##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937805_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1134808 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76599653862151 33.0 31.0 34.0 30.0 34.0 2 31.875778986401222 33.0 31.0 34.0 30.0 34.0 3 31.8685222522224 33.0 31.0 34.0 30.0 34.0 4 35.50219949101522 37.0 35.0 37.0 33.0 37.0 5 35.49658356303445 37.0 35.0 37.0 33.0 37.0 6 35.56806702102911 37.0 35.0 37.0 33.0 37.0 7 35.52072068579002 37.0 35.0 37.0 33.0 37.0 8 35.49073235296191 37.0 35.0 37.0 33.0 37.0 9 37.10823064342162 39.0 37.0 39.0 33.0 39.0 10-11 37.000202236854165 39.0 37.0 39.0 33.0 39.0 12-13 36.86372716794383 39.0 37.0 39.0 33.0 39.0 14-15 37.955299486785435 40.0 38.0 41.0 33.0 41.0 16-17 37.89358860705952 40.0 38.0 41.0 33.0 41.0 18-19 37.89540080788997 40.0 38.0 41.0 33.0 41.0 20-21 37.840058406355965 40.0 38.0 41.0 33.0 41.0 22-23 37.650927293427614 40.0 37.0 41.0 32.0 41.0 24-25 37.46218479249353 40.0 37.0 41.0 32.0 41.0 26-27 37.37660643915094 39.5 37.0 41.0 32.0 41.0 28-29 37.39040304615406 40.0 37.0 41.0 32.0 41.0 30-31 37.129476968791195 39.0 36.5 41.0 31.5 41.0 32-33 36.803119558550875 39.0 35.5 41.0 31.0 41.0 34-35 36.77606916764775 39.0 35.0 41.0 31.0 41.0 36-37 36.64527391417755 39.0 35.0 41.0 30.5 41.0 38-39 36.46582197164631 39.0 35.0 41.0 30.0 41.0 40-41 36.16110654842053 39.0 35.0 41.0 30.0 41.0 42-43 35.82846040916173 39.0 35.0 40.5 29.0 41.0 44-45 35.47350961572354 39.0 35.0 40.0 27.5 41.0 46-47 34.96046423712205 38.0 35.0 40.0 25.0 41.0 48-49 34.44672182430861 38.0 34.0 40.0 23.0 41.0 50-51 33.416454589675084 37.5 33.0 39.5 20.5 40.5 52-53 33.45801448350734 37.5 33.0 39.5 20.0 40.5 54-55 33.755702726804884 38.0 34.0 40.0 19.5 41.0 56-57 33.49829574694574 38.0 33.0 40.0 18.0 41.0 58-59 33.21625332214789 37.0 33.0 40.0 15.0 41.0 60-61 32.86134835143919 37.0 33.0 40.0 12.0 41.0 62-63 32.462468100330625 36.0 32.0 39.5 8.0 41.0 64-65 32.05270847579503 35.5 32.0 39.0 7.0 41.0 66-67 31.623833723414005 35.0 31.0 39.0 3.5 40.0 68-69 31.125642399419107 35.0 31.0 38.0 2.0 40.0 70-71 30.66773189825944 35.0 30.0 37.5 2.0 40.0 72-73 30.230754013013655 35.0 30.0 37.0 2.0 39.0 74-75 29.792964096129037 34.5 29.0 36.0 2.0 39.0 76-77 29.324873018166947 34.0 29.0 36.0 2.0 38.5 78-79 28.81881648701807 34.0 28.5 35.0 2.0 37.5 80-81 28.323293015206097 34.0 27.5 35.0 2.0 37.0 82-83 27.81417869807051 34.0 26.5 35.0 2.0 36.5 84-85 27.37661304819846 33.0 26.0 35.0 2.0 36.0 86-87 26.927969312870545 33.0 25.0 35.0 2.0 35.5 88-89 26.431639537260928 33.0 24.5 35.0 2.0 35.0 90-91 25.93377117538826 32.5 23.5 35.0 2.0 35.0 92-93 25.370994035995516 32.0 20.0 34.5 2.0 35.0 94-95 24.73103776145392 32.0 17.5 34.0 2.0 35.0 96-97 24.01921646657408 31.0 4.5 34.0 2.0 35.0 98-99 23.083300875566614 31.0 2.0 34.0 2.0 35.0 100-101 20.77458257255853 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.017979010099729464 1101 2 0.09008854911143871 1101 3 0.02158203629165456 1101 4 -0.10261641510356867 1101 5 -0.04641414090549745 1101 6 -0.06978177743570058 1101 7 -0.11446228727464813 1101 8 -0.07429301923186671 1101 9 0.018947738326332342 1101 10-11 -1.3183845787523296E-5 1101 12-13 0.11291248105375473 1101 14-15 0.3278141792071523 1101 16-17 0.29292165340094556 1101 18-19 0.3558137498212588 1101 20-21 