FastQCFastQC Report
Thu 26 May 2016
SRR937804_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937804_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953018
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90000.9443683120360791No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71080.7458411068836056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45450.4769059975782199No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17910.18792929409517972No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA14380.15088907030087576No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12550.1316869146228088No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT11950.12539112587590162No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC11020.11563265331819546No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG11010.11552772350574701No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11000.11542279369329855No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA10930.11468828500615937No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA10340.10849742607170064No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA10160.10660868944762848No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA9940.10430023357376252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41650.049.437461
GTATCAA74450.041.2620771
TATCAAC94000.032.5047342
ATCAACG95550.031.8779833
TCAACGC96700.031.5513364
CAACGCA98600.030.9899185
AACGCAG101200.030.371986
ACGCAGA112700.027.1884947
CGCAGAG113400.026.9368788
GAGTACT68450.025.64022612-13
GCAGAGT118100.025.502859
TAAGGTA2501.4551915E-1124.7122634
GTACTTT71800.024.17803414-15
GTACATG66650.024.044351
TACATGG66050.023.3096472
AGTACTT69600.022.75974812-13
TAAGGTG4750.022.0097665
ACATGGG66900.021.9480463
CAGAGTA114150.021.69997610-11
AGAGTAC108400.021.40504610-11
Produced by FastQC (version 0.11.5)