##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937805_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1134808 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24237580277897 33.0 31.0 34.0 31.0 34.0 2 32.43894650020091 34.0 31.0 34.0 31.0 34.0 3 32.48174845436409 34.0 31.0 34.0 31.0 34.0 4 36.1042334914805 37.0 35.0 37.0 35.0 37.0 5 36.03184855940388 37.0 35.0 37.0 35.0 37.0 6 36.09549280583147 37.0 35.0 37.0 35.0 37.0 7 36.05110027423141 37.0 35.0 37.0 35.0 37.0 8 36.016862764450025 37.0 35.0 37.0 35.0 37.0 9 37.6963556830759 39.0 37.0 39.0 35.0 39.0 10-11 37.62325388964477 39.0 37.0 39.0 35.0 39.0 12-13 37.51335732564452 39.0 37.0 39.0 35.0 39.0 14-15 38.68471186315218 40.0 38.0 41.0 34.5 41.0 16-17 38.65659697499489 40.0 38.0 41.0 34.5 41.0 18-19 38.69211575878916 40.0 38.0 41.0 34.0 41.0 20-21 38.61557021099604 40.0 38.0 41.0 34.0 41.0 22-23 38.55538514004131 40.0 38.0 41.0 34.0 41.0 24-25 38.5308602864978 40.0 38.0 41.0 34.0 41.0 26-27 38.33111548385277 40.0 38.0 41.0 34.0 41.0 28-29 38.267055308034486 40.0 38.0 41.0 34.0 41.0 30-31 38.183847399736344 40.0 37.5 41.0 34.0 41.0 32-33 37.964995840706095 40.0 37.0 41.0 33.5 41.0 34-35 37.78037782602872 40.0 36.5 41.0 33.0 41.0 36-37 37.54620076700199 40.0 36.0 41.0 33.0 41.0 38-39 37.337484402647846 40.0 36.0 41.0 33.0 41.0 40-41 37.10574828517247 40.0 36.0 41.0 32.0 41.0 42-43 36.79503669343184 39.5 35.0 41.0 31.0 41.0 44-45 36.35281651169185 39.0 35.0 41.0 30.5 41.0 46-47 35.835922905020055 39.0 35.0 41.0 29.0 41.0 48-49 35.3160732916934 39.0 35.0 40.5 26.5 41.0 50-51 34.968890772712214 39.0 35.0 40.0 25.5 41.0 52-53 34.63884991998647 38.0 34.5 40.0 24.0 41.0 54-55 34.36042088177031 38.0 34.0 40.0 23.5 41.0 56-57 34.04117745028233 38.0 34.0 40.0 23.0 41.0 58-59 33.7306773480624 37.0 34.0 40.0 22.0 41.0 60-61 33.345224919105256 37.0 33.0 40.0 20.5 41.0 62-63 32.90554349281993 36.0 33.0 39.0 18.5 41.0 64-65 32.48154181147824 35.5 32.0 39.0 17.5 40.0 66-67 32.04754636907741 35.0 32.0 38.5 13.0 40.0 68-69 31.56870633622604 35.0 31.0 38.0 8.0 40.0 70-71 31.124228063249465 35.0 31.0 37.0 7.0 39.5 72-73 30.668634694150903 35.0 30.5 37.0 4.0 39.0 74-75 30.215273420702005 34.0 30.0 36.0 2.0 39.0 76-77 29.01094942933078 33.5 28.5 35.0 2.0 37.0 78-79 29.598233798140303 34.0 30.0 35.0 2.0 37.0 80-81 29.44550884378679 34.0 30.0 35.0 2.0 37.0 82-83 29.072163308683056 34.0 29.0 35.0 2.0 36.5 84-85 28.69018767932549 34.0 29.0 35.0 2.0 36.0 86-87 28.389290963757745 34.0 29.0 35.0 2.0 36.0 88-89 27.99191625367463 34.0 28.0 35.0 2.0 35.0 90-91 27.506176815813774 33.0 27.0 35.0 2.0 35.0 92-93 27.04944052209713 33.0 26.0 35.0 2.0 35.0 94-95 26.576510299539656 33.0 25.0 35.0 2.0 35.0 96-97 26.067611877956445 33.0 24.5 35.0 2.0 35.0 98-99 25.28074661088043 32.0 21.0 34.0 2.0 35.0 100-101 22.923991547468823 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18028585632856675 1101 2 0.19149376477430735 1101 3 0.187076460958977 1101 4 0.12914828493116914 1101 5 0.0690689101989932 1101 6 0.0734639666764565 1101 7 0.025824187489227768 1101 8 0.2149230209327584 1101 9 0.17426240644412871 1101 10-11 0.14379808689829332 1101 12-13 0.20278838534505184 1101 14-15 0.2309440575730335 1101 16-17 0.302116193422755 1101 18-19 0.3500797440412029 1101 20-21 0.34341566596585693 1101 22-23 0.22551222388790393 