404058885 1104 30-31 0.10439619597583771 1104 32-33 -0.027990743913321126 1104 34-35 -0.13633507365603492 1104 36-37 0.16318905485484692 1104 38-39 -0.20808145697075275 1104 40-41 -0.15603099676234677 1104 42-43 -0.4980425009604801 1104 44-45 -0.5621862080181614 1104 46-47 -0.6602367000739235 1104 48-49 -0.007006446538014188 1104 50-51 -0.5537959406164461 1104 52-53 -0.9296956000785102 1104 54-55 -0.33363427413298297 1104 56-57 0.16826784475936307 1104 58-59 0.40751891943235563 1104 60-61 0.3571379825586902 1104 62-63 0.8070584564389804 1104 64-65 -0.06159293058192361 1104 66-67 -0.3158183563196566 1104 68-69 -0.22721205920701593 1104 70-71 0.12387372269847674 1104 72-73 0.23185860306592687 1104 74-75 0.17367101902314275 1104 76-77 -0.2907036273903465 1104 78-79 -0.40849472471010273 1104 80-81 0.1426969929452646 1104 82-83 -0.031270082693584555 1104 84-85 -0.7461779356085039 1104 86-87 -0.20746096524994684 1104 88-89 -0.008855000467121954 1104 90-91 0.35293960114116985 1104 92-93 1.0248626697702719 1104 94-95 1.5163617776448746 1104 96-97 1.9035861578118265 1104 98-99 1.613471055684851 1104 100-101 2.8490977087244644 1105 1 0.8349904949573705 1105 2 0.9352994986207221 1105 3 0.7996687780454614 1105 4 0.6510748048491948 1105 5 0.87566407846478 1105 6 0.9042366869925687 1105 7 0.6280439352373506 1105 8 0.7520801051564661 1105 9 0.9194859605163117 1105 10-11 0.6940569342136342 1105 12-13 0.6485102670169667 1105 14-15 0.4426560205461456 1105 16-17 0.9582451040026712 1105 18-19 1.1518647944570617 1105 20-21 1.329223714225762 1105 22-23 0.7410074958518535 1105 24-25 0.9975515711433189 1105 26-27 1.1435403271659652 1105 28-29 1.2730741122274551 1105 30-31 1.2211091161115135 1105 32-33 1.1482349730154482 1105 34-35 1.0963198754651557 1105 36-37 1.207746563334645 1105 38-39 1.757845154191763 1105 40-41 1.9646134682391931 1105 42-43 1.6419513319103203 1105 44-45 1.3001326325615494 1105 46-47 0.9195505339686321 1105 48-49 0.9633913332954762 1105 50-51 0.5327751355475954 1105 52-53 0.8566904622094995 1105 54-55 1.1699118251577971 1105 56-57 0.8892052483979711 1105 58-59 0.8129305753065452 1105 60-61 0.3639218246801903 1105 62-63 0.5941383207621413 1105 64-65 0.7779075319527635 1105 66-67 0.4971634506492002 1105 68-69 0.6566146444624259 1105 70-71 1.207207056031809 1105 72-73 0.7134343045768681 1105 74-75 0.784833522877598 1105 76-77 0.9424909455359547 1105 78-79 0.925994945348485 1105 80-81 0.8828295862230924 1105 82-83 1.042966735067747 1105 84-85 0.7260699829853898 1105 86-87 0.36785510012162703 1105 88-89 0.5659985302143475 1105 90-91 0.9257888148321989 1105 92-93 1.048914473655561 1105 94-95 1.4660225855387985 1105 96-97 1.2935799906826233 1105 98-99 1.4494254189287616 1105 100-101 1.118986700398839 1106 1 0.07133386173552125 1106 2 0.14228574908523228 1106 3 -0.012111229386722044 1106 4 0.005404050482788136 1106 5 -0.5100661333523888 1106 6 -0.448234513304719 1106 7 -0.44832172808483506 1106 8 -0.33636285285170686 1106 9 -0.03629256047960183 1106 10-11 -0.6526543255410999 1106 12-13 -0.29807092956423986 1106 14-15 -0.6796033625828031 1106 16-17 -0.4850845132399897 1106 18-19 -0.2733488807566218 1106 20-21 0.003415464504470833 1106 22-23 0.42727654081654975 1106 24-25 0.41180277887279004 1106 26-27 -0.14436379769468033 1106 28-29 0.29462743812860026 1106 30-31 0.3381659722988104 1106 32-33 0.6946861064231058 1106 34-35 0.40085350608574544 1106 36-37 0.02807357931384047 1106 38-39 0.4362175064771563 1106 40-41 0.7686825875256957 1106 42-43 0.852839477581071 1106 44-45 0.7198650740331161 