##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937806_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1213287 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1755248346022 33.0 31.0 34.0 31.0 34.0 2 32.374482706894575 34.0 31.0 34.0 31.0 34.0 3 32.43993053580892 34.0 31.0 34.0 31.0 34.0 4 36.0938178683197 37.0 35.0 37.0 35.0 37.0 5 36.010712222252444 37.0 35.0 37.0 35.0 37.0 6 36.06406398486096 37.0 35.0 37.0 35.0 37.0 7 36.00694889172966 37.0 35.0 37.0 35.0 37.0 8 35.98629260842653 37.0 35.0 37.0 35.0 37.0 9 37.641909127848564 39.0 37.0 39.0 35.0 39.0 10-11 37.57794693258891 39.0 37.0 39.0 35.0 39.0 12-13 37.47772167673436 39.0 37.0 39.0 35.0 39.0 14-15 38.62922746225749 40.0 38.0 41.0 34.0 41.0 16-17 38.5989535039937 40.0 38.0 41.0 34.0 41.0 18-19 38.64531763712955 40.0 38.0 41.0 34.0 41.0 20-21 38.563227414453465 40.0 38.0 41.0 34.0 41.0 22-23 38.41416622777628 40.0 38.0 41.0 34.0 41.0 24-25 38.440213651015796 40.0 38.0 41.0 34.0 41.0 26-27 38.28784739307353 40.0 38.0 41.0 34.0 41.0 28-29 38.1268850651165 40.0 38.0 41.0 33.5 41.0 30-31 38.069740712626114 40.0 37.0 41.0 34.0 41.0 32-33 37.907338494519436 40.0 37.0 41.0 33.0 41.0 34-35 37.73515870523627 40.0 36.0 41.0 33.0 41.0 36-37 37.517029771191815 40.0 36.0 41.0 33.0 41.0 38-39 37.31794497097554 40.0 36.0 41.0 33.0 41.0 40-41 37.08074223164016 40.0 35.5 41.0 32.0 41.0 42-43 36.78340780046271 39.0 35.0 41.0 31.0 41.0 44-45 36.34445765923479 39.0 35.0 41.0 30.5 41.0 46-47 35.82114536791377 39.0 35.0 41.0 29.0 41.0 48-49 35.32751937505306 39.0 35.0 40.5 27.0 41.0 50-51 34.97711629647396 39.0 35.0 40.0 26.0 41.0 52-53 34.66432426952568 38.0 34.0 40.0 24.0 41.0 54-55 34.392666780407275 38.0 34.0 40.0 24.0 41.0 56-57 34.07881976811752 38.0 34.0 40.0 23.0 41.0 58-59 33.75815120412565 37.0 33.5 40.0 22.5 41.0 60-61 33.36519924799326 37.0 33.0 40.0 21.5 41.0 62-63 32.972121600247924 36.0 33.0 39.0 20.0 41.0 64-65 32.54999806311285 35.5 32.0 39.0 18.0 40.0 66-67 32.09830155602096 35.0 32.0 38.5 16.0 40.0 68-69 31.64321673272688 35.0 31.0 38.0 12.0 40.0 70-71 31.208631593349306 35.0 31.0 37.0 8.0 39.5 72-73 30.729767153196235 35.0 30.5 36.5 7.0 39.0 74-75 30.243535124006108 34.0 30.0 36.0 3.5 39.0 76-77 29.06930800379465 33.5 28.5 35.0 2.0 37.0 78-79 29.655838643288853 34.0 30.0 35.0 2.0 37.0 80-81 29.479274071180193 34.0 30.0 35.0 2.0 37.0 82-83 29.121797233465784 34.0 29.0 35.0 2.0 36.5 84-85 28.761586500143824 34.0 29.0 35.0 2.0 36.0 86-87 28.361649799264313 34.0 29.0 35.0 2.0 35.5 88-89 27.992137886584132 33.5 28.0 35.0 2.0 35.0 90-91 27.46328651011673 33.0 26.5 35.0 2.0 35.0 92-93 26.988469339900618 33.0 25.5 35.0 2.0 35.0 94-95 26.51529728745136 33.0 25.0 35.0 2.0 35.0 96-97 25.965075864160745 32.5 24.5 34.5 2.0 35.0 98-99 25.090417189008043 32.0 19.0 34.0 2.0 35.0 100-101 22.53580974658098 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1817510214158915 1101 2 0.22683058230501985 1101 3 0.16088428947226419 1101 4 0.14844001332686219 1101 5 0.16107871297968757 1101 6 0.18351569097274734 1101 7 0.2187069437251381 1101 8 0.15559248775437595 1101 9 0.1297710424196481 1101 10-11 0.19358521356820546 1101 12-13 0.17111760456935343 1101 14-15 0.14434605824870061 1101 16-17 0.18199315819697404 1101 18-19 