1101 24-25 0.12181607773010938 1101 26-27 0.1774896208453498 1101 28-29 0.1544962604041018 1101 30-31 0.21410976876482124 1101 32-33 0.09160950255698452 1101 34-35 0.04369539484009266 1101 36-37 -0.07387731444734413 1101 38-39 0.09512653674195093 1101 40-41 0.06152376553553296 1101 42-43 0.0163883586514757 1101 44-45 0.017752781041401988 1101 46-47 0.0030592440621362016 1101 48-49 -0.2166448945730579 1101 50-51 -0.009247090424558735 1101 52-53 0.114450216210777 1101 54-55 0.037056725366085175 1101 56-57 -0.05324521532507731 1101 58-59 -0.020500793302552722 1101 60-61 0.18152277673436146 1101 62-63 0.12989020339821877 1101 64-65 0.24376030801682447 1101 66-67 0.1810992696773539 1101 68-69 0.16441566411247166 1101 70-71 0.10247628201380721 1101 72-73 0.1147533957654332 1101 74-75 0.09411090084277873 1101 76-77 0.05025020272087133 1101 78-79 0.23380463557312936 1101 80-81 0.4917326104724111 1101 82-83 0.3574156417923362 1101 84-85 0.5681111585513214 1101 86-87 0.48170873441866746 1101 88-89 0.6200632301179887 1101 90-91 0.4919216148963592 1101 92-93 0.6322997401121135 1101 94-95 0.5228025967997354 1101 96-97 0.5687027807333358 1101 98-99 0.5541727058952723 1101 100-101 1.2125306592165792 1102 1 0.0682885672322584 1102 2 0.16309027140523824 1102 3 0.06449494131262412 1102 4 0.12645155187816925 1102 5 -0.03222831882615651 1102 6 0.04734872246408628 1102 7 0.04769255463183697 1102 8 0.028703037259795394 1102 9 0.16664969600252988 1102 10-11 0.19203553741659363 1102 12-13 0.1954233529220275 1102 14-15 0.173863516008403 1102 16-17 0.18262171534873062 1102 18-19 0.15083633261536278 1102 20-21 0.19606757669085795 1102 22-23 0.041973262348854234 1102 24-25 0.07023035310246684 1102 26-27 0.019848107052084174 1102 28-29 0.026010515752744823 1102 30-31 0.16568378721745347 1102 32-33 0.16725388128254792 1102 34-35 0.12636686719965695 1102 36-37 0.20227197008972553 1102 38-39 0.16427984142595875 1102 40-41 0.2828564211429452 1102 42-43 0.44651943700583274 1102 44-45 0.4454323399034408 1102 46-47 0.09903570848921817 1102 48-49 0.14873440090004664 1102 50-51 0.05672767968771808 1102 52-53 0.1541008793039893 1102 54-55 0.09372447023307728 1102 56-57 0.05855214462440017 1102 58-59 0.16899753548694463 1102 60-61 0.23363035032152624 1102 62-63 0.049846459270568744 1102 64-65 0.3767868163420687 1102 66-67 0.1376644314844171 1102 68-69 0.2816961348239282 1102 70-71 0.09388240927225056 1102 72-73 0.19081303564652075 1102 74-75 0.38619660236611963 1102 76-77 0.3650578979906527 1102 78-79 0.2320847520187641 1102 80-81 0.36316923539732215 1102 82-83 0.4692573619839493 1102 84-85 0.5563301256354087 1102 86-87 0.3446097094198244 1102 88-89 0.3559321825693509 1102 90-91 0.21227680489066003 1102 92-93 0.2872580753708043 1102 94-95 0.5371214052343376 1102 96-97 0.716320420090728 1102 98-99 0.6320742199965927 1102 100-101 1.162890932155289 1103 1 0.16687980193606222 1103 2 0.18068659553293998 1103 3 0.0962803877939038 1103 4 0.047491134513499844 1103 5 0.05961998762416698 1103 6 0.1056304436147073 1103 7 0.07838098297307283 1103 8 0.11522451973398518 1103 9 0.035561452731258214 1103 10-11 0.06685708849430938 1103 12-13 0.2036746013465418 1103 14-15 0.2709027157403696 1103 16-17 0.18113987932709819 1103 18-19 0.1599069942404654 1103 20-21 0.20559134063093865 1103 22-23 