##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937806_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1213287 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.663543745214447 33.0 31.0 34.0 30.0 34.0 2 31.76061063870296 33.0 31.0 34.0 30.0 34.0 3 31.78286505995696 33.0 31.0 34.0 30.0 34.0 4 35.40660536212784 37.0 35.0 37.0 33.0 37.0 5 35.39574807939094 37.0 35.0 37.0 33.0 37.0 6 35.49514088587449 37.0 35.0 37.0 33.0 37.0 7 35.43686118783108 37.0 35.0 37.0 33.0 37.0 8 35.41589500258389 37.0 35.0 37.0 33.0 37.0 9 36.99960355628965 39.0 37.0 39.0 33.0 39.0 10-11 36.896775865891584 39.0 37.0 39.0 33.0 39.0 12-13 36.756368855843675 39.0 37.0 39.0 32.5 39.0 14-15 37.81942689569739 40.0 37.5 41.0 32.5 41.0 16-17 37.768098149901874 40.0 37.5 41.0 32.5 41.0 18-19 37.77785717641416 40.0 37.5 41.0 33.0 41.0 20-21 37.6307435915822 40.0 37.0 41.0 32.0 41.0 22-23 37.500140527344314 40.0 37.0 41.0 32.0 41.0 24-25 37.329799956646696 39.5 37.0 41.0 32.0 41.0 26-27 37.37816691351675 40.0 37.0 41.0 32.0 41.0 28-29 37.31056543093267 40.0 37.0 41.0 32.0 41.0 30-31 37.027775785943476 39.0 36.5 41.0 31.0 41.0 32-33 36.70398677312128 39.0 35.5 41.0 30.5 41.0 34-35 36.39563351457652 39.0 35.0 40.0 30.0 41.0 36-37 36.34605991822215 39.0 35.0 41.0 30.0 41.0 38-39 36.2544369139371 39.0 35.0 41.0 30.0 41.0 40-41 35.99782203221497 39.0 35.0 41.0 29.5 41.0 42-43 35.66158913760718 39.0 35.0 40.0 27.5 41.0 44-45 35.31281304423438 38.0 35.0 40.0 26.5 41.0 46-47 34.79970938450671 38.0 34.5 40.0 24.5 41.0 48-49 34.3118841626095 38.0 34.0 40.0 22.5 41.0 50-51 33.32301096113285 37.5 33.0 39.5 20.0 40.5 52-53 33.380299962004045 37.5 33.0 39.5 20.0 40.5 54-55 33.62433702825465 38.0 33.0 40.0 18.5 41.0 56-57 33.30709304558608 37.5 33.0 40.0 16.0 41.0 58-59 33.03422726856877 37.0 33.0 40.0 13.5 41.0 60-61 32.694911014459066 36.5 32.5 40.0 10.5 41.0 62-63 32.293064625270034 36.0 32.0 39.0 8.0 41.0 64-65 31.883831690276086 35.5 31.0 39.0 6.5 40.5 66-67 31.456402318659972 35.0 31.0 39.0 2.0 40.0 68-69 30.960103833635404 35.0 30.5 38.0 2.0 40.0 70-71 30.49847933753514 35.0 30.0 37.0 2.0 40.0 72-73 30.037216256335064 35.0 29.5 37.0 2.0 39.0 74-75 29.563072463481433 34.0 29.0 36.0 2.0 39.0 76-77 29.125308356555372 34.0 29.0 36.0 2.0 38.5 78-79 28.609723420757 34.0 28.0 35.0 2.0 37.0 80-81 28.11485946853465 34.0 27.0 35.0 2.0 37.0 82-83 27.61043842058804 33.5 26.0 35.0 2.0 36.0 84-85 27.15493778471211 33.0 25.5 35.0 2.0 36.0 86-87 26.65418981658915 33.0 25.0 35.0 2.0 35.5 88-89 26.16199052656132 32.5 24.0 35.0 2.0 35.0 90-91 25.648170630691666 32.0 21.5 35.0 2.0 35.0 92-93 25.08856972834952 32.0 19.0 34.0 2.0 35.0 94-95 24.44937100620051 31.5 14.0 34.0 2.0 35.0 96-97 23.658963625259318 31.0 2.0 34.0 2.0 35.0 98-99 22.715681038369326 31.0 2.0 34.0 2.0 35.0 100-101 20.264743626198914 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32123411701715554 1101 2 0.4115437328010003 1101 3 0.312290156749615 1101 4 0.34618192111216217 1101 5 0.2533027514175501 1101 6 0.26639169329008183 1101 7 0.22302252723888927 1101 8 0.2066764085244941 1101 9 0.40586708988382014 1101 10-11 0.3090850098467115 1101 12-13 0.3371340760478603 1101 14-15 0.5759891526008474 1101 16-17 0.6561614523070745 1101 18-19 0.49648805718815936 1101 20-21 0.5170213392100678 