##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937808_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 122948 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11343820151609 33.0 31.0 34.0 31.0 34.0 2 32.315556170088165 34.0 31.0 34.0 31.0 34.0 3 32.40568370367961 34.0 31.0 34.0 30.0 34.0 4 36.048443244298404 37.0 35.0 37.0 35.0 37.0 5 35.88813970133715 37.0 35.0 37.0 35.0 37.0 6 36.00743403715392 37.0 35.0 37.0 35.0 37.0 7 35.969141425643365 37.0 35.0 37.0 35.0 37.0 8 35.99691739597228 37.0 35.0 37.0 35.0 37.0 9 37.61075413996161 39.0 37.0 39.0 35.0 39.0 10-11 37.57278686924553 39.0 37.0 39.0 35.0 39.0 12-13 37.500296873474966 39.0 37.0 39.0 35.0 39.0 14-15 38.61802550671828 40.0 38.0 41.0 34.0 41.0 16-17 38.59192097472102 40.0 38.0 41.0 34.5 41.0 18-19 38.59293766470378 40.0 38.0 41.0 34.0 41.0 20-21 38.52277385561376 40.0 38.0 41.0 34.0 41.0 22-23 38.36280378696685 40.0 38.0 41.0 34.0 41.0 24-25 38.25027653967531 40.0 38.0 41.0 34.0 41.0 26-27 38.06163174675473 40.0 37.0 41.0 33.5 41.0 28-29 37.9209787877802 40.0 37.0 41.0 33.0 41.0 30-31 37.92336597585971 40.0 37.0 41.0 33.0 41.0 32-33 37.724045938120184 40.0 36.0 41.0 33.0 41.0 34-35 37.48171178059017 40.0 35.5 41.0 33.0 41.0 36-37 37.25526645411068 39.5 35.0 41.0 33.0 41.0 38-39 37.02906919998699 39.0 35.0 41.0 32.5 41.0 40-41 36.78060643524091 39.0 35.0 41.0 32.0 41.0 42-43 36.45469222760842 39.0 35.0 41.0 31.0 41.0 44-45 35.99850343234538 39.0 35.0 41.0 30.0 41.0 46-47 35.407534079448226 38.5 35.0 40.0 28.0 41.0 48-49 34.837825747470475 38.0 35.0 40.0 25.5 41.0 50-51 34.40772928392491 38.0 34.0 40.0 23.5 41.0 52-53 34.11387741158864 38.0 34.0 40.0 23.0 41.0 54-55 33.8079187949377 37.5 34.0 40.0 22.0 41.0 56-57 33.38463822103654 37.0 33.0 40.0 19.5 41.0 58-59 33.06949686046133 36.0 33.0 40.0 17.5 41.0 60-61 32.55856947652666 36.0 33.0 39.0 12.0 41.0 62-63 32.14413410547549 35.0 32.0 39.0 7.5 40.5 64-65 31.69973891401243 35.0 31.5 38.5 2.0 40.0 66-67 31.181023684809837 35.0 31.0 38.0 2.0 40.0 68-69 30.696676643784365 35.0 31.0 37.0 2.0 39.5 70-71 30.21177652340827 35.0 30.5 36.5 2.0 39.0 72-73 29.754632039561443 34.0 29.5 36.0 2.0 39.0 74-75 29.300537625662884 34.0 29.0 35.5 2.0 37.5 76-77 28.13046165858737 32.5 28.0 35.0 2.0 36.0 78-79 28.65603344503367 34.0 29.0 35.0 2.0 36.5 80-81 28.449539642775807 34.0 29.0 35.0 2.0 36.0 82-83 28.121034909067248 34.0 28.5 35.0 2.0 36.0 84-85 27.762480886228325 34.0 27.5 35.0 2.0 35.0 86-87 27.38984774050818 33.5 27.0 35.0 2.0 35.0 88-89 26.9834726876403 33.0 25.5 35.0 2.0 35.0 90-91 26.526218401275337 33.0 25.0 35.0 2.0 35.0 92-93 26.163077073234213 33.0 24.5 35.0 2.0 35.0 94-95 25.701133812668772 33.0 23.0 35.0 2.0 35.0 96-97 25.158567036470703 32.0 19.5 34.0 2.0 35.0 98-99 24.44776246868595 32.0 8.5 34.0 2.0 35.0 100-101 22.298992256889093 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.486959444722288 1101 2 0.19060822034750657 1101 3 0.47448865759629655 1101 4 0.25707086955357994 1101 5 -0.053376468232436025 1101 6 0.3265090974848661 1101 7 0.10238405979217191 1101 8 0.26594485239743904 1101 9 0.2942716586499827 1101 10-11 0.3655525318645445 1101 12-13 0.4903047676566743 1101 14-15 0.7692570437542017 1101 16-17 -0.02488409201702524 1101 18-19 0.38372542705374 1101 20-21 -0.14314783208932624 1101 22-23 0.1327063351162252 1101 24-25 0.0724168999457433 1101 26-27 0.2384107194398979 1101 28-29 0.11614265719276773 1101 30-31 0.41949451836888585 1101 32-33 0.4169567315204432 1101 34-35 0.0429116984655451 1101 36-37 0.062104576337105755 1101 38-39 0.27213580445308594 1101 40-41 0.19957901872427897 1101 42-43 -0.2845549706932644 1101 