0.29651944209006587 1103 24-25 0.12342679848290317 1103 26-27 0.09436875990232352 1103 28-29 0.19357285720681716 1103 30-31 0.1863219594920551 1103 32-33 0.1137211191134 1103 34-35 6.239549004760647E-4 1103 36-37 0.06689776182220442 1103 38-39 0.23772198313211135 1103 40-41 0.21052214650550383 1103 42-43 0.3002152521911441 1103 44-45 0.3314943383399154 1103 46-47 0.27715214302072155 1103 48-49 0.1463418930425533 1103 50-51 0.13469733459606203 1103 52-53 -0.05007468188875919 1103 54-55 0.0061126704277185695 1103 56-57 -0.008018158028804123 1103 58-59 0.07231603131489095 1103 60-61 0.1256908490531643 1103 62-63 0.23300413568531297 1103 64-65 0.28249622459683366 1103 66-67 0.18138178498400492 1103 68-69 0.35545130003077574 1103 70-71 0.3783575923479212 1103 72-73 0.48336779880483505 1103 74-75 0.5729522940650966 1103 76-77 0.30789393557899913 1103 78-79 0.368257632887957 1103 80-81 0.4268039455873378 1103 82-83 0.31294025418069893 1103 84-85 0.2800379065508629 1103 86-87 0.5436514937495041 1103 88-89 0.5853812803532996 1103 90-91 0.5354829823982783 1103 92-93 0.7302807783494778 1103 94-95 0.7944522496958015 1103 96-97 0.7906923788009834 1103 98-99 0.7444595484159997 1103 100-101 1.1396212565063486 1104 1 0.13037471316604154 1104 2 0.1514798567475708 1104 3 0.1085708011647597 1104 4 0.1255547341346741 1104 5 0.15601983289354848 1104 6 0.035651518992530384 1104 7 0.14559676549651357 1104 8 0.1078801507281284 1104 9 0.13673327041590255 1104 10-11 0.15009082510543692 1104 12-13 0.21249824845401122 1104 14-15 0.26300044947031864 1104 16-17 0.22233724090322227 1104 18-19 0.20248692727945183 1104 20-21 0.10982842529259784 1104 22-23 0.23581768536396197 1104 24-25 0.15968711941555824 1104 26-27 0.27938523337802934 1104 28-29 0.3419651927054943 1104 30-31 0.3363989913318832 1104 32-33 0.22596651387657118 1104 34-35 0.4061852535705981 1104 36-37 0.32743911891646604 1104 38-39 0.34429269902801707 1104 40-41 0.39715728903107816 1104 42-43 0.39503277676796955 1104 44-45 0.4880617195241399 1104 46-47 0.4138749884635118 1104 48-49 0.22290187116684024 1104 50-51 0.2767180364861801 1104 52-53 0.3118299053406304 1104 54-55 0.3672522747975293 1104 56-57 0.3569160147626178 1104 58-59 0.28140512147215446 1104 60-61 0.23206815167157657 1104 62-63 0.3061016826713967 1104 64-65 0.5731593653616756 1104 66-67 0.5348325446313211 1104 68-69 0.5204698410277224 1104 70-71 0.4906856879607737 1104 72-73 0.5490852953700838 1104 74-75 0.567188244924786 1104 76-77 0.6254018388456828 1104 78-79 0.6377887057313991 1104 80-81 0.7461055902671418 1104 82-83 0.5565122639447324 1104 84-85 0.6331125107205899 1104 86-87 0.729440953392821 1104 88-89 0.6332417293388808 1104 90-91 0.6581093346238696 1104 92-93 0.7858614183473414 1104 94-95 0.8805511705831037 1104 96-97 1.084783936644893 1104 98-99 1.1368797487654803 1104 100-101 1.3208931822356504 1105 1 0.14040566581994085 1105 2 0.17517583290256766 1105 3 0.17509894515192315 1105 4 0.09005089371280661 1105 5 0.17215533673397232 1105 6 0.10768808897991988 1105 7 0.20204810104620918 1105 8 0.24793517766840267 1105 9 0.2783273320919335 1105 10-11 0.2360818832276479 1105 12-13 0.36859420168111257 1105 14-15 0.3005778311050804 1105 16-17 0.4141591485038134 1105 18-19 0.4600273376887145 1105 20-21 0.45052973218243153 1105 22-23 