##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937807_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1232707 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11303334855728 33.0 31.0 34.0 31.0 34.0 2 32.38471672506119 34.0 31.0 34.0 31.0 34.0 3 32.453634967595704 34.0 31.0 34.0 31.0 34.0 4 36.117002661621946 37.0 35.0 37.0 35.0 37.0 5 36.03004363567336 37.0 35.0 37.0 35.0 37.0 6 36.085284662129766 37.0 35.0 37.0 35.0 37.0 7 36.030124757951405 37.0 35.0 37.0 35.0 37.0 8 36.00542789162388 37.0 35.0 37.0 35.0 37.0 9 37.67666201295198 39.0 37.0 39.0 35.0 39.0 10-11 37.5992555408544 39.0 37.0 39.0 35.0 39.0 12-13 37.49008117906364 39.0 37.0 39.0 35.0 39.0 14-15 38.6427095003111 40.0 38.0 41.0 34.0 41.0 16-17 38.60971788105365 40.0 38.0 41.0 34.0 41.0 18-19 38.64733712066209 40.0 38.0 41.0 34.0 41.0 20-21 38.57212257251723 40.0 38.0 41.0 34.0 41.0 22-23 38.511395246396745 40.0 38.0 41.0 34.0 41.0 24-25 38.49280120904643 40.0 38.0 41.0 34.0 41.0 26-27 38.343280276659414 40.0 38.0 41.0 34.0 41.0 28-29 38.17269026621898 40.0 38.0 41.0 34.0 41.0 30-31 38.11513603800417 40.0 37.5 41.0 34.0 41.0 32-33 37.942537034347986 40.0 37.0 41.0 33.0 41.0 34-35 37.763271807493595 40.0 36.5 41.0 33.0 41.0 36-37 37.565884674947085 40.0 36.0 41.0 33.0 41.0 38-39 37.37645442104247 40.0 36.0 41.0 33.0 41.0 40-41 37.13920501789963 40.0 36.0 41.0 32.0 41.0 42-43 36.833536274232245 39.5 35.0 41.0 31.0 41.0 44-45 36.41102386860787 39.0 35.0 41.0 30.5 41.0 46-47 35.86739914675588 39.0 35.0 41.0 29.5 41.0 48-49 35.37602650102579 39.0 35.0 40.5 27.0 41.0 50-51 35.02443200208971 39.0 35.0 40.0 26.0 41.0 52-53 34.7234780852222 38.0 34.5 40.0 24.5 41.0 54-55 34.446424008300426 38.0 34.0 40.0 24.0 41.0 56-57 34.11958600056623 38.0 34.0 40.0 23.0 41.0 58-59 33.80575595011629 37.0 33.5 40.0 22.5 41.0 60-61 33.43108946408189 37.0 33.0 40.0 21.5 41.0 62-63 33.019831963313266 36.0 33.0 39.0 20.0 41.0 64-65 32.57742796950127 35.5 32.0 39.0 18.0 40.0 66-67 32.13628664394702 35.0 32.0 38.5 17.0 40.0 68-69 31.667541840842958 35.0 31.0 38.0 12.5 40.0 70-71 31.253153831364628 35.0 31.0 37.0 9.0 39.5 72-73 30.779353487892905 35.0 30.5 36.5 7.0 39.0 74-75 30.33000623830318 34.0 30.0 36.0 6.0 39.0 76-77 29.158204666640167 33.5 28.5 35.0 2.0 37.0 78-79 29.727663183546454 34.0 30.0 35.0 2.0 37.0 80-81 29.564892143875227 34.0 30.0 35.0 2.0 37.0 82-83 29.1994768424289 34.0 29.0 35.0 2.0 36.5 84-85 28.845802773895173 34.0 29.0 35.0 2.0 36.0 86-87 28.50786034313101 34.0 29.0 35.0 2.0 36.0 88-89 28.068774250490993 34.0 28.0 35.0 2.0 35.0 90-91 27.52925958885607 33.0 27.0 35.0 2.0 35.0 92-93 27.087581233821176 33.0 26.0 35.0 2.0 35.0 94-95 26.615139282895285 33.0 25.0 35.0 2.0 35.0 96-97 26.041823401668037 32.5 24.5 34.5 2.0 35.0 98-99 25.143943775771533 32.0 20.5 34.0 2.0 35.0 100-101 22.472925845314418 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2160727892091927 1101 2 0.17300891099417015 1101 3 0.22565509993165023 1101 4 0.16624598763330312 1101 5 0.052835778251456134 1101 6 0.098355009971975 1101 7 0.10865237419153573 1101 8 0.03029825065093661 1101 9 0.1215336372300726 1101 10-11 0.19048014281018055 1101 12-13 0.22837722112939218 1101 14-15 0.2796988971173917 1101 16-17 0.20161711322915465 1101 18-19 0.2246154578332593 1101 20-21 0.17962547169959464 