0.3302833851080891 1101 22-23 0.24959088598227908 1101 24-25 0.2890473394392572 1101 26-27 0.33832851229199434 1101 28-29 0.1487121214906324 1101 30-31 0.4395714369279631 1101 32-33 0.3699870857954579 1101 34-35 0.5071416765867198 1101 36-37 0.4385429315203524 1101 38-39 0.5397478802442635 1101 40-41 0.5144270661725727 1101 42-43 0.5671792722924138 1101 44-45 0.6058405056883274 1101 46-47 0.7557188798260981 1101 48-49 0.7431559922727047 1101 50-51 0.8577394204840445 1101 52-53 0.8179410362581123 1101 54-55 1.0350374173996215 1101 56-57 0.9100602181012292 1101 58-59 0.8962967285273891 1101 60-61 0.924923109978856 1101 62-63 0.9630610238227106 1101 64-65 0.7211274632715394 1101 66-67 0.8389563759067116 1101 68-69 0.8051267817386432 1101 70-71 0.9446734770499532 1101 72-73 0.992496036336945 1101 74-75 1.1649425023496 1101 76-77 1.199909413173014 1101 78-79 1.171327853537079 1101 80-81 1.0814638163140877 1101 82-83 1.0750264730727928 1101 84-85 1.2164424811779107 1101 86-87 1.2317141212347629 1101 88-89 1.339994064125829 1101 90-91 1.5195048956860546 1101 92-93 1.456044659486512 1101 94-95 1.7911283262165654 1101 96-97 1.7525916288309418 1101 98-99 2.0794141458693858 1101 100-101 2.273796429384113 1102 1 0.07093712971835586 1102 2 0.1866823602415444 1102 3 0.1998615366228158 1102 4 0.09600780127583874 1102 5 0.01645356391405528 1102 6 0.1177141793130616 1102 7 0.08259576611166608 1102 8 0.15962161810775655 1102 9 0.12625208347366623 1102 10-11 0.09807901768525795 1102 12-13 0.2302561034988031 1102 14-15 0.3137251814713764 1102 16-17 0.34722444285922904 1102 18-19 0.373162609239337 1102 20-21 0.3183178259071937 1102 22-23 0.3799958272203625 1102 24-25 0.5099120561036159 1102 26-27 0.3223828535886071 1102 28-29 0.18164960127437269 1102 30-31 0.30425698750325836 1102 32-33 0.30860298462945934 1102 34-35 0.298944889623705 1102 36-37 0.5416438356683457 1102 38-39 0.5386595140258237 1102 40-41 0.4722839119745288 1102 42-43 0.5089881843882864 1102 44-45 0.6199396693129344 1102 46-47 0.6970915828743145 1102 48-49 0.5402531689281247 1102 50-51 0.6005846371252943 1102 52-53 0.6094050782771205 1102 54-55 0.6825749618753889 1102 56-57 0.6044754484510264 1102 58-59 0.7260612187696793 1102 60-61 0.7540348269358574 1102 62-63 0.8660303875304862 1102 64-65 0.8549247308038481 1102 66-67 0.8465656977187024 1102 68-69 0.8700285884943391 1102 70-71 0.8014746107005841 1102 72-73 0.8660151281842126 1102 74-75 1.0400625676577313 1102 76-77 0.8639443468636934 1102 78-79 0.917761936989784 1102 80-81 0.9590082920776872 1102 82-83 1.139302923637306 1102 84-85 0.9757025276945548 1102 86-87 1.083379632686789 1102 88-89 1.115028181464826 1102 90-91 1.0695774431653753 1102 92-93 1.1420357566323638 1102 94-95 1.2583319366473837 1102 96-97 1.28685129027604 1102 98-99 1.7515211033420854 1102 100-101 2.3013453719776464 1103 1 0.21043201636300424 1103 2 0.27116552047064957 1103 3 0.2919319270247094 1103 4 0.25587889047636736 1103 5 0.24126761646753891 1103 6 0.24896388016981064 1103 7 0.29442428922148167 1103 8 0.4047097656531804 1103 9 0.3917004792681098 1103 10-11 0.24328522710148803 1103 12-13 0.28826755869113185 1103 14-15 0.43375102744850125 1103 16-17 0.26469281344586193 1103 18-19 0.12665548564132934 1103 20-21 0.26570562724415936 1103 22-23 