FastQCFastQC Report
Thu 26 May 2016
SRR937806_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937806_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213287
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102860.8477796267494829No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81370.6706574784037083No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51460.42413707556414926No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20310.16739650222906863No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA17390.14332964912671114No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15070.12420803981250933No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC14080.1160483875620525No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA14010.11547144245343435No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT13630.11233945472093577No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA13260.10928988771823978No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG12710.10475674757909711No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA12610.10393254028107117No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12530.10327317444265043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50850.051.8053631
GTATCAA90000.041.1576731
TATCAAC112250.032.905232
ATCAACG116500.031.7022083
TCAACGC117100.031.5397684
CAACGCA118800.031.0458645
AACGCAG121750.030.2408436
TAAGGTA3150.030.1796234
TAAGGTG6550.026.848435
ACGCAGA136400.026.7142017
CGCAGAG137000.026.5972048
GCAGAGT143000.025.0483179
GAGTACT81050.024.87997812-13
GTACATG86900.024.6234361
GTGTAAG4250.024.6144221
TACATGG86750.024.3300912
GTACTTT84300.023.75075314-15
ATGGGAG19250.023.7027635
GGTAAGG6700.023.411733
ACATGGG87500.023.3047073
Produced by FastQC (version 0.11.5)