FastQCFastQC Report
Thu 26 May 2016
SRR937805_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937805_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134808
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103350.9107267484896123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90420.7967867692155853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58950.5194711352052506No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC26140.23034733628948686No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23580.20778845408210025No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA22030.1941297558705966No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA17960.15826465798619677No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA16960.14945259462393637No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA14700.1295373314252279No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14490.1276867981191532No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA14000.12336888707164559No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13630.12010842362760925No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT12070.10636160478248304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54500.047.612991
AGGTAAG7000.040.036272
GTATCAA91750.038.279631
TAAGGTG9350.034.0379075
GGTAAGG8900.032.5566333
AAGGTAA9400.030.3356111
TATCAAC117250.029.9790942
GTAAGGT10050.029.7765334
TAAGGTA3850.029.6108024
TCAACGC120650.029.2130074
ATCAACG120800.029.1767313
CAACGCA122700.028.7636435
AACGCAG126100.028.0232986
GTATCGA700.00836933627.1575931
ACGCAGA140950.025.1393647
CGCAGAG141350.024.9674118
GAGTACT85700.024.35895212-13
GTGTAAG4500.024.290961
GGTGTGC12950.024.2077648
AAGGTGT13250.023.3001586
Produced by FastQC (version 0.11.5)