##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937808_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 122948 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.750699482708136 33.0 31.0 34.0 30.0 34.0 2 31.83820802290399 33.0 31.0 34.0 30.0 34.0 3 31.84896866968149 33.0 31.0 34.0 30.0 34.0 4 35.4504424634805 37.0 35.0 37.0 33.0 37.0 5 35.45637993297979 37.0 35.0 37.0 33.0 37.0 6 35.5458079838631 37.0 35.0 37.0 33.0 37.0 7 35.48469271561961 37.0 35.0 37.0 33.0 37.0 8 35.49907277873572 37.0 35.0 37.0 33.0 37.0 9 37.053746299248466 39.0 37.0 39.0 34.0 39.0 10-11 36.988043725802775 39.0 37.0 39.0 33.0 39.0 12-13 36.89095389920942 39.0 37.0 39.0 33.0 39.0 14-15 37.970869798614046 40.0 38.0 41.0 33.0 41.0 16-17 37.85821648176465 40.0 38.0 41.0 33.0 41.0 18-19 37.817622084133134 40.0 38.0 41.0 33.0 41.0 20-21 37.8229007385236 40.0 38.0 41.0 33.0 41.0 22-23 37.79873198425351 40.0 38.0 41.0 33.0 41.0 24-25 37.6819753066337 40.0 37.5 41.0 33.0 41.0 26-27 37.51937404431142 40.0 37.0 41.0 33.0 41.0 28-29 37.365703386797676 40.0 37.0 41.0 32.5 41.0 30-31 37.07141230438884 39.0 36.0 41.0 32.0 41.0 32-33 36.81377086247845 39.0 35.0 41.0 31.0 41.0 34-35 36.69561115268243 39.0 35.0 41.0 31.0 41.0 36-37 36.47789715977486 39.0 35.0 41.0 31.0 41.0 38-39 36.25660441812799 39.0 35.0 41.0 30.0 41.0 40-41 35.96777092754661 39.0 35.0 40.5 30.0 41.0 42-43 35.59377135048963 38.0 35.0 40.0 29.0 41.0 44-45 35.198669356150575 38.0 35.0 40.0 26.5 41.0 46-47 34.633170120701436 38.0 34.5 40.0 24.0 41.0 48-49 34.0698466018154 38.0 34.0 40.0 22.0 41.0 50-51 33.0344576568956 37.0 32.5 39.5 19.0 40.5 52-53 33.08180694277256 37.0 33.0 39.5 18.0 40.0 54-55 33.250235872076 37.0 33.0 40.0 14.0 41.0 56-57 32.90072632332368 37.0 33.0 40.0 9.0 41.0 58-59 32.548052835345025 36.0 33.0 40.0 6.5 41.0 60-61 32.1123523766145 36.0 32.0 39.0 2.0 41.0 62-63 31.6794986498357 35.0 31.0 39.0 2.0 40.5 64-65 31.226189120603834 35.0 31.0 39.0 2.0 40.0 66-67 30.611404821550572 35.0 30.5 38.0 2.0 40.0 68-69 30.112872921885675 35.0 29.5 37.0 2.0 39.5 70-71 29.571253700751537 34.5 29.0 36.5 2.0 39.0 72-73 29.10613430067996 34.0 29.0 36.0 2.0 39.0 74-75 28.57855760158766 34.0 28.0 35.5 2.0 38.0 76-77 28.098627061847285 34.0 27.0 35.0 2.0 37.0 78-79 27.57782965156001 34.0 26.0 35.0 2.0 37.0 80-81 27.06077772716921 33.0 25.5 35.0 2.0 36.0 82-83 26.52714968929954 33.0 24.5 35.0 2.0 36.0 84-85 26.035039203565738 32.5 23.5 35.0 2.0 35.0 86-87 25.545535510947715 32.0 20.5 35.0 2.0 35.0 88-89 25.002305852880895 32.0 18.5 34.5 2.0 35.0 90-91 24.44947050785698 31.5 14.5 34.0 2.0 35.0 92-93 23.803965904284738 31.0 4.5 34.0 2.0 35.0 94-95 23.079159482057456 31.0 2.0 34.0 2.0 35.0 96-97 22.255681263623647 30.0 2.0 34.0 2.0 35.0 98-99 21.156041578553534 29.5 2.0 34.0 2.0 35.0 100-101 18.216144223574194 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8684920706402757 1101 2 0.7053715696257505 1101 3 0.6514290966547271 1101 4 0.39879036537957546 1101 5 0.35577823452758395 1101 6 0.3206557401890251 1101 7 0.8099037509179396 1101 8 0.9019067034212895 1101 9 0.9125050298324524 1101 10-11 0.9790905374007082 1101 12-13 0.9768608840377695 1101 14-15 0.8799024559396145 1101 16-17 1.0125075440022044 1101 18-19 1.3293414731175375 1101 20-21 0.9597444869935146 1101 22-23 1.051846926063412 1101 24-25 0.9168390981826988 1101 26-27 1.452734487104813 1101 28-29 1.2401943221177945 1101 30-31 0.5752400396877277 1101 32-33 0.3664999449885826 1101 34-35 