0.33025923282670533 1103 24-25 0.48574766292567517 1103 26-27 0.32878882688110167 1103 28-29 0.257014556043913 1103 30-31 0.1621672923900661 1103 32-33 0.38733780809505447 1103 34-35 0.43669405601117006 1103 36-37 0.41252148208899797 1103 38-39 0.5190226340500672 1103 40-41 0.4827879085283939 1103 42-43 0.4628342891442614 1103 44-45 0.5411687891138186 1103 46-47 0.4811018574479391 1103 48-49 0.4749901621794521 1103 50-51 0.35952271846034733 1103 52-53 0.36914821393659025 1103 54-55 0.40287458375831875 1103 56-57 0.46632774347806816 1103 58-59 0.391094164413353 1103 60-61 0.5277842485922335 1103 62-63 0.5108310384662076 1103 64-65 0.5958114711794131 1103 66-67 0.4374580221016693 1103 68-69 0.4105005168914104 1103 70-71 0.5465678636242117 1103 72-73 0.5806078945014796 1103 74-75 0.6847815325423063 1103 76-77 0.5533025050985039 1103 78-79 0.7445776168825695 1103 80-81 0.8535366029952165 1103 82-83 0.895649387365193 1103 84-85 0.6926472779298791 1103 86-87 0.8381277939529248 1103 88-89 0.6567499702617177 1103 90-91 0.9141062327121148 1103 92-93 1.0662559898843291 1103 94-95 0.9973437268801568 1103 96-97 1.1216042378342337 1103 98-99 1.021562105910732 1103 100-101 1.4904377314600872 1104 1 0.3082884636945664 1104 2 0.277659537997895 1104 3 0.3214848088825413 1104 4 0.2014690677496418 1104 5 0.17600997664307982 1104 6 0.18430048596687243 1104 7 0.12316150060218689 1104 8 0.1413702519875315 1104 9 0.34677924515801095 1104 10-11 0.4718800462934283 1104 12-13 0.2948816258388831 1104 14-15 0.5264059915003045 1104 16-17 0.681851572399566 1104 18-19 0.49767884196193535 1104 20-21 0.5026492306002197 1104 22-23 0.581511417062849 1104 24-25 0.4448124096876711 1104 26-27 0.4151842036368194 1104 28-29 0.4074210573978121 1104 30-31 0.6819377975321927 1104 32-33 0.7695898055873513 1104 34-35 0.6911162171711211 1104 36-37 0.7854512930775357 1104 38-39 0.8914062192652992 1104 40-41 0.9813629862271824 1104 42-43 0.9449508973423164 1104 44-45 0.9436474803061756 1104 46-47 1.2074564173326863 1104 48-49 1.0999831592849176 1104 50-51 0.9625486358395321 1104 52-53 1.0512678233751558 1104 54-55 1.1455498895513756 1104 56-57 1.171490877573497 1104 58-59 1.1191100776574956 1104 60-61 1.2238066044993445 1104 62-63 1.338407436710817 1104 64-65 1.245983875773316 1104 66-67 1.0964564081012185 1104 68-69 1.1525695721008944 1104 70-71 1.0271174908034482 1104 72-73 1.3002613512636039 1104 74-75 1.499066103498297 1104 76-77 1.560704231145273 1104 78-79 1.5218265464401206 1104 80-81 1.308280230361195 1104 82-83 1.5832309210657876 1104 84-85 1.639804102750805 1104 86-87 1.9221661964926398 1104 88-89 1.7127684579032731 1104 90-91 1.3776391596552493 1104 92-93 1.6380740079984832 1104 94-95 1.7412168611089704 1104 96-97 1.8886176033586466 1104 98-99 2.21768150192775 1104 100-101 2.438706349155403 1105 1 0.3224865606795504 1105 2 0.10920258511471204 1105 3 0.32860965125328434 1105 4 0.19951733096183943 1105 5 0.16985956967300098 1105 6 0.21413540514605245 1105 7 0.31074604720312493 1105 8 0.16516940365553978 1105 9 0.3301880494684539 1105 10-11 0.3685555019852771 1105 12-13 0.4754158457173645 1105 14-15 0.5611130876828057 1105 16-17 0.5560548281601996 1105 18-19 0.5289109295464911 1105 20-21 0.6226939399891904 1105 22-23 0.6559052609239089 1105 24-25 0.628765751427899 