1106 46-47 0.652456516874615 1106 48-49 1.4572597836856644 1106 50-51 1.6476952395981357 1106 52-53 1.3794515175792483 1106 54-55 1.3323049875509554 1106 56-57 1.4538652261014278 1106 58-59 1.4808049714418203 1106 60-61 1.506619706508502 1106 62-63 1.6868547830438203 1106 64-65 1.9154028868394235 1106 66-67 1.8981296342240803 1106 68-69 2.3047937600328403 1106 70-71 1.7478053466300985 1106 72-73 2.207581461767507 1106 74-75 2.3465206280429634 1106 76-77 2.093457129110835 1106 78-79 2.589595837210247 1106 80-81 3.421384026951447 1106 82-83 2.8700756165244563 1106 84-85 2.8039220825766193 1106 86-87 3.084410284068113 1106 88-89 2.342754383057155 1106 90-91 3.112986882465048 1106 92-93 3.110787383354559 1106 94-95 3.5370928196525107 1106 96-97 3.9040407859260284 1106 98-99 3.561279748174396 1106 100-101 4.3530075105066075 1107 1 -0.01871257476760846 1107 2 -0.36414133214499955 1107 3 -0.2688579821964687 1107 4 -0.22893204212101637 1107 5 -0.340471948010169 1107 6 -0.4259584516585804 1107 7 -0.12346810401860608 1107 8 -0.34309278084147365 1107 9 -0.07229767522487407 1107 10-11 -0.24078273256714766 1107 12-13 -0.142172147681201 1107 14-15 0.21042961406434557 1107 16-17 -0.6090834364515132 1107 18-19 -0.197872738351343 1107 20-21 -0.26763238528353384 1107 22-23 -0.17622975166612065 1107 24-25 0.07934433617812431 1107 26-27 -0.1335622632710951 1107 28-29 -0.2232741703241885 1107 30-31 0.16342339204441458 1107 32-33 -0.2603579746052276 1107 34-35 0.06573674183511713 1107 36-37 0.12141768053195534 1107 38-39 0.2805542720737648 1107 40-41 0.4701766315394309 1107 42-43 0.3955745203161172 1107 44-45 1.0506746843702928 1107 46-47 0.4503904289817555 1107 48-49 0.11453820080659938 1107 50-51 -0.5242544205405011 1107 52-53 -0.8751080086338163 1107 54-55 -0.912765340196529 1107 56-57 -0.9161125651362099 1107 58-59 -0.6429920539300085 1107 60-61 -0.4181733482058121 1107 62-63 -0.6702688457333537 1107 64-65 -0.7599790365739949 1107 66-67 -0.6408029991967403 1107 68-69 -0.8766787482113187 1107 70-71 -0.4758638101099244 1107 72-73 -0.3038257661751551 1107 74-75 -0.665525943058725 1107 76-77 -0.39691907386379555 1107 78-79 0.11574160745050577 1107 80-81 0.16221450006538163 1107 82-83 -0.6797595002397934 1107 84-85 -0.20317799145258775 1107 86-87 0.9605459594163896 1107 88-89 0.24210830997013844 1107 90-91 0.8417707844773012 1107 92-93 0.9250413708368939 1107 94-95 1.4225157956266692 1107 96-97 1.44943415021703 1107 98-99 1.9810926561762834 1107 100-101 2.1953589473462323 1108 1 0.22005181045346944 1108 2 -0.043184336384854305 1108 3 -0.1343334516089456 1108 4 0.15241259749133462 1108 5 0.06685694357068428 1108 6 0.45005608498588145 1108 7 0.023473143710042166 1108 8 -0.19106370755255853 1108 9 -0.0320190562060958 1108 10-11 0.24349952061274394 1108 12-13 0.32030513881183253 1108 14-15 0.38535390237446876 1108 16-17 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-0.9743148102203634 2316 68-69 -1.0099070946680122 2316 70-71 -1.7787929439681918 2316 72-73 -1.0472903331042893 2316 74-75 -0.662470824948489 2316 76-77 -1.2763206486669425 2316 78-79 -1.496352880738474 2316 80-81 -1.4180689645015434 2316 82-83 -1.4448303663815274 2316 84-85 -1.8842488575943221 2316 86-87 -2.034666639008808 2316 88-89 -2.129450745147974 2316 90-91 -1.503457561979392 2316 92-93 -2.680186975619801 2316 94-95 -1.5080353854756936 2316 96-97 -1.7014634875782448 2316 98-99 -1.4270963202016738 2316 100-101 -1.0151292416301452 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 871.0 3 161.0 4 150.0 5 150.0 6 157.0 7 180.0 8 222.0 9 293.0 10 347.0 11 413.0 12 555.0 13 819.0 14 965.0 15 1252.0 16 1641.0 17 2118.0 18 1886.0 19 1311.0 20 1415.0 21 1447.0 22 1488.0 23 1575.0 24 1673.0 25 1767.0 26 2052.0 27 2285.0 28 2703.0 29 3179.0 30 3819.0 31 4812.0 32 6011.0 33 7906.0 34 10933.0 35 15712.0 36 20464.0 37 23048.0 38 13287.0 39 925.