44-45 0.5765541821603506 1101 46-47 0.16720249278743893 1101 48-49 0.06367716994702022 1101 50-51 0.8367221996970429 1101 52-53 1.1998037256552436 1101 54-55 1.2549229779890112 1101 56-57 1.4155287100420395 1101 58-59 1.1650697377487944 1101 60-61 1.445103941824641 1101 62-63 1.6510924784463192 1101 64-65 1.7516435548630263 1101 66-67 2.2579469095115066 1101 68-69 2.319072803853526 1101 70-71 2.297279233067499 1101 72-73 2.1091214213362406 1101 74-75 2.4828178248171113 1101 76-77 1.799712338404877 1101 78-79 2.2541848427923554 1101 80-81 1.9779832235049817 1101 82-83 2.387756705284623 1101 84-85 2.4112949062675533 1101 86-87 1.7493908475674154 1101 88-89 2.2046387665176574 1101 90-91 2.119575281170796 1101 92-93 1.8329037655497693 1101 94-95 1.689196276999791 1101 96-97 2.4416854698201753 1101 98-99 2.409234428570848 1101 100-101 2.41685464758109 1102 1 0.26147126383842334 1102 2 0.4513995872539809 1102 3 0.30429338524275096 1102 4 0.283792349512467 1102 5 0.2621939325897671 1102 6 0.2957793955321435 1102 7 0.5092699796277316 1102 8 0.17863755537791093 1102 9 0.645350795340633 1102 10-11 0.5022688730772913 1102 12-13 0.7371187450461889 1102 14-15 0.8024533438569748 1102 16-17 0.4751672954444359 1102 18-19 0.5413821588111958 1102 20-21 0.8011224659579526 1102 22-23 0.408143128538633 1102 24-25 0.41003252173402416 1102 26-27 0.051103422420375466 1102 28-29 0.2094622872030385 1102 30-31 0.1355531000749508 1102 32-33 0.3561139771525035 1102 34-35 0.33962290093214165 1102 36-37 0.6140059124111019 1102 38-39 0.17077403672440994 1102 40-41 0.11450707627824386 1102 42-43 0.6753885030991285 1102 44-45 1.2015284884296236 1102 46-47 1.2348283920680174 1102 48-49 1.2722588760107314 1102 50-51 1.2360027752366065 1102 52-53 0.7234162641958335 1102 54-55 0.7767883428399927 1102 56-57 0.8785297377912684 1102 58-59 1.1143760070190964 1102 60-61 1.2178428935204195 1102 62-63 1.0076186860516678 1102 64-65 0.7386425271137931 1102 66-67 1.4877259434272254 1102 68-69 1.3947151471217687 1102 70-71 1.180218595862982 1102 72-73 1.3434996330525806 1102 74-75 1.5426842174173174 1102 76-77 1.3586023692373566 1102 78-79 1.338023486163376 1102 80-81 1.236462154645782 1102 82-83 1.2422017207008622 1102 84-85 1.3967008672130845 1102 86-87 1.760284989396812 1102 88-89 1.5621156421599984 1102 90-91 1.3596831948398602 1102 92-93 1.8734484726412397 1102 94-95 1.2590575308538519 1102 96-97 0.7420965695118511 1102 98-99 1.4972354563200732 1102 100-101 2.155549406060018 1103 1 -0.033766831399667296 1103 2 0.05060593646032885 1103 3 0.06778544873480996 1103 4 -0.15271558699546972 1103 5 -0.11161559121975984 1103 6 -0.04707774732499814 1103 7 0.24101601137376605 1103 8 0.21752644426680234 1103 9 0.10408095407078832 1103 10-11 0.10901490482331866 1103 12-13 0.4688647767922234 1103 14-15 0.25800889941253047 1103 16-17 0.5180244383015733 1103 18-19 0.43979485722389455 1103 20-21 0.28485262468811356 1103 22-23 0.23353995393546256 1103 24-25 -0.20504684334534318 1103 26-27 -0.48461086329391634 1103 28-29 -0.12069644295569049 1103 30-31 0.1637277032495561 1103 32-33 0.2640504850890153 1103 34-35 0.35628956759881447 1103 36-37 0.018767817173007018 1103 38-39 -0.7121624712121033 1103 40-41 -0.3890643522931896 1103 42-43 -0.011516258805635005 1103 44-45 -0.40720167030053744 1103 46-47 -0.567552560312933 1103 48-49 -0.3301220763702162 1103 50-51 -0.3671718279380016 1103 52-53 -0.19285357707400408 1103 54-55 0.2922645333161853 1103 56-57 0.4737678330293704 1103 58-59 0.44493156257465216 1103 60-61 0.5730016236791542 1103 62-63 0.3941266225596003 1103 64-65 0.4711822096534739 1103 66-67 