##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937809_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 139992 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.149629978855934 33.0 31.0 34.0 31.0 34.0 2 32.334469112520715 34.0 31.0 34.0 31.0 34.0 3 32.37027830161723 34.0 31.0 34.0 30.0 34.0 4 35.99952854448826 37.0 35.0 37.0 35.0 37.0 5 35.90862334990571 37.0 35.0 37.0 35.0 37.0 6 35.98954225955769 37.0 35.0 37.0 35.0 37.0 7 35.94146094062518 37.0 35.0 37.0 35.0 37.0 8 35.932124692839594 37.0 35.0 37.0 35.0 37.0 9 37.595612606434656 39.0 37.0 39.0 35.0 39.0 10-11 37.513029315960914 39.0 37.0 39.0 35.0 39.0 12-13 37.407241128064456 39.0 37.0 39.0 34.0 39.0 14-15 38.52415852334419 40.0 38.0 41.0 34.0 41.0 16-17 38.503846648379906 40.0 38.0 41.0 34.0 41.0 18-19 38.49828561632093 40.0 38.0 41.0 34.0 41.0 20-21 38.39211526372935 40.0 38.0 41.0 34.0 41.0 22-23 38.31512872164124 40.0 38.0 41.0 34.0 41.0 24-25 38.25347877021544 40.0 37.5 41.0 34.0 41.0 26-27 37.99177452997314 40.0 37.0 41.0 33.0 41.0 28-29 37.91516300931482 40.0 37.0 41.0 33.0 41.0 30-31 37.877914452254416 40.0 37.0 41.0 33.0 41.0 32-33 37.63475412880736 40.0 36.0 41.0 33.0 41.0 34-35 37.37776801531516 40.0 35.0 41.0 33.0 41.0 36-37 37.1256678953083 39.5 35.0 41.0 32.5 41.0 38-39 36.88934367678153 39.0 35.0 41.0 31.5 41.0 40-41 36.67675295731185 39.0 35.0 41.0 31.0 41.0 42-43 36.32241842390994 39.0 35.0 41.0 30.5 41.0 44-45 35.85840262300703 39.0 35.0 41.0 30.0 41.0 46-47 35.294434681981826 38.0 35.0 40.0 27.5 41.0 48-49 34.73119892565289 38.0 34.5 40.0 24.5 41.0 50-51 34.31011772101263 38.0 34.0 40.0 23.5 41.0 52-53 33.98382407566147 38.0 34.0 40.0 22.5 41.0 54-55 33.67053831647523 37.0 33.5 40.0 21.0 41.0 56-57 33.22465926624379 37.0 33.0 40.0 19.0 41.0 58-59 32.902601577232986 36.0 33.0 40.0 15.5 41.0 60-61 32.405501742956744 35.5 32.0 39.0 10.5 41.0 62-63 31.943732498999942 35.0 32.0 39.0 6.5 40.0 64-65 31.537702154408823 35.0 31.0 38.5 2.0 40.0 66-67 31.027815875192868 35.0 31.0 38.0 2.0 40.0 68-69 30.53723069889708 35.0 30.5 37.0 2.0 39.5 70-71 30.020101148637064 34.5 30.0 36.5 2.0 39.0 72-73 29.55326018629636 34.0 29.0 36.0 2.0 38.5 74-75 29.08644422538431 34.0 29.0 35.0 2.0 37.5 76-77 27.892247414137948 32.5 27.0 34.5 2.0 36.0 78-79 28.46810532030402 34.0 29.0 35.0 2.0 36.0 80-81 28.292020258300475 34.0 29.0 35.0 2.0 36.0 82-83 27.938767929595976 34.0 28.0 35.0 2.0 36.0 84-85 27.5669752557289 33.5 27.0 35.0 2.0 35.0 86-87 27.304978141608093 33.0 26.5 35.0 2.0 35.0 88-89 26.924202811589232 33.0 25.5 35.0 2.0 35.0 90-91 26.47113049317104 33.0 25.0 35.0 2.0 35.0 92-93 26.0674609977713 33.0 24.0 35.0 2.0 35.0 94-95 25.577825875764333 32.0 22.0 34.5 2.0 35.0 96-97 25.04594191096634 32.0 18.5 34.0 2.0 35.0 98-99 24.316257357563288 32.0 5.5 34.0 2.0 35.0 100-101 22.08992299559975 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.612153795713489 1101 2 0.6386385930540328 1101 3 0.5177801495547669 1101 4 0.35908373511087177 1101 5 0.44201587454210056 1101 6 0.38383287813363154 1101 7 0.12498265652556739 1101 8 0.31768299329885963 1101 9 0.7270704980953582 1101 10-11 0.6774551569706802 1101 12-13 0.4455743490213777 1101 14-15 0.8095874091507582 1101 16-17 0.8533053684581162 1101 18-19 0.9057783864521198 1101 20-21 1.0694413844390098 1101 22-23 0.9026472877891223 1101 24-25 0.5970712846714719 1101 26-27 0.7283681364172381 1101 28-29 1.1601712631058163 1101 30-31 1.2217532480503763 1101 32-33 1.199981140851122 1101 34-35 0.9376741821343444 1101 36-37 0.9063426593692014 1101 38-39 1.0420732353561064 1101 40-41 0.8896338470581142 1101 42-43 