0.6934388673708227 1101 36-37 0.7664966926599135 1101 38-39 0.9046967281805962 1101 40-41 1.0410268014492559 1101 42-43 1.321586400097182 1101 44-45 1.7418007151655814 1101 46-47 1.8270525057437936 1101 48-49 1.8610025210931482 1101 50-51 1.9453737387749541 1101 52-53 2.0538025159838114 1101 54-55 2.5512405595166996 1101 56-57 2.6136449716492365 1101 58-59 2.9635736880010555 1101 60-61 2.132764451160199 1101 62-63 2.3349312149782158 1101 64-65 2.4235700981640242 1101 66-67 2.6182267685269345 1101 68-69 2.486962227966515 1101 70-71 2.754670580500438 1101 72-73 3.1742773853798774 1101 74-75 3.2829236767090357 1101 76-77 2.2536487330172434 1101 78-79 2.227458590907208 1101 80-81 2.4156833733457788 1101 82-83 2.424746050508272 1101 84-85 2.7182724468599133 1101 86-87 2.535977423947802 1101 88-89 3.0952018155650087 1101 90-91 3.415649994523303 1101 92-93 3.095438613296796 1101 94-95 2.8798690861621985 1101 96-97 3.74894998884443 1101 98-99 4.153275450452281 1101 100-101 4.732665989965017 1102 1 0.38606658245938874 1102 2 0.644939914949493 1102 3 0.548448623890085 1102 4 0.6288520303333271 1102 5 0.45043393853580227 1102 6 0.4647461821211891 1102 7 0.615299434371174 1102 8 0.5494914927326988 1102 9 0.8347558006443734 1102 10-11 0.8158068786134436 1102 12-13 1.160699527408788 1102 14-15 1.1709610376456823 1102 16-17 0.846114943796664 1102 18-19 1.1502304762007896 1102 20-21 1.2871083102206455 1102 22-23 0.7894882415824256 1102 24-25 1.106253281122065 1102 26-27 0.692816707043157 1102 28-29 1.025497508140404 1102 30-31 1.4926860828531971 1102 32-33 1.0911659264891043 1102 34-35 0.8342148180388591 1102 36-37 0.9050372887544711 1102 38-39 1.0438796675433863 1102 40-41 1.317003163216981 1102 42-43 1.6841506344240074 1102 44-45 2.0401820101296124 1102 46-47 2.179262166586554 1102 48-49 2.070509201463146 1102 50-51 1.7047262567605657 1102 52-53 2.26939861053674 1102 54-55 1.7905005800716864 1102 56-57 1.8655206139925014 1102 58-59 1.82999198193734 1102 60-61 1.822589733791233 1102 62-63 2.183749303364646 1102 64-65 2.1141661927169544 1102 66-67 2.2509348877458457 1102 68-69 2.260240748007625 1102 70-71 2.2943417007471005 1102 72-73 2.975176665955413 1102 74-75 2.8082320014777906 1102 76-77 2.4359457525444768 1102 78-79 3.0956240585331045 1102 80-81 3.4683812150723945 1102 82-83 3.1277265232729157 1102 84-85 3.5048603194190058 1102 86-87 3.24892706423557 1102 88-89 3.065628331495123 1102 90-91 2.912900765335227 1102 92-93 1.995541388219717 1102 94-95 2.579689230047091 1102 96-97 2.945095929234977 1102 98-99 2.612422418592054 1102 100-101 2.2801993918149677 1103 1 -0.13139373500092688 1103 2 0.14176531177488982 1103 3 -0.003932328490868997 1103 4 -0.08305273157143489 1103 5 -0.01147082336895977 1103 6 0.23141284878786195 1103 7 -0.18866881959707626 1103 8 -0.39654025329905096 1103 9 0.2339621498507256 1103 10-11 0.357473545280115 1103 12-13 0.0476042893135471 1103 14-15 0.6638181805028225 1103 16-17 0.4238927215744326 1103 18-19 -0.35730920633889696 1103 20-21 0.3025845006968382 1103 22-23 0.5597263368205248 1103 24-25 0.3594278842966716 1103 26-27 0.17535638958283783 1103 28-29 0.3651800478229461 1103 30-31 -0.27199645682934204 1103 32-33 -0.08105629573312001 1103 34-35 0.4219132307372746 1103 36-37 0.3883706220878054 1103 38-39 0.6998320484957716 1103 40-41 0.8443841155979399 1103 42-43 0.8901030153763898 1103 44-45 0.6024835974311955 1103 46-47 1.1205320078563972 1103 48-49 1.3863822173361555 