1101 22-23 0.5675414358214823 1101 24-25 0.37697132734449923 1101 26-27 0.5124259293910569 1101 28-29 0.3593305161606324 1101 30-31 0.3103638884131783 1101 32-33 0.5277318850132389 1101 34-35 0.5676604479395664 1101 36-37 0.5236417165946605 1101 38-39 0.5885826233372953 1101 40-41 0.49686152480907 1101 42-43 0.5457895090069727 1101 44-45 0.7194028938289563 1101 46-47 0.6559808978756223 1101 48-49 0.7988783132827209 1101 50-51 0.8227456698941467 1101 52-53 0.6402188387592886 1101 54-55 0.6358917817294198 1101 56-57 0.6272291242928247 1101 58-59 0.6314597827394834 1101 60-61 0.5920579853882018 1101 62-63 0.3448246379268909 1101 64-65 0.4589857992020612 1101 66-67 0.6438405907068834 1101 68-69 0.7365432823704694 1101 70-71 0.677116327298446 1101 72-73 0.5157593718850109 1101 74-75 0.5834409435031489 1101 76-77 0.7159567655553509 1101 78-79 0.8525274760884791 1101 80-81 0.7672948167460873 1101 82-83 0.7176875441462727 1101 84-85 0.9327298055489095 1101 86-87 1.1393339299781964 1101 88-89 1.1726421331491927 1101 90-91 1.1048491089414902 1101 92-93 1.1475090435596123 1101 94-95 1.4734712647466743 1101 96-97 1.429893737423253 1101 98-99 1.819489733662941 1101 100-101 1.4421125992095014 1102 1 0.1306863594173322 1102 2 0.16949993866703394 1102 3 0.2151045484327021 1102 4 0.09488851263334652 1102 5 0.10641930327862781 1102 6 0.2869166934173606 1102 7 0.15214598270907942 1102 8 0.1181523858993927 1102 9 0.2828749149923908 1102 10-11 0.05476516298872269 1102 12-13 0.29543775339067935 1102 14-15 0.5634865433828011 1102 16-17 0.5648062371802354 1102 18-19 0.528580420961525 1102 20-21 0.43157721211441213 1102 22-23 0.5224271647988203 1102 24-25 0.4385926572565779 1102 26-27 0.29955853876184335 1102 28-29 0.38389178569158133 1102 30-31 0.4928671085700884 1102 32-33 0.4676093917717523 1102 34-35 0.46086731692881955 1102 36-37 0.3368883984329756 1102 38-39 0.7083918067665991 1102 40-41 0.7728350813387337 1102 42-43 0.7972580532924098 1102 44-45 0.7839315290424551 1102 46-47 0.8887572586146604 1102 48-49 0.9152283099734646 1102 50-51 0.8317679640713891 1102 52-53 0.8989145352041348 1102 54-55 0.8524487339590863 1102 56-57 1.0944894198854058 1102 58-59 0.9162097305551242 1102 60-61 0.8605134301262396 1102 62-63 0.8620747512051352 1102 64-65 0.9156791435322873 1102 66-67 0.9494877022388657 1102 68-69 0.8722786236561895 1102 70-71 1.265713153471065 1102 72-73 1.0191505916753556 1102 74-75 0.9860129961049822 1102 76-77 1.0851715563851592 1102 78-79 1.1770956186816122 1102 80-81 1.1362387580144997 1102 82-83 1.123993196858077 1102 84-85 1.5464502195618124 1102 86-87 1.4165049266740368 1102 88-89 1.3648768456770064 1102 90-91 1.4788742796225343 1102 92-93 1.856472025755835 1102 94-95 1.8937017414317197 1102 96-97 2.0924452217501255 1102 98-99 2.1435291595840624 1102 100-101 2.0417075976566963 1103 1 0.12075682046560843 1103 2 0.2799972346219306 1103 3 0.22244338232481198 1103 4 0.12488901460557145 1103 5 0.12104413188153273 1103 6 0.24011967249040111 1103 7 0.12827852451147947 1103 8 0.08409594836983558 1103 9 0.060974131123138875 1103 10-11 0.231588841282651 1103 12-13 0.2653077954242491 1103 14-15 0.34488028246820335 1103 16-17 0.17902261891161686 1103 18-19 0.1927890089136568 1103 20-21 0.2216558632357959 1103 22-23 