0.1864349491422388 1101 20-21 0.22383098550213987 1101 22-23 0.3475496227651078 1101 24-25 0.37283861295610166 1101 26-27 0.2141202680528096 1101 28-29 0.18873867594278693 1101 30-31 0.27662315819304695 1101 32-33 0.22824244153428452 1101 34-35 0.12847175893920593 1101 36-37 0.07305527606133921 1101 38-39 0.0871025536921124 1101 40-41 7.926964348641263E-4 1101 42-43 -0.12194754951630671 1101 44-45 0.029585449707759892 1101 46-47 -0.05752613974797072 1101 48-49 -0.0953222320127125 1101 50-51 -0.22331585043043845 1101 52-53 -0.4259089079701397 1101 54-55 -0.4522248381655629 1101 56-57 -0.4049145696522345 1101 58-59 -0.394045642908857 1101 60-61 -0.36787764513586296 1101 62-63 -0.5799760370432807 1101 64-65 -0.5003978067975794 1101 66-67 -0.5526998817905522 1101 68-69 -0.6187745668219478 1101 70-71 -0.7444112184445189 1101 72-73 -0.7329991195660099 1101 74-75 -0.6671207230774101 1101 76-77 -0.7016062461474561 1101 78-79 -0.5675376024590939 1101 80-81 -0.358751386000403 1101 82-83 -0.6171762958024942 1101 84-85 -0.5808528695076269 1101 86-87 -0.6381782170782024 1101 88-89 -0.3597981989102266 1101 90-91 -0.38001120691392387 1101 92-93 -0.3652586893473284 1101 94-95 -0.19173081759624822 1101 96-97 0.001571788205581015 1101 98-99 0.08665316451235938 1101 100-101 0.49373104556356395 1102 1 0.28466316331662966 1102 2 0.2517232861676817 1102 3 0.19712212797058015 1102 4 0.08249170893675739 1102 5 0.07553658110852979 1102 6 0.11251390397658412 1102 7 0.11228546718773913 1102 8 0.12360576549227176 1102 9 0.09245947887437467 1102 10-11 0.17124877972015895 1102 12-13 0.1832625599962725 1102 14-15 0.2814473570029037 1102 16-17 0.25859267250057627 1102 18-19 0.15345926960003453 1102 20-21 0.22738766790048714 1102 22-23 0.2177986821738287 1102 24-25 0.22502501040237632 1102 26-27 0.11379290664682884 1102 28-29 0.12915457257192742 1102 30-31 0.23685562042938102 1102 32-33 0.1778875878795958 1102 34-35 0.3189266029206905 1102 36-37 0.40647643185583604 1102 38-39 0.304658390958366 1102 40-41 0.2470341496547661 1102 42-43 0.40008519680661436 1102 44-45 0.5085472609378314 1102 46-47 0.4521854971099728 1102 48-49 0.4804858619089529 1102 50-51 0.3897572229484112 1102 52-53 0.5049933978149426 1102 54-55 0.541047961309026 1102 56-57 0.24409494572051216 1102 58-59 0.30344823497464546 1102 60-61 0.3086900494354339 1102 62-63 0.3448148436759908 1102 64-65 0.3934383292177799 1102 66-67 0.44074296233349486 1102 68-69 0.4918481326114019 1102 70-71 0.33767987253954956 1102 72-73 0.32270399469483024 1102 74-75 0.36742150552572994 1102 76-77 0.3877364345504368 1102 78-79 0.5000365629729693 1102 80-81 0.396966747174087 1102 82-83 0.5882134577345859 1102 84-85 0.6500588549182176 1102 86-87 0.6632383974041041 1102 88-89 0.5759037952960355 1102 90-91 0.49120186310475944 1102 92-93 0.3259824838589296 1102 94-95 0.5621868278453839 1102 96-97 0.7130416561918729 1102 98-99 0.9181269526896791 1102 100-101 1.265933327420207 1103 1 0.21853439951568987 1103 2 0.24043895060717801 1103 3 0.20228610115997725 1103 4 0.08984692297507024 1103 5 0.07395616119556081 1103 6 0.16406856478867127 1103 7 0.15344349068532637 1103 8 0.09683849302439285 1103 9 0.26303650915386356 1103 10-11 0.1881781892158898 1103 12-13 0.13015389886187023 