1103 50-51 0.7586945107288159 1103 52-53 1.1578906740288062 1103 54-55 1.0317704213415269 1103 56-57 0.7730602965321793 1103 58-59 1.4486427755881337 1103 60-61 1.1408437020452027 1103 62-63 1.1218445414598825 1103 64-65 0.8609915895423548 1103 66-67 0.9314904433013993 1103 68-69 0.8173836051504821 1103 70-71 0.9792623356677339 1103 72-73 1.4985893643681152 1103 74-75 1.8102161284619172 1103 76-77 2.112533054131781 1103 78-79 1.3658621537712001 1103 80-81 0.6148097865009667 1103 82-83 0.3301074756538682 1103 84-85 0.729463494022184 1103 86-87 0.9401969055054131 1103 88-89 1.1295172203840096 1103 90-91 0.883932511366968 1103 92-93 1.3122080548863835 1103 94-95 1.5729431983010578 1103 96-97 2.082397516536563 1103 98-99 2.0568668630365003 1103 100-101 3.0262311378467146 1104 1 0.4245119606106478 1104 2 0.30376344436126246 1104 3 0.5135746743102523 1104 4 0.5191881647871099 1104 5 0.6369885603845447 1104 6 0.36831761069262114 1104 7 0.6755935520182348 1104 8 0.3505419129007663 1104 9 0.6158482376191614 1104 10-11 0.6487900718907582 1104 12-13 0.3723591912743345 1104 14-15 0.5325156594944218 1104 16-17 0.4123964564017015 1104 18-19 0.48541955183104335 1104 20-21 0.5921503270273689 1104 22-23 0.582660510489994 1104 24-25 0.43482470969349407 1104 26-27 0.7039511608246656 1104 28-29 0.9032286005773784 1104 30-31 1.2897449063826087 1104 32-33 1.235178531531119 1104 34-35 1.659424902072466 1104 36-37 1.0665919106032078 1104 38-39 1.3608964742660774 1104 40-41 1.2733056842253845 1104 42-43 1.2754321470290506 1104 44-45 1.2615055395413748 1104 46-47 1.6658167884352935 1104 48-49 1.7434083611270026 1104 50-51 2.103990962642918 1104 52-53 2.3696086945703456 1104 54-55 2.235143437214539 1104 56-57 1.2107937232362005 1104 58-59 1.111399544962552 1104 60-61 1.230362843034932 1104 62-63 1.2613753537848176 1104 64-65 1.8496703943976378 1104 66-67 2.215745812115589 1104 68-69 2.3709550337219127 1104 70-71 2.4520097679739905 1104 72-73 1.9950296071318867 1104 74-75 2.7121185560996395 1104 76-77 2.6943491138890145 1104 78-79 2.630918176180163 1104 80-81 2.3338223915429843 1104 82-83 1.955982825793928 1104 84-85 2.3001334286627078 1104 86-87 2.6945152995296873 1104 88-89 2.646090516369071 1104 90-91 3.141052025839425 1104 92-93 3.0510035730936664 1104 94-95 3.5348362888706184 1104 96-97 2.9851169376383346 1104 98-99 4.466939225314743 1104 100-101 4.82116577836959 1105 1 0.0235802138661505 1105 2 -0.02223565756958834 1105 3 -0.04827983848723605 1105 4 -0.6673857014005904 1105 5 -0.39242320432133937 1105 6 -0.025766358773026354 1105 7 -0.2972959854761541 1105 8 0.3424031612168861 1105 9 0.5237961496083869 1105 10-11 0.4018210552764714 1105 12-13 0.011980879644340803 1105 14-15 0.4830657268496026 1105 16-17 0.03815420224812982 1105 18-19 0.3415881643142029 1105 20-21 0.6189240136012444 1105 22-23 0.6231578162825357 1105 24-25 0.2707025722889185 1105 26-27 0.37340558381523437 1105 28-29 0.11393807520692434 1105 30-31 -0.40597803928420717 1105 32-33 0.04492819475519383 1105 34-35 -0.43762269559254463 1105 36-37 0.31103510772938137 1105 38-39 0.7261861015673858 1105 40-41 0.3990914947327937 1105 42-43 0.569128824173184 1105 44-45 1.1716705597479233 1105 46-47 1.2356416649507835 1105 48-49 0.9323957881588925 1105 50-51 0.6898407605773542 1105 52-53 0.22580537171449322 1105 54-55 0.5896281700389707 1105 56-57 0.6892119989434278 1105 58-59 0.45679132762981567 1105 60-61 1.154868905001706 1105 62-63 1.2763338180865667 