1101 22-23 0.1786867877015652 1101 24-25 0.2070780425883072 1101 26-27 0.1570537057178072 1101 28-29 0.023491008187079387 1101 30-31 0.2694583636335537 1101 32-33 0.29583079362329556 1101 34-35 0.26151112434901336 1101 36-37 0.06011679915246759 1101 38-39 0.12047881814354611 1101 40-41 0.2733117015429585 1101 42-43 0.3934210530653601 1101 44-45 0.16998181011791758 1101 46-47 0.1698051493784689 1101 48-49 0.20830927257762966 1101 50-51 0.1909415885793706 1101 52-53 0.19125377557380574 1101 54-55 0.11068291585535661 1101 56-57 0.14008495118147124 1101 58-59 4.126554558041562E-4 1101 60-61 0.12513620726291208 1101 62-63 -0.05828489307100426 1101 64-65 -0.0931540885972808 1101 66-67 -0.15101429889560336 1101 68-69 0.1565277689130653 1101 70-71 -0.02991437967824595 1101 72-73 -0.0017540280576184841 1101 74-75 -0.026366329599319727 1101 76-77 0.04851651149534675 1101 78-79 -0.043339898752307704 1101 80-81 0.05904521110302241 1101 82-83 0.053381939913634824 1101 84-85 -0.03194129712956695 1101 86-87 -0.19745976739394777 1101 88-89 -0.06140686287137953 1101 90-91 -0.017397459054659947 1101 92-93 -0.15291724226163694 1101 94-95 -0.31750193721664743 1101 96-97 -0.49681927725877273 1101 98-99 -0.6958353114847142 1101 100-101 -0.43092923829673424 1102 1 0.13669121269493445 1102 2 0.1176174782024404 1102 3 0.05982389982293057 1102 4 0.001043058376154704 1102 5 -0.04482469259522048 1102 6 0.04575514943919501 1102 7 0.06119675974487393 1102 8 0.12465472974979264 1102 9 0.15572204273657775 1102 10-11 0.07036734368529807 1102 12-13 0.17883516819868817 1102 14-15 0.12309180478607828 1102 16-17 0.11819466346297958 1102 18-19 0.15900069926080818 1102 20-21 0.12330889893937069 1102 22-23 0.12151246927518855 1102 24-25 0.0869713702181869 1102 26-27 0.0244874420802077 1102 28-29 -0.032718624853728784 1102 30-31 0.07595818205881955 1102 32-33 0.13806574464267385 1102 34-35 0.05679619667814251 1102 36-37 0.013888763568282059 1102 38-39 -0.01594395912552926 1102 40-41 -0.05511354425130577 1102 42-43 -0.0979708564992805 1102 44-45 -0.07232409986112032 1102 46-47 -0.22035494829314217 1102 48-49 -0.22774244614416972 1102 50-51 -0.48780648149288197 1102 52-53 -0.2304100950647765 1102 54-55 -0.3036438874713596 1102 56-57 -0.2409262307029394 1102 58-59 -0.2918385593125734 1102 60-61 -0.23619249647173035 1102 62-63 -0.3155400846601992 1102 64-65 -0.5093161334629457 1102 66-67 -0.5470092950079 1102 68-69 -0.2572192206224386 1102 70-71 -0.2597027727084509 1102 72-73 -0.5775642635255132 1102 74-75 -0.3219276628702694 1102 76-77 -0.506699473319216 1102 78-79 -0.3481710119725072 1102 80-81 -0.33298020490362745 1102 82-83 -0.3223290421271763 1102 84-85 -0.3078273929609274 1102 86-87 -0.7087753264547523 1102 88-89 -0.6728441772585576 1102 90-91 -0.5971527676091704 1102 92-93 -0.5356077119275149 1102 94-95 -0.6584112812974219 1102 96-97 -0.8093650886750332 1102 98-99 -0.9009607317243926 1102 100-101 -1.0392563891775382 1103 1 0.28934901241155586 1103 2 0.2936081541931159 1103 3 0.21922332392823307 1103 4 0.1911111602089619 1103 5 0.21366518354171404 1103 6 0.22156300171763377 1103 7 0.19548077638182804 1103 8 0.22507065369512702 1103 9 0.271837952970138 1103 10-11 0.29979967605881797 1103 12-13 0.3247970494546877 1103 14-15 0.32074709067666163 