0.32563053644729933 1105 24-25 0.23023480943046337 1105 26-27 0.31389171050745546 1105 28-29 0.150865513695976 1105 30-31 0.286602476371435 1105 32-33 0.2646387023438379 1105 34-35 0.25637440867778594 1105 36-37 0.41228808142936657 1105 38-39 0.3805330979733341 1105 40-41 0.533906400575269 1105 42-43 0.42949626410030817 1105 44-45 0.4165309961491559 1105 46-47 0.4078979736750128 1105 48-49 0.5692993261708565 1105 50-51 0.554913611403073 1105 52-53 0.6666605255401024 1105 54-55 0.7947370850692224 1105 56-57 0.6052465776423617 1105 58-59 0.7304880058009431 1105 60-61 0.6763247147805984 1105 62-63 0.7943110143233554 1105 64-65 0.8195754492777567 1105 66-67 0.7750437678343474 1105 68-69 0.6794045538558784 1105 70-71 0.6208229687128117 1105 72-73 0.6783857080721347 1105 74-75 0.695161705890051 1105 76-77 0.5041419511923522 1105 78-79 0.8052110374979904 1105 80-81 0.8507628182850269 1105 82-83 0.8658654107978805 1105 84-85 0.6857621152144553 1105 86-87 0.8651712067460267 1105 88-89 0.9568001381431905 1105 90-91 1.0610125789205505 1105 92-93 1.1634307752199753 1105 94-95 1.027791225610045 1105 96-97 1.0565038151270691 1105 98-99 1.3182236098224536 1105 100-101 1.5064529093937402 1106 1 -0.18927307128453918 1106 2 -0.07513919860058849 1106 3 -0.16458290764874306 1106 4 -0.15239527398088626 1106 5 -0.09702350422705308 1106 6 -0.1502517434648638 1106 7 -0.16081453137088175 1106 8 -0.04586112503879036 1106 9 -0.4412167376996621 1106 10-11 -0.3602727522359004 1106 12-13 -0.4780633486893109 1106 14-15 -0.33463386314127064 1106 16-17 -0.17214823223425668 1106 18-19 -0.1878920696366322 1106 20-21 -0.18554570228409517 1106 22-23 -0.14146684636790496 1106 24-25 -0.2319051545659434 1106 26-27 -0.06105463028047353 1106 28-29 -0.32447288552856435 1106 30-31 -0.5896316855062977 1106 32-33 -0.740544604448381 1106 34-35 -0.7765083026702726 1106 36-37 -0.8173222834532794 1106 38-39 -0.7646912321519892 1106 40-41 -0.6894033342443606 1106 42-43 -0.9057422597520741 1106 44-45 -0.9583106226281117 1106 46-47 -0.9347169608953649 1106 48-49 -1.0749677928487387 1106 50-51 -0.9988459953268389 1106 52-53 -1.0513213688031797 1106 54-55 -0.9035925555871529 1106 56-57 -1.068071811888899 1106 58-59 -1.2504647048547426 1106 60-61 -1.169701041641197 1106 62-63 -1.4385907373902782 1106 64-65 -1.6825337039452535 1106 66-67 -1.7630655238640536 1106 68-69 -1.7425515840656054 1106 70-71 -1.5152265016188373 1106 72-73 -1.455642021623909 1106 74-75 -1.3943236849567313 1106 76-77 -1.5261806964570255 1106 78-79 -1.442324100631204 1106 80-81 -1.793656623659171 1106 82-83 -1.903388726154276 1106 84-85 -1.6536834639588456 1106 86-87 -1.5684246528399335 1106 88-89 -1.8035785920375211 1106 90-91 -2.2583774370041176 1106 92-93 -1.8906724235614405 1106 94-95 -1.7167788083164268 1106 96-97 -1.8339630200179862 1106 98-99 -1.9672754208498446 1106 100-101 -1.4060626690871878 1107 1 0.18893641477164635 1107 2 0.24750341177831814 1107 3 0.17372438425911696 1107 4 0.1878107110703766 1107 5 0.13427436831043593 1107 6 0.1831810011192303 1107 7 0.17925622968090238 1107 8 0.14280784475809583 1107 9 0.13781057569585187 1107 10-11 0.1898407062568097 1107 12-13 0.1876550519627287 1107 14-15 0.31356747242727323 1107 16-17 0.3103078431207038 1107 18-19 0.3936771770916039 1107 20-21 0.2195793799154231 1107 22-23 