1103 14-15 0.29107492003343793 1103 16-17 0.3324610553838525 1103 18-19 0.19644367939959295 1103 20-21 0.29219941510727665 1103 22-23 0.408882070327806 1103 24-25 0.23836875305443073 1103 26-27 0.21167643323947516 1103 28-29 0.24897824822389936 1103 30-31 0.2178197769707424 1103 32-33 0.19108866474211084 1103 34-35 0.26883215288410156 1103 36-37 0.10717417324503486 1103 38-39 0.10762821714388338 1103 40-41 0.15288397541755927 1103 42-43 0.3733057713270256 1103 44-45 0.31662452238874295 1103 46-47 0.3188777907795739 1103 48-49 0.19335854325962742 1103 50-51 0.33488214798282456 1103 52-53 -0.028338086147044805 1103 54-55 0.023280258201822335 1103 56-57 -0.07320143188940875 1103 58-59 -0.0355690090921712 1103 60-61 -0.02382823952619617 1103 62-63 -0.02953490414901694 1103 64-65 0.017915023175881117 1103 66-67 0.19529598248899305 1103 68-69 0.21830576747681008 1103 70-71 0.18397597802768928 1103 72-73 0.1642427244937501 1103 74-75 0.1609497625989178 1103 76-77 0.09685066493455352 1103 78-79 0.2502557134774541 1103 80-81 0.1332432229063727 1103 82-83 0.26661808433330236 1103 84-85 0.3677652645751053 1103 86-87 0.5140110383328071 1103 88-89 0.4480047075645004 1103 90-91 0.6613746736472308 1103 92-93 0.3736970571693341 1103 94-95 0.36384571394260234 1103 96-97 0.3376229717289725 1103 98-99 0.4120583727162561 1103 100-101 0.7242799365978456 1104 1 0.18491029657087665 1104 2 0.22035073714807396 1104 3 0.03307565528897527 1104 4 0.01298039314116295 1104 5 0.06671421467678584 1104 6 0.05172377535099315 1104 7 0.09275670669184422 1104 8 0.12568982568812004 1104 9 0.1704524079558496 1104 10-11 0.1709530920500768 1104 12-13 0.13988776642522538 1104 14-15 0.0598275584310386 1104 16-17 0.051007716810119064 1104 18-19 0.21988083822748905 1104 20-21 0.1484922663234869 1104 22-23 0.2162135404065637 1104 24-25 -0.004496655915389169 1104 26-27 -0.004830473903496113 1104 28-29 0.1111856590278606 1104 30-31 0.11444644916895186 1104 32-33 -0.05598362371299004 1104 34-35 -0.03580178309563564 1104 36-37 0.062029370706348175 1104 38-39 -0.052647158782292536 1104 40-41 0.13248643258454962 1104 42-43 0.14802846664123592 1104 44-45 0.06602405948643764 1104 46-47 0.007517760501684734 1104 48-49 -0.01400859067634741 1104 50-51 0.135405642458565 1104 52-53 0.07885454963034988 1104 54-55 0.029287616601756383 1104 56-57 0.25103124930482323 1104 58-59 0.10200613974984662 1104 60-61 0.030873021081980312 1104 62-63 0.07879770135049569 1104 64-65 0.13806855972041632 1104 66-67 0.07049431720520971 1104 68-69 8.062769604819664E-4 1104 70-71 0.1286269453188389 1104 72-73 0.1415643324242275 1104 74-75 -0.11022747221483087 1104 76-77 -0.1076642702154551 1104 78-79 0.018963772053517403 1104 80-81 0.10758708071675827 1104 82-83 0.22693749420563591 1104 84-85 0.1610368048170372 1104 86-87 0.15187550111866344 1104 88-89 0.08860673530952567 1104 90-91 -0.26720299349980436 1104 92-93 -0.1140016414637941 1104 94-95 0.05077072522402659 1104 96-97 0.19735392795704598 1104 98-99 0.3601829269180392 1104 100-101 0.7300998777888736 1105 1 0.22666700465659773 1105 2 0.2327605633151677 1105 3 0.2092840640982061 1105 4 0.09562782272347903 1105 5 0.07826070253179296 1105 6 0.1893308981568964 1105 7 0.10660189900972483 1105 