FastQCFastQC Report
Thu 26 May 2016
SRR937806_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937806_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213287
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109780.9048147717728782No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96380.7943709938374021No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63130.5203220672437766No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25260.2081947634813527No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24740.20390888553161782No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA22260.18346854454057449No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA18460.15214866721558873No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA18390.15157172210697056No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15570.128329076302639No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA15330.12635097878737678No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA14510.11959247894356405No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13680.11275155836994874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56500.045.50061
GTATCAA95100.038.624891
AGGTAAG8000.032.659962
TAAGGTG9900.029.7508565
TATCAAC123800.029.5469762
ATCAACG126550.028.8298263
TCAACGC127100.028.742454
AAGGTAA8450.028.6801721
GGTAAGG9400.028.301093
CAACGCA129200.028.2385035
AACGCAG132750.027.478826
TCGCGAT350.00839547227.1414750-51
GTGTAAG4800.026.72951
GTAAGGT11150.026.4155584
TAAGGTA4300.025.4098644
ACGCAGA148650.024.4467757
CGCAGAG150500.024.0515798
GAGTACT88800.022.81556112-13
GCAGAGT158850.022.5181569
AGTACTT90600.022.30983712-13
Produced by FastQC (version 0.11.5)