0.2047894354907207 1103 68-69 0.4486834010900189 1103 70-71 0.6587900244344098 1103 72-73 1.156198045750994 1103 74-75 1.070461995195096 1103 76-77 0.5760626866976715 1103 78-79 1.1027060258459187 1103 80-81 1.2761446943283232 1103 82-83 1.3632334667326056 1103 84-85 0.9316215021337193 1103 86-87 1.4174278465396704 1103 88-89 1.4894965945409524 1103 90-91 2.0045244646811327 1103 92-93 1.671861171053937 1103 94-95 1.8150099118062322 1103 96-97 1.9436838710991502 1103 98-99 1.3523941864788043 1103 100-101 2.0166605171711325 1104 1 0.2936141209812817 1104 2 0.20139958725398088 1104 3 0.22803288104106656 1104 4 0.43190159320995036 1104 5 0.163244352757836 1104 6 0.0831743535153393 1104 7 0.0517069544176465 1104 8 0.2635115049577408 1104 9 0.3938802071053331 1104 10-11 0.4837814781193046 1104 12-13 0.24646748454198786 1104 14-15 0.31379788167209455 1104 16-17 0.12201603494023061 1104 18-19 0.10640316721455889 1104 20-21 0.02160565923526292 1104 22-23 0.2490044730764467 1104 24-25 0.31654512677604174 1104 26-27 0.8021538425884316 1104 28-29 -0.017638553133089374 1104 30-31 -0.08598051337042278 1104 32-33 -0.009117115284468014 1104 34-35 0.111891808495173 1104 36-37 -0.08714954977376976 1104 38-39 -0.3854234422671894 1104 40-41 -0.24358115901587496 1104 42-43 0.18106077200669546 1104 44-45 -0.27998411661238976 1104 46-47 -0.4534069020496361 1104 48-49 -0.2091486870144692 1104 50-51 -0.2014972247633935 1104 52-53 -0.3106173492495401 1104 54-55 0.09180935124334866 1104 56-57 -0.22020975800704434 1104 58-59 -0.13425844676241638 1104 60-61 -0.014825173706462635 1104 62-63 0.5059380137827567 1104 64-65 0.3248820229121101 1104 66-67 0.12186459888941314 1104 68-69 0.01929497905453914 1104 70-71 0.1477606126697033 1104 72-73 0.1463357675063648 1104 74-75 0.35308337708118387 1104 76-77 0.059022537304581846 1104 78-79 0.6276243264995109 1104 80-81 0.5620924067466255 1104 82-83 0.749029451793298 1104 84-85 0.346070615112243 1104 86-87 0.27824717427075996 1104 88-89 0.45360723879865716 1104 90-91 1.0361537830751573 1104 92-93 0.5569568760025838 1104 94-95 0.8778600518622532 1104 96-97 1.0911511913605914 1104 98-99 1.5234959605217568 1104 100-101 0.4738267169843908 1105 1 -0.21879045917139095 1105 2 -0.23753171045594001 1105 3 -0.2765135940375103 1105 4 -0.08589140185067379 1105 5 -0.40023791037642553 1105 6 -0.28862627513306904 1105 7 -0.3229001403286489 1105 8 -0.2700538295730155 1105 9 -0.15770263977387344 1105 10-11 0.051641828366271625 1105 12-13 0.05571198386844145 1105 14-15 -0.20921514033059196 1105 16-17 -0.1779951909241646 1105 18-19 -0.13211838644507878 1105 20-21 -0.1094282428751967 1105 22-23 -0.5351502193348665 1105 24-25 -0.6499456680151567 1105 26-27 -0.7365192602404775 1105 28-29 -0.9984984543454871 1105 30-31 -0.7521513710264642 1105 32-33 -0.19263193342546714 1105 34-35 -0.42515354399697003 1105 36-37 -0.3739984496390605 1105 38-39 -0.40706129588191686 1105 40-41 -1.4089662061645072 1105 42-43 -1.79704879242977 1105 44-45 -2.033913169149436 1105 46-47 -1.5979960354129048 1105 48-49 -1.7692351261702939 1105 50-51 -2.3326995148397316 1105 52-53 -2.937188970264362 1105 54-55 -2.641941210049872 1105 56-57 -1.9208159546841905 1105 58-59 -1.8175487476155823 1105 60-61 -1.9019771719103282 1105 62-63 -1.168062884286389 1105 64-65 -1.3051415514031106 1105 66-67 -1.5233700216763708 1105 68-69 -1.5871823446993893 1105 70-71 -1.7744160824358204 1105 72-73 -1.9519747399027096 1105 74-75 -1.5175666004670916 1105 76-77 -1.287744413750648 1105 78-79 -1.512221879158325 1105 80-81 -1.3273328289965285 1105 82-83 -1.123528922701535 1105 84-85 -1.231107202579718 1105 86-87 -1.114742273416713 1105 88-89 -0.4966902466077201 1105 90-91 -1.0744771105036506 1105 92-93 -0.8457990737055425 1105 94-95 -0.6905335269433124 1105 96-97 -0.8938903443376596 1105 98-99 -0.5377972590561555 1105 100-101 -0.3032837455212203 1106 1 -0.13346921235205045 1106 2 0.12848292058731658 1106 3 0.0534005280998926 1106 4 -0.07483860286848198 1106 5 -0.008937019791183332 1106 6 0.08685082410357126 1106 7 0.0039128367705885125 1106 8 -0.1654100636697109 1106 9 0.15368412867396586 1106 10-11 0.026673634982046224 1106 12-13 -0.23178006447761845 1106 14-15 -0.26555260852398277 1106 16-17 -0.3562315140793828 1106 18-19 0.08587620643024252 1106 20-21 -0.054681105470621105 1106 22-23 0.2303157475862534 1106 24-25 -0.22910438302787384 1106 26-27 -0.07836086329391634 1106 28-29 -0.22015080803505072 1106 30-31 -0.30105404278219083 1106 32-33 -0.49165387999035204 1106 34-35 -0.683144956210711 1106 36-37 -0.5102500399698542 1106 38-39 -0.12684501089464106 1106 40-41 -0.4813262570550947 1106 42-43 0.1960730269086497 1106 44-45 0.24483205985819723 1106 46-47 0.0931617254013446 1106 48-49 -0.4692589811321213 1106 50-51 0.09016944190327081 1106 52-53 -0.11423254532797245 1106 54-55 -0.6005926095409606 1106 56-57 -1.128910738399199 1106 58-59 -0.7237192310761387 1106 60-61 -1.0276928207652887 1106 62-63 -0.19044679013881094 1106 64-65 -0.6622503300290639 1106 66-67 -0.8334050089537222 1106 68-69 -2.106624138592519 1106 70-71 -1.9870433088989223 1106 72-73 -2.426887271709326 1106 74-75 -1.6619288778207775 1106 76-77 -1.825475011715028 1106 78-79 -1.5813217519318634 1106 80-81 -1.7412164167827875 1106 82-83 -1.6878578031086597 1106 84-85 -1.022495561358344 1106 86-87 -1.1993876296508112 1106 88-89 -0.45693197688762055 1106 90-91 -1.088233471826804 1106 92-93 -0.5220872416444742 1106 94-95 -0.24897818343186273 1106 96-97 -1.245552240011957 1106 98-99 -0.7185383532037335 1106 100-101 0.3028708346314488 1107 1 0.30005741995035606 1107 2 0.12407999962511695 1107 3 0.2760165074813372 1107 4 0.029005898849732148 1107 5 -0.007540971681223141 1107 6 -0.089051237752102 1107 7 0.1179592285231692 1107 8 0.2800366717254832 1107 9 -0.15258734898926463 1107 10-11 -0.26735557463993587 1107 12-13 0.0648801588900767 1107 14-15 0.31880091381278675 1107 16-17 0.3792173690821343 1107 18-19 0.20824519268453656 1107 20-21 -0.1721102291819605 1107 22-23 -0.06902918368523103 1107 24-25 -0.10469501876671217 1107 26-27 -1.015538698345459 1107 28-29 -0.5356039867292139 1107 30-31 -0.4353968262873451 1107 32-33 -0.46700826143364793 1107 34-35 0.22165530152125257 1107 36-37 -0.10469806402483073 1107 38-39 -0.557686935293269 1107 40-41 -0.1537918927939259 1107 42-43 -0.3873140005827551 1107 44-45 0.536874585631395 1107 46-47 0.7173026188721394 1107 48-49 0.7393428229915884 1107 50-51 1.308167723690211 1107 52-53 1.3766931419572472 1107 54-55 1.2490637478473516 1107 56-57 1.1428890897794872 1107 58-59 1.4803185696111427 1107 60-61 1.824433467894508 1107 62-63 1.9616687596893705 1107 64-65 1.251087298836918 1107 66-67 1.5558776370943868 1107 68-69 1.2028152943971726 1107 70-71 1.671676622372555 1107 72-73 1.6901122987374109 1107 74-75 1.683700417711865 1107 76-77 1.4876156240238068 1107 78-79 1.5689145023636684 1107 80-81 1.510394407959481 1107 82-83 1.7677908223208938 1107 84-85 2.3423562427653657 1107 86-87 2.3931274047134536 1107 88-89 2.395731253352931 1107 90-91 2.0027244319532613 1107 92-93 1.416379253200887 1107 94-95 1.1089470743000973 1107 96-97 1.1384146843866638 1107 98-99 1.621130890782517 1107 100-101 2.1279352676211403 1108 1 0.20301779070604908 1108 2 0.32525279826315767 1108 3 