8 0.0843237384644695 1105 9 0.2092258319661866 1105 10-11 0.13978353346488603 1105 12-13 0.2553453622134114 1105 14-15 0.21191016348818437 1105 16-17 0.09527631565970296 1105 18-19 0.23473965857896673 1105 20-21 0.19783761224143603 1105 22-23 0.0879654286280811 1105 24-25 0.2719715488705745 1105 26-27 0.046422955615263106 1105 28-29 0.015651051343624545 1105 30-31 0.02387206436370093 1105 32-33 0.14034771811601843 1105 34-35 0.06808681448277554 1105 36-37 0.1088965913925719 1105 38-39 0.21222225467731448 1105 40-41 0.11459732124038169 1105 42-43 0.21716041439601952 1105 44-45 0.2544198391921597 1105 46-47 0.08182841399052165 1105 48-49 -0.13349090548233988 1105 50-51 0.1374971192437684 1105 52-53 0.00445584093023399 1105 54-55 0.017106204328577235 1105 56-57 -0.1551197713411412 1105 58-59 -0.036617921435947665 1105 60-61 -0.027373846006291558 1105 62-63 -0.12128360075435296 1105 64-65 0.031007326258908563 1105 66-67 -0.15441325792640725 1105 68-69 -0.08776998282490212 1105 70-71 -0.05917495826268748 1105 72-73 -0.11278744880690184 1105 74-75 -0.24858869190530442 1105 76-77 -0.09994388060093584 1105 78-79 0.25991791978500345 1105 80-81 0.08028864108158373 1105 82-83 0.2732604939619989 1105 84-85 0.2623770978224087 1105 86-87 0.28837696826414216 1105 88-89 0.21082538235775417 1105 90-91 0.26904927077705665 1105 92-93 0.20596252734797815 1105 94-95 0.19045211101443016 1105 96-97 0.2777483358552679 1105 98-99 0.2102129848623413 1105 100-101 0.4961795538516256 1106 1 0.08529884758931416 1106 2 0.08004648338749121 1106 3 0.12367462293859433 1106 4 0.08109110107913153 1106 5 -0.024087759168061496 1106 6 0.05320728216234727 1106 7 0.06641416851816473 1106 8 -0.0075560588226508685 1106 9 0.025814042908123724 1106 10-11 -0.020201019464209935 1106 12-13 -0.09470399237501681 1106 14-15 0.10922762543728481 1106 16-17 0.24476932223421244 1106 18-19 0.0693584876712805 1106 20-21 0.14274704903588287 1106 22-23 0.0747579717904685 1106 24-25 0.2840951057241057 1106 26-27 0.2784873855310437 1106 28-29 0.23798396740421168 1106 30-31 0.12566467089146016 1106 32-33 0.23555243756932498 1106 34-35 0.12592778709908004 1106 36-37 0.19266421224794783 1106 38-39 0.2230725194741865 1106 40-41 0.3241881639576647 1106 42-43 0.09598293079005771 1106 44-45 0.08891123346984386 1106 46-47 0.23001538973003477 1106 48-49 0.10586159892859826 1106 50-51 0.26243392988126857 1106 52-53 0.0952302644109011 1106 54-55 0.09786502584520207 1106 56-57 -0.04237481135286458 1106 58-59 0.045101941638513665 1106 60-61 0.014191377384825898 1106 62-63 0.02616728051723527 1106 64-65 0.07358463553968164 1106 66-67 0.17884567976008015 1106 68-69 -0.18119835953066143 1106 70-71 -0.03279172727617663 1106 72-73 0.13079258236886204 1106 74-75 -0.2552878204201434 1106 76-77 -0.1779047686737414 1106 78-79 -0.06649509292890698 1106 80-81 -0.10439397582402066 1106 82-83 -0.013841694678138339 1106 84-85 0.027940690611146124 1106 86-87 0.17613048257837605 1106 88-89 -0.24199275760087247 1106 90-91 -0.1812930430730617 1106 92-93 -0.12638324484363395 1106 94-95 0.3123885710301728 1106 96-97 0.1926546184620328 1106 98-99 0.33569051338732336 1106 100-101 0.4973032332115537 1107 1 0.23808255166620995 1107 2 0.32726569247931536 1107 3 