0.9742961060376558 1101 44-45 1.2856437750819012 1101 46-47 0.7102092148478292 1101 48-49 0.45187757336896084 1101 50-51 0.7125078425120179 1101 52-53 0.40384784873155866 1101 54-55 0.26982050328810203 1101 56-57 0.30820970094198685 1101 58-59 0.6871800044688428 1101 60-61 0.28968935844539345 1101 62-63 0.6264701155806556 1101 64-65 0.4786813794255913 1101 66-67 0.6428894971155579 1101 68-69 -0.1979155895960325 1101 70-71 -0.10543869064562372 1101 72-73 0.08638682808635068 1101 74-75 -0.3191158545260464 1101 76-77 -0.27670262628845776 1101 78-79 -0.5584915850547283 1101 80-81 -0.6114325892836483 1101 82-83 -0.176142550443533 1101 84-85 0.3999049841632605 1101 86-87 -0.0768189143404534 1101 88-89 0.0652216083322621 1101 90-91 0.039335853114597086 1101 92-93 0.2265477837773915 1101 94-95 -0.7474684880737215 1101 96-97 -0.4645828657749824 1101 98-99 -0.5329709318976299 1101 100-101 0.2987281197904217 1102 1 0.13600117250204136 1102 2 0.21446441887986367 1102 3 0.1230619018953405 1102 4 0.19007649257422088 1102 5 0.26059916371362135 1102 6 0.27409078309742085 1102 7 0.404482744849183 1102 8 0.08707002739177483 1102 9 0.2853199239918425 1102 10-11 0.11560654365148082 1102 12-13 0.19890856559089087 1102 14-15 0.4453849714189104 1102 16-17 0.6531640506697372 1102 18-19 0.5286807005308987 1102 20-21 0.3609534847746474 1102 22-23 0.47052398801876194 1102 24-25 -0.05592730215064279 1102 26-27 0.25669793680585684 1102 28-29 0.3700502597495188 1102 30-31 0.6909724672696029 1102 32-33 0.37987426927365675 1102 34-35 0.1916708258368658 1102 36-37 0.18933153059337116 1102 38-39 0.1078080878556662 1102 40-41 0.1162943310715363 1102 42-43 0.23897990748616138 1102 44-45 0.06260455939563059 1102 46-47 -0.006581104883665034 1102 48-49 -0.004843853940705856 1102 50-51 0.29846880347297855 1102 52-53 0.7281942539603108 1102 54-55 0.5264889364271212 1102 56-57 0.38902581116985857 1102 58-59 0.2144940832535127 1102 60-61 0.7551989857197299 1102 62-63 0.8078205836958254 1102 64-65 0.27573578677411703 1102 66-67 0.1404164358189668 1102 68-69 0.09911673684805677 1102 70-71 0.4119389811438161 1102 72-73 0.2635860861679653 1102 74-75 0.550297676652189 1102 76-77 0.36128094551864365 1102 78-79 0.14222383624892743 1102 80-81 0.5262197689569454 1102 82-83 0.7061220671152029 1102 84-85 0.8905868424921763 1102 86-87 0.32255840415450976 1102 88-89 -0.024625591809051883 1102 90-91 0.39532419439705535 1102 92-93 0.9843644239469747 1102 94-95 0.7588245696899207 1102 96-97 0.818958028060031 1102 98-99 1.2553615181995283 1102 100-101 2.0681372347877733 1103 1 0.24329197614743947 1103 2 0.2715849196623381 1103 3 0.40700740497920407 1103 4 0.18464521208080242 1103 5 0.17517156544426626 1103 6 0.16656984044806222 1103 7 0.3664177535944688 1103 8 0.25106708161252556 1103 9 0.029221240386945624 1103 10-11 0.3757326598256441 1103 12-13 0.08351686900615363 1103 14-15 0.10231675821813013 1103 16-17 0.250812030049282 1103 18-19 0.2834906057136308 1103 20-21 -0.2412742607836762 1103 22-23 0.06699825847811525 1103 24-25 -0.045248852366981396 1103 26-27 0.2734290423936372 1103 28-29 -0.03724974945603776 1103 30-31 0.11155149700356048 1103 32-33 0.08640101142592016 1103 34-35 -0.05200035155428395 1103 36-37 0.010168316011757383 1103 38-39 0.14651746832146273 1103 40-41 -0.36469618720149555 1103 42-43 -0.24891478090562913 1103 44-45 0.21698180325090988 1103 46-47 0.22220007895137428 1103 48-49 -0.10760091920812442 1103 50-51 -0.218354357716386 1103 52-53 -0.09439158788816115 1103 54-55 0.0914801911449672 1103 56-57 0.6403376034924193 1103 58-59 0.2708781392233135 1103 60-61 