FastQCFastQC Report
Thu 26 May 2016
SRR937807_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937807_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232707
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112230.910435326480664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96480.7826677385623673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63910.5184524789751336No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC26090.21164802341513433No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25440.20637507534231572No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23320.18917715239712274No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA18670.15145529310695893No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA18130.1470746900926173No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16540.13417624788372257No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA16320.13239155776676859No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA15270.12387371857221546No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14530.11787066999700659No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT12580.10205182577855078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61450.045.5515481
AGGTAAG7450.040.8173942
TAAGGTG9300.039.3395465
GGTAAGG9100.037.5934943
GTATCAA99850.036.2198221
GTGTAAG4350.032.7749251
AAGGTAA9050.032.5576321
GTAAGGT11000.031.1000734
TATCAAC128450.028.1126042
GGTGTGC13900.027.6823968
ATCAACG132200.027.2432793
TCAACGC132650.027.1508564
GTGTGCA14550.026.7722199
CAACGCA135300.026.6190765
AGGTGTG14900.026.462167
TAAGGTA3600.026.3966674
AACGCAG139050.025.9258986
AAGGTGT14150.025.8462356
TAGACTC5550.023.1149185
ACGCAGA157950.022.7070547
Produced by FastQC (version 0.11.5)