0.2801780510356693 1108 4 0.16321567847707996 1108 5 0.2182227661415368 1108 6 0.07022238373659917 1108 7 -0.3005596402936348 1108 8 -0.07634278538224493 1108 9 0.13342418983604176 1108 10-11 0.27648250348357806 1108 12-13 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0.07311272829067406 2316 74-75 -0.6202622111541132 2316 76-77 -1.0911000117150245 2316 78-79 -0.9511134185985313 2316 80-81 -1.0432997501161232 2316 82-83 -1.2972328031086633 2316 84-85 -1.928745561358344 2316 86-87 -1.0691792963174755 2316 88-89 -1.3788069768876206 2316 90-91 -0.6715668051601398 2316 92-93 -0.6470872416444742 2316 94-95 -1.4625198500985306 2316 96-97 -0.792427240011957 2316 98-99 -2.1977050198704013 2316 100-101 -2.598170832035219 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 2.0 6 15.0 7 34.0 8 72.0 9 119.0 10 176.0 11 283.0 12 463.0 13 637.0 14 772.0 15 1077.0 16 1506.0 17 2067.0 18 1966.0 19 1259.0 20 1170.0 21 1262.0 22 1237.0 23 1169.0 24 1214.0 25 1300.0 26 1499.0 27 1626.0 28 1966.0 29 2512.0 30 3026.0 31 3832.0 32 4656.0 33 6370.0 34 8944.0 35 13334.0 36 19429.0 37 23699.0 38 13699.0 39 555.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.04935589137635 19.204446506026056 15.177852103220136 20.568345499377457 2 17.384585353157433 22.35497934085955 35.18154016332108 25.07889514266194 3 21.738458535315743 23.854800403422587 30.494192666818492 23.912548394443178 4 14.18811204736962 18.027946774246022 34.439763151901616 33.34417802648274 5 14.268283755094071 37.211742599868224 32.04161481083807 16.47835883419963 6 31.529589745258157 34.70328919543221 15.558610144125973 18.208510915183655 7 27.152129355499888 34.96681523896281 19.295962520740474 18.585092884796826 8 26.85118912060383 30.43481797182549 19.204053746299248 23.509939161271433 9 25.300126882909847 18.705468978755246 20.77219637570355 35.222207762631356 10-11 26.4506132673976 25.52135862315776 24.59535738686274 23.432670722581904 12-13 27.878659709541374 23.575056835050244 25.95053825537747 22.59574520003091 14-15 24.304584051794254 26.055324202101705 26.521781566190583 23.118310179913458 16-17 22.038992094218692 28.57996876728373 26.934964375183 22.44607476331457 18-19 21.218726616130397 27.00613267397599 30.232293327260308 21.54284738263331 20-21 23.162325382785333 25.00132170235263 29.868846458854385 21.967506456007648 22-23 22.014591534632526 25.012606955786186 30.65401633210788 22.318785177473405 24-25 23.551611670069217 25.82454228244691 30.012119043164944 20.611727004318933 26-27 22.719638216622677 24.82217875254684 31.789436868881992 20.66874616194849 28-29 20.89494574854001 27.223740503961093 30.968473964179395 20.912839783319505 30-31 21.878126347125185 25.51668605171334 30.496474090461746 22.108713510699733 32-33 22.153268048280577 26.064271073949964 30.477518951101278 21.304941926668185 34-35 23.06259556885838 25.688502456322997 29.672300484757784 21.57660149006084 36-37 22.749861730162348 25.23912548394443 30.70647753521814 21.304535250675084 38-39 22.035738686273874 25.84222598171585 30.418144256108278 21.703891075902007 40-41 21.799053258288055 26.25174870677034 30.034242118619254 21.91495591632235 42-43 22.10124739194625 26.72111211885811 28.472016040801552 22.70562444839409 44-45 22.433061131535283 25.74543709535739 28.630396590428475 23.191105182678857 46-47 23.700670202036633 27.204183882617038 28.024042684712235 21.07110323063409 48-49 23.796239060415786 26.026450206591406 28.235514201125678 21.94179653186713 50-51 22.191902267625338 27.10902170023099 28.41851839802193 22.28055763412174 52-53 23.181344958844388 28.044376484367373 26.198880827666983 22.575397729121256 