1105 64-65 0.7635118851924219 1105 66-67 0.9950733828385374 1105 68-69 0.4100771711264919 1105 70-71 0.5376350486205972 1105 72-73 0.620787352978315 1105 74-75 0.9520433428082136 1105 76-77 1.0920253599599619 1105 78-79 0.587417951662875 1105 80-81 0.7590300700342318 1105 82-83 0.8763633825967965 1105 84-85 1.1726356738355825 1105 86-87 1.0054156138538914 1105 88-89 0.821326259521296 1105 90-91 0.8895365341465649 1105 92-93 0.6021935147191719 1105 94-95 -0.04607412873153649 1105 96-97 0.6826640862687832 1105 98-99 0.04072121902825998 1105 100-101 0.566731561934926 1106 1 -0.4523262746834682 1106 2 -0.3800600850505056 1106 3 0.14041290960436825 1106 4 -0.4550765410952451 1106 5 -0.31950653765467507 1106 6 0.03498427735928544 1106 7 -0.5492838989621589 1106 8 -0.061222792981588725 1106 9 -0.17030372316514786 1106 10-11 -0.5993716928151258 1106 12-13 -0.2857290440197815 1106 14-15 -0.8771044385447979 1106 16-17 -0.22516481810810518 1106 18-19 0.368385238105553 1106 20-21 0.014638072125407575 1106 22-23 0.15496443205861254 1106 24-25 0.48169970969349407 1106 26-27 -0.21179638819494784 1106 28-29 0.33889036528326244 1106 30-31 0.41872774951986713 1106 32-33 0.8249456883938606 1106 34-35 0.39686362756267357 1106 36-37 -0.7388615207693405 1106 38-39 -0.7034417610280386 1106 40-41 -1.04624088440206 1106 42-43 -0.4923374608140847 1106 44-45 -0.583776323203729 1106 46-47 -0.28026164293725486 1106 48-49 -0.6101455604416159 1106 50-51 -0.6983987432394372 1106 52-53 -0.6377442466061183 1106 54-55 -0.3638148961187966 1106 56-57 -0.8320984336265482 1106 58-59 -0.722240160919803 1106 60-61 -0.354195980494481 1106 62-63 -0.9763697442543986 1106 64-65 -0.585684997759234 1106 66-67 0.09840512584107941 1106 68-69 -0.884253299611423 1106 70-71 -0.2747059183005227 1106 72-73 -1.3144066673779164 1106 74-75 -0.33016085566506703 1106 76-77 0.5616898001635278 1106 78-79 1.494582391866441 1106 80-81 0.7881728817390616 1106 82-83 0.36805390422529527 1106 84-85 0.12703293846663044 1106 86-87 0.8176770642355713 1106 88-89 0.6684557124474999 1106 90-91 1.655757908192367 1106 92-93 1.6462854358387666 1106 94-95 1.4640642300470894 1106 96-97 1.8395899768540218 1106 98-99 1.7567676566872912 1106 100-101 1.861300582291161 1107 1 0.019645374801068982 1107 2 0.090300739691763 1107 3 0.007358785893028852 1107 4 0.14350593313155713 1107 5 -0.26871186411515424 1107 6 -0.13554836868882347 1107 7 -0.5274840707834656 1107 8 -0.2197279476207612 1107 9 -0.4156860255706505 1107 10-11 -0.5764979814749154 1107 12-13 -0.16717234298885586 1107 14-15 -0.5883909505722897 1107 16-17 -0.33915751570260255 1107 18-19 -0.49877541481541243 1107 20-21 0.06903025425599196 1107 22-23 -0.10104931364585212 1107 24-25 0.04822677154916022 1107 26-27 -0.018228948332399852 1107 28-29 -0.04931839760333645 1107 30-31 -0.7503551542602054 1107 32-33 -0.49985027380544267 1107 34-35 -1.0618455648771956 1107 36-37 -0.38421160667999743 1107 38-39 -0.006598977522884297 1107 40-41 -0.6418701799347062 1107 42-43 -0.6410702786835003 1107 44-45 -0.13945716512812112 1107 46-47 -0.2553475535901839 1107 48-49 -0.7358972786546829 1107 50-51 -1.2246551865384063 1107 52-53 -0.5720225971215811 1107 54-55 -0.6069957380431887 1107 56-57 -0.3608785023550638 1107 58-59 -0.68116412999197 1107 60-61 -0.3741702072986044 1107 62-63 0.12307183650161235 1107 64-65 -0.6043168706114663 1107 66-67 -0.21500767484620198 1107 68-69 -0.10456043019561179 1107 70-71 -0.5934344406372922 