1105 26-27 0.6109081518197925 1105 28-29 0.516984854256691 1105 30-31 0.6066798587496649 1105 32-33 0.6611365784865839 1105 34-35 0.6186655121384419 1105 36-37 0.6889707244658254 1105 38-39 0.7257221082942991 1105 40-41 0.9523620691115013 1105 42-43 0.9087348592820206 1105 44-45 0.8632880856025196 1105 46-47 0.6876352548885762 1105 48-49 0.7238998163983013 1105 50-51 0.6973517425688698 1105 52-53 0.6275790121863452 1105 54-55 0.8494003423772369 1105 56-57 0.654402433230949 1105 58-59 0.6788515656776681 1105 60-61 0.8656356770661233 1105 62-63 0.8516597581598617 1105 64-65 0.8140883123203757 1105 66-67 0.8301962338492359 1105 68-69 0.9125460710118176 1105 70-71 0.8909731024049563 1105 72-73 0.9159870190384538 1105 74-75 0.9796834369844838 1105 76-77 1.023927892729592 1105 78-79 1.0099441665249564 1105 80-81 1.0370461365861168 1105 82-83 1.0298476240349501 1105 84-85 1.0821347229502756 1105 86-87 1.3301909586157628 1105 88-89 1.2966214901169586 1105 90-91 1.3284287389284373 1105 92-93 1.52449496409157 1105 94-95 1.43675165418475 1105 96-97 1.4974090168631768 1105 98-99 1.8172673668824686 1105 100-101 2.303030549545177 1106 1 0.2213545243990005 1106 2 0.25082136237705654 1106 3 0.2688229033477221 1106 4 0.25299479409276415 1106 5 0.09115362215517564 1106 6 0.24601936277258574 1106 7 0.23479922175135215 1106 8 -0.011137457799840433 1106 9 0.30368559153035335 1106 10-11 0.20703261892773384 1106 12-13 0.24273911650598734 1106 14-15 0.29261277611319514 1106 16-17 0.13174201226323135 1106 18-19 0.3216522229065859 1106 20-21 0.41956612720790787 1106 22-23 0.4461072618932391 1106 24-25 0.4848586684119027 1106 26-27 0.3497037621500496 1106 28-29 0.42655350342930376 1106 30-31 0.2998772554124045 1106 32-33 0.43910774098744554 1106 34-35 0.3079276114252991 1106 36-37 0.4614089298248203 1106 38-39 0.5167064951945264 1106 40-41 0.8090573540246169 1106 42-43 0.7753112284574044 1106 44-45 0.6737602866687809 1106 46-47 0.8144804394070277 1106 48-49 0.9648833378273665 1106 50-51 0.8294858213613665 1106 52-53 0.7336935659494159 1106 54-55 0.800618060302881 1106 56-57 0.9463042195634301 1106 58-59 0.9033374758071417 1106 60-61 0.9569099968057841 1106 62-63 1.0753373182235535 1106 64-65 1.0302518517512418 1106 66-67 1.0824732884777788 1106 68-69 0.7171573013656243 1106 70-71 0.9697526149716005 1106 72-73 0.9396299602556546 1106 74-75 1.2024989877703298 1106 76-77 1.4236647894961898 1106 78-79 1.615135262557633 1106 80-81 1.3452750363991761 1106 82-83 0.9631448960698457 1106 84-85 1.1061082741515484 1106 86-87 1.3174875728925741 1106 88-89 1.3895904023928018 1106 90-91 1.4645162899912343 1106 92-93 1.5362601790746062 1106 94-95 1.9690735402195045 1106 96-97 1.9490663941393649 1106 98-99 2.1213619109522597 1106 100-101 2.3926829763263164 1107 1 0.3105856926046897 1107 2 0.33702480966284654 1107 3 0.32162972752874097 1107 4 0.4090025370560255 1107 5 0.4531695711775754 1107 6 0.33166894737557584 1107 7 0.4548549784963427 1107 8 0.26284581517666794 1107 9 0.29871379406996823 1107 10-11 0.47483170940741104 1107 12-13 0.3467402649837865 1107 14-15 0.5118872900940588 1107 16-17 0.6703044700057248 1107 18-19 0.6671148093897514 1107 20-21 0.7457379913541047 1107 22-23 0.6530870431267388 1107 24-25 0.6468991615164015 1107 26-27 