54-55 22.26591729837004 27.057780525100046 26.764973810066046 23.91132836646387 56-57 23.20899892637538 27.02361974167941 26.68973875134203 23.077642580603182 58-59 24.169160946091033 26.04190389432931 26.98864560627257 22.800289553307092 60-61 22.976380258320592 27.10902170023099 28.731252236717964 21.183345804730454 62-63 21.651836548784853 26.965465074665712 28.557194911669974 22.825503464879464 64-65 21.787259654488075 27.98500178937437 27.666981162768 22.560757393369553 66-67 22.435907863487003 27.03134658554836 26.798727917493576 23.734017633471062 68-69 21.719751439633015 26.929677587272664 27.291619221134138 24.05895175196018 70-71 23.457477958161174 27.19198360282396 26.780020821810847 22.570517617204022 72-73 23.370855971630284 26.017503334743143 27.87479259524352 22.736848098383057 74-75 22.115040504928913 28.3855776425806 26.427839411783843 23.07154244070664 76-77 22.189868887659824 26.844275628721086 26.73975989849367 24.226095585125417 78-79 22.479828870742104 26.854442528548656 27.014672869831145 23.651055730878095 80-81 23.096756352279012 27.2859257572307 26.44166639554934 23.17565149494095 82-83 22.3622995087354 26.651104531997266 27.155382763444706 23.831213195822624 84-85 22.04753229007385 27.01873962976218 26.89226339590721 24.04146468425676 86-87 22.356606044831963 26.589696457038748 26.48192731886651 24.571770179262778 88-89 21.67176367244689 26.921544067410615 26.09314506946026 25.31354719068224 90-91 22.50624303505047 25.9624848499638 26.310223935837058 25.22104817914867 92-93 22.84029444060352 25.989263491805282 26.97018992232299 24.20025214526821 94-95 22.510329570224812 25.84832612161239 26.974818622507073 24.666525685655724 96-97 21.353336369847415 26.602303412824934 27.555958616650944 24.48840160067671 98-99 22.413631834726207 27.11645214420789 25.530815998698632 24.93910002236727 100-101 21.624095013422274 26.213292117465226 26.361750589766537 25.80086227934597 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 674.0 1 420.0 2 196.0 3 215.5 4 166.5 5 154.5 6 231.0 7 356.0 8 475.5 9 539.5 10 487.5 11 395.5 12 380.5 13 406.5 14 438.5 15 467.5 16 466.5 17 453.0 18 461.5 19 457.5 20 458.0 21 490.0 22 510.0 23 540.0 24 580.5 25 600.0 26 622.0 27 689.5 28 759.5 29 816.0 30 914.0 31 988.0 32 1025.0 33 1092.5 34 1259.5 35 1358.0 36 1384.0 37 1445.0 38 1604.0 39 1729.0 40 1780.5 41 2233.0 42 2905.0 43 3303.0 44 3353.0 45 3406.5 46 3620.5 47 4962.5 48 7846.0 49 7951.5 50 5588.5 51 4541.5 52 4356.0 53 4345.0 54 4288.5 55 4130.5 56 3664.5 57 3189.0 58 3077.5 59 2938.5 60 2682.0 61 2391.0 62 2074.0 63 1762.0 64 1364.0 65 1053.0 66 809.5 67 741.0 68 597.5 69 341.0 70 270.0 71 248.0 72 231.5 73 175.5 74 115.5 75 94.0 76 62.5 77 40.5 78 26.0 79 12.5 80 4.0 81 0.5 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05286787910336077 2 0.0 3 0.0 4 0.0 5 0.008946871848261054 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003660083937924976 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.0667599310277515E-4 22-23 0.0 24-25 8.133519862055503E-4 26-27 0.0012200279793083255 28-29 0.0016267039724111006 30-31 8.133519862055503E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009353547841363828 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.008946871848261054 92-93 0.002440055958616651 94-95 0.0 96-97 0.0 98-99 4.0667599310277515E-4 100-101 0.014640335751699905 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 122948.