1103 16-17 0.2639737210067423 1103 18-19 0.3956431417856052 1103 20-21 0.2439839754967963 1103 22-23 0.2782266701070313 1103 24-25 0.30282904195821914 1103 26-27 0.328397584225641 1103 28-29 0.3100301296597294 1103 30-31 0.3356021328792451 1103 32-33 0.24515473408460764 1103 34-35 0.2629185057416237 1103 36-37 0.1318786168522479 1103 38-39 0.26441620358757945 1103 40-41 0.1482435561646227 1103 42-43 0.34730772113223907 1103 44-45 0.3321962164238954 1103 46-47 0.2799421402392426 1103 48-49 0.12205644777237268 1103 50-51 -0.01720681971675475 1103 52-53 0.005329655380855058 1103 54-55 -0.16489211636664436 1103 56-57 -0.13542552226105187 1103 58-59 -0.09828538046527768 1103 60-61 0.10119723797681957 1103 62-63 -0.02175380557979878 1103 64-65 0.05541488715042675 1103 66-67 -0.03827946274865468 1103 68-69 -0.12767719402158662 1103 70-71 -0.10926171050268252 1103 72-73 -0.219401184774199 1103 74-75 -0.22733924475243938 1103 76-77 -0.2874252834567912 1103 78-79 -0.06574265601473783 1103 80-81 0.12555309186496189 1103 82-83 0.04840316684032686 1103 84-85 -0.016977719758312304 1103 86-87 -0.041651600507496056 1103 88-89 0.07632168955436569 1103 90-91 0.02050571769637699 1103 92-93 0.011446541009089373 1103 94-95 -0.10466110990019217 1103 96-97 -0.19717988825453858 1103 98-99 0.028183653302345846 1103 100-101 0.07493987207754671 1104 1 0.1922591772643969 1104 2 0.12319072661008335 1104 3 0.10931545048532598 1104 4 0.017992170210995084 1104 5 -0.022994034805400076 1104 6 0.04213211766891334 1104 7 0.014909136415283797 1104 8 0.06048011330840808 1104 9 -0.03279089305998184 1104 10-11 0.011584043496050356 1104 12-13 0.033771594018404016 1104 14-15 0.08299227648873142 1104 16-17 0.05558718539090535 1104 18-19 0.12687015963683024 1104 20-21 0.01273437760707452 1104 22-23 0.06714537701937218 1104 24-25 0.03994821259649228 1104 26-27 0.0856046376110342 1104 28-29 0.10898550902912518 1104 30-31 0.09128611627023275 1104 32-33 0.08867506725462704 1104 34-35 0.09305413444413091 1104 36-37 -0.01953631640981257 1104 38-39 0.11200898589373054 1104 40-41 0.134600263858907 1104 42-43 0.1726409158921811 1104 44-45 0.11799795007595293 1104 46-47 0.01575816427182275 1104 48-49 -0.10177514349553007 1104 50-51 -0.15923340601084846 1104 52-53 0.009970317266741802 1104 54-55 0.059814528722164084 1104 56-57 0.06960493913883425 1104 58-59 0.05886178942075304 1104 60-61 0.036221785262512185 1104 62-63 0.09882390934504315 1104 64-65 0.09382198606707703 1104 66-67 0.09933380862257479 1104 68-69 0.06182253130616289 1104 70-71 -0.13994458035752544 1104 72-73 0.061275189453127155 1104 74-75 -0.1706339631267788 1104 76-77 -0.17004032411323067 1104 78-79 -0.09029148828650335 1104 80-81 -0.15105825680052476 1104 82-83 -0.12490203114640153 1104 84-85 -0.10291054135992184 1104 86-87 -0.06968549159871529 1104 88-89 -0.30896305401063273 1104 90-91 -0.05871464615774613 1104 92-93 -0.049865068471309115 1104 94-95 -0.1993970533622509 1104 96-97 -0.1978743017674205 1104 98-99 -0.1546227918448615 1104 100-101 -0.4333148332312966 1105 1 0.2740351248260495 1105 2 0.21422216696557683 1105 3 0.21340241349940214 1105 4 0.07180919207918635 1105 5 0.042694862207611095 1105 6 0.1694530708688191 1105 7 0.13130629102706592 1105 8 0.08257486689861082 1105 9 0.22607970511003117 