0.6982283386488604 1107 28-29 0.600050525722736 1107 30-31 0.6450383664720576 1107 32-33 0.6529640330712851 1107 34-35 0.7330463955207378 1107 36-37 0.5842187178195175 1107 38-39 0.8358582887649035 1107 40-41 0.7035918963469499 1107 42-43 0.5449631655874967 1107 44-45 0.740923546493363 1107 46-47 0.7112660908440134 1107 48-49 0.6762824431261123 1107 50-51 0.45220113779478055 1107 52-53 0.5982042125238962 1107 54-55 0.6741789898289454 1107 56-57 0.666654505293593 1107 58-59 0.8332733833361559 1107 60-61 0.9946342509898614 1107 62-63 1.0166945596539705 1107 64-65 0.966371015585306 1107 66-67 0.8486922957854333 1107 68-69 0.9304963153418555 1107 70-71 1.0854710156236393 1107 72-73 1.141553197481155 1107 74-75 0.9742367087828434 1107 76-77 1.1758279103920053 1107 78-79 1.4783881406985806 1107 80-81 1.3684992952142032 1107 82-83 1.2240887781100618 1107 84-85 1.4626828464824975 1107 86-87 1.4378883360745967 1107 88-89 1.6906143074025728 1107 90-91 1.8011374497685786 1107 92-93 1.7711595483380087 1107 94-95 1.8385281985010486 1107 96-97 2.1356889432043786 1107 98-99 2.5345031829840465 1107 100-101 3.182832926775056 1108 1 0.15900840385538473 1108 2 0.07168496965032745 1108 3 0.13674068371891934 1108 4 0.22564472068238217 1108 5 0.22566403137100366 1108 6 0.2507157449583346 1108 7 0.19065511152283676 1108 8 0.0786076764093977 1108 9 0.22109514617402937 1108 10-11 0.3617120602559041 1108 12-13 0.4365820559330018 1108 14-15 0.46632527093682086 1108 16-17 0.6058168532074006 1108 18-19 0.5597230356912917 1108 20-21 0.7122203831278 1108 22-23 0.6593568948413306 1108 24-25 0.5826996502287187 1108 26-27 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-0.500531835613792 2316 100-101 -0.3783619537612566 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4165.0 3 840.0 4 746.0 5 911.0 6 1019.0 7 1328.0 8 1498.0 9 2001.0 10 2610.0 11 3606.0 12 5122.0 13 6807.0 14 7825.0 15 10273.0 16 13965.0 17 18365.0 18 15585.0 19 10039.0 20 9184.0 21 9403.0 22 9975.0 23 10891.0 24 12055.0 25 13488.0 26 15408.0 27 18244.0 28 21443.0 29 25357.0 30 31041.0 31 38265.0 32 47709.0 33 62288.0 34 84393.0 35 119050.0 36 163738.0 37 196330.0 38 126730.0 39 13110.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.06888425271786 18.211757553586214 15.278333735390095 21.44102445830583 2 15.558025419970209 19.935487062956216 40.533950131906074 23.972537385167502 3 19.211609863224744 25.475550859847544 30.16864796294709 25.144191313980617 4 12.732659204612906 16.567649594392154 36.62571723338704 34.0739739676079 5 13.264956419433362 37.634301280035615 33.2770395599193 15.823702740611717 6 31.104626265391083 35.90713033804098 17.154751866355276 15.83349153021266 7 27.796627228495076 34.56071068167913 19.793777297492007 17.84888479233379 8 27.6249299138658 32.91756987642766 19.383771803961906 20.073728405744635 9 27.684989699861106 16.07545723698443 20.052820772285255 36.18673229086921 10-11 25.31505240571827 26.668903585840305 25.800611032132164 22.21543297630926 12-13 26.934441753841465 24.412505490963916 26.942918256930753 21.710134498263862 14-15 23.646924511375918 24.87144259770716 26.61787195117644 24.863760939740484 16-17 21.413258742641805 28.43302156755234 27.3999828709823 22.753736818823555 18-19 21.588217609219374 25.68380892508773 31.38168318733078 