0.31460665621153794 1107 24-25 0.30911587924931894 1107 26-27 0.13331643919317315 1107 28-29 0.398716177998935 1107 30-31 0.23403390369060872 1107 32-33 0.21270262392273054 1107 34-35 0.29914443098475374 1107 36-37 0.22433180244174622 1107 38-39 0.44322076110533715 1107 40-41 0.3236267947741496 1107 42-43 0.4775233072162166 1107 44-45 0.4366240345420067 1107 46-47 0.30685793560270724 1107 48-49 0.3459581210709999 1107 50-51 0.3607072097598092 1107 52-53 0.44740743298252283 1107 54-55 0.5266355654475916 1107 56-57 0.6350179638800739 1107 58-59 0.5143376921295797 1107 60-61 0.5467333052546763 1107 62-63 0.4799266148126691 1107 64-65 0.581367971535677 1107 66-67 0.7897996503989262 1107 68-69 0.81284857709176 1107 70-71 0.6676148695154716 1107 72-73 0.6362162895580035 1107 74-75 0.7299897753883684 1107 76-77 0.7431961617152254 1107 78-79 0.9986978593758309 1107 80-81 1.0208878289151784 1107 82-83 0.9121249861149892 1107 84-85 0.8292181096409834 1107 86-87 1.0787416634175102 1107 88-89 1.147886180258233 1107 90-91 1.1414141698459979 1107 92-93 1.010380656489314 1107 94-95 1.2480962947224086 1107 96-97 1.2930489000701861 1107 98-99 1.1144234927272016 1107 100-101 1.585446004698806 1108 1 0.19646272662985353 1108 2 0.21734658990222044 1108 3 0.1839432377414596 1108 4 0.2379277317037065 1108 5 0.15843521654996806 1108 6 0.13031897520396285 1108 7 0.1446648948987459 1108 8 0.12040586287672994 1108 9 0.23941045049443943 1108 10-11 0.2611423740676031 1108 12-13 0.3398045502678855 1108 14-15 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-0.5731444741035148 2316 54-55 -0.5932015617878861 2316 56-57 -0.31781896100925167 2316 58-59 -0.35574654107913517 2316 60-61 -0.274021371748951 2316 62-63 -0.25416881841469063 2316 64-65 -0.2513876335058214 2316 66-67 -0.2741819922492823 2316 68-69 -0.16894450648355885 2316 70-71 -0.4115083042045313 2316 72-73 -0.3459804269253688 2316 74-75 -0.2618432794982901 2316 76-77 -0.2765484795547053 2316 78-79 -0.50220210428661 2316 80-81 -0.5246103928752746 2316 82-83 -0.32055455372231023 2316 84-85 0.028540726750147627 2316 86-87 0.154692981686825 2316 88-89 0.06239810755769426 2316 90-91 0.0317753948136037 2316 92-93 0.22684548904522117 2316 94-95 0.28552768507356774 2316 96-97 0.426646625318007 2316 98-99 0.08162010856719704 2316 100-101 0.5960834798425445 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 8.0 5 26.0 6 72.0 7 178.0 8 433.0 9 735.0 10 1400.0 11 2304.0 12 3565.0 13 5070.0 14 6449.0 15 9150.0 16 13088.0 17 18549.0 18 16995.0 19 9835.0 20 7845.0 21 7446.0 22 7344.0 23 7819.0 24 8681.0 25 10236.0 26 12070.0 27 14552.0 28 18018.0 29 21998.0 30 26680.0 31 33572.0 32 42492.0 33 55685.0 34 78525.0 35 116382.0 36 174782.0 37 238444.0 38 153487.0 39 10887.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.197342797775995 19.207458714278033 15.679185719411365 20.916012768534607 2 16.043595039865775 21.011836363508188 39.22954367611085 23.715024920515187 3 19.806081733650096 25.543616188817843 30.20202536464318 24.448276712888877 4 13.386241008382923 17.184067422918613 35.69831698253535 33.73137458616311 5 13.276138606880995 38.15743090806543 32.9590559785674 15.607374506486183 6 31.25956108874805 36.53305228725917 16.30328654715159 15.904100076841193 7 27.95265807079259 34.98195289423409 19.308640756850497 