0.3071917794558843 1103 24-25 0.276273862623988 1103 26-27 -0.18854841830896873 1103 28-29 0.10856821175503484 1103 30-31 0.17352347688031955 1103 32-33 0.10250940683091159 1103 34-35 0.04923450696757925 1103 36-37 0.17316011863893976 1103 38-39 0.2576963110426078 1103 40-41 0.4072035342339291 1103 42-43 0.4434479520261121 1103 44-45 0.21481074659985921 1103 46-47 0.36161350697994976 1103 48-49 0.22155738711074235 1103 50-51 0.264468812404445 1103 52-53 0.2250273944114909 1103 54-55 0.09889014719583145 1103 56-57 0.19727510152405614 1103 58-59 0.23632164394113886 1103 60-61 0.3984876287540047 1103 62-63 0.31443362435675937 1103 64-65 0.3102042117608903 1103 66-67 0.4861734174303898 1103 68-69 0.5390020924427681 1103 70-71 0.7450020437689986 1103 72-73 0.6191109622893727 1103 74-75 0.6943964606115856 1103 76-77 0.5031997566562261 1103 78-79 0.5824638153067703 1103 80-81 0.6178433254333306 1103 82-83 0.5930368340959618 1103 84-85 0.7531629091553995 1103 86-87 0.800783949254857 1103 88-89 0.9253647626772192 1103 90-91 1.1455985409968257 1103 92-93 1.257198036107031 1103 94-95 0.8775316309714078 1103 96-97 1.313218945882042 1103 98-99 1.1320313537310653 1103 100-101 1.282341720671596 1104 1 0.04756585142765957 1104 2 0.23069013241950387 1104 3 0.29314515969500476 1104 4 0.27833639949865585 1104 5 0.4107019772022866 1104 6 0.33446513153519675 1104 7 0.29318929208502453 1104 8 0.3208766665962486 1104 9 0.3345432098310823 1104 10-11 0.28604263839169164 1104 12-13 0.22929093879295692 1104 14-15 0.38177963760744404 1104 16-17 0.311753123882724 1104 18-19 0.36820605769307946 1104 20-21 0.47909250530167924 1104 22-23 0.43729935329462677 1104 24-25 0.3212912942439985 1104 26-27 0.31193468138971525 1104 28-29 0.2172911181410484 1104 30-31 0.2019299211209713 1104 32-33 0.3582811290313046 1104 34-35 0.4624103006703564 1104 36-37 0.6236164704446949 1104 38-39 0.7616975774734556 1104 40-41 0.8840866222872563 1104 42-43 0.9085328538413364 1104 44-45 0.9816209784877188 1104 46-47 0.847818326695517 1104 48-49 0.741795716295421 1104 50-51 0.5976429320894354 1104 52-53 0.7732327381674011 1104 54-55 0.7524676323844162 1104 56-57 0.7044788367672226 1104 58-59 0.747493893093214 1104 60-61 0.7948704766221795 1104 62-63 0.661393826403426 1104 64-65 0.6814622361287661 1104 66-67 0.5736317489441412 1104 68-69 0.6961932725525237 1104 70-71 0.6974517504519717 1104 72-73 0.6006039678063537 1104 74-75 0.6435145079790097 1104 76-77 0.5439866541917802 1104 78-79 0.5017252095452385 1104 80-81 0.6741312637712475 1104 82-83 0.9074000886776759 1104 84-85 0.9552286834978041 1104 86-87 0.9813631594095966 1104 88-89 0.9495888918240531 1104 90-91 0.961591233545267 1104 92-93 0.9910337567352983 1104 94-95 1.1659030300135775 1104 96-97 1.1997978721815912 1104 98-99 1.4221486441513136 1104 100-101 1.4438061951027947 1105 1 0.19273380515696203 1105 2 0.3232977319152752 1105 3 0.255391322341584 1105 4 0.21791463489870466 1105 5 0.3055004962857737 1105 6 0.26310730693587914 1105 7 0.17872678639123052 1105 8 0.2079157339790143 1105 9 0.44451688595739824 1105 10-11 0.3430487876909538 1105 12-13 0.31619944611809103 1105 14-15 0.4059094137750705 1105 16-17 0.4060536344705312 1105 18-19 0.2572286845720342 1105 20-21 0.40849341207173495 1105 22-23 0.5124627519874352 