0.3414931051091443 1107 4 0.1947106789438493 1107 5 0.22133385345932055 1107 6 0.26774844952046095 1107 7 0.23202266948722183 1107 8 0.25484247294031803 1107 9 0.3570653174194405 1107 10-11 0.28508321155349137 1107 12-13 0.3085582425052067 1107 14-15 0.532146466270305 1107 16-17 0.5054687911806894 1107 18-19 0.31697011772627093 1107 20-21 0.47764878100036157 1107 22-23 0.5198837264296898 1107 24-25 0.5473800119114998 1107 26-27 0.39785061825723744 1107 28-29 0.5438127039286655 1107 30-31 0.6461472458584296 1107 32-33 0.41814813944591833 1107 34-35 0.4408558918086456 1107 36-37 0.28883690831705167 1107 38-39 0.5836191327441895 1107 40-41 0.5571199940959346 1107 42-43 0.418753028655388 1107 44-45 0.47668654933444543 1107 46-47 0.5985569375137132 1107 48-49 0.4448323421464835 1107 50-51 0.4458607416580094 1107 52-53 0.42251148972394503 1107 54-55 0.4840895077724241 1107 56-57 0.619865124077613 1107 58-59 0.5490514272928735 1107 60-61 0.767469977418763 1107 62-63 0.5688439170754478 1107 64-65 0.714384934396513 1107 66-67 0.7063472777188124 1107 68-69 0.5672413815743198 1107 70-71 0.6475604062771865 1107 72-73 0.6610634042423023 1107 74-75 0.6668855919457428 1107 76-77 0.5535130705433353 1107 78-79 0.5425478111918558 1107 80-81 0.48373280527877327 1107 82-83 0.6954639087162136 1107 84-85 0.7802356677941837 1107 86-87 0.7319553634383453 1107 88-89 1.0282773274122476 1107 90-91 0.9162869705331502 1107 92-93 1.20399493586536 1107 94-95 1.131437952667273 1107 96-97 1.3365642965226208 1107 98-99 1.4921563770475998 1107 100-101 1.340367406891243 1108 1 0.12496080162138412 1108 2 0.16354146798586555 1108 3 0.1353751205296163 1108 4 0.0671277490532276 1108 5 0.03791445234761426 1108 6 0.04153363869026805 1108 7 0.06307162053617077 1108 8 0.04020557138033354 1108 9 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-0.817492715591829 2316 74-75 -0.7199412396115541 2316 76-77 -0.8328084617503357 2316 78-79 -0.7789029468125044 2316 80-81 -0.7433800778001931 2316 82-83 -0.9026423561071901 2316 84-85 -0.9247971350581246 2316 86-87 -0.8347208434559121 2316 88-89 -0.5701634000412028 2316 90-91 -0.3778693072694068 2316 92-93 -0.6424029626221781 2316 94-95 -0.8218826011984071 2316 96-97 -0.5235959349277373 2316 98-99 -0.22771647168654496 2316 100-101 0.5318797104568489 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 6.0 5 32.0 6 60.0 7 173.0 8 415.0 9 816.0 10 1437.0 11 2408.0 12 3641.0 13 5119.0 14 6656.0 15 9533.0 16 13589.0 17 19443.0 18 17309.0 19 10078.0 20 7893.0 21 7846.0 22 8014.0 23 8469.0 24 9654.0 25 11231.0 26 13280.0 27 15908.0 28 19666.0 29 24348.0 30 30141.0 31 37693.0 32 48308.0 33 62642.0 34 87412.0 35 127689.0 36 188158.0 37 247372.0 38 155859.0 39 10986.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.17990573885028 19.173990715680706 15.777159849070044 20.86894369639897 2 15.971241758957277 20.925799089580618 39.275208586261954 23.827750565200155 3 19.694532039436705 25.517066874586863 30.234915757702634 24.553485328273794 4 13.405023061377221 17.1386089324511 35.68463772702846 33.77173027914322 5 13.296912470394654 38.18856905908375 32.85420690644422 15.66031156407739 6 31.289051972039594 36.48708013849979 16.31946934237324 15.904398547087375 7 28.018020468364043 