1105 10-11 0.12450717012766432 1105 12-13 0.3026336833139567 1105 14-15 0.3152446437837497 1105 16-17 0.19402888994630274 1105 18-19 0.23985668446317732 1105 20-21 0.09879726604170003 1105 22-23 0.05885083351211762 1105 24-25 0.09347978167928517 1105 26-27 0.07896745807764916 1105 28-29 0.12044288227134814 1105 30-31 0.08200873983556534 1105 32-33 0.05446385665160847 1105 34-35 0.10087238694530498 1105 36-37 0.002693600844274613 1105 38-39 -0.0873368219730466 1105 40-41 0.06562174072146121 1105 42-43 0.11671405359435028 1105 44-45 -0.061867233823861056 1105 46-47 -0.23860569116227026 1105 48-49 -0.22653989435671207 1105 50-51 -0.254572705425403 1105 52-53 -0.030633169183424513 1105 54-55 -0.18220566601649324 1105 56-57 -0.12877798853187272 1105 58-59 -0.08406413059325502 1105 60-61 -0.10450225430310667 1105 62-63 -0.13475402731081942 1105 64-65 -0.07325317522822417 1105 66-67 -0.24273839194485447 1105 68-69 -0.22377121048747384 1105 70-71 -0.3223653229595662 1105 72-73 -0.24044647380655348 1105 74-75 -0.21408940288497647 1105 76-77 -0.25699851091198767 1105 78-79 -0.17945863553508445 1105 80-81 -0.23783107628880984 1105 82-83 -0.36710694955903733 1105 84-85 -0.3195328400175548 1105 86-87 -0.47750129828120436 1105 88-89 -0.4493878026062177 1105 90-91 -0.05126101373082648 1105 92-93 -0.0866672377837503 1105 94-95 -0.3947729113296994 1105 96-97 -0.4485452928131508 1105 98-99 -0.2237850732015474 1105 100-101 -0.01695507389985451 1106 1 0.0953930145136539 1106 2 0.028405859945181078 1106 3 -0.01174660517447279 1106 4 -0.003250264442918649 1106 5 -0.11847927412141246 1106 6 -0.13073108501628639 1106 7 0.011912730780586855 1106 8 -0.07182424485952765 1106 9 -0.057845733560021984 1106 10-11 -0.11798654878135295 1106 12-13 -0.2193916456676135 1106 14-15 -0.1669812943546276 1106 16-17 0.041047845591236864 1106 18-19 0.027704660257917624 1106 20-21 0.07127661139939079 1106 22-23 -0.010410983157179032 1106 24-25 -0.1633266902763566 1106 26-27 -0.05090856375270647 1106 28-29 0.06093730224739602 1106 30-31 0.10356906682127942 1106 32-33 0.07514994634122729 1106 34-35 -0.30266632436602947 1106 36-37 0.2946171577028238 1106 38-39 0.17798937545950366 1106 40-41 0.05265996732376976 1106 42-43 0.16922825104031602 1106 44-45 0.27597327845822406 1106 46-47 0.34562046836570204 1106 48-49 0.22643368648570572 1106 50-51 0.3365114997692018 1106 52-53 0.20465574422976118 1106 54-55 0.3200426123440181 1106 56-57 0.5538696800009504 1106 58-59 0.4335116030951909 1106 60-61 0.12630226204450423 1106 62-63 -0.05687520792112366 1106 64-65 -0.16691816246477487 1106 66-67 -0.15019377020238522 1106 68-69 0.26240661566802714 1106 70-71 0.10680132973048373 1106 72-73 -0.0659399042382276 1106 74-75 -0.22022332010539358 1106 76-77 -0.18634546194128276 1106 78-79 -0.3371811069137678 1106 80-81 -0.4111690610397609 1106 82-83 -0.39542427128171553 1106 84-85 -0.6363698536732656 1106 86-87 -0.5778587082913695 1106 88-89 -0.38482464451727694 1106 90-91 -0.4993795376660586 1106 92-93 -0.4502849616975162 1106 94-95 -0.2669631923267559 1106 96-97 -0.5603914807192396 1106 98-99 -0.5546660695888725 1106 100-101 -1.186164226682152 1107 1 0.15046567352821683 1107 2 0.1301715907565537 1107 3 0.006436111141020717 1107 4 -0.02038845129064981 1107 5 0.08641465629118983 