1105 24-25 0.5206635201738052 1105 26-27 0.5358577595350695 1105 28-29 0.38301566025395317 1105 30-31 0.35517843014100237 1105 32-33 0.44425479456450745 1105 34-35 0.6542283163693412 1105 36-37 0.4320348134707288 1105 38-39 0.478206142001369 1105 40-41 0.6397387075405945 1105 42-43 0.6274771711684792 1105 44-45 0.5647735669136864 1105 46-47 0.7298024952954165 1105 48-49 0.47422373612572244 1105 50-51 0.3416253846058268 1105 52-53 0.1736568597339172 1105 54-55 0.3463510393398508 1105 56-57 0.5200726864086462 1105 58-59 0.6328393873146752 1105 60-61 0.6634861427697771 1105 62-63 0.727636503917438 1105 64-65 0.6842369683879497 1105 66-67 0.6711955989033562 1105 68-69 0.6318156441375713 1105 70-71 0.737433450857683 1105 72-73 0.64543002897096 1105 74-75 0.736004921216157 1105 76-77 0.5221072514312155 1105 78-79 0.47558936352666237 1105 80-81 0.8339117480881519 1105 82-83 0.902574265858 1105 84-85 0.7902827348320045 1105 86-87 0.740139549490209 1105 88-89 0.6294929712299897 1105 90-91 0.7053492445832816 1105 92-93 0.781191567563571 1105 94-95 1.015494136040001 1105 96-97 1.1764076273748643 1105 98-99 1.309804098013032 1105 100-101 1.230237218079246 1106 1 0.1406136700338152 1106 2 0.1741933663223918 1106 3 0.18045542698175865 1106 4 0.14705172518873155 1106 5 0.2552510479165875 1106 6 0.15097734313863498 1106 7 0.17842052040027312 1106 8 0.04978602645951469 1106 9 0.17036869264511978 1106 10-11 -0.02275214363927347 1106 12-13 -0.024218371099550495 1106 14-15 0.11295613658815284 1106 16-17 0.007775835023359434 1106 18-19 -0.20863462645198894 1106 20-21 -0.18670180257496582 1106 22-23 -0.018814398316649772 1106 24-25 -0.13112824148225144 1106 26-27 0.03283487900134219 1106 28-29 0.3671348885481436 1106 30-31 0.15397751542657545 1106 32-33 0.2744253741605789 1106 34-35 0.12232772115723378 1106 36-37 0.19764417014317104 1106 38-39 0.19240787201698595 1106 40-41 0.1297294421142965 1106 42-43 0.13169167282794803 1106 44-45 0.008940572702464067 1106 46-47 0.34065951906588054 1106 48-49 0.4897035458042964 1106 50-51 0.37710189928476723 1106 52-53 0.2919550909950104 1106 54-55 0.2521457327007042 1106 56-57 0.3208276496778524 1106 58-59 0.08166480443487245 1106 60-61 -0.12408142936465794 1106 62-63 0.14060556612393071 1106 64-65 0.10909443149170173 1106 66-67 0.23981361410259794 1106 68-69 0.20924848739837998 1106 70-71 0.01816143455729602 1106 72-73 -0.0104752017705394 1106 74-75 0.14492880813289588 1106 76-77 0.4340336011489576 1106 78-79 0.2788641566387007 1106 80-81 0.3866989798237874 1106 82-83 0.36193699321649575 1106 84-85 0.2666675147499653 1106 86-87 0.23018203245548463 1106 88-89 0.4140876510729008 1106 90-91 0.8009735836471954 1106 92-93 0.7375588727185551 1106 94-95 0.41995463617588413 1106 96-97 0.35523141107644207 1106 98-99 0.6529237445187164 1106 100-101 0.48313900033609514 1107 1 0.47371737712669315 1107 2 0.597718403293932 1107 3 0.6149922417230762 1107 4 0.6153872948074692 1107 5 0.6091153662265327 1107 6 0.6442941913642528 1107 7 0.5665437028828748 1107 8 0.5016159248241934 1107 9 0.620679459202627 1107 10-11 0.6684318366745572 1107 12-13 0.6467987703279405 1107 14-15 0.863733267185502 1107 16-17 0.7375880041859233 1107 18-19 0.8444068930568136 1107 20-21 0.9366910279454501 1107 22-23 0.9029952963661358 