1107 72-73 -0.524565602085822 1107 74-75 -0.0965912680362031 1107 76-77 -0.23507781152032337 1107 78-79 0.5335106564712504 1107 80-81 0.5047214212579654 1107 82-83 0.8677854334349213 1107 84-85 0.9591313061642239 1107 86-87 0.606659022998457 1107 88-89 1.6198624993890824 1107 90-91 1.9173558463366973 1107 92-93 1.7180745251858447 1107 94-95 1.3082974774697682 1107 96-97 1.4516496847578004 1107 98-99 1.9493149075463982 1107 100-101 3.0832185376176184 1108 1 0.016997119811943406 1108 2 0.06145367641738275 1108 3 -0.2429013105791178 1108 4 -0.5668884677007497 1108 5 -0.1905883419360208 1108 6 0.10799650977519093 1108 7 -0.3704803821425813 1108 8 0.23350197766061598 1108 9 0.1744019343272072 1108 10-11 0.1377269310380882 1108 12-13 0.2968397633196673 1108 14-15 0.4675534360729472 1108 16-17 0.483598561096791 1108 18-19 0.0657285102767986 1108 20-21 0.02003753695721855 1108 22-23 0.03264027303720951 1108 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2316 92-93 -5.088089564161237 2316 94-95 -5.202602436619575 2316 96-97 -4.957285023145975 2316 98-99 -5.836982343312705 2316 100-101 -5.925157751042175 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 774.0 3 159.0 4 125.0 5 124.0 6 137.0 7 149.0 8 210.0 9 238.0 10 294.0 11 385.0 12 520.0 13 796.0 14 835.0 15 1156.0 16 1447.0 17 2054.0 18 1793.0 19 1277.0 20 1232.0 21 1366.0 22 1376.0 23 1492.0 24 1563.0 25 1735.0 26 1947.0 27 2133.0 28 2496.0 29 3093.0 30 3663.0 31 4346.0 32 5548.0 33 7322.0 34 10000.0 35 14083.0 36 18074.0 37 18946.0 38 9627.0 39 433.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.68249669641255 17.921757997944436 15.257679821198428 21.13806548444459 2 16.918574316832974 21.34204004434879 36.22334181177852 25.51604382703972 3 20.663171712065747 23.424620677847262 31.32984924950878 24.582358360578215 4 13.545683561744347 17.277166779445455 35.48292420450191 33.69422545430829 5 14.167733309418795 35.972085211847485 33.13250556420646 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22.747085326011682 52-53 22.152827006282237 26.939059864577473 27.494357394868285 23.413755734272 54-55 21.539407913765782 25.767198569281096 28.235921962268456 24.457471554684666 56-57 22.502688347236706 25.86067844108446 27.68671793534932 23.94991527632951 58-59 23.526488987358267 25.250472435813894 28.10260002606542 23.120438550762415 60-61 22.730253644588004 25.649997352352944 29.846314271632295 21.773434731426754 62-63 21.256542370119952 25.98171191169582 29.57212390281653 23.189621815367698 64-65 21.303956431594425 26.80988517264836 28.723131255117128 23.163027140640082 66-67 21.880829986395 25.78432410854671 27.876805513690538 24.45804039136775 68-69 21.144023266612898 25.732987918632027 28.821353797525074 24.30163501723 70-71 22.855839416058394 25.66801355578728 27.82113529718457 23.65501173096976 72-73 22.271377418211593 24.810066766878087 29.56114729856893 23.357408516341387 74-75 21.428193147051992 26.8015366377291 28.01756622981941 23.7527039853995 76-77 21.45545796737767 25.641610585148854 27.949371832684257 24.953559614789224 78-79 22.338046413863054 25.275472835628772 28.472790819880483 23.91368993062769 80-81 22.641947547234377 25.92452276782441 27.753199879418926 23.680329805522288 82-83 22.174745417515275 24.764969450101834 29.012219959266805 24.04806517311609 84-85 22.260147037859195 25.01313565362605 28.184835957069833 24.54188135144492 86-87 22.222403258655802 25.149898167006107 28.022810590631366 