FastQCFastQC Report
Thu 26 May 2016
SRR937804_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937804_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953018
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86420.9068034391795328No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75480.7920102243609249No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48070.5043976084397147No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24940.26169495224644235No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA21040.2207723253915456No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19880.20860046714752503No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA15790.16568417385610765No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA14460.15172850880046337No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA13850.14532779024110773No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12890.13525452824605622No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12850.1348348089962624No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA12480.13095240593566962No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT11530.12098407375306656No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG10600.11122560119536042No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA10050.10545446151069549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48200.049.688141
GTATCAA82700.039.532311
AGGTAAG5550.035.9472732
TATCAAC104100.031.16592
ATCAACG106300.030.47623
TCAACGC106500.030.4635684
CAACGCA108100.029.9687335
GGTAAGG7300.029.9326133
AAGGTAA6300.029.4166531
AACGCAG111150.029.143326
GTAAGGT7850.029.0456624
TAAGGTG8550.028.889965
ACGCAGA123900.025.880047
CGCAGAG124950.025.5865238
GAGTACT75350.025.4070512-13
GTACTTT77550.024.52928214-15
GCAGAGT133000.023.7521259
GGTGTGC10750.023.42078
GTGTGCA10900.023.0983969
AGTACTT76650.022.96193712-13
Produced by FastQC (version 0.11.5)