1107 24-25 0.9839817649095792 1107 26-27 1.0143581063810103 1107 28-29 1.0475812716778066 1107 30-31 0.8712771965202819 1107 32-33 0.962401870029872 1107 34-35 0.9443905187931136 1107 36-37 0.9694222483771711 1107 38-39 1.0081443810469537 1107 40-41 0.9319484198662522 1107 42-43 0.9635397185201455 1107 44-45 0.9029068005088305 1107 46-47 0.8924556589459627 1107 48-49 0.9596066572398456 1107 50-51 0.8027107087069822 1107 52-53 0.5803425150170014 1107 54-55 0.798922654235362 1107 56-57 0.9803180679231929 1107 58-59 0.8408438563526772 1107 60-61 0.8926945668105262 1107 62-63 0.8407698174310028 1107 64-65 0.9320496012523236 1107 66-67 1.076324443378919 1107 68-69 1.0302976736818117 1107 70-71 1.118964783673146 1107 72-73 1.1345067794240968 1107 74-75 1.0751746967093325 1107 76-77 1.0701038564967646 1107 78-79 1.0636426787025322 1107 80-81 1.184928630055044 1107 82-83 1.504102625708267 1107 84-85 1.4196362907221491 1107 86-87 1.6264012535427135 1107 88-89 1.530422901956701 1107 90-91 1.7394560283783846 1107 92-93 1.873036212549973 1107 94-95 1.9072206616027039 1107 96-97 1.522416022005622 1107 98-99 1.9756955560127096 1107 100-101 1.8135561660971078 1108 1 0.36401861435100713 1108 2 0.3239224556518252 1108 3 0.2788670848286934 1108 4 0.3222906478646692 1108 5 0.36883923210424996 1108 6 0.22254593961600477 1108 7 0.2212433621979244 1108 8 0.3096793998107472 1108 9 0.43984029836347105 1108 10-11 0.5683287346483752 1108 12-13 0.3815280154417948 1108 14-15 0.43939401669241107 1108 16-17 0.3193452213277581 1108 18-19 0.404507305464918 1108 20-21 0.30216440072651807 1108 22-23 0.4138263883510689 1108 24-25 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-1.2603491131217162 2316 98-99 -1.0759265045433324 2316 100-101 -0.5413142310204115 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4412.0 3 856.0 4 839.0 5 1062.0 6 1266.0 7 1542.0 8 1858.0 9 2326.0 10 3047.0 11 4020.0 12 5810.0 13 7318.0 14 8934.0 15 11622.0 16 15187.0 17 19284.0 18 16358.0 19 10907.0 20 10212.0 21 10488.0 22 11044.0 23 12313.0 24 13643.0 25 15304.0 26 17550.0 27 20551.0 28 23971.0 29 28518.0 30 34622.0 31 42800.0 32 53191.0 33 69054.0 34 92753.0 35 128753.0 36 172735.0 37 201621.0 38 124898.0 39 12617.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.02640866898655 18.301000967078018 15.205111461940934 21.467478901994493 2 15.492132685132246 20.012823012680904 40.41380655097313 24.081237751213724 3 19.28525870872012 25.46769817481221 30.0798251150863 25.167218001381364 4 12.783009748044691 16.523340609802244 36.54012991884616 34.1535197233069 5 13.378238136472703 37.63055002875406 33.16012043653286 15.831091398240385 6 31.169883242070178 35.9002351013694 17.105090394701094 15.824791261859325 7 27.86670420981445 34.47765618458678 19.781247858265257 17.874391747333515 8 27.67549187102975 32.99216623081503 19.3220862416487 20.01025565650652 9 27.79424807627831 16.06388126850844 20.02693788431079 36.114932770902456 10-11 25.376566437513652 26.678440195935533 25.73604859491861 22.208944771632208 12-13 26.99463939694351 24.393118047638886 26.922217785905367 21.690024769512238 14-15 23.73551861305701 24.888538016814884 26.504829675189168 24.871113694938938 16-17 21.51875844535666 28.472551068475667 27.28626874286028 22.722421743307397 