0.5145656431808376 1103 62-63 0.0335409187782183 1103 64-65 -0.1652565726109536 1103 66-67 -0.40602561517983915 1103 68-69 0.005358106635409143 1103 70-71 -0.23072418740999368 1103 72-73 0.27981088594702896 1103 74-75 0.36874101642941426 1103 76-77 0.22305945974123276 1103 78-79 0.4249496026118109 1103 80-81 0.5401661925968249 1103 82-83 0.32538009896644127 1103 84-85 0.29645998987319544 1103 86-87 0.2863805527322505 1103 88-89 0.563080382599491 1103 90-91 0.6593396597381194 1103 92-93 0.8417933109947491 1103 94-95 0.8429864034375072 1103 96-97 1.4378248236045366 1103 98-99 1.0456358438922493 1103 100-101 0.6313794330755371 1104 1 0.11138707154250937 1104 2 0.3752209200618921 1104 3 0.24223865060901062 1104 4 -0.18275065246819366 1104 5 -0.0015340583695362398 1104 6 -0.3785305491234112 1104 7 -0.04997421523981416 1104 8 -0.17311631664938432 1104 9 -0.2997169276282321 1104 10-11 0.24375283763006905 1104 12-13 0.06923258172216862 1104 14-15 0.1777587720029672 1104 16-17 0.007704448389112883 1104 18-19 0.1368019632203712 1104 20-21 0.42659108733339934 1104 22-23 -0.3451483802193138 1104 24-25 -0.16766419548954303 1104 26-27 0.044905418380047024 1104 28-29 -0.37497824810752434 1104 30-31 0.6011379519031692 1104 32-33 0.06003001951701492 1104 34-35 0.07805658581461472 1104 36-37 -0.01717660457559589 1104 38-39 -0.009560845533293616 1104 40-41 0.4495581330462315 1104 42-43 0.9395778771704926 1104 44-45 1.3273104417485726 1104 46-47 1.3501842963122712 1104 48-49 1.2726557984025035 1104 50-51 0.9379487089103336 1104 52-53 1.390549976391135 1104 54-55 0.8274206566329312 1104 56-57 0.1996223902270131 1104 58-59 0.07334542520872844 1104 60-61 0.43215629346551765 1104 62-63 0.2247306101177422 1104 64-65 0.4011703577656327 1104 66-67 0.7899088569008725 1104 68-69 1.0799260818626557 1104 70-71 1.1504315412884374 1104 72-73 1.1008827069439313 1104 74-75 1.055860185343608 1104 76-77 0.2555237490230233 1104 78-79 0.023116618893901375 1104 80-81 0.32555226791397374 1104 82-83 0.3374668035913544 1104 84-85 0.567961338389825 1104 86-87 0.4568929890522959 1104 88-89 0.605258986135599 1104 90-91 0.4013734225989758 1104 92-93 0.589495838214809 1104 94-95 0.8812692922598018 1104 96-97 0.994037989122468 1104 98-99 1.452413388593076 1104 100-101 1.0057676060852252 1105 1 0.23318787326930135 1105 2 0.1878943705706817 1105 3 0.2843293074082709 1105 4 0.11789689609794607 1105 5 0.2645775666690042 1105 6 -0.017753490844036435 1105 7 0.32406217082247224 1105 8 0.21310014958557844 1105 9 0.17761887255677777 1105 10-11 0.6261919372292084 1105 12-13 0.3149926803962373 1105 14-15 0.3236884667300757 1105 16-17 0.7028940876120586 1105 18-19 0.7057196320455361 1105 20-21 0.33246527789768976 1105 22-23 0.3717033003233965 1105 24-25 0.6718362670451512 1105 26-27 0.5749701732366574 1105 28-29 0.4955120386639891 1105 30-31 0.6305859938396452 1105 32-33 0.27934855174027007 1105 34-35 0.2331598852287371 1105 36-37 0.3802948724518487 1105 38-39 0.44703798271215334 1105 40-41 0.2167490056950072 1105 42-43 0.13738854630400965 1105 44-45 0.21861478957465152 1105 46-47 0.7416428223683909 1105 48-49 1.0551874049396588 1105 50-51 0.5834929580623509 1105 52-53 0.5976884284416997 1105 54-55 0.7043093399508464 1105 56-57 0.6652406449294475 1105 58-59 0.8802371920912435 1105 60-61 0.9132694602863651 1105 62-63 0.24970748338324356 1105 64-65 0.22216943269625133 1105 66-67 0.22083700984567756 1105 68-69 0.2593895416221379 1105 70-71 -0.35773990490335805 1105 72-73 0.14274209639814117 1105 74-75 -0.2650618004719938 1105 76-77 -0.0645502565273901 1105 78-79 -0.2245707076555874 