1107 6 0.07093585109048206 1107 7 0.1648891457901982 1107 8 0.040632869257343884 1107 9 0.11610479723697864 1107 10-11 0.09393887019800928 1107 12-13 0.0706511953088409 1107 14-15 -0.029269559339986984 1107 16-17 0.13202788906090746 1107 18-19 0.2164667626092296 1107 20-21 0.17702870854153474 1107 22-23 0.1552385471189126 1107 24-25 0.06922930835848007 1107 26-27 0.15276210859016714 1107 28-29 0.20262642225602434 1107 30-31 0.13707843435554423 1107 32-33 0.16366955259942984 1107 34-35 0.22332476614788987 1107 36-37 0.22445270942634465 1107 38-39 0.13063886981024098 1107 40-41 0.1796232875443522 1107 42-43 0.2029742767987912 1107 44-45 0.2056116898393725 1107 46-47 0.02318778348487882 1107 48-49 -0.1355748098588876 1107 50-51 0.09652389401396277 1107 52-53 -0.3010972510460519 1107 54-55 -0.409758361232889 1107 56-57 -0.39694178377731504 1107 58-59 -0.4590801412600385 1107 60-61 -0.3087150719354881 1107 62-63 -0.20063048445648235 1107 64-65 -0.19345846172291914 1107 66-67 -0.3429525674217615 1107 68-69 -0.21485668120107704 1107 70-71 -0.15136405260502528 1107 72-73 -0.09341605878907444 1107 74-75 -0.03635245376564811 1107 76-77 -0.10035679638830075 1107 78-79 -0.20730109757317905 1107 80-81 -0.2638463975345253 1107 82-83 -0.24330512486796252 1107 84-85 -0.05215365493424784 1107 86-87 -0.1390763979322962 1107 88-89 -0.14866443543175833 1107 90-91 -0.45653945934880014 1107 92-93 -0.3531666236040749 1107 94-95 -0.18491419015327182 1107 96-97 -0.12995822103287225 1107 98-99 -0.18590226078356764 1107 100-101 -0.15421985708218244 1108 1 0.20280816405596624 1108 2 0.09682352735178057 1108 3 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-0.21392642930225136 2316 72-73 -0.20497144085623376 2316 74-75 -0.271679731416242 2316 76-77 -0.18080102045559698 2316 78-79 -0.26002914396491406 2316 80-81 -0.3800740096191646 2316 82-83 -0.3290658304499523 2316 84-85 -0.6468006001904492 2316 86-87 -0.5276659021188728 2316 88-89 -0.37397803328800805 2316 90-91 -0.09232003404082789 2316 92-93 -0.27668567157478563 2316 94-95 -0.2083755649550234 2316 96-97 -0.2200001044135007 2316 98-99 -0.27681029669566826 2316 100-101 0.1956101125690708 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 10.0 5 25.0 6 73.0 7 168.0 8 420.0 9 820.0 10 1427.0 11 2343.0 12 3726.0 13 5066.0 14 6524.0 15 9687.0 16 13848.0 17 19672.0 18 17264.0 19 9994.0 20 7980.0 21 7792.0 22 7829.0 23 8349.0 24 9701.0 25 11022.0 26 13268.0 27 16059.0 28 19736.0 29 24339.0 30 30495.0 31 37627.0 32 48021.0 33 63409.0 34 88460.0 35 129216.0 36 193335.0 37 256161.0 38 158224.0 39 10608.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.17922533554243 19.198170845497135 15.724935536583901 20.89766828237654 2 16.047771287094175 20.977166512399133 39.19593220448979 23.779129996016895 3 19.739321671735457 25.467852458045588 30.179596611360203 24.613229258858755 4 13.285801086551793 17.13091594352916 35.752291501549024 33.83099146837002 5 13.285757064012913 38.281232882159465 32.83582210673499 15.597187947092635 6 31.39302364633283 36.433313025723066 16.31920642942727 15.854456898516842 7 28.004383847905462 34.93895954188627 19.24918086779746 17.80747574241081 8 28.180256946703473 33.27846763261667 18.70671619452149 19.834559226158365 9 27.940378370529253 16.106179327285396 