18-19 21.68493483244043 25.67842963687169 31.205038347459023 21.43159718322885 20-21 22.818956246400653 24.47084710287473 31.597996131799537 21.11220051892508 22-23 22.398660351005542 24.61497683647472 31.69705799902317 21.289304813496567 24-25 21.92970939459547 24.93972212726393 31.763714041771742 21.36685443636886 26-27 21.171205947766307 25.215293996704585 31.719631253682156 21.893868801846953 28-29 21.463687542966145 25.501524588505614 31.615155462609906 21.419632405918335 30-31 21.44744887826779 25.238262552678286 31.318865753404296 21.995422815649626 32-33 20.986724021251145 25.44546765691135 31.27429659361499 22.29351172822251 34-35 21.923309724764884 25.378756912669765 31.06413619807085 21.6337971644945 36-37 22.045578771630556 24.93601038365697 31.014569816386956 22.003841028325517 38-39 21.510090233565368 25.44034219322177 30.79920627156097 22.250361301651893 40-41 21.888272413441523 25.35923878651984 31.046173190018962 21.70631561001968 42-43 21.69468042680767 25.394404073547783 30.55775964780028 22.353155851844267 44-45 21.666970794632707 25.677166375061617 30.53472301923933 22.12113981106635 46-47 22.3275110053682 26.12339801348098 29.46466206511611 22.084428916034707 48-49 22.045621768568164 26.37835199763493 28.85701143806097 22.719014795735934 50-51 22.328656834734357 26.738534458555595 28.752828194414626 22.17998051229542 52-53 22.64248176201238 26.343099212918364 28.656940015220517 22.357479009848742 54-55 22.16443389410672 26.761193105583654 28.605470120755495 22.468902879554133 56-57 22.263138602368738 26.933123527381824 28.293133368227608 22.51060450202183 58-59 22.197376519448653 26.330656629681563 28.39558358345301 23.076383267416773 60-61 21.860653061595123 26.66613834643196 28.96230889150572 22.510899700467192 62-63 22.23544480507285 26.645210583735068 29.011323676857685 22.108020934334398 64-65 21.951470196707305 26.76036629537673 28.872653123561744 22.415510384354224 66-67 21.79908786247849 26.912432238765554 28.675204661320752 22.6132752374352 68-69 22.458979852141717 26.59649383511036 28.740975567471178 22.203550745276747 70-71 22.290336042704904 26.833345785627632 28.213280144837384 22.663038026830083 72-73 22.095532541081386 27.061210337525747 28.0671419077614 22.776115213631467 74-75 22.006804223556024 27.483333120039106 28.118798556259517 22.391064100145357 76-77 22.225772308992948 27.062228893082946 28.266203669303636 22.445795128620468 78-79 22.12950406519168 27.30141038104851 28.155730176127346 22.413355377632467 80-81 22.300345258597204 27.35802870715433 27.841613650511114 22.500012383737346 82-83 22.23032078995429 27.266382574780483 27.927236707698338 22.576059927566888 84-85 22.156384069799355 27.390427764559245 27.857545260003842 22.59564290563756 86-87 22.443539090607796 27.482437158230876 27.38990231011061 22.68412144105072 88-89 22.193572726083254 27.364680660847156 27.296122742201174 23.145623870868416 90-91 22.02165006965585 27.300015479077448 27.659914349104792 23.01842010216191 92-93 22.249794753871814 26.90984153636788 27.918344030072223 22.922019679688084 94-95 21.914925671924564 26.745352818440544 28.186056494703234 23.153665014931658 96-97 22.09156942006199 26.315795991201263 28.21345146434378 23.37918312439296 98-99 21.89421122418482 26.67333219947722 