24.60488798370672 88-89 21.769328222320894 25.136974861803058 27.410859407779764 25.682837508096284 90-91 22.04950853995837 24.427580419808304 28.072898208910235 25.450012831323093 92-93 22.663543788187372 24.268431771894093 28.723421588594704 24.34460285132383 94-95 22.09119496855346 24.107928438752566 28.598021963697985 25.20285462899599 96-97 21.226403565230854 24.749268785491402 28.95813134975273 25.066196299525018 98-99 21.89121809338363 25.23769563552521 27.314426312316176 25.55665995877498 100-101 22.052288806252825 23.358898016870246 28.507541106463396 26.08127207041353 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 482.0 1 332.0 2 205.0 3 237.5 4 199.5 5 164.5 6 223.0 7 379.5 8 550.5 9 609.0 10 503.0 11 364.5 12 354.0 13 379.5 14 383.0 15 388.0 16 401.5 17 410.5 18 417.0 19 463.0 20 480.5 21 485.5 22 539.5 23 575.0 24 593.5 25 624.0 26 643.5 27 715.0 28 794.5 29 856.5 30 933.0 31 1044.5 32 1145.0 33 1191.5 34 1303.5 35 1351.5 36 1325.0 37 1411.0 38 1677.0 39 1791.0 40 1833.0 41 2334.0 42 2902.0 43 3161.5 44 3281.0 45 3415.0 46 3752.0 47 5129.0 48 7787.5 49 7825.0 50 5545.5 51 4456.0 52 4284.5 53 4440.0 54 4435.5 55 4173.5 56 3668.5 57 3273.5 58 3038.0 59 2773.5 60 2579.0 61 2443.0 62 2117.5 63 1663.0 64 1308.0 65 1047.0 66 795.0 67 662.5 68 525.5 69 351.5 70 292.5 71 233.0 72 209.5 73 174.0 74 118.5 75 101.0 76 59.5 77 26.5 78 20.5 79 11.0 80 3.0 81 4.5 82 4.0 83 2.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2879266031167648 2 0.23099196408237632 3 0.23993883593063733 4 0.23505872401340405 5 0.23505872401340405 6 0.1797507889514266 7 0.1732439730617822 8 0.17812408497901552 9 0.17812408497901552 10-11 0.1801574649445294 12-13 0.18097081693073497 14-15 0.18259752090314604 16-17 0.17446400104109056 18-19 0.16917721313075448 20-21 0.16917721313075448 22-23 0.17487067703419332 24-25 0.17243062107557666 26-27 0.17609070501350166 28-29 0.1752773530272961 30-31 0.17649738100660442 32-33 0.17649738100660442 34-35 0.17893743696522108 36-37 0.17243062107557666 38-39 0.16795718515144614 40-41 0.16267039724111007 42-43 0.1703972411100628 44-45 0.16999056511696004 46-47 0.17121059309626835 48-49 0.17405732504798777 50-51 0.16836386114454893 52-53 0.1797507889514266 54-55 0.17283729706867942 56-57 0.15941698929628786 58-59 0.14640335751699907 60-61 0.16063701727559618 62-63 0.15494355337215732 64-65 0.16145036926180173 66-67 0.16185704525490452 68-69 0.16023034128249342 70-71 0.15941698929628786 72-73 0.1687705371376517 74-75 0.17283729706867942 76-77 0.17080391710316556 78-79 0.16633048117903504 80-81 0.16999056511696004 82-83 0.16104369326869894 84-85 0.15494355337215732 86-87 0.16104369326869894 88-89 0.16795718515144614 90-91 0.16389042522041838 92-93 0.16104369326869894 94-95 0.16267039724111007 96-97 0.16836386114454893 98-99 0.16836386114454893 100-101 0.1525034974135407 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 122948.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.75592933597944 #Duplication Level Percentage of deduplicated Percentage of total 1 74.79913253697383 34.22503822754335 2 12.731086461888511 11.650453850408303 3 4.824374288964733 6.62231187168559 4 2.422852673492605 4.434395028792661 5 1.2514220705346986 2.862998991443537 6 0.7643629124004551 2.0984481244103197 7 0.5457195676905575 1.7478934183557275 8 0.4017349260523322 1.4705403910596349 9 0.26486063708759955 1.090705013501643 >10 1.75981228668942 15.424407066402058 >50 0.14753981797497154 