19.568072542785917 36.38536975939943 10-11 25.72176518832131 27.00662038911112 25.10182873951393 22.169785683053636 12-13 27.203689581879836 24.7128973115052 26.46362338357698 21.619789723037982 14-15 23.664990950809884 25.349170565268146 26.16704537250133 24.818793111420636 16-17 21.619938882475722 28.627930238085774 27.087986033988614 22.66414484544989 18-19 21.77520692265072 25.717830757836207 31.350515572638106 21.15644674687497 20-21 22.641957902405032 24.50720244145608 31.91577560604426 20.935064050094628 22-23 22.493650112476768 24.624259450198306 31.91216996201019 20.969920475314733 24-25 22.051779601859486 24.95271579464844 31.90664761372445 21.088856989767628 26-27 21.142413284686633 25.33184337601074 31.78585987235311 21.739883466949514 28-29 21.436898118515742 25.60471064665526 31.71363279053977 21.24475844428923 30-31 21.42638112706426 25.46910985335526 31.296731502295355 21.80777751728513 32-33 21.10347389931265 25.3677881280791 31.547399341449346 21.981338631158906 34-35 21.832965984617594 25.47276035586721 31.29758328621481 21.396690373300387 36-37 22.280842081695003 24.906973027653773 31.110515313046815 21.70166957760441 38-39 21.64812887409579 25.405672231925347 30.98518139346982 21.96101750050904 40-41 21.935948626508683 25.38423847527943 31.146882691462142 21.532930206749747 42-43 21.920251053085916 25.453159582270512 30.498114505649994 22.12847485899358 44-45 21.80250456921231 25.817611159829546 30.421422122207463 21.958462148750677 46-47 22.57900701464338 26.35894012121291 29.224219542843517 21.837833321300195 48-49 22.345780465268714 26.55002364714405 28.70021018782241 22.403985699764828 50-51 22.555481554010807 26.96082686315564 28.532692683662862 21.95099889917069 52-53 22.995691595813117 26.65256220659086 28.4210684290752 21.930677768520823 54-55 22.408668409174613 27.179919623981714 28.310318924382617 22.101093042461056 56-57 22.555197626037653 27.32612048118491 27.947517130997067 22.171164761780375 58-59 22.411205582510686 26.714985799545225 27.929224057298285 22.944584560645797 60-61 22.127277609358913 27.180830481209238 28.437657934935068 22.254233974496778 62-63 22.36691281869901 27.181885070823803 28.58854537209572 21.862656738381464 64-65 22.174855825431347 27.083159258445033 28.52291744915864 22.21906746696498 66-67 22.00453960267931 27.298457784372115 28.41960822807042 22.277394384878157 68-69 22.762140557326273 27.06093175426115 28.171576862952836 22.00535082545974 70-71 22.613118932560617 27.327580682189684 27.66488711429399 22.39441327095571 72-73 22.381312023051706 27.48406555653533 27.56697252469565 22.567649895717313 74-75 22.44150475335988 27.936078889792952 27.35986734885094 22.262549007996224 76-77 22.50802502135544 27.266779534796182 28.047216410712362 22.177979033136015 78-79 22.424347391553713 27.55784626841577 27.711329618473812 22.306476721556702 80-81 22.677205532214874 27.832769668704728 27.338856678837715 22.151168120242684 82-83 22.572633597632503 27.93268240130965 27.29416221706279 22.200521783995057 84-85 22.443722365155345 28.05353752376379 27.243855081363716 22.258885029717153 86-87 22.680328739919542 28.07970588306873 26.80929044777064 22.430674929241093 88-89 22.284005850538694 28.039347549742153 26.736523764365742 22.940122835353414 90-91 