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.8591925041481 #Duplication Level Percentage of deduplicated Percentage of total 1 73.48490468066167 32.22988580538114 2 13.057265781470218 11.45362266974656 3 5.101624508567613 6.712593942154407 4 2.5554484088717455 4.4831961479649935 5 1.43720792226096 3.1517389465465078 6 0.8382167494992953 2.2058105865894526 7 0.5619019360581559 1.725119562741972 8 0.4395074549365774 1.5421153658457234 9 0.30969512647429714 1.2224680352669421 >10 1.9731473926266598 16.537885935517455 >50 0.15021140864921 4.452288772489182 >100 0.07417847340701728 7.1021895435468645 >500 0.00927230917587716 2.7897973126850375 >1k 0.007417847340701729 4.391287373523766 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1776 1.4445131275010574 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1230 1.0004229430328269 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1219 0.9914760711845658 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1174 0.9548752318053161 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 794 0.6458014770472069 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 766 0.6230276214334516 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 703 0.5717864463025019 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 615 0.5002114715164134 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 552 0.44897029638546376 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 498 0.40504928913036403 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 439 0.35706152194423657 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 433 0.3521814100270033 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 386 0.3139538666753424 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 355 0.2887399551029704 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 341 0.2773530272960927 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 323 0.26271269154439275 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 309 0.25132576373751503 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 300 0.2440055958616651 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 289 0.23505872401340405 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 275 0.22367179620652633 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 265 0.21553827634447084 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 258 0.20984481244103198 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 245 0.19927123662035984 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 222 0.18056414093763218 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 217 0.17649738100660442 No Hit GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG 216 0.17568402902039887 No Hit GATAATACGTCTCAATGAAGTCACATAAGTGGGGATCATTCTTGTCAGTA 205 0.1667371571721378 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 204 0.16592380518593225 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 196 0.15941698929628786 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 183 0.1488434134756157 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 181 0.14721670950320462 No Hit CCCTGGGACACGGTGATGAGAGCTAAGCTGACTTCCCCAAAGCCACGTGA 168 0.13664313368253245 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 168 0.13664313368253245 No Hit TCCTACGTCTATCTGTCTATGTCTTGTTATTTTGACCGAGATGATGTGGC 165 0.1342030777239158 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 161 0.13094966977909359 No Hit AGATGATGTGGCTCTGAAGAACTTTGCCAAATACTTTCTCCACCAATCTC 159 0.12932296580668248 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 155 0.1260695578618603 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 146 0.11874938998601035 No Hit GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC 132 0.10736246217913263 No Hit GTGTAGGAGGCCCGCAGGTGCAAGTTGACCAGGCGGTTCACGGCAGCTTC 