1105 80-81 0.0942078337480794 1105 82-83 -0.02014960097232077 1105 84-85 0.3299872403324713 1105 86-87 0.2948516692866505 1105 88-89 -0.11598677396307622 1105 90-91 -0.19205858480158255 1105 92-93 -0.08426302703341904 1105 94-95 0.11176574616051127 1105 96-97 0.0951018189097006 1105 98-99 -0.14333129225799013 1105 100-101 0.2665600821875991 1106 1 -0.24358656002686985 1106 2 -0.2043389999235572 1106 3 -0.41188028010566313 1106 4 -0.25185412582698774 1106 5 -0.17429274176652143 1106 6 -0.4358136915129336 1106 7 -0.26419498265207153 1106 8 -0.43480025918439225 1106 9 -0.37682185579699023 1106 10-11 -1.10058249606697 1106 12-13 -1.4750481966011648 1106 14-15 -0.5350071854438383 1106 16-17 -0.5180275028747516 1106 18-19 -0.6744364437627155 1106 20-21 -0.5384934734958478 1106 22-23 -0.8887017535599213 1106 24-25 -0.42571111311092835 1106 26-27 0.10637113942024001 1106 28-29 -0.41753143959688543 1106 30-31 -0.6551813788842509 1106 32-33 -0.4602463943764121 1106 34-35 -1.2575498880897342 1106 36-37 -0.5017192486927016 1106 38-39 -0.5515684832856138 1106 40-41 -1.4822476498233854 1106 42-43 -1.4417641850226346 1106 44-45 -1.1855100710719455 1106 46-47 -1.212463457824839 1106 48-49 -0.9374733159819328 1106 50-51 -1.0434115384073621 1106 52-53 -0.6805538606701447 1106 54-55 0.12301985648744562 1106 56-57 -0.14616775343547062 1106 58-59 -0.20606901378017994 1106 60-61 0.0744548932109339 1106 62-63 -0.5054021412916043 1106 64-65 -0.9117956360514263 1106 66-67 -1.0413331871071279 1106 68-69 -1.183940075620523 1106 70-71 -0.8497503099572405 1106 72-73 -0.6964440797445555 1106 74-75 -0.7714720568822493 1106 76-77 -1.3330378076236364 1106 78-79 -1.4764287529918967 1106 80-81 -1.3659370937881512 1106 82-83 -1.3831187574197408 1106 84-85 -1.2416404073821319 1106 86-87 -0.9738216863432285 1106 88-89 -1.6747010065903503 1106 90-91 -1.1810961173173773 1106 92-93 -0.4175963603667512 1106 94-95 -0.6232769888822247 1106 96-97 -0.8215648477569637 1106 98-99 -0.8911945401212336 1106 100-101 0.052048748111051424 1107 1 0.23558431284479298 1107 2 0.09197972946603983 1107 3 -0.0062675201621971155 1107 4 0.05045423947350258 1107 5 0.31901097988365734 1107 6 0.2461972109704078 1107 7 0.2666617371810247 1107 8 0.06880025229013143 1107 9 -0.2755364395509474 1107 10-11 -0.05841949721106232 1107 12-13 0.2708297225051055 1107 14-15 0.05160698778450268 1107 16-17 -0.03007407397644357 1107 18-19 0.24217974844403045 1107 20-21 0.16845181218959482 1107 22-23 0.44739085024921366 1107 24-25 0.4161019294895141 1107 26-27 0.3141576661043999 1107 28-29 0.6533347021353961 1107 30-31 0.7452156868671551 1107 32-33 0.5054982721556485 1107 34-35 0.4170580936048651 1107 36-37 0.39336117394677217 1107 38-39 0.700803816330783 1107 40-41 0.8372556814909657 1107 42-43 0.9885810602832379 1107 44-45 0.8535571609086716 1107 46-47 0.5150081082294022 1107 48-49 -0.5702547952201016 1107 50-51 0.022440806972539917 1107 52-53 0.3201864314087288 1107 54-55 -0.38478685998067874 1107 56-57 -0.2699984284472521 1107 58-59 -0.05417453905106129 1107 60-61 0.07129182705573811 1107 62-63 -0.1643630404099845 1107 64-65 -0.1968080864182049 1107 66-67 -0.27688066674909706 1107 68-69 -0.20951380198165026 1107 70-71 -0.15084728821957682 1107 72-73 0.32758176317892307 1107 74-75 0.5597413491343062 1107 76-77 0.09441356864663675 1107 78-79 -0.39297764591874085 1107 80-81 -0.5260420806647943 1107 82-83 -0.1780578515714346 1107 84-85 0.22343126635096056 1107 86-87 0.814993173337772 1107 88-89 0.7891256304646319 1107 90-91 0.22155547431059475 1107 92-93 0.5535322485571328 1107 94-95 0.5907683708324285 