22.18109728666023 28.09262197291244 26.97571758257316 22.75056315785417 92-93 22.354638569757423 27.62471545876241 27.308576555586743 22.712069415893424 94-95 21.999753388274748 27.46666482789503 27.55930646942055 22.97427531440967 96-97 22.38382681367105 27.064663379051147 27.635845338754468 22.91566446852334 98-99 21.944757333259783 27.405788242528423 27.184321099775005 23.465133324436792 100-101 21.774773934174174 27.46007083193035 27.755366147530008 23.009789086365462 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 986.0 1 678.5 2 466.5 3 610.5 4 573.0 5 547.0 6 1014.5 7 2204.5 8 3415.5 9 3966.5 10 3565.0 11 2916.0 12 2811.0 13 2869.0 14 3024.0 15 3116.5 16 3203.5 17 3270.5 18 3378.0 19 3555.0 20 3753.5 21 4125.0 22 4595.5 23 5247.0 24 6035.5 25 7009.5 26 8296.0 27 9656.0 28 10973.5 29 12767.0 30 14785.5 31 16816.5 32 20484.0 33 22172.5 34 21589.5 35 22577.0 36 24771.0 37 26972.5 38 28845.5 39 30352.0 40 31660.0 41 33324.0 42 33965.0 43 35070.0 44 36611.5 45 38259.5 46 39180.0 47 41079.0 48 44540.0 49 43406.0 50 41335.0 51 41001.5 52 39788.0 53 40637.5 54 43760.0 55 43199.0 56 39618.5 57 37134.5 58 35567.5 59 31980.5 60 25854.0 61 21847.0 62 18962.5 63 13527.5 64 8601.0 65 6329.0 66 4671.0 67 3653.5 68 2878.0 69 2162.5 70 1631.0 71 1052.0 72 712.5 73 591.5 74 488.0 75 352.5 76 256.5 77 183.5 78 106.0 79 70.0 80 51.0 81 36.5 82 27.5 83 19.0 84 9.5 85 5.5 86 4.5 87 2.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018252512559756698 2 0.0 3 0.0 4 0.0 5 0.03569380233907977 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003812747068038066 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.489782243469048E-4 24-25 1.2168341706504466E-4 26-27 7.706616414119494E-4 28-29 8.11222780433631E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.301005023902679E-4 42-43 0.011275996648027472 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 8.11222780433631E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0010545896145637203 84-85 1.2168341706504466E-4 86-87 0.0 88-89 0.0 90-91 0.01204665828943942 92-93 0.0017035678389106252 94-95 0.0 96-97 0.0 98-99 0.0015007621438022174 100-101 0.01719792294519298 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1232707.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.359987653509215 #Duplication Level Percentage of deduplicated Percentage of total 1 68.97237737157623 30.596138086363002 2 15.220629840771021 13.50373903630473 3 6.023962478169695 8.016687034704315 4 3.094100981056571 5.49017125273521 5 1.779243894833864 3.946361860370593 6 1.1501756199019746 3.061306577893134 7 0.7756546529764107 2.4085621580594387 8 0.5258903309928015 1.866279087195244 9 0.3752038114783728 1.4979632800257194 >10 1.8967053109600274 14.815531152526315 >50 0.11555276473446774 3.5445013638358107 >100 0.06022126909192101 4.9165420516897465 >500 0.006609647222195358 1.9683753083655466 >1k 0.00312122229937003 2.1474740051172776 >5k 3.6720262345529765E-4 1.3063046826744042 >10k+ 1.8360131172764883E-4 0.9140630621394625 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11223 0.910435326480664 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9648 0.7826677385623673 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6391 0.5184524789751336 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2609 