FastQCFastQC Report
Thu 26 May 2016
SRR937805_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937805_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134808
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100980.8898421583210551No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78610.6927163009072901No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50020.44077940938026516No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19800.17447885457275591No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA15980.1408167725289212No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14850.13085914092956694No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT13970.1231045251707778No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC13580.11966782045949624No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA13040.11490930624387562No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12480.10997455076100979No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG12000.1057447603471248No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA11880.10468731274365356No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA11450.10089812549788157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49350.046.905171
GTATCAA83900.039.769861
TATCAAC107450.030.9635942
ATCAACG110400.030.0487843
TCAACGC110650.029.976924
CAACGCA112200.029.56285
AACGCAG114950.028.8854126
TAAGGTG6450.028.733465
AGGTAAG6100.027.2708032
GGTAAGG6650.026.4436023
ACGCAGA128900.025.7224837
CGCAGAG129150.025.415238
GAGTACT75500.024.88121412-13
GTACATG79300.024.1552071
TACATGG79700.023.854012
GTATAGT2002.0396328E-823.7656081
GTACTTT78100.023.6859114-15
GCAGAGT137000.023.5775539
GTAAGGT7500.023.4435544
AGTACTT76350.023.20447712-13
Produced by FastQC (version 0.11.5)