21.34629027836212 20-21 22.68115503814034 24.440541367296284 31.767389157734293 21.110914436829077 22-23 22.30705094563895 24.555671737116388 31.879523890023485 21.257753427221175 24-25 21.884648471759316 24.812906546185324 32.00294494255111 21.299500039504252 26-27 21.043953788243645 25.148379185021398 31.947710490965697 21.85995653576926 28-29 21.3757208028754 25.391554735544098 31.8109552765574 21.421769185023106 30-31 21.313394012464578 25.216951365898517 31.495462309377043 21.974192312259866 32-33 20.90442473031405 25.360633489356932 31.4910716887369 22.24387009159212 34-35 21.837946845209032 25.281900865193506 31.29737528243063 21.58277700716683 36-37 21.935410682843347 24.921043327184535 31.203588815276916 21.9399571746952 38-39 21.46771528696468 25.316526098753762 31.007396754644656 22.208361859636902 40-41 21.776742190575902 25.34697271711285 31.173563159539476 21.702721932771773 42-43 21.629301523593764 25.301791990648486 30.712196431204315 22.35671005455343 44-45 21.5439888866278 25.630413277425102 30.71565508464588 22.10994275130122 46-47 22.263480973153264 26.018483449561174 29.698928832186063 22.019106745099492 48-49 21.941618219727854 26.329412107489297 29.036907671884908 22.692062000897938 50-51 22.211233827928808 26.73870736453959 28.874762915582053 22.17529589194955 52-53 22.51496937244982 26.391176934207174 28.779035239896306 22.314818453446698 54-55 22.06161373855878 26.761883992841483 28.791881247036784 22.384621021562953 56-57 22.13702085817658 26.85970181925385 28.46508676122326 22.538190561346305 58-59 22.133645800233026 26.22431416163542 28.61239098965505 23.029649048476504 60-61 21.810079132591337 26.52020950782785 29.08993326980599 22.57977808977482 62-63 22.10457457486619 26.635848162145415 29.199804724915495 22.059772538072902 64-65 21.86019681600656 26.76382564698727 28.94480510166297 22.431172435343203 66-67 21.76600429809102 26.834512020133673 28.82099982477489 22.57848385700042 68-69 22.300195843803422 26.59075089347942 28.896252219629314 22.212801043087843 70-71 22.193548330146694 26.80115884306363 28.394744600804696 22.61054822598499 72-73 22.06419987614995 27.021159114653102 28.206120734820097 22.708520274376852 74-75 21.97830087203464 27.404362865633747 28.265760248514642 22.351576013816963 76-77 22.148005053693286 26.958548943748085 28.488209373347328 22.405236629211302 78-79 22.04362857131505 27.17795200089 28.339190885263648 22.4392285425313 80-81 22.257324784532702 27.280532350441973 27.966672920092257 22.49546994493307 82-83 22.201191037949272 27.215097957596363 28.090918280459377 22.492792723994988 84-85 22.075638341682126 27.335444035280766 27.923898204314355 22.66501941872275 86-87 22.400653263004568 27.442342918938007 27.535388757696804 22.621615060360618 88-89 22.044745358098382 27.32426148705944 27.47385999010328 23.157133164738898 90-91 21.952666509226788 27.23637832361841 27.846067938986895 22.964887228167907 92-93 22.145515363417626 26.862133791573438 28.076932074850397 22.915418770158542 94-95 21.790642765298436 26.65654959198438 28.371596798378445 23.181210844338743 96-97 21.934346581831086 26.301795988552847 28.331529635664843 23.432327793951227 98-99 21.81237232804784 26.687785689441785 28.019060970033177 23.480781012477202 100-101 22.077909467824895 