27.960561622052733 23.471894954285233 100-101 22.275402834192747 25.978022685594304 28.56324431817947 23.18333016203347 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1693.0 1 1048.0 2 494.5 3 610.5 4 543.5 5 506.0 6 907.5 7 2016.0 8 3335.5 9 3936.5 10 3366.5 11 2648.5 12 2569.0 13 2668.0 14 2812.5 15 2843.5 16 2843.0 17 2939.5 18 3092.5 19 3263.0 20 3498.5 21 3865.5 22 4324.5 23 4965.0 24 5804.5 25 6843.0 26 8175.0 27 9688.5 28 10983.5 29 12499.5 30 14449.5 31 16861.0 32 20551.5 33 22088.0 34 21746.0 35 22795.5 36 24760.0 37 26841.0 38 28781.0 39 30274.0 40 31453.0 41 33219.0 42 34284.5 43 34999.5 44 36407.5 45 38488.0 46 39573.5 47 41485.5 48 44148.5 49 42960.0 50 40815.0 51 39902.5 52 38973.0 53 39710.0 54 41697.0 55 40759.5 56 38188.0 57 35522.0 58 33354.0 59 30530.5 60 25269.5 61 21632.5 62 18574.5 63 13242.5 64 8770.0 65 6669.5 66 4990.0 67 3719.0 68 2888.0 69 2128.0 70 1574.5 71 1116.5 72 779.5 73 634.0 74 504.0 75 372.0 76 292.0 77 201.0 78 112.0 79 64.0 80 41.0 81 31.5 82 26.5 83 19.5 84 14.0 85 12.5 86 10.0 87 7.5 88 7.0 89 8.0 90 6.0 91 4.5 92 4.5 93 3.0 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28492846292756785 2 0.24421262240508634 3 0.23827832985929956 4 0.2466852442991642 5 0.24915786619324198 6 0.19121609314201835 7 0.18429275183860044 8 0.18528180059623156 9 0.19393597722550393 10-11 0.1960377058354701 12-13 0.19142214496652482 14-15 0.19253482481885986 16-17 0.18070745009218758 18-19 0.18585874570484973 20-21 0.17551494411462415 22-23 0.183262492716068 24-25 0.18371580672998228 26-27 0.18219102322863429 28-29 0.18993857183007812 30-31 0.18796047431481586 32-33 0.19290571810297152 34-35 0.18841378832873013 36-37 0.17745183126498512 38-39 0.16826191989199588 40-41 0.16570687726811545 42-43 0.1776990934543929 44-45 0.1791826665908396 46-47 0.1791414562259383 48-49 0.19204030044004428 50-51 0.18684779446248084 52-53 0.19958179721698163 54-55 0.17819361783320845 56-57 0.16558324617341158 58-59 0.16760255405357513 60-61 0.1566405969898301 62-63 0.16282215172502465 64-65 0.1623276273462091 66-67 0.1612561578587754 68-69 0.16418209376676746 70-71 0.16463540778068173 72-73 0.16253367917071557 74-75 0.17535010265501896 76-77 0.16888007536551533 78-79 0.1722181149225204 80-81 0.1665722949310427 82-83 0.15037662152483294 84-85 0.149552414226807 86-87 0.152601981229503 88-89 0.15680543844943529 90-91 0.16273973099522207 92-93 0.1596077432627235 94-95 0.1594429018031183 96-97 0.16290457245482726 98-99 0.17151753871919834 100-101 0.1567230177196327 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1213287.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.27586315880946 #Duplication Level Percentage of deduplicated Percentage of total 1 71.40912841965627 33.75928183457521 2 14.259799315962734 13.482886422670779 3 5.5814673299433935 7.916060571473082 4 2.8108529174031394 5.315419915307646 5 1.6220087701006896 3.834093232883452 6 1.011608630296787 2.8694802705708935 7 0.6536149539676314 2.1630147785627702 8 0.45472227029073164 1.7197910260422236 9 0.35653338289912795 1.5169881079337928 >10 1.6732953382832372 13.914303601767642 >50 0.1045574759743056 3.376469248821137 >100 0.05329495824099312 4.680422523547374 >500 0.005960616487527473 1.8420993089260878 >1k 0.0026296837444974145 