1107 96-97 -0.08271970340000934 1107 98-99 -0.7706278801844988 1107 100-101 -0.48412461483396285 1108 1 0.3957296792893672 1108 2 0.35121655563199994 1108 3 0.34709407886869315 1108 4 0.3643851904123281 1108 5 0.3368183693445914 1108 6 0.1564940007947584 1108 7 0.10076228230519035 1108 8 0.08400315961902294 1108 9 0.45565677668164284 1108 10-11 0.28488758940311243 1108 12-13 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88-89 -0.24010271599206234 2316 90-91 -0.7994209036421616 2316 92-93 -0.6002630270334173 2316 94-95 -0.24690092050615675 2316 96-97 -0.6322315144236299 2316 98-99 -1.1499979589246543 2316 100-101 -1.2293529612906617 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 5.0 5 3.0 6 13.0 7 47.0 8 75.0 9 156.0 10 256.0 11 344.0 12 503.0 13 729.0 14 934.0 15 1327.0 16 1808.0 17 2365.0 18 2223.0 19 1440.0 20 1385.0 21 1373.0 22 1307.0 23 1346.0 24 1356.0 25 1597.0 26 1697.0 27 2094.0 28 2514.0 29 3039.0 30 3557.0 31 4413.0 32 5485.0 33 7193.0 34 10204.0 35 15330.0 36 21817.0 37 26515.0 38 14923.0 39 619.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.01964987495534 18.997499106823863 15.356198642372277 20.62665237584852 2 17.626007200411454 22.495571175495744 34.74841419509686 25.130007428995942 3 21.757671866963825 23.66492370992628 30.44031087490714 24.137093548202753 4 14.471541230927482 17.96316932396137 34.36196354077376 33.20332590433739 5 14.499121265377857 36.96186434623573 32.07452812665209 16.464486261734322 6 31.47036973541345 34.8862792159552 15.612320704040231 18.03103034459112 7 27.527287273558485 34.64555117435282 19.192525287159263 18.634636264929423 8 26.775101434367677 30.381021772672725 19.225384307674727 23.61849248528487 9 25.591462369278243 18.583919081090347 20.480456026058633 35.34416252357278 10-11 26.547231270358306 25.405023144179665 24.546759814846563 23.500985770615465 12-13 28.023917019087897 23.26337295690936 26.07262258543834 22.640087438564407 14-15 24.28281616092348 25.794688267901023 26.586876392936738 23.335619178238755 16-17 21.98268472484142 28.365906623235617 26.976184353391623 22.675224298531344 18-19 21.218355334590548 26.96796959826276 29.963140750900052 21.85053431624664 20-21 23.307760443453912 24.519972569861135 29.83813360763472 22.33413337905023 22-23 22.071975541459512 24.608906223212752 30.598177038687922 22.720941196639806 24-25 23.719569689696556 25.384307674724273 29.97957026115778 20.916552374421396 26-27 22.785587747871308 24.607477570146866 31.72002685867764 20.88690782330419 28-29 21.053703068746785 26.995471169781133 30.703540202297276 21.247285559174813 30-31 21.946254071661237 25.368592490999486 30.342090976627233 22.34306246071204 32-33 22.395208297617007 25.81076061489228 30.432453283044747 21.361577804445968 34-35 23.214897994171096 25.538959369106806 29.458826218641065 21.787316418081033 36-37 22.878807360420595 24.803203040173724 30.640679467398137 21.677310132007545 38-39 22.195911194925426 25.501100062860736 30.257443282473282 22.045545459740556 40-41 21.951968683924797 25.858620492599577 29.9974284244814 22.191982398994227 42-43 22.30041581526942 26.499292685366445 28.14147721589529 23.05881428346884 44-45 22.621292645294016 25.36287787873593 28.716640950911483 23.299188525058575 46-47 23.638136464940853 26.769029658837646 28.06088919366821 21.531944682553288 48-49 23.84993428195897 25.785402022972743 28.244471112635004 22.120192582433283 50-51 22.185553460197728 26.8329618835362 28.406980398879938 22.574504257386135 52-53 23.379907423281328 27.823018458197613 26.22114120806903 22.575932910452025 54-55 22.37449282816161 