26.019813236894674 28.66188710356534 23.24039019171509 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1687.0 1 1065.0 2 487.5 3 554.5 4 504.0 5 514.0 6 897.0 7 1920.5 8 3218.5 9 3746.5 10 3217.5 11 2544.5 12 2433.0 13 2562.0 14 2660.5 15 2703.5 16 2747.5 17 2889.5 18 3023.5 19 3153.0 20 3399.0 21 3718.0 22 4198.0 23 4813.0 24 5497.5 25 6443.5 26 7738.5 27 9157.0 28 10344.0 29 11781.5 30 13501.0 31 15817.5 32 19444.0 33 20867.5 34 20396.0 35 21348.0 36 23398.5 37 25301.5 38 27163.0 39 28669.5 40 29614.5 41 31226.0 42 32168.5 43 32652.0 44 33766.0 45 35895.5 46 37100.0 47 38650.0 48 41051.0 49 39994.0 50 37864.0 51 37130.5 52 36480.0 53 36954.5 54 38608.5 55 37897.5 56 35537.5 57 33033.0 58 31149.5 59 28364.0 60 23372.5 61 20055.5 62 17319.0 63 12307.0 64 8021.5 65 6058.5 66 4543.5 67 3409.5 68 2602.5 69 1971.0 70 1530.0 71 1064.0 72 690.5 73 568.0 74 430.0 75 309.5 76 253.0 77 167.5 78 95.0 79 59.0 80 44.5 81 28.5 82 19.0 83 15.0 84 13.0 85 8.0 86 5.0 87 6.5 88 5.5 89 3.0 90 2.5 91 2.5 92 3.0 93 1.5 94 0.0 95 1.5 96 2.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2755532213378827 2 0.25925090411770096 3 0.24717837731140424 4 0.2361632981085787 5 0.23369592036714582 6 0.19853578755172682 7 0.189371241654976 8 0.20029820022417888 9 0.2025893366983666 10-11 0.202501216064744 12-13 0.19963729547200934 14-15 0.19739021931463296 16-17 0.19624465107753913 18-19 0.19708179709695384 20-21 0.18359934015269544 22-23 0.1764615688292645 24-25 0.17787149896722618 26-27 0.1896796638726551 28-29 0.20298587954966832 30-31 0.19725803836419903 32-33 0.20153188909489533 34-35 0.19642089234478433 36-37 0.18210128938111117 38-39 0.18342309888545022 40-41 0.17712247358143404 42-43 0.19007620672395684 44-45 0.18668356232948657 46-47 0.18672762264629789 48-49 0.19487878125638874 50-51 0.2026774573319892 52-53 0.21259102861453213 54-55 0.1913098955946733 56-57 0.16487370550789207 58-59 0.16637175627947637 60-61 0.17293674348436036 62-63 0.1803829370254704 64-65 0.1694119181394562 66-67 0.1744347942559446 68-69 0.17778337833360358 70-71 0.1721877180985682 72-73 0.17505163869130286 74-75 0.1760209656611515 76-77 0.1913098955946733 78-79 0.19602434949348263 80-81 0.1846567877561667 82-83 0.1849211496570345 84-85 0.17707841326462273 86-87 0.17914924815475392 88-89 0.18589047662688313 90-91 0.1850973909242797 92-93 0.1822775306483564 94-95 0.18236565128197899 96-97 0.1718352355640778 98-99 0.1781799211849053 100-101 0.1699847022580031 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1134808.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.485387102625936 #Duplication Level Percentage of deduplicated Percentage of total 1 71.49006667073778 33.94733489852519 2 14.340293075486327 13.61908735709149 3 5.512122438436473 7.85235803238679 4 2.8128465307442445 5.342764254906754 5 1.6083354253441575 3.818621513166693 6 0.9873450694387824 2.81306776957018 7 0.6389628076559234 2.1238977386005566 8 0.45192415632497657 1.7167834803295319 9 0.3415272817354058 1.4595799661381998 >10 1.6516367731923751 13.788106521680815 >50 0.10368514732591073 3.3793651922822283 >100 0.05210378239435437 4.666954817606506 >500 0.005976059548307255 1.824410431895973 >1k 0.0026145260523844243 1.611490126302125 >5k 3.7350372176920345E-4 1.1406888341747876 >10k+ 