34.80998312847661 19.330133760602397 17.841862642556954 8 28.15409709326812 33.268220956789285 18.731264737856748 19.846417212085846 9 27.930077549664674 16.089927609873016 19.548960798228286 36.43103404223403 10-11 25.71567980205838 27.03148554299189 25.124517117549267 22.128317537400466 12-13 27.25657983667037 24.72870434171961 26.462144715195198 21.552571106414824 14-15 23.657180864873688 25.401656821510493 26.156630706502256 24.784531607113568 16-17 21.548075599590206 28.678787459191433 27.09931780361942 22.673819137598937 18-19 21.760515030656393 25.667298833664255 31.385978750287443 21.186207385391914 20-21 22.708312213021323 24.466099117521246 31.93176882304022 20.893819846417212 22-23 22.492038085316118 24.57491123206921 31.90605435201883 21.02699633059584 24-25 22.0388679997956 24.950753573353683 31.953513021661834 21.056865405188883 26-27 21.085708469139274 25.381702432617658 31.846319258968343 21.686269839274726 28-29 21.46276189114263 25.567754487575485 31.771008265702548 21.198475355579337 30-31 21.40524047484231 25.474104643006974 31.374316217020375 21.746338665130345 32-33 21.06987052527555 25.403758550120457 31.513895722941072 22.01247520166292 34-35 21.860285324082433 25.459722225656417 31.34052371780131 21.33946873245984 36-37 22.292870524451345 24.970596404642926 31.133730106726603 21.602802964179126 38-39 21.675213027117714 25.366762700751554 31.018157736293507 21.939866535837226 40-41 21.948781053696113 25.45419179308347 31.099831325211834 21.497195828008586 42-43 21.855865391273383 25.47274951447347 30.511715285265385 22.159669808987765 44-45 21.750830594904585 25.84227804303516 30.5013158469513 21.905575515108954 46-47 22.5805188714624 26.33544247980898 29.292492213301554 21.791546435427065 48-49 22.308283200924432 26.538856840961785 28.7411387412871 22.411721216826685 50-51 22.536300149923306 26.975769129645332 28.5155944141823 21.97233630624906 52-53 22.95800581395828 26.756488778005533 28.343870823638596 21.94163458439759 54-55 22.363422669162368 27.217385499061642 28.301630199614763 22.117561632161227 56-57 22.52533819285956 27.358901892132693 27.898922513799292 22.21683740120845 58-59 22.485364138905304 26.6983409531298 27.901972080801983 22.914322827162906 60-61 22.09984117525367 27.226163306785615 28.438201348897664 22.23579416906305 62-63 22.338943712410998 27.210544578488026 28.59991082077035 21.850600888330625 64-65 22.172082944925645 27.176463606714652 28.476444567526066 22.175008880833637 66-67 22.060650118232537 27.29741602769996 28.333691863507976 22.30824199055953 68-69 22.748878251093725 27.078573524418026 28.189113183722853 21.983435040765396 70-71 22.612919537141472 27.349434779007264 27.664282096602903 22.373363587248356 72-73 22.375497306078447 27.50173701688059 27.54092807390172 22.581837603139242 74-75 22.349946879839642 27.983692234401257 27.352926389222006 22.313434496537095 76-77 22.56082031703958 27.223649474526635 28.033680407026534 22.181849801407253 78-79 22.426186054907042 27.624626325016255 27.674696918371332 22.274490701705368 80-81 22.630475159824165 27.848904607444325 27.34189327912245 22.17872695360906 82-83 22.52173060153338 27.950548354606493 27.30184477270442 22.225876271155705 84-85 22.41972599939668 