130 0.10573575820672154 No Hit ACCTGGAGTTGTATGCCTCCTACGTCTATCTGTCTATGTCTTGTTATTTT 128 0.10410905423431044 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.133519862055503E-4 2 0.0 0.0 0.0 0.0 8.133519862055503E-4 3 0.0 0.0 0.0 0.0 8.133519862055503E-4 4 0.0 0.0 0.0 0.0 8.133519862055503E-4 5 0.0 0.0 0.0 0.0 8.133519862055503E-4 6 0.0 0.0 0.0 0.0 8.133519862055503E-4 7 0.0 0.0 0.0 0.0 8.133519862055503E-4 8 0.0 0.0 0.0 0.0 8.133519862055503E-4 9 0.0 0.0 0.0 0.0 8.133519862055503E-4 10-11 0.0 0.0 0.0 0.0 8.133519862055503E-4 12-13 0.0 0.0 0.0 0.0 8.133519862055503E-4 14-15 0.0 0.0 0.0 4.0667599310277515E-4 0.0012200279793083255 16-17 0.0 0.0 0.0 8.133519862055503E-4 0.0016267039724111006 18-19 0.0 0.0 0.0 8.133519862055503E-4 0.0016267039724111006 20-21 0.0 0.0 0.0 8.133519862055503E-4 0.0016267039724111006 22-23 0.0 0.0 0.0 8.133519862055503E-4 0.002440055958616651 24-25 0.0 0.0 0.0 0.0028467319517194263 0.0036600839379249766 26-27 0.0 0.0 0.0 0.008540195855158278 0.004066759931027752 28-29 0.0 0.0 0.0 0.02196050362754986 0.004066759931027752 30-31 0.0 0.0 0.0 0.049614471158538564 0.004066759931027752 32-33 0.0 0.0 0.0 0.07442170673780785 0.006506815889644402 34-35 0.0 0.0 0.0 0.10695578618602987 0.008133519862055504 36-37 0.0 0.0 0.0 0.1472167095032046 0.008133519862055504 38-39 0.0 0.0 0.0 0.19479780069622932 0.008133519862055504 40-41 0.0 0.0 0.0 0.23953215993753457 0.008133519862055504 42-43 0.0 0.0 0.0 0.28751992712366203 0.008133519862055504 44-45 0.0 0.0 0.0 0.3192406545856785 0.008133519862055504 46-47 0.0 0.0 0.0 0.3680417737580115 0.008133519862055504 48-49 0.0 0.0 0.0 0.4005758532062335 0.008133519862055504 50-51 0.0 0.0 0.0 0.4257897647786056 0.008133519862055504 52-53 0.0 0.0 0.0 0.4819110518267886 0.008540195855158278 54-55 0.0 0.0 0.0 0.5896801899990239 0.008946871848261054 56-57 0.0 0.0 0.0 0.7088362559781372 0.008946871848261054 58-59 0.0 0.0 0.0 0.8438526856882584 0.008946871848261054 60-61 0.0 0.0 0.0 0.9987962390604158 0.008946871848261054 62-63 0.0 0.0 0.0 1.070777889839607 0.008946871848261054 64-65 0.0 0.0 0.0 1.1911539837980283 0.008946871848261054 66-67 0.0 0.0 0.0 1.4172658359631713 0.008946871848261054 68-69 0.0 0.0 0.0 1.678351823535153 0.008946871848261054 70-71 0.0 0.0 0.0 1.971158538569151 0.010573575820672153 72-73 0.0 0.0 0.0 2.3054462048996323 0.013826983765494355 74-75 0.0 0.0 0.0 2.6360737872921884 0.013826983765494355 76-77 0.0 0.0 0.0 2.9451475420502975 0.013826983765494355 78-79 0.0 0.0 0.0 3.2550346487946125 0.013826983765494355 80-81 0.0 0.0 0.0 3.576308683345805 0.013826983765494355 82-83 0.0 0.0 0.0 3.984204704427888 0.013826983765494355 84-85 0.0 0.0 0.0 4.573884894426913 0.013826983765494355 86-87 0.0 0.0 0.0 5.23595341119823 0.013826983765494355 88-89 0.0 0.0 0.0 6.013924586003839 0.013826983765494355 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGAGCTA 25 0.0050108107 56.99951 5 CTCACTC 65 1.3213603E-4 36.538147 2 GCACCGT 55 0.0025708168 34.54516 6 ACTCTCA 70 2.0428209E-4 33.92828 5 CAGAGGT 60 0.0039375215 31.666395 4 CGACTAG 40 4.483707E-4 29.687244 16-17 AGTGCCC 35 0.008379317 27.142624 48-49 TCAGAGG 70 0.008363302 27.142624 3 CATGTAA 35 0.008379317 27.142624 10-11 ACTAGGA 45 8.9279155E-4 26.388662 18-19 AGAGGTG 90 8.9024194E-4 26.388662 5 ACGACTA 45 8.9279155E-4 26.388662 14-15 TCAAGCA 110 9.4098374E-5 25.908869 9 TACAAAT 130 9.9270765E-6 25.576704 2 CCTCACT 95 0.0012146082 25.020145 1 CCCAGTC 95 0.0012203912 24.999786 5 GAGTACT 875 0.0 24.971214 12-13 TTGTATG 195 1.5410478E-8 24.358767 9 GTTGTAT 195 1.5410478E-8 24.358767 8 CTGAGCT 100 0.0016453675 23.749796 4 >>END_MODULE