26.62902165838048 26.87296416938111 24.123521344076803 56-57 23.468841076632952 26.544373964226526 26.57366135207726 23.41312360706326 58-59 24.479613120749757 25.804331676095778 26.89153665923767 22.824518543916795 60-61 23.232756157494713 26.7618863935082 28.649137093548205 21.356220355448883 62-63 21.86839247957026 26.7993885364878 28.342691010914912 22.98952797302703 64-65 21.96804103091605 27.639079375964343 27.58514772272701 22.807731870392594 66-67 22.736656380364593 26.522944168238183 26.64580833190468 24.09459111949254 68-69 21.918038173609922 26.49508543345334 27.299059946282643 24.287816446654094 70-71 23.56491799531402 26.66938110749186 26.84010514886565 22.925595748328476 72-73 23.451697239842275 25.582176124349964 27.89802274415681 23.068103891650953 74-75 22.23948511343505 28.135893479627406 26.172209840562317 23.452411566375222 76-77 22.283773358477628 26.706168923938513 26.55366020915481 24.456397508429053 78-79 22.797374135664896 26.667595291159497 26.78117320989771 23.753857363277902 80-81 23.33704783130465 27.03904508829076 26.325790045145435 23.298117035259157 82-83 22.79610832124922 26.365266338550335 27.042809894921817 23.795815445278627 84-85 22.531367975912822 26.762339142019336 26.52161024062175 24.184682641446088 86-87 22.747371278358763 26.211497799874277 26.432581861820676 24.608549059946284 88-89 22.16983827647294 26.407937596434085 25.803260186296363 25.61896394079662 90-91 22.846804497849664 25.421137607338295 26.373430110445927 25.358627784366117 92-93 23.176272251907307 25.722204760408037 26.781938451867305 24.319584535817356 94-95 22.88559346248357 25.523244185382023 26.818675352877307 24.7724869992571 96-97 21.73552774444254 26.24149951425796 27.38942225270015 24.633550488599347 98-99 22.589942960822622 26.78948364722144 25.446902133343812 25.173671258612128 100-101 22.039728664863958 25.68574990444688 26.22299061614794 26.05153081454122 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 696.0 1 452.5 2 213.5 3 229.5 4 194.5 5 181.5 6 262.5 7 402.5 8 526.0 9 574.5 10 502.5 11 393.0 12 382.0 13 397.0 14 433.0 15 449.5 16 455.0 17 468.5 18 452.5 19 458.5 20 485.5 21 515.5 22 570.0 23 611.5 24 631.5 25 639.5 26 650.5 27 764.5 28 845.5 29 912.0 30 1047.5 31 1145.5 32 1183.0 33 1268.0 34 1445.0 35 1489.5 36 1486.5 37 1608.5 38 1869.5 39 2007.0 40 2051.5 41 2589.5 42 3312.5 43 3711.5 44 3790.0 45 3886.5 46 4101.5 47 5690.5 48 8934.0 49 8882.5 50 6269.0 51 5129.0 52 4827.5 53 4888.0 54 4996.5 55 4837.5 56 4257.5 57 3814.0 58 3630.0 59 3390.0 60 3125.0 61 2792.5 62 2438.0 63 2033.5 64 1616.0 65 1291.0 66 1005.0 67 898.0 68 703.5 69 422.0 70 355.5 71 318.0 72 300.5 73 247.0 74 174.0 75 129.5 76 90.5 77 59.0 78 26.5 79 15.0 80 6.5 81 2.5 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030001714383679066 2 0.0 3 0.0 4 0.0 5 0.012857877593005313 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005714612263557918 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01857248985656323 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.143265329447397E-4 84-85 3.5716326647236985E-4 86-87 0.0 88-89 0.0 90-91 0.010000571461226355 92-93 0.002857306131778959 94-95 0.0 96-97 0.0 98-99 3.5716326647236985E-4 100-101 0.012500714326532946 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 139992.