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.373720295451456 18.068819268822132 15.114304750578508 21.443155685147904 2 16.962143189713835 21.483471222377425 36.082518534436446 25.471867053472298 3 20.820410706739345 23.497811808355955 30.820195827036162 24.861581657868538 4 13.768333906267905 17.218832359344564 35.312822275696114 33.70001145869142 5 14.543319153811998 36.02386169953738 32.62292499176442 16.80989415488621 6 31.07649576932438 33.954930061419965 16.513236073131274 18.455338096124386 7 26.75002863032524 33.80096197892808 20.588782638570773 18.860226752175905 8 26.018140037654536 29.93607319011246 20.21604827798498 23.82973849424802 9 25.017897027661647 18.0008304220835 21.79571616745891 35.185556382795944 10-11 26.09185857559076 24.830521214377242 25.20706119848525 23.87055901154675 12-13 27.29628357097864 23.012225848937753 26.892214968791155 22.799275611292448 14-15 24.249658203474514 25.43646163647166 26.91101837469489 23.40286178535894 16-17 21.80280520670415 28.297227362039163 27.246438017386698 22.65352941386999 18-19 20.95889822622439 26.729037522726944 30.264777884353837 22.04728636669482 20-21 22.92230285853337 24.86652066245831 29.357939336682843 22.853237142325476 22-23 21.862252565588904 24.545207322484146 30.567005882605525 23.025534229321426 24-25 23.41162494453748 25.285900353528845 29.330728383929465 21.971746318004207 26-27 22.124201017829918 24.77900493167942 31.40420445354272 21.692589596947943 28-29 20.765725617373437 26.613546957608037 30.730682359173713 21.890045065844816 30-31 21.45288468765771 25.27193268167322 30.385233491655008 22.889949139014064 32-33 22.054275774192163 25.508611679814454 30.31769438916489 22.119418156828495 34-35 22.98973167789895 25.57596017222435 28.97248776856369 22.461820381313014 36-37 22.66528297160443 25.056451180017536 30.385585714541325 21.892680133836713 38-39 21.93097969612172 25.683654554953588 29.79774846665283 22.587617282271857 40-41 21.646592381075067 26.09442428666509 30.080654972769103 22.178328359490738 42-43 22.12400781579908 26.143203761890106 28.006971234710164 23.72581718760065 44-45 21.964556672106273 25.664204529187778 28.509619513870994 23.86161928483495 46-47 23.035354493302364 26.471672386705652 28.371631946805426 22.121341173186558 48-49 23.032237974737914 25.403824747397017 28.519529977701502 23.04440730016357 50-51 21.425477766283784 26.844755935612962 28.82669406134451 22.903072236758742 52-53 22.695918929851125 26.9341322938256 26.837125889971112 23.532822886352164 54-55 21.940325749685954 25.776885443620106 27.754216815727034 24.528571990966906 56-57 22.911739766657245 25.781118628422593 27.24943918312147 24.057702421798695 58-59 23.684370583710486 25.06753009785156 27.750845244271122 23.497254074166833 60-61 23.09506089794049 25.630447539036222 29.321893203188733 21.952598359834553 62-63 21.56577873838799 25.968323719279162 28.996392940469207 23.469504601863648 64-65 21.4830318968139 26.7453800604662 28.380114134421007 23.391473908298895 66-67 22.198885064298445 25.92762220997717 27.284437415467405 24.58905531025698 68-69 21.389159247702747 25.7253170354679 28.333166347007126 24.552357369822232 70-71 23.20215251514382 25.740732127075677 27.360127662467303 23.696987695313197 