17.75674827812282 8 28.15418995988749 33.25223297685599 18.763702758528314 19.8298743047282 9 27.94587278200365 16.128190848143475 19.508762715807432 36.41717365404544 10-11 25.71307216727411 27.104849454709516 25.076224348083553 22.105854029932818 12-13 27.29125358008372 24.768803701255784 26.398898435778804 21.541044282881693 14-15 23.649110686565482 25.425622660397178 26.14292461808517 24.78234203495217 16-17 21.57259201556563 28.65616914931865 27.111855045082518 22.659383790033203 18-19 21.72028219751711 25.706463119752414 31.4932570091152 21.07999767361527 20-21 22.673068356708136 24.472232882403976 32.038470062464505 20.816228698423384 22-23 22.517406288911353 24.566021408347442 32.00637826701128 20.91019403572993 24-25 22.00654207584014 24.9797322542668 32.03572762969595 20.977998040197107 26-27 21.146768476314058 25.30447062141043 31.900272956188218 21.648487946087297 28-29 21.447297343739947 25.57299097366828 31.872476958312145 21.107234724279625 30-31 21.370707643936242 25.511055614694296 31.488718796483635 21.62951794488583 32-33 21.095639086083285 25.401037003616473 31.573931449196692 21.929392461103554 34-35 21.81562872309677 25.523392503401453 31.391962340766018 21.269016432735757 36-37 22.320947053411867 24.970876027930814 31.163046658277555 21.545130260379768 38-39 21.6816412996736 25.4097168860283 31.056663329832006 21.851978484466095 40-41 21.966344041913754 25.439755588287515 31.127950007336054 21.465950362462674 42-43 21.870791349659076 25.561623454918525 30.529197015956733 22.038388179465667 44-45 21.769365390444904 25.888211926599038 30.500489950722944 21.84193273223312 46-47 22.551039470994212 26.43041818527892 29.256006302387718 21.76253604133915 48-49 22.41634708250206 26.579606418001987 28.66083954290065 22.343206956595303 50-51 22.52495576344192 27.079074169374906 28.505923468992112 21.89004659819106 52-53 22.997458600926322 26.753160005921707 28.35431191884442 21.895069474307547 54-55 22.380922587785776 27.35057384156615 28.337613058772938 21.930890511875138 56-57 22.624179596900973 27.46182614151469 27.817040415647405 22.096953845936934 58-59 22.37770618465855 26.823171849334866 27.885113605120864 22.914008360885717 60-61 22.09950934431199 27.29884702962968 28.41969742899239 22.181946197065937 62-63 22.33880092491417 27.33665078145378 28.57329169339659 21.751256600235457 64-65 22.099729645896048 27.260734855587902 28.47027867269177 22.16925682582428 66-67 22.013900148747627 27.35647792401886 28.399077200724705 22.230544726508803 68-69 22.66942954226618 27.2076862341471 28.167363994614064 21.955520228972656 70-71 22.609948114570923 27.484693445939754 27.569289254217455 22.33606918527187 72-73 22.354486397698995 27.5928615237115 27.501348245694423 22.55130383289508 74-75 22.35338488977871 28.03042452996454 27.36211764457071 22.254072935686036 76-77 22.508256903370437 27.289153759931196 28.084486538692005 22.11810279800636 78-79 22.414981212680914 27.697945379306454 27.629079104130387 22.257994303882242 80-81 22.635106555470177 27.95138032160506 27.321582153104313 22.091930969820446 82-83 22.54191032107747 28.058633913874115 27.1931981205565 22.206257644491913 84-85 22.389372966609212 28.19125593494559 27.23267630357313 22.186694794872068 86-87 22.64127499982376 28.22772662864555 26.757477916969215 22.373520454561476 