1.8675186088460173E-4 0.8954890653422221 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10098 0.8898421583210551 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7861 0.6927163009072901 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5002 0.44077940938026516 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1980 0.17447885457275591 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1598 0.1408167725289212 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1485 0.13085914092956694 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 1397 0.1231045251707778 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1358 0.11966782045949624 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1304 0.11490930624387562 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1248 0.10997455076100979 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1200 0.1057447603471248 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1188 0.10468731274365356 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 1145 0.10089812549788157 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.8120633622604E-5 0.0 18-19 0.0 0.0 0.0 8.8120633622604E-5 0.0 20-21 0.0 0.0 0.0 8.8120633622604E-5 0.0 22-23 0.0 0.0 0.0 3.96542851301718E-4 0.0 24-25 0.0 0.0 0.0 8.8120633622604E-4 0.0 26-27 0.0 0.0 0.0 0.001586171405206872 0.0 28-29 0.0 0.0 0.0 0.00330452376084765 0.0 30-31 0.0 0.0 0.0 0.007666495125166548 0.0 32-33 0.0 0.0 0.0 0.013790879161937525 0.0 34-35 0.0 0.0 0.0 0.018769694961614652 0.0 36-37 0.0 0.0 0.0 0.021986098088839695 0.0 38-39 0.0 0.0 0.0 0.025422802800121255 0.0 40-41 0.0 0.0 0.0 0.029388231313138433 0.0 42-43 0.0 0.0 0.0 0.03401456457832515 0.0 44-45 0.0 0.0 0.0 0.03943398354611529 0.0 46-47 0.0 0.0 0.0 0.04591085011737668 0.0 48-49 0.0 0.0 0.0 0.05432637062833537 0.0 50-51 0.0 0.0 0.0 0.06463648476218004 0.0 52-53 0.0 0.0 0.0 0.09032364946316909 0.0 54-55 0.0 0.0 0.0 0.12204707756730654 0.0 56-57 0.0 0.0 0.0 0.15936616590647934 0.0 58-59 0.0 0.0 0.0 0.21056425404121226 0.0 60-61 0.0 0.0 0.0 0.2812370022065407 0.0 62-63 0.0 0.0 0.0 0.3290424459468033 0.0 64-65 0.0 0.0 0.0 0.3773325531719903 0.0 66-67 0.0 0.0 0.0 0.47250283748440264 0.0 68-69 0.0 0.0 0.0 0.5889542548166739 0.0 70-71 0.0 0.0 0.0 0.6892355358791972 0.0 72-73 0.0 0.0 0.0 0.7992541469570182 0.0 74-75 0.0 0.0 0.0 0.97122156347153 0.0 76-77 0.0 0.0 0.0 1.0986439996898154 0.0 78-79 0.0 0.0 0.0 1.2127602202310876 0.0 80-81 0.0 0.0 0.0 1.3586879895101198 0.0 82-83 0.0 0.0 0.0 1.5967458812415845 0.0 84-85 0.0 0.0 0.0 1.8459069728094972 0.0 86-87 0.0 0.0 0.0 2.133841143171356 0.0 88-89 0.0 0.0 0.0 2.496281309261126 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4935 0.0 46.90517 1 GTATCAA 8390 0.0 39.76986 1 TATCAAC 10745 0.0 30.963594 2 ATCAACG 11040 0.0 30.048784 3 TCAACGC 11065 0.0 29.97692 4 CAACGCA 11220 0.0 29.5628 5 AACGCAG 11495 0.0 28.885412 6 TAAGGTG 645 0.0 28.73346 5 AGGTAAG 610 0.0 27.270803 2 GGTAAGG 665 0.0 26.443602 3 ACGCAGA 12890 0.0 25.722483 7 CGCAGAG 12915 0.0 25.41523 8 GAGTACT 7550 0.0 24.881214 12-13 GTACATG 7930 0.0 24.155207 1 TACATGG 7970 0.0 23.85401 2 GTATAGT 200 2.0396328E-8 23.765608 1 GTACTTT 7810 0.0 23.68591 14-15 GCAGAGT 13700 0.0 23.577553 9 GTAAGGT 750 0.0 23.443554 4 AGTACTT 7635 0.0 23.204477 12-13 >>END_MODULE