72-73 22.659825316573457 24.947312301191154 28.970541404122702 23.42232097811269 74-75 21.87383712609131 27.058823529411764 27.22770860168885 23.839630742808072 76-77 21.782341215164323 25.78672173331091 27.327776564943683 25.103160486581082 78-79 22.515300096632192 25.48477148276726 28.074872051823483 23.925056368777067 80-81 22.9416858709188 25.7532599516196 27.29663770522308 24.008416472238526 82-83 22.27844477446367 25.04177924099554 28.491474172019537 24.18830181252125 84-85 22.348891465212475 25.355317961269268 27.689753388581163 24.606037184937094 86-87 22.604261714347057 25.17417207879341 27.575546132789437 24.646020074070098 88-89 22.117686634196616 25.29121054119533 26.896223477921676 25.69487934668637 90-91 22.343219914399004 24.569847836356086 27.71368041340414 25.373251835840765 92-93 22.97582114013147 24.437931861203253 28.25817025037662 24.32807674828866 94-95 22.536052961173734 24.24619788870997 28.19788870996601 25.019860440150293 96-97 21.394323662854774 24.978889063819434 28.683321644792397 24.9434656285334 98-99 22.354498774444025 25.35022274703451 26.78331812570447 25.51196035281699 100-101 22.15301058687571 23.596675456266077 28.15879955777701 26.091514399081206 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 599.0 1 393.0 2 217.5 3 254.5 4 200.5 5 175.0 6 274.0 7 444.5 8 597.0 9 626.0 10 530.5 11 400.5 12 365.0 13 392.0 14 397.5 15 402.0 16 407.0 17 427.0 18 430.5 19 440.5 20 491.0 21 531.5 22 566.5 23 627.0 24 669.0 25 653.0 26 661.5 27 767.0 28 859.0 29 932.5 30 1044.5 31 1150.5 32 1257.5 33 1317.5 34 1452.5 35 1484.5 36 1433.0 37 1569.5 38 1873.0 39 2044.5 40 2110.5 41 2583.5 42 3253.0 43 3637.0 44 3757.5 45 3963.5 46 4242.0 47 5722.0 48 8904.0 49 8987.5 50 6296.5 51 5104.0 52 4865.0 53 5012.5 54 5150.0 55 4848.5 56 4239.5 57 3763.5 58 3553.5 59 3280.0 60 2983.0 61 2776.0 62 2484.5 63 2045.5 64 1593.0 65 1229.0 66 953.5 67 860.0 68 688.0 69 430.0 70 350.0 71 321.5 72 288.0 73 214.0 74 137.5 75 122.0 76 84.0 77 42.0 78 24.0 79 11.5 80 6.5 81 5.0 82 1.5 83 1.0 84 1.5 85 2.0 86 1.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 1.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2921595519743985 2 0.27644436824961427 3 0.2707297559860563 4 0.2571575518601063 5 0.25287159266243786 6 0.21286930681753244 7 0.20001142922452714 8 0.21501228641636663 9 0.21715526601520088 10-11 0.2135836333504772 12-13 0.20572604148808502 14-15 0.20644036802102977 16-17 0.20536887822161265 18-19 0.2071546945539745 20-21 0.19286816389507971 22-23 0.18429624549974286 24-25 0.18429624549974286 26-27 0.20215440882336133 28-29 0.21965540888050747 30-31 0.21251214355106005 32-33 0.21286930681753244 34-35 0.2075118578204469 36-37 0.19251100062860735 38-39 0.189296531230356 40-41 0.18643922509857708 42-43 0.19643979655980343 44-45 0.19715412309274813 46-47 0.19893993942510999 48-49 0.21179781701811534 50-51 0.21822675581461798 52-53 0.22179838847934166 54-55 0.20251157208983372 56-57 0.17179553117320992 58-59 0.17108120464026516 60-61 0.17858163323618492 62-63 0.19001085776330076 64-65 0.17465283730498884 66-67 0.18072461283501914 68-69 0.18572489856563232 70-71 0.17751014343676783 72-73 0.1810817761014915 74-75 0.1800102863020744 76-77 0.20179724555688897 78-79 0.2050117149551403 80-81 0.19001085776330076 82-83 0.19251100062860735 84-85 0.18429624549974286 86-87 0.18536773529915995 88-89 0.1953683067603863 90-91 0.1957254700268587 92-93 0.18751071489799417 94-95 0.1907251842962455 96-97 0.18143893936796388 98-99 0.18536773529915995 100-101 0.1739385107720441 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 139992.