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.23527058689068 #Duplication Level Percentage of deduplicated Percentage of total 1 72.19328214321956 30.491028058746217 2 13.630213442478775 11.513515058003314 3 5.3005445996685046 6.716098062746442 4 2.6807157595643205 4.52883021886965 5 1.4900382234549945 3.1466083776215785 6 0.9623515881338159 2.4387107834733412 7 0.597030071373 1.765100862906452 8 0.44312146940432295 1.4972284130521742 9 0.3162737205290397 1.2022115549459969 >10 2.0921422047830056 16.565232298988512 >50 0.1911172749720935 5.344591119492542 >100 0.08456516591685552 7.11040630893194 >500 0.011839123228359774 3.4087662152122977 >1k 0.006765213273348442 4.271672667009543 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2033 1.4522258414766558 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1358 0.9700554317389565 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1345 0.9607691868106749 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1244 0.8886222069832562 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 829 0.5921766958111893 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 820 0.5857477570146865 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 802 0.5728898794216812 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 634 0.452883021886965 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 568 0.4057374707126121 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 560 0.4000228584490543 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 559 0.3993085319161095 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 497 0.35502028687353565 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 435 0.31073204183096176 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 417 0.29787416423795643 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 376 0.2685867763872221 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 372 0.2657294702554432 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 363 0.2593005314589405 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 327 0.2335847762729299 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 319 0.22787016400937196 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 297 0.21215498028458768 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 295 0.2107263272186982 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 295 0.2107263272186982 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 292 0.208583347619864 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 258 0.18429624549974286 No Hit GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG 250 0.17858163323618492 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 242 0.17286702097262702 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 228 0.16286644951140067 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 227 0.1621521229784559 No Hit GATAATACGTCTCAATGAAGTCACATAAGTGGGGATCATTCTTGTCAGTA 217 0.1550088576490085 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 189 0.13500771472655582 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 180 0.12857877593005315 No Hit AGATGATGTGGCTCTGAAGAACTTTGCCAAATACTTTCTCCACCAATCTC 178 0.12715012286416366 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 172 0.12286416366649522 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 170 0.12143551060060576 No Hit CCCTGGGACACGGTGATGAGAGCTAAGCTGACTTCCCCAAAGCCACGTGA 164 0.11714955140293731 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 163 0.11643522486999257 No Hit TCCTACGTCTATCTGTCTATGTCTTGTTATTTTGACCGAGATGATGTGGC 157 0.11214926567232412 No Hit GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC 154 0.11000628607348992 No Hit GGTGAAATCCATTAAAGAACTGGGTGACCACGTGACCAACTTACGCAAGA 143 0.10214869421109778 No Hit CTCCCAGTCATCACGGTCTGGTTTCTTTATATCCTGCAGGAAGATTCGGC 142 0.10143436767815303 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 