1.656281498676277 >5k 3.50624499266322E-4 1.1009000774077757 >10k+ 1.75312249633161E-4 0.8525075808338765 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10286 0.8477796267494829 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8137 0.6706574784037083 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5146 0.42413707556414926 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2031 0.16739650222906863 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1739 0.14332964912671114 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1507 0.12420803981250933 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1408 0.1160483875620525 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1401 0.11547144245343435 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 1363 0.11233945472093577 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1326 0.10928988771823978 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1271 0.10475674757909711 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 1261 0.10393254028107117 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1253 0.10327317444265043 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 8.24207298025941E-5 0.0 22-23 0.0 0.0 0.0 4.945243788155647E-4 0.0 24-25 0.0 0.0 0.0 9.066280278285352E-4 0.0 26-27 0.0 0.0 0.0 0.001442362771545397 0.0 28-29 0.0 0.0 0.0 0.0031731980973998732 0.0 30-31 0.0 0.0 0.0 0.007170603492825688 0.0 32-33 0.0 0.0 0.0 0.012569161294895602 0.0 34-35 0.0 0.0 0.0 0.01846224347578108 0.0 36-37 0.0 0.0 0.0 0.02167665193808225 0.0 38-39 0.0 0.0 0.0 0.025509215873902875 0.0 40-41 0.0 0.0 0.0 0.030083566377946853 0.0 42-43 0.0 0.0 0.0 0.03577059673432584 0.0 44-45 0.0 0.0 0.0 0.04083947161718538 0.0 46-47 0.0 0.0 0.0 0.04871065131333312 0.0 48-49 0.0 0.0 0.0 0.0573648279426055 0.0 50-51 0.0 0.0 0.0 0.06923341303417906 0.0 52-53 0.0 0.0 0.0 0.09313542467693134 0.0 54-55 0.0 0.0 0.0 0.12478498492112748 0.0 56-57 0.0 0.0 0.0 0.16261609990051817 0.0 58-59 0.0 0.0 0.0 0.21363453164832394 0.0 60-61 0.0 0.0 0.0 0.28447514891365355 0.0 62-63 0.0 0.0 0.0 0.33297974840248024 0.0 64-65 0.0 0.0 0.0 0.38387454905558205 0.0 66-67 0.0 0.0 0.0 0.47816386394974975 0.0 68-69 0.0 0.0 0.0 0.5893082180885478 0.0 70-71 0.0 0.0 0.0 0.6855756304979778 0.0 72-73 0.0 0.0 0.0 0.7885603323863192 0.0 74-75 0.0 0.0 0.0 0.9551326273173618 0.0 76-77 0.0 0.0 0.0 1.0842034901882243 0.0 78-79 0.0 0.0 0.0 1.199304039357547 0.0 80-81 0.0 0.0 0.0 1.3417682708213308 0.0 82-83 0.0 0.0 0.0 1.5761728263799084 0.0 84-85 0.0 0.0 0.0 1.811236747776907 0.0 86-87 0.0 0.0 0.0 2.0890358175765504 0.0 88-89 0.0 0.0 0.0 2.4275789652407056 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5085 0.0 51.805363 1 GTATCAA 9000 0.0 41.157673 1 TATCAAC 11225 0.0 32.90523 2 ATCAACG 11650 0.0 31.702208 3 TCAACGC 11710 0.0 31.539768 4 CAACGCA 11880 0.0 31.045864 5 AACGCAG 12175 0.0 30.240843 6 TAAGGTA 315 0.0 30.179623 4 TAAGGTG 655 0.0 26.84843 5 ACGCAGA 13640 0.0 26.714201 7 CGCAGAG 13700 0.0 26.597204 8 GCAGAGT 14300 0.0 25.048317 9 GAGTACT 8105 0.0 24.879978 12-13 GTACATG 8690 0.0 24.623436 1 GTGTAAG 425 0.0 24.614422 1 TACATGG 8675 0.0 24.330091 2 GTACTTT 8430 0.0 23.750753 14-15 ATGGGAG 1925 0.0 23.702763 5 GGTAAGG 670 0.0 23.41173 3 ACATGGG 8750 0.0 23.304707 3 >>END_MODULE