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.070375450025715 #Duplication Level Percentage of deduplicated Percentage of total 1 73.56998135991572 32.42256700382879 2 13.195558797309346 11.630664609406253 3 5.168976416241187 6.833961940682324 4 2.321095712780614 4.0916623807074695 5 1.4166464057054866 3.1216069489685125 6 0.925520706702326 2.4472827018686782 7 0.575411297511954 1.775101434367678 8 0.38576870086716913 1.3600777187267843 9 0.2544776724207797 1.0093433910509173 >10 1.936947888807845 16.593805360306305 >50 0.1572250587567874 4.620978341619521 >100 0.07780209093119378 7.629721698382765 >500 0.006483507577599482 1.8508200468598204 >1k 0.008104384471999352 4.612406423224185 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1475 1.053631636093491 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1374 0.9814846562660724 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1305 0.9321961254928852 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1219 0.8707640436596377 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1084 0.7743299617120978 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 839 0.5993199611406366 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 731 0.5221726955826047 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 518 0.3700211440653751 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 503 0.3593062460712041 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 473 0.3378764500828619 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 470 0.33573347048402763 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 463 0.3307331847534145 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 454 0.3243042459569118 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 408 0.2914452254414538 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 404 0.2885879193096748 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 404 0.2885879193096748 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 397 0.28358763357906164 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 388 0.277158694782559 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 315 0.225012857877593 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 292 0.208583347619864 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 290 0.2071546945539745 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 271 0.19358249042802445 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 257 0.1835819189667981 No Hit GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG 252 0.1800102863020744 No Hit GTTCCAGTAGTGACTGATTCACACTCTTTTCCAAGTGCAGTGCACACTCC 246 0.17572432710440597 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 243 0.17358134750557175 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 240 0.17143836790673753 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 223 0.15929481684667696 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 220 0.15715183724784274 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 209 0.1492942453854506 No Hit GATAATACGTCTCAATGAAGTCACATAAGTGGGGATCATTCTTGTCAGTA 208 0.14857991885250585 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 191 0.13643636779244528 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 191 0.13643636779244528 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 183 0.13072175552888737 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 179 0.12786444939710842 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 172 0.12286416366649522 