88-89 22.23146999316184 28.164147591486845 26.686893289437506 22.91748912591381 90-91 22.144847962738147 28.212678107559668 26.96452059955626 22.67795333014592 92-93 22.32134634915809 27.64338226384584 27.306310295676006 22.728961091320066 94-95 21.93172765789455 27.539901022904317 27.59797252046161 22.930398798739525 96-97 22.30434575716773 27.17622716794383 27.66631007183594 22.853117003052496 98-99 21.865748681821408 27.4804602907373 27.157717474683967 23.496073552757323 100-101 21.62001259424199 27.67952633663995 27.739324749448173 22.96113631966989 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 857.0 1 619.0 2 461.5 3 560.5 4 526.5 5 526.5 6 929.5 7 2050.0 8 3193.5 9 3656.5 10 3253.0 11 2681.5 12 2697.5 13 2790.0 14 2842.5 15 2904.0 16 3025.0 17 3147.5 18 3252.5 19 3413.0 20 3594.5 21 3866.5 22 4329.5 23 4848.0 24 5530.0 25 6538.0 26 7677.0 27 8963.0 28 10208.5 29 11718.5 30 13580.0 31 15637.5 32 18933.0 33 20381.5 34 19981.5 35 20870.0 36 23129.5 37 25193.5 38 26755.0 39 28344.0 40 29372.0 41 30737.0 42 31433.0 43 32406.0 44 33815.0 45 35382.0 46 35959.5 47 37374.5 48 40660.5 49 39880.0 50 38085.0 51 37542.0 52 36493.0 53 37487.5 54 40283.5 55 39500.0 56 36044.5 57 33721.0 58 32263.0 59 29332.5 60 23507.0 61 19685.0 62 17242.0 63 12196.5 64 7827.0 65 5837.0 66 4369.0 67 3465.5 68 2694.5 69 2003.5 70 1491.5 71 997.5 72 703.5 73 568.5 74 427.5 75 303.5 76 235.5 77 173.0 78 95.5 79 55.5 80 47.5 81 29.0 82 17.0 83 13.0 84 5.0 85 2.5 86 2.5 87 1.0 88 0.0 89 0.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04027112956553003 2 0.0 3 0.0 4 8.8120633622604E-5 5 0.007754615758789152 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005110996750111032 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.08422217679114E-4 22-23 0.001498050771584268 24-25 0.0 26-27 9.25266653037342E-4 28-29 0.001498050771584268 30-31 0.0 32-33 0.0 34-35 0.0 36-37 3.52482534490416E-4 38-39 0.0 40-41 1.32180950433906E-4 42-43 0.02114895206942496 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.52482534490416E-4 84-85 3.52482534490416E-4 86-87 0.0 88-89 0.0 90-91 0.011940345855862842 92-93 0.003128282493602442 94-95 0.0 96-97 0.0 98-99 3.08422217679114E-4 100-101 0.014319602963673149 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1134808.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.35884847272293 #Duplication Level Percentage of deduplicated Percentage of total 1 69.88411749132412 31.69863095938937 2 14.840279873410875 13.462760121416876 3 5.9447334559702565 8.089387921202443 4 3.0109229313622516 5.462879880268286 5 1.6693396310696793 3.7859661687600403 6 1.0681223567878035 2.9069280079119415 7 0.7231345844238536 2.296038642818693 8 0.5085561617960059 1.845401750621968 9 0.3658686859470732 1.4935844058108794 >10 1.8064088832567566 14.420840353453368 >50 0.11202606377225706 3.5201010612706667 >100 0.05609470871595927 4.723048550659155 >500 0.007060871657977681 2.1071294959636466 >1k 0.002745894533657987 1.9389861669633808 >5k 3.922706476654268E-4 1.328866087448131 >10k+ 1.961353238327134E-4 0.9194504260411351 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10335 0.9107267484896123 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9042 0.7967867692155853 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5895 