4.501089891661516 >100 0.07465870307167236 7.89114097016625 >500 0.005332764505119454 2.008166053941504 >1k 0.007110352673492605 3.9724111006279075 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1262 1.0264502065914045 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1237 1.0061164069362658 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1213 0.9865959592673326 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1172 0.9532485278329049 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 964 0.7840713147021505 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 802 0.6523082929368514 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 703 0.5717864463025019 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 457 0.3717018576959365 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 435 0.35380811399941436 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 430 0.34974135406838663 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 415 0.3375410742753034 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 404 0.32859420242704235 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 393 0.31964733057878125 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 376 0.3058203468132869 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 374 0.3041936428408758 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 364 0.2960601229788203 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 343 0.2789797312685038 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 336 0.2732862673650649 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 280 0.2277385561375541 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 277 0.22529850017893746 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 258 0.20984481244103198 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 243 0.19764453264794873 No Hit GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG 232 0.18869766079968767 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 230 0.1870709568272766 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 227 0.1846309008686599 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 226 0.18381754888245436 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 218 0.17731073299280997 No Hit GTTCCAGTAGTGACTGATTCACACTCTTTTCCAAGTGCAGTGCACACTCC 203 0.16511045319972673 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 194 0.15779028532387676 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 187 0.1520968214204379 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 178 0.14477665354458796 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 174 0.14152324559976576 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 171 0.1390831896411491 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 155 0.1260695578618603 No Hit CCCTGGGACACGGTGATGAGAGCTAAGCTGACTTCCCCAAAGCCACGTGA 152 0.12362950190324365 No Hit GATAATACGTCTCAATGAAGTCACATAAGTGGGGATCATTCTTGTCAGTA 150 0.12200279793083255 No Hit CTCCATTGCATTCAGCCCGCTCTCCCAGTCATCACGGTCTGGTTTCTTTA 148 0.12037609395842144 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 146 0.11874938998601035 No Hit AGATGATGTGGCTCTGAAGAACTTTGCCAAATACTTTCTCCACCAATCTC 137 0.11142922211016039 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 134 0.10898916615154375 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 132 0.10736246217913263 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 129 0.10492240622051599 