No Hit CTCCATTGCATTCAGCCCGCTCTCCCAGTCATCACGGTCTGGTTTCTTTA 165 0.11786387793588204 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 161 0.11500657180410309 No Hit CCCTGGGACACGGTGATGAGAGCTAAGCTGACTTCCCCAAAGCCACGTGA 159 0.1135779187382136 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 158 0.11286359220526887 No Hit AGATGATGTGGCTCTGAAGAACTTTGCCAAATACTTTCTCCACCAATCTC 150 0.10714897994171095 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 7.143265329447397E-4 9 0.0 0.0 0.0 0.0 7.143265329447397E-4 10-11 0.0 0.0 0.0 0.0 7.143265329447397E-4 12-13 0.0 0.0 0.0 0.0 7.143265329447397E-4 14-15 0.0 0.0 0.0 0.0 0.0014286530658894794 16-17 0.0 0.0 0.0 3.5716326647236985E-4 0.002142979598834219 18-19 0.0 0.0 0.0 7.143265329447397E-4 0.002142979598834219 20-21 0.0 0.0 0.0 0.0010714897994171095 0.002142979598834219 22-23 0.0 0.0 0.0 0.0014286530658894794 0.002500142865306589 24-25 0.0 0.0 0.0 0.003928795931196068 0.003214469398251329 26-27 0.0 0.0 0.0 0.009643408194753986 0.004285959197668438 28-29 0.0 0.0 0.0 0.017501000057146124 0.004285959197668438 30-31 0.0 0.0 0.0 0.035716326647236986 0.004643122464140808 32-33 0.0 0.0 0.0 0.06964683696211212 0.005714612263557918 34-35 0.0 0.0 0.0 0.10322018401051489 0.005714612263557918 36-37 0.0 0.0 0.0 0.14393679638836504 0.005714612263557918 38-39 0.0 0.0 0.0 0.20572604148808504 0.005714612263557918 40-41 0.0 0.0 0.0 0.2571575518601063 0.005714612263557918 42-43 0.0 0.0 0.0 0.29537402137264984 0.005714612263557918 44-45 0.0 0.0 0.0 0.32251842962455 0.006071775530030288 46-47 0.0 0.0 0.0 0.3618063889365106 0.009643408194753986 48-49 0.0 0.0 0.0 0.39859420538316476 0.012143551060060575 50-51 0.0 0.0 0.0 0.4318103891650952 0.012143551060060575 52-53 0.0 0.0 0.0 0.48538487913595063 0.012857877593005315 54-55 0.0 0.0 0.0 0.5964626550088576 0.013572204125950055 56-57 0.0 0.0 0.0 0.7293273901365793 0.013929367392422424 58-59 0.0 0.0 0.0 0.847905594605406 0.014286530658894793 60-61 0.0 0.0 0.0 0.9836276358649066 0.014286530658894793 62-63 0.0 0.0 0.0 1.0739899422824162 0.014286530658894793 64-65 0.0 0.0 0.0 1.1957826161494944 0.015000857191839533 66-67 0.0 0.0 0.0 1.3922224127092977 0.016072346991256644 68-69 0.0 0.0 0.0 1.6540230870335448 0.016429510257729013 70-71 0.0 0.0 0.0 1.9526115778044462 0.016429510257729013 72-73 0.0 0.0 0.0 2.240128007314704 0.02000114292245271 74-75 0.0 0.0 0.0 2.554074518543917 0.02000114292245271 76-77 0.0 0.0 0.0 2.8330190296588373 0.02000114292245271 78-79 0.0 0.0 0.0 3.1194639693696784 0.02000114292245271 80-81 0.0 0.0 0.0 3.4187667866735243 0.02107263272186982 82-83 0.0 0.0 0.0 3.7559289102234414 0.02285844905423167 84-85 0.0 0.0 0.0 4.29667409566261 0.02285844905423167 86-87 0.0 0.0 0.0 4.923495628321618 0.02285844905423167 88-89 0.0 0.0 0.0 5.618892508143322 0.02285844905423167 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAATACT 30 1.2972433E-4 63.333336 4 GTCTTAG 40 7.5109474E-6 59.375004 1 GTCCTAT 25 0.005012068 57.0 1 GATTAGA 25 0.005012068 57.0 1 TTAATAC 45 9.5960114E-4 42.22222 3 AGGCCCG 60 8.253368E-5 39.583336 9 GAGTAAC 50 0.0016109584 38.0 1 ATATAGA 50 0.0016109584 38.0 1 AACTGTT 50 0.0016109584 38.0 7 TGAACTG 60 0.00393918 31.666668 5 GTGTAGG 60 0.00393918 31.666668 2 TGTAGGA 65 0.0058267387 29.230766 3 CTCAATC 35 0.008380888 27.142859 80-81 CAATCCC 35 0.008380888 27.142859 32-33 CATGGGC 105 6.8632056E-5 27.142857 4 ATGGGGA 180 6.5920176E-9 26.388887 5 AGAGGGT 90 8.908087E-4 26.388887 6 CCAAATG 55 9.451472E-5 25.90909 52-53 CATGGGG 395 0.0 25.253166 4 GTACATG 680 0.0 24.448528 1 >>END_MODULE