0.21164802341513433 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2544 0.20637507534231572 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2332 0.18917715239712274 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1867 0.15145529310695893 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1813 0.1470746900926173 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1654 0.13417624788372257 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1632 0.13239155776676859 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 1527 0.12387371857221546 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1453 0.11787066999700659 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 1258 0.10205182577855078 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.2168341706504464E-4 0.0 24-25 0.0 0.0 0.0 3.244891121734524E-4 0.0 26-27 0.0 0.0 0.0 7.706616414119494E-4 0.0 28-29 0.0 0.0 0.0 0.0023931072022792114 0.0 30-31 0.0 0.0 0.0 0.005719120602057098 0.0 32-33 0.0 0.0 0.0 0.011357118926070834 0.0 34-35 0.0 0.0 0.0 0.015940527635520847 0.0 36-37 0.0 0.0 0.0 0.01938822445236378 0.0 38-39 0.0 0.0 0.0 0.02332265493746689 0.0 40-41 0.0 0.0 0.0 0.026810912893331505 0.0 42-43 0.0 0.0 0.0 0.031069832490608067 0.0 44-45 0.0 0.0 0.0 0.03549099664397136 0.0 46-47 0.0 0.0 0.0 0.040845066994833326 0.0 48-49 0.0 0.0 0.0 0.04952515074547317 4.056113902168155E-5 50-51 0.0 0.0 0.0 0.060273852586218785 8.11222780433631E-5 52-53 0.0 0.0 0.0 0.08315033499444718 8.11222780433631E-5 54-55 0.0 0.0 0.0 0.1116648157266893 8.11222780433631E-5 56-57 0.0 0.0 0.0 0.14610122275609694 8.11222780433631E-5 58-59 0.0 0.0 0.0 0.1917325041554887 8.11222780433631E-5 60-61 0.0 0.0 0.0 0.2546428307781168 8.11222780433631E-5 62-63 0.0 0.0 0.0 0.29954401167511824 8.11222780433631E-5 64-65 0.0 0.0 0.0 0.34712222774755075 8.11222780433631E-5 66-67 0.0 0.0 0.0 0.42824450579091383 8.11222780433631E-5 68-69 0.0 0.0 0.0 0.5309453097938115 8.11222780433631E-5 70-71 0.0 0.0 0.0 0.6225323617047684 8.11222780433631E-5 72-73 0.0 0.0 0.0 0.723326792173647 8.11222780433631E-5 74-75 0.0 0.0 0.0 0.8753905023659312 8.11222780433631E-5 76-77 0.0 0.0 0.0 1.002022378391621 8.11222780433631E-5 78-79 0.0 8.11222780433631E-5 0.0 1.1177838691595001 8.11222780433631E-5 80-81 0.0 1.622445560867262E-4 0.0 1.2581254101745183 8.11222780433631E-5 82-83 0.0 1.622445560867262E-4 0.0 1.4802382074572464 8.11222780433631E-5 84-85 0.0 1.622445560867262E-4 0.0 1.7061637518080128 8.11222780433631E-5 86-87 0.0 1.622445560867262E-4 0.0 1.9798297567872982 8.11222780433631E-5 88-89 0.0 1.622445560867262E-4 0.0 2.321962964435182 8.11222780433631E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6145 0.0 45.551548 1 AGGTAAG 745 0.0 40.817394 2 TAAGGTG 930 0.0 39.339546 5 GGTAAGG 910 0.0 37.593494 3 GTATCAA 9985 0.0 36.219822 1 GTGTAAG 435 0.0 32.774925 1 AAGGTAA 905 0.0 32.557632 1 GTAAGGT 1100 0.0 31.100073 4 TATCAAC 12845 0.0 28.112604 2 GGTGTGC 1390 0.0 27.682396 8 ATCAACG 13220 0.0 27.243279 3 TCAACGC 13265 0.0 27.150856 4 GTGTGCA 1455 0.0 26.772219 9 CAACGCA 13530 0.0 26.619076 5 AGGTGTG 1490 0.0 26.46216 7 TAAGGTA 360 0.0 26.396667 4 AACGCAG 13905 0.0 25.925898 6 AAGGTGT 1415 0.0 25.846235 6 TAGACTC 555 0.0 23.114918 5 ACGCAGA 15795 0.0 22.707054 7 >>END_MODULE