FastQCFastQC Report
Thu 26 May 2016
SRR937807_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937807_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232707
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102980.8353972192905533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82960.6729904186477403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50450.40926189272876684No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20830.16897770516432534No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA16870.13685328305915354No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14940.12119668339678448No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT14680.11908750416765702No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA14510.11770842544091986No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC14290.11592373532396587No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA14090.11430128976309861No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA13270.10764926296354282No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG13020.10562120601245875No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA12830.10407988272963485No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12630.1024574371687676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53450.047.0595781
GTATCAA92650.039.979021
GCGTAAG753.0600652E-431.6992381
GTCTACG600.0039367131.6902122
TATCAAC116650.031.663042
ATCAACG119750.030.8421173
TCAACGC120150.030.6603164
CAACGCA121950.030.1687875
AACGCAG124650.029.5723296
GTGTAAG4050.029.3511471
TAAGGTA3950.027.6776644
AGGTAAG5350.027.5438292
ACGCAGA139850.026.2573437
CGCAGAG139950.026.1356878
TAAGGTG6750.024.6469385
GCAGAGT145300.024.6175849
GAGTACT79450.024.42286512-13
GTACATG87850.023.9773981
TACATGG87700.023.9573652
GTACTTT82650.023.39106414-15
Produced by FastQC (version 0.11.5)