28.03008698719671 27.242924563291965 22.30726245011465 86-87 22.65572778740727 28.134192487020798 26.74144699481656 22.468632730755377 88-89 22.229200510678844 28.11482361551719 26.723602906814296 22.93237296698967 90-91 22.171629039131595 28.125569276346212 26.983966705216034 22.71883497930616 92-93 22.365985252979158 27.66558253699531 27.230062426747065 22.73836978327847 94-95 21.966855327717187 27.47095287429932 27.53219147654265 23.030000321440845 96-97 22.33024832541682 27.062764209951972 27.612469267370376 22.99451819726083 98-99 21.92632197981398 27.401009919098502 27.178799693883988 23.493868407203536 100-101 21.67963372391132 27.51303712716619 27.766978766340717 23.040350382581774 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 906.0 1 628.5 2 461.5 3 610.5 4 552.5 5 545.5 6 992.0 7 2128.5 8 3394.0 9 3956.0 10 3526.5 11 2886.5 12 2748.0 13 2838.0 14 2970.0 15 3077.5 16 3090.0 17 3128.5 18 3401.5 19 3638.5 20 3790.0 21 4084.0 22 4604.5 23 5195.0 24 5875.0 25 6839.0 26 8060.0 27 9552.5 28 10893.0 29 12550.5 30 14611.5 31 16753.5 32 20191.5 33 21666.5 34 21242.0 35 22254.5 36 24450.0 37 26680.0 38 28666.0 39 30232.5 40 31264.5 41 32676.0 42 33426.0 43 34728.5 44 36140.0 45 37628.5 46 38604.0 47 40201.0 48 43510.5 49 42805.5 50 40940.5 51 40231.5 52 38877.5 53 39873.0 54 42847.0 55 42165.5 56 38884.0 57 36501.5 58 34740.0 59 31289.0 60 25353.0 61 21534.0 62 18695.5 63 13296.5 64 8491.5 65 6306.5 66 4784.0 67 3689.5 68 2852.0 69 2200.0 70 1629.0 71 1030.0 72 738.5 73 615.5 74 467.5 75 346.5 76 237.0 77 156.5 78 106.0 79 65.5 80 46.0 81 31.5 82 19.0 83 16.5 84 14.0 85 5.5 86 1.0 87 0.5 88 1.0 89 2.0 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022583279965910787 2 0.0 3 8.242072980259412E-5 4 2.472621894077823E-4 5 0.02027549953143815 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.002884725543090794 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.066280278285352E-4 24-25 8.242072980259412E-5 26-27 4.533140139142676E-4 28-29 0.0016896249609531793 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 2.0605182450648526E-4 40-41 4.533140139142676E-4 42-43 0.014093944796243593 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.472621894077823E-4 70-71 4.121036490129706E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.121036490129706E-5 82-83 0.0017308353258544762 84-85 4.121036490129705E-4 86-87 0.0 88-89 0.0 90-91 0.01022017049552167 92-93 0.0020605182450648524 94-95 0.0 96-97 0.0 98-99 0.0017720456907557734 100-101 0.017555615447952543 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1213287.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.696480922376466 #Duplication Level Percentage of deduplicated Percentage of total 1 69.14260064631364 30.90430930711452 2 15.223896310353005 13.609091819998612 3 6.048362038447072 8.110214953892267 4 3.044567139208521 5.443257482181118 5 1.7514859250183246 3.9142628616696222 6 1.1054729871315256 2.964645136771605 7 0.7315489287280345 2.2888363915674272 8 0.5165961964736767 1.8472025632206346 9 0.37734725189893253 1.5179484821050642 >10 1.8751388960498856 14.773163352540111 >50 0.11522133898131878 3.5638180218014264 >100 0.05739433659193028 4.781350943956875 >500 0.006850288888444808 2.0274333140732295 >1k 0.002962287086895052 2.0260265236104034 >5k 3.702858858618815E-4 1.319983216031922 >10k+ 1.8514294293094075E-4 0.9084556294651394 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10978 0.9048147717728782 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9638 0.7943709938374021 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6313 0.5203220672437766 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2526 0.2081947634813527 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2474 0.20390888553161782 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2226 0.18346854454057449 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1846 0.15214866721558873 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1839 0.15157172210697056 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1557 0.128329076302639 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1533 0.12635097878737678 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 1451 0.11959247894356405 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1368 0.11275155836994874 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 8.24207298025941E-5 0.0 22-23 0.0 0.0 0.0 4.945243788155647E-4 0.0 24-25 0.0 0.0 0.0 9.066280278285352E-4 0.0 26-27 0.0 0.0 0.0 0.001442362771545397 0.0 28-29 0.0 0.0 0.0 0.003255618827202467 0.0 30-31 0.0 0.0 0.0 0.0073354449524308755 0.0 32-33 0.0 0.0 0.0 0.0126103716597969 0.0 34-35 0.0 0.0 0.0 0.018585874570484974 0.0 36-37 0.0 0.0 0.0 0.021759072667884842 0.0 38-39 0.0 0.0 0.0 0.02546800550900158 0.0 40-41 0.0 0.0 0.0 0.02991872491834166 0.0 42-43 0.0 0.0 0.0 0.03585301746412843 0.0 44-45 0.0 0.0 0.0 0.04121036490129705 0.0 46-47 0.0 0.0 0.0 0.048957913502740896 0.0 48-49 0.0 0.0 0.0 0.0574060383075068 0.0 50-51 0.0 0.0 0.0 0.06906857157457386 0.0 52-53 0.0 0.0 0.0 0.09313542467693134 0.0 54-55 0.0 0.0 0.0 0.1247849849211275 0.0 56-57 0.0 0.0 0.0 0.16236883771111038 0.0 58-59 0.0 0.0 0.0 0.2136345316483239 0.0 60-61 0.0 0.0 0.0 0.28517572511697564 0.0 62-63 0.0 0.0 0.0 0.33442211117402565 0.0 64-65 0.0 0.0 0.0 0.38527570146222617 0.0 66-67 0.0 0.0 0.0 0.47968864745109774 0.0 68-69 0.0 0.0 0.0 0.5915335777932179 0.0 70-71 0.0 0.0 0.0 0.6893669840688972 0.0 72-73 0.0 0.0 0.0 0.7939176798234877 0.0 74-75 0.0 0.0 0.0 0.9620147582558785 0.0 76-77 0.0 0.0 0.0 1.0932697704665095 0.0 78-79 0.0 0.0 0.0 1.2103484171510945 0.0 80-81 0.0 0.0 0.0 1.3550792186844498 0.0 82-83 0.0 0.0 0.0 1.5929866552596375 0.0 84-85 0.0 0.0 0.0 1.8321304027818646 0.0 86-87 0.0 0.0 0.0 2.1152456096537753 0.0 88-89 0.0 0.0 0.0 2.459846680958421 8.24207298025941E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5650 0.0 45.5006 1 GTATCAA 9510 0.0 38.62489 1 AGGTAAG 800 0.0 32.65996 2 TAAGGTG 990 0.0 29.750856 5 TATCAAC 12380 0.0 29.546976 2 ATCAACG 12655 0.0 28.829826 3 TCAACGC 12710 0.0 28.74245 4 AAGGTAA 845 0.0 28.680172 1 GGTAAGG 940 0.0 28.30109 3 CAACGCA 12920 0.0 28.238503 5 AACGCAG 13275 0.0 27.47882 6 TCGCGAT 35 0.008395472 27.14147 50-51 GTGTAAG 480 0.0 26.7295 1 GTAAGGT 1115 0.0 26.415558 4 TAAGGTA 430 0.0 25.409864 4 ACGCAGA 14865 0.0 24.446775 7 CGCAGAG 15050 0.0 24.051579 8 GAGTACT 8880 0.0 22.815561 12-13 GCAGAGT 15885 0.0 22.518156 9 AGTACTT 9060 0.0 22.309837 12-13 >>END_MODULE