0.5194711352052506 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2614 0.23034733628948686 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2358 0.20778845408210025 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2203 0.1941297558705966 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1796 0.15826465798619677 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1696 0.14945259462393637 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1470 0.1295373314252279 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1449 0.1276867981191532 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 1400 0.12336888707164559 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1363 0.12010842362760925 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 1207 0.10636160478248304 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.8120633622604E-5 0.0 18-19 0.0 0.0 0.0 8.8120633622604E-5 0.0 20-21 0.0 0.0 0.0 8.8120633622604E-5 0.0 22-23 0.0 0.0 0.0 3.96542851301718E-4 0.0 24-25 0.0 0.0 0.0 8.8120633622604E-4 0.0 26-27 0.0 0.0 0.0 0.001586171405206872 0.0 28-29 0.0 0.0 0.0 0.003568885661715462 0.0 30-31 0.0 0.0 0.0 0.008018977659656965 0.0 32-33 0.0 0.0 0.0 0.014407723597295754 0.0 34-35 0.0 0.0 0.0 0.019562780664218085 0.0 36-37 0.0 0.0 0.0 0.022823244108254436 0.0 38-39 0.0 0.0 0.0 0.026348069453158596 0.0 40-41 0.0 0.0 0.0 0.03040161859979838 0.0 42-43 0.0 0.0 0.0 0.0353363740826642 0.0 44-45 0.0 0.0 0.0 0.04115233590175607 0.0 46-47 0.0 0.0 0.0 0.04789356437388527 0.0 48-49 0.0 0.0 0.0 0.056353145201655255 0.0 50-51 0.0 0.0 0.0 0.06670731965231122 0.0 52-53 0.0 0.0 0.0 0.0927029065709794 8.8120633622604E-5 54-55 0.0 0.0 0.0 0.12442633467511685 8.8120633622604E-5 56-57 0.0 0.0 0.0 0.16192166428153487 8.8120633622604E-5 58-59 0.0 0.0 0.0 0.21294351114902257 8.8120633622604E-5 60-61 0.0 0.0 0.0 0.2842331037497092 8.8120633622604E-5 62-63 0.0 0.0 0.0 0.3329197538261979 8.8120633622604E-5 64-65 0.0 0.0 0.0 0.3807251975664606 8.8120633622604E-5 66-67 0.0 0.0 0.0 0.47536675807713724 8.8120633622604E-5 68-69 0.0 0.0 0.0 0.5917300547757858 8.8120633622604E-5 70-71 0.0 0.0 0.0 0.6921875771055543 8.8120633622604E-5 72-73 0.0 0.0 0.0 0.8033517564204693 8.8120633622604E-5 74-75 0.0 0.0 0.0 0.9766409824393202 8.8120633622604E-5 76-77 0.0 0.0 0.0 1.106310494814982 8.8120633622604E-5 78-79 0.0 0.0 0.0 1.221263861375669 8.8120633622604E-5 80-81 0.0 0.0 0.0 1.3683812592086062 8.8120633622604E-5 82-83 0.0 0.0 0.0 1.6110654842052576 8.8120633622604E-5 84-85 0.0 0.0 0.0 1.8647207280879232 8.8120633622604E-5 86-87 0.0 0.0 0.0 2.1583827396352513 8.8120633622604E-5 88-89 0.0 0.0 0.0 2.529855270671338 8.8120633622604E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5450 0.0 47.61299 1 AGGTAAG 700 0.0 40.03627 2 GTATCAA 9175 0.0 38.27963 1 TAAGGTG 935 0.0 34.037907 5 GGTAAGG 890 0.0 32.556633 3 AAGGTAA 940 0.0 30.335611 1 TATCAAC 11725 0.0 29.979094 2 GTAAGGT 1005 0.0 29.776533 4 TAAGGTA 385 0.0 29.610802 4 TCAACGC 12065 0.0 29.213007 4 ATCAACG 12080 0.0 29.176731 3 CAACGCA 12270 0.0 28.763643 5 AACGCAG 12610 0.0 28.023298 6 GTATCGA 70 0.008369336 27.157593 1 ACGCAGA 14095 0.0 25.139364 7 CGCAGAG 14135 0.0 24.967411 8 GAGTACT 8570 0.0 24.358952 12-13 GTGTAAG 450 0.0 24.29096 1 GGTGTGC 1295 0.0 24.207764 8 AAGGTGT 1325 0.0 23.300158 6 >>END_MODULE