142 0.10143436767815303 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 141 0.10072004114520829 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.143265329447397E-4 2 0.0 0.0 0.0 0.0 7.143265329447397E-4 3 0.0 0.0 0.0 0.0 7.143265329447397E-4 4 0.0 0.0 0.0 0.0 7.143265329447397E-4 5 0.0 0.0 0.0 0.0 7.143265329447397E-4 6 0.0 0.0 0.0 0.0 0.002142979598834219 7 0.0 0.0 0.0 0.0 0.002142979598834219 8 0.0 0.0 0.0 0.0 0.002142979598834219 9 0.0 0.0 0.0 0.0 0.002142979598834219 10-11 0.0 0.0 0.0 0.0 0.002142979598834219 12-13 0.0 0.0 0.0 0.0 0.002500142865306589 14-15 0.0 0.0 0.0 0.0 0.0035716326647236983 16-17 0.0 0.0 0.0 3.5716326647236985E-4 0.0035716326647236983 18-19 0.0 0.0 0.0 7.143265329447397E-4 0.0035716326647236983 20-21 0.0 0.0 0.0 0.0010714897994171095 0.0035716326647236983 22-23 0.0 0.0 0.0 0.002142979598834219 0.005357448997085548 24-25 0.0 0.0 0.0 0.004643122464140808 0.007500428595919767 26-27 0.0 0.0 0.0 0.009643408194753986 0.007857591862392137 28-29 0.0 0.0 0.0 0.018215326590090862 0.007857591862392137 30-31 0.0 0.0 0.0 0.037502142979598835 0.007857591862392137 32-33 0.0 0.0 0.0 0.07214697982741872 0.007857591862392137 34-35 0.0 0.0 0.0 0.10714897994171096 0.008571918395336876 36-37 0.0 0.0 0.0 0.14893708211897821 0.008571918395336876 38-39 0.0 0.0 0.0 0.2096548374192811 0.008571918395336876 40-41 0.0 0.0 0.0 0.2632293273901366 0.008571918395336876 42-43 0.0 0.0 0.0 0.30394593976798673 0.008929081661809245 44-45 0.0 0.0 0.0 0.33251900108577637 0.009286244928281616 46-47 0.0 0.0 0.0 0.3732356134636265 0.009286244928281616 48-49 0.0 0.0 0.0 0.4100234299102806 0.010000571461226355 50-51 0.0 0.0 0.0 0.4439539402251557 0.010000571461226355 52-53 0.0 0.0 0.0 0.4982427567289559 0.010714897994171095 54-55 0.0 0.0 0.0 0.612177838733642 0.011429224527115835 56-57 0.0 0.0 0.0 0.7507571861249214 0.011429224527115835 58-59 0.0 0.0 0.0 0.8746928395908338 0.011429224527115835 60-61 0.0 0.0 0.0 1.0125578604491685 0.011429224527115835 62-63 0.0 0.0 0.0 1.0997056974684267 0.011429224527115835 64-65 0.0 0.0 0.0 1.2182839019372536 0.011429224527115835 66-67 0.0 0.0 0.0 1.4122235556317504 0.011429224527115835 68-69 0.0 0.0 0.0 1.6747385564889423 0.011429224527115835 70-71 0.0 0.0 0.0 1.9772558431910394 0.012143551060060575 72-73 0.0 0.0 0.0 2.2672724155666035 0.012857877593005315 74-75 0.0 0.0 0.0 2.580504600262872 0.012857877593005315 76-77 0.0 0.0 0.0 2.8619492542430995 0.012857877593005315 78-79 0.0 0.0 0.0 3.1573232756157497 0.012857877593005315 80-81 0.0 0.0 0.0 3.4598405623178468 0.013215040859477684 82-83 0.0 0.0 0.0 3.802002971598377 0.013572204125950055 84-85 0.0 0.0 0.0 4.347391279501686 0.013572204125950055 86-87 0.0 0.0 0.0 4.983856220355449 0.013572204125950055 88-89 0.0 0.0 0.0 5.6899679981713245 0.014643693925367164 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATGTA 25 0.005007537 57.013077 2 GTACACG 25 0.005007537 57.013077 4 TATGTCA 25 0.005007537 57.013077 5 TATGTAG 25 0.005007537 57.013077 3 ACACGAG 25 0.005014628 56.99271 6 GTTGCAC 60 0.003934782 31.673935 8 CGAGACC 30 0.003948363 31.662619 22-23 CTTAATA 65 0.0058202436 29.237478 2 CCTACAC 70 0.008357483 27.149086 3 CCGATGG 35 0.008386149 27.139387 16-17 TTGGTAT 35 0.008386149 27.139387 16-17 GATTCGT 55 0.002874122 21.588148 66-67 GAGTACT 1080 0.0 21.335913 12-13 GTACTTT 1100 0.0 20.508741 14-15 CCGAGGT 60 0.0047483547 19.79621 86-87 CGTCAGA 60 0.004758184 19.789137 70-71 CTTCTTA 125 0.0059973276 19.00436 9 ACTTTTT 1255 0.0 18.543446 16-17 CAAATTA 155 9.5523486E-4 18.391315 4 CCCCCAT 65 0.0075392174 18.273424 86-87 >>END_MODULE