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 128 0.10410905423431044 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.133519862055503E-4 2 0.0 0.0 0.0 0.0 0.0016267039724111006 3 0.0 0.0 0.0 0.0 0.0016267039724111006 4 0.0 0.0 0.0 0.0 0.0016267039724111006 5 0.0 0.0 0.0 0.0 0.0016267039724111006 6 0.0 0.0 0.0 0.0 0.003253407944822201 7 0.0 0.0 0.0 0.0 0.003253407944822201 8 0.0 0.0 0.0 0.0 0.003253407944822201 9 0.0 0.0 0.0 0.0 0.004066759931027752 10-11 0.0 0.0 0.0 0.0 0.004066759931027752 12-13 0.0 0.0 0.0 0.0 0.004066759931027752 14-15 0.0 0.0 0.0 0.0 0.004473435924130527 16-17 0.0 0.0 0.0 0.0 0.004880111917233302 18-19 0.0 0.0 0.0 0.0 0.0056934639034388525 20-21 0.0 0.0 0.0 0.0 0.0061001398965416275 22-23 0.0 0.0 0.0 0.0 0.007726843868952728 24-25 0.0 0.0 0.0 0.0020333799655138755 0.010166899827569378 26-27 0.0 0.0 0.0 0.007726843868952728 0.011386927806877705 28-29 0.0 0.0 0.0 0.020333799655138755 0.011386927806877705 30-31 0.0 0.0 0.0 0.04676773920681914 0.011386927806877705 32-33 0.0 0.0 0.0 0.07116829879298564 0.013013631779288805 34-35 0.0 0.0 0.0 0.10614243419982432 0.013826983765494355 36-37 0.0 0.0 0.0 0.1472167095032046 0.013826983765494355 38-39 0.0 0.0 0.0 0.19235774473761263 0.01423365975859713 40-41 0.0 0.0 0.0 0.23831213195822626 0.016267039724111008 42-43 0.0 0.0 0.0 0.2838598431857371 0.01667371571721378 44-45 0.0 0.0 0.0 0.3139538666753424 0.017080391710316556 46-47 0.0 0.0 0.0 0.3603149298890588 0.017080391710316556 48-49 0.0 0.0 0.0 0.3944757133096919 0.017893743696522108 50-51 0.0 0.0 0.0 0.41765624491655007 0.017893743696522108 52-53 0.0 0.0 0.0 0.4729641799785275 0.019113771675830435 54-55 0.0 0.0 0.0 0.5795132901714546 0.02033379965513876 56-57 0.0 0.0 0.0 0.699889384129876 0.02033379965513876 58-59 0.0 0.0 0.0 0.8349058138399974 0.02033379965513876 60-61 0.0 0.0 0.0 0.9870026352604353 0.021553827634447083 62-63 0.0 0.0 0.0 1.0593909620327293 0.02277385561375541 64-65 0.0 0.0 0.0 1.1777336760256367 0.02277385561375541 66-67 0.0 0.0 0.0 1.4050655561700882 0.02277385561375541 68-69 0.0 0.0 0.0 1.6673715717213782 0.02277385561375541 70-71 0.0 0.0 0.0 1.9593649347691708 0.02440055958616651 72-73 0.0 0.0 0.0 2.292432573120344 0.02602726355857761 74-75 0.0 0.0 0.0 2.616960015616358 0.02602726355857761 76-77 0.0 0.0 0.0 2.9187136024986176 0.026433939551680385 78-79 0.0 0.0 0.0 3.2294140612291375 0.02684061554478316 80-81 0.0 0.0 0.0 3.5523147997527413 0.028873995510297037 82-83 0.0 0.0 0.0 3.9565507368968995 0.032534079448222016 84-85 0.0 0.0 0.0 4.53687737905456 0.03334743143442756 86-87 0.0 0.0 0.0 5.19040569997072 0.03334743143442756 88-89 0.0 0.0 0.0 5.9496697790936 0.03416078342063311 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAGGAC 20 0.0020651454 71.28914 3 CTAAGAC 20 0.0020651454 71.28914 3 GTCTAAG 25 0.0049916706 57.054565 1 CATAGGA 25 0.0049997377 57.031307 2 CTACCCC 25 0.0049997377 57.031307 4 ACCGGGC 25 0.005007815 57.008064 8 GACCGGG 25 0.005007815 57.008064 7 TATTAGG 40 5.343982E-4 47.545475 1 GTGAGTG 40 5.354744E-4 47.526093 3 GTGACGC 20 5.376096E-4 47.50672 34-35 ATTAGGG 45 9.565543E-4 42.245415 2 CCAAGAC 75 6.8460395E-6 38.02087 3 CGCCTCC 40 1.0731192E-5 35.630043 38-39 ACTTGAC 55 0.002568909 34.550343 8 TATTGGA 75 3.0527098E-4 31.68406 2 GCGGCCT 30 0.003942219 31.67115 12-13 CCATCCC 65 0.005819988 29.23491 9 TTTCGTG 35 0.008373158 27.146698 